公开(公告)号：WO2008002563A2

公开(公告)日：2008-01-03

申请号：PCT/US2007014811

申请日：2007-06-26

Applicant: APPLERA CORP ,  HIRANO KIRK M ,  BABCOKE JASON E ,  CARRILLO ALBERT L ,  HAGA DOUWE D ,  KAO PIN ,  KINNEY PATRICK D ,  NURSE JAMES C

Inventor： HIRANO KIRK M ,  BABCOKE JASON E ,  CARRILLO ALBERT L ,  HAGA DOUWE D ,  KAO PIN ,  KINNEY PATRICK D ,  NURSE JAMES C

IPC: H05B3/20

CPC classification number: B01L7/52 ,  B01L3/50851 ,  B01L3/50853 ,  B01L3/50857 ,  B01L7/00 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/1816 ,  B01L2300/1838 ,  B01L2300/1861 ,  B01L2300/1872 ,  F28F13/00 ,  F28F2013/001 ,  G01N21/0332 ,  G01N21/6452 ,  G01N2201/062 ,  H05B3/84 ,  H05B6/02 ,  H05B6/105

Abstract: A heating apparatus comprising a support base and a microplate having a first surface and an opposing second surface. The microplate is positioned adjacent the support base and comprises a plurality of wells formed in the first surface thereof. Each of the plurality of wells is sized to receive an assay therein. A sapphire crystalline transparent window is positioned adjacent the microplate opposing the support base. A heating device heats the transparent window in response to a control system.

Abstract translation: 一种加热设备，包括支撑基座和具有第一表面和相对的第二表面的微孔板。 微孔板位于支撑底座附近并且包括在其第一表面中形成的多个阱。 多个孔中的每一个的大小适于在其中接受测定。 蓝宝石结晶透明窗口位于与支撑基座相对的微孔板附近。 加热装置响应于控制系统加热透明窗口。

公开(公告)号：WO2005072874A1

公开(公告)日：2005-08-11

申请号：PCT/US2005/001699

申请日：2005-01-21

Applicant: APPLERA CORPORATION ,  FLATBUSH, Michael, R. ,  HAGA, Douwe, D.

Inventor： FLATBUSH, Michael, R. ,  HAGA, Douwe, D.

IPC: B01L3/00

CPC classification number: B29C59/007 ,  B01J2219/00533 ,  B01J2219/00641 ,  B01L3/5023 ,  B01L3/5085 ,  B01L2200/12 ,  B01L2300/069 ,  B01L2300/0819 ,  Y10T436/25

Abstract: The present application relates to an apparatus and method for non-contact collapsing of a porous material on a nonporous substrate.

Abstract translation: 本申请涉及一种用于在无孔基底上非多孔材料非接触塌缩的装置和方法。

公开(公告)号：WO2004081227A2

公开(公告)日：2004-09-23

申请号：PCT/US2004007103

申请日：2004-03-09

Applicant: APPLERA CORP ,  OLDHAM MARK F ,  KING HOWARD G ,  HAGA DOUWE D ,  FEREA TRACY L

Inventor： OLDHAM MARK F ,  KING HOWARD G ,  HAGA DOUWE D ,  FEREA TRACY L

IPC: G01N21/64 ,  C12Q

CPC classification number: G01N21/76 ,  G01J3/10 ,  G01J3/4406 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2201/062 ,  G01N2201/0627 ,  G01N2201/0633 ,  G01N2201/0634

Abstract: An apparatus for imaging an array of a plurality of features associated with a sample tile. The apparatus includes a stage that supports the sample tile in an illumination region, and an illumination source having a plurality of LEDs adapted to emit light. At least a portion of the light illuminates the illumination region. Additionally, the apparatus includes an image collecting device adapted to selectively collect images of either a first signal when the illumination source is illuminating the illumination region, or a second signal absent illumination of the illumination region. The first signal has wavelengths effectively different from the wavelengths of the portion of the light emitted by the LEDs that illuminates the illumination region.

Abstract translation: 一种用于对与样本瓦片相关联的多个特征的阵列进行成像的装置。 该装置包括在照明区域中支撑样品瓦的台，以及具有适于发光的多个LED的照明源。 至少一部分光照射照明区域。 另外，该装置包括图像采集装置，适于在照明源照射照明区域时选择性地收集第一信号的图像，或者不存在照明区域的照明的第二信号。 第一信号具有与照亮照明区域的由LED发射的光的部分的波长有效不同的波长。

公开(公告)号：WO2009079052A2

公开(公告)日：2009-06-25

申请号：PCT/US2008/077092

申请日：2008-09-19

Applicant: APPLIED BIOSYSTEMS INC. ,  YANG, Joon Mo ,  LIU, David, M. ,  CHIANG, Yuh-Min ,  SCHEMBRI, Carol ,  LAU, Aldrich, N. K. ,  ULMANELLA, Umberto ,  BEARD, Nigel, P. ,  SHARIATI, Maryam ,  NURSE, James, C. ,  HAGA, Douwe, D. ,  HARDING, Ian, A. ,  FOCARACCI, Julio, P.

Inventor： YANG, Joon Mo ,  LIU, David, M. ,  CHIANG, Yuh-Min ,  SCHEMBRI, Carol ,  LAU, Aldrich, N. K. ,  ULMANELLA, Umberto ,  BEARD, Nigel, P. ,  SHARIATI, Maryam ,  NURSE, James, C. ,  HAGA, Douwe, D. ,  HARDING, Ian, A. ,  FOCARACCI, Julio, P.

IPC: B01L3/00 ,  C12Q1/68

CPC classification number: B01L3/502707 ,  B01L7/52 ,  B01L2200/141 ,  B01L2300/0816 ,  B01L2300/1805 ,  B01L2300/1861 ,  B01L2400/0677

Abstract: An assay card and devices and methods for isolating chambers on the assay card are described. The assay card comprises a substrate formed of one or more materials, e.g., plastic, having a softening temperature, the substrate defining channels communicating with respective reaction chambers. The assay card may be heated in a region of the channels to at least the softening temperature. The softened plastic may be deformed, e.g., with a tool which may or may not also provide the heat for softening the substrate. In this manner, the plastic of the substrate may be caused to at least partially obstruct the channels, thereby isolating the reaction chambers. The invention also relates to a method of manufacturing a tool device that includes pins for heating and deforming an assay card.

Abstract translation: 描述了用于隔离化验卡上的室的化验卡和装置以及方法。 测定卡包括由具有软化温度的一种或多种材料（例如塑料）形成的基底，基底限定与相应反应室连通的通道。 可以在通道的区域中将测定卡加热到至少软化温度。 软化的塑料可以变形，例如用可以或不可以提供软化基材的热量的工具。 以这种方式，可以使衬底的塑料至少部分地阻塞通道，从而隔离反应室。 本发明还涉及一种制造工具装置的方法，所述工具装置包括用于加热和使检定卡变形的销。

公开(公告)号：WO2008002563A3

公开(公告)日：2008-01-03

申请号：PCT/US2007/014811

申请日：2007-06-26

Applicant: APPLERA CORPORATION ,  HIRANO, Kirk M. ,  BABCOKE, Jason E. ,  CARRILLO, Albert L. ,  HAGA, Douwe D. ,  KAO, Pin ,  KINNEY, Patrick D. ,  NURSE, James C.

Inventor： HIRANO, Kirk M. ,  BABCOKE, Jason E. ,  CARRILLO, Albert L. ,  HAGA, Douwe D. ,  KAO, Pin ,  KINNEY, Patrick D. ,  NURSE, James C.

IPC: C12M1/02

Abstract: A heating apparatus comprising a support base and a microplate having a first surface and an opposing second surface. The microplate is positioned adjacent the support base and comprises a plurality of wells formed in the first surface thereof. Each of the plurality of wells is sized to receive an assay therein. A sapphire crystalline transparent window is positioned adjacent the microplate opposing the support base. A heating device heats the transparent window in response to a control system.

公开(公告)号：WO2006004987A1

公开(公告)日：2006-01-12

申请号：PCT/US2005/023435

申请日：2005-06-29

Applicant: APPLERA CORPORATION ,  HAGA, Douwe, D. ,  FLATBUSH, Michael, R. ,  MOSSEAU, Michael ,  YAN, Tony, S. ,  WIYATNO, Willy ,  REED, Mark, T.

Inventor： HAGA, Douwe, D. ,  FLATBUSH, Michael, R. ,  MOSSEAU, Michael ,  YAN, Tony, S. ,  WIYATNO, Willy ,  REED, Mark, T.

IPC: G01F23/292

CPC classification number: G01F23/292 ,  G01F23/0061 ,  G01F23/2928 ,  G01N9/04 ,  G01N35/028 ,  G01N2035/1025

Abstract: The present application relates to an apparatus and method for measuring liquid levels in small-volume wells, the method comprising : confocally measuring a first distance from a reference point to a top surface of the small-volume well ; confocally measuring a second distance from a reference point to a top surface of the liquid ; and determining a difference between the first distance and the second distance.

Abstract translation: 本申请涉及一种用于测量小体积井中的液面的装置和方法，所述方法包括：共同测量从小体积井的参考点到顶表面的第一距离; 同时测量从参考点到液体顶表面的第二距离; 以及确定所述第一距离和所述第二距离之间的差。

公开(公告)号：WO2009079052A3

公开(公告)日：2009-08-20

申请号：PCT/US2008077092

申请日：2008-09-19

Applicant: APPLIED BIOSYSTEMS ,  YANG JOON MO ,  LIU DAVID M ,  CHIANG YUH-MIN ,  SCHEMBRI CAROL ,  LAU ALDRICH N K ,  ULMANELLA UMBERTO ,  BEARD NIGEL P ,  SHARIATI MARYAM ,  NURSE JAMES C ,  HAGA DOUWE D ,  HARDING IAN A ,  FOCARACCI JULIO P

Inventor： YANG JOON MO ,  LIU DAVID M ,  CHIANG YUH-MIN ,  SCHEMBRI CAROL ,  LAU ALDRICH N K ,  ULMANELLA UMBERTO ,  BEARD NIGEL P ,  SHARIATI MARYAM ,  NURSE JAMES C ,  HAGA DOUWE D ,  HARDING IAN A ,  FOCARACCI JULIO P

IPC: B01L3/00

CPC classification number: B01L3/502707 ,  B01L7/52 ,  B01L2200/141 ,  B01L2300/0816 ,  B01L2300/1805 ,  B01L2300/1861 ,  B01L2400/0677

Abstract: An assay card and devices and methods for isolating chambers on the assay card are described. The assay card comprises a substrate formed of one or more materials, e.g., plastic, having a softening temperature, the substrate defining channels communicating with respective reaction chambers. The assay card may be heated in a region of the channels to at least the softening temperature. The softened plastic may be deformed, e.g., with a tool which may or may not also provide the heat for softening the substrate. In this manner, the plastic of the substrate may be caused to at least partially obstruct the channels, thereby isolating the reaction chambers. The invention also relates to a method of manufacturing a tool device that includes pins for heating and deforming an assay card.

Abstract translation: 描述了用于在测试卡上隔离室的测定卡和装置和方法。 测定卡包括由一种或多种具有软化温度的材料（例如塑料）形成的衬底，衬底限定通道与各个反应室连通。 测定卡可以在通道的区域中被加热到至少软化温度。 软化的塑料可能变形，例如用可能也可能不提供用于软化基底的热量的工具。 以这种方式，可以使衬底的塑料至少部分地阻碍通道，从而隔离反应室。 本发明还涉及一种制造工具装置的方法，该工具装置包括用于加热和变形分析卡的销。

公开(公告)号：WO2004081227A3

公开(公告)日：2004-09-23

申请号：PCT/US2004/007103

申请日：2004-03-09

Applicant: APPLERA CORPORATION ,  OLDHAM, Mark, F. ,  KING, Howard, G. ,  HAGA, Douwe, D. ,  FEREA, Tracy, L.

Inventor： OLDHAM, Mark, F. ,  KING, Howard, G. ,  HAGA, Douwe, D. ,  FEREA, Tracy, L.

IPC: G01N21/64

Abstract: An apparatus for imaging an array of a plurality of features associated with a sample tile. The apparatus includes a stage that supports the sample tile in an illumination region, and an illumination source having a plurality of LEDs adapted to emit light. At least a portion of the light illuminates the illumination region. Additionally, the apparatus includes an image collecting device adapted to selectively collect images of either a first signal when the illumination source is illuminating the illumination region, or a second signal absent illumination of the illumination region. The first signal has wavelengths effectively different from the wavelengths of the portion of the light emitted by the LEDs that illuminates the illumination region.