公开(公告)号：WO2022006588A1

公开(公告)日：2022-01-06

申请号：PCT/US2021/070791

申请日：2021-06-29

申请人： TRUSTEES OF TUFTS COLLEGE ,  BIO-TECHNE CORPORATION

发明人： THEOHARIDES, Theoharis, C. ,  KALABOKIS, Vassilios

IPC分类号： A61K38/20 ,  A61P25/28 ,  C07K14/54

摘要： IL-38 can inhibit activation of microglia and inhibit secretion of IL-Ιβ and CXCL8 from these cells. Analogues of IL-38 and methods of using IL-38 and its analogues to inhibit production of inflammatory mediators in or secretion from cells are provided. Methods of administering these IL-38 analogues to treat neuroinflammatory or neurodegenerative diseases are also provided.

公开(公告)号：WO2019152810A1

公开(公告)日：2019-08-08

申请号：PCT/US2019/016301

申请日：2019-02-01

申请人： BIO-TECHNE CORPORATION

发明人： GOETZ, Christine ,  HAMMERBECK, Christopher ,  VALLEY, Christopher ,  BONNEVIER, Jody ,  AGGELER, Birte ,  WANG, Jinghua ,  WU, Guoping ,  KALABOKIS, Vassilios ,  BAZAN, Jose Fernando

IPC分类号： C07K16/18 ,  G01N33/566

CPC分类号： C07K16/18 ,  C07K2317/92 ,  C07K2319/21 ,  C07K2319/30 ,  C07K2319/33 ,  C07K2319/73 ,  G01N33/566 ,  G01N2500/02 ,  G01N2500/10

摘要： Described herein are polyclonal and monoclonal antibodies that binds to VSIG3, compounds that modulate the interaction of VISTA and VSIG3, and methods of making and using the polyclonal and monoclonal antibodies and compounds. Also described herein are methods that include modulating the interaction of VISTA and VSIG3 by introducing a compound that modulates the interaction of VISTA and VSIG3.

公开(公告)号：WO2022221120A8

公开(公告)日：2022-10-20

申请号：PCT/US2022/023783

申请日：2022-04-07

申请人： BIO-TECHNE CORPORATION ,  RESNIK, Ernesto Rubin ,  BAJER, Anna Agnieszka ,  MUNSHI, Cyrus B. ,  BOSTROM, Kerri Ann ,  BONNEVIER, Jody ,  GOETZ, Christine Andrea

发明人： RESNIK, Ernesto Rubin ,  BAJER, Anna Agnieszka ,  MUNSHI, Cyrus B. ,  BOSTROM, Kerri Ann ,  BONNEVIER, Jody ,  GOETZ, Christine Andrea

IPC分类号： C07K16/10 ,  A61P31/14 ,  A61K39/395 ,  C07K2317/22 ,  C07K2317/33 ,  C07K2317/569 ,  C07K2317/76

摘要： Anti-SARS-CoV-specific llama single domain antibodies, methods of making and characterizing those antibodies, and methods of using those antibodies are described herein. In some embodiments, the antibodies may bind to both SARS-CoV-1 and SARS-CoV-2. In some embodiments, the antibodies bind to S1 of the spike protein of SARS-CoV-2 including, for example, to the receptor binding domain (RBD) of S1. In some embodiments, the antibodies may block the binding of SARS-CoV-1 and/or SARS-CoV-2 to ACE-2.

公开(公告)号：WO2021257716A2

公开(公告)日：2021-12-23

申请号：PCT/US2021/037649

申请日：2021-06-16

申请人： BIO-TECHNE CORPORATION

发明人： JONES, Bryan ,  OTTO, Neil ,  BARNES, Blake

IPC分类号： C12N9/22 ,  C12N15/102 ,  C12N15/11

摘要： The present disclosure provides CRISPR systems using engineered MAD7 endonucleases, as well as methods, vectors, nucleic acid compositions, and kits thereof. In particular, provided herein are MAD7 nickases, catalytically dead MAD7 enzymes, and hyperactive MAD7 enzymes.

公开(公告)号：WO2018027042A1

公开(公告)日：2018-02-08

申请号：PCT/US2017/045314

申请日：2017-08-03

申请人： BIO-TECHNE CORPORATION

发明人： KALABOKIS, Vassilios ,  WANG, Jinghua ,  WU, Guoping ,  BAZAN, Jose, Fernando ,  VALLEY, Christopher, Carlin

IPC分类号： C07K16/28 ,  C07K16/30 ,  A61K39/395

摘要： The ligand for VISTA is identified (VSIG3) as well as the use of this ligand and receptor interaction in the identification or synthesis of a VSIG3 agonist or antagonist compounds, preferably antibodies, polypeptides and fusion proteins which agonize or antagonize the effects of VSIG3 and/or VISTA and/or the VSIG3/VISTA interaction. These antagonists may be used to suppress VSIG/3/VISTA's suppressive effects on T cell immunity, and more particularly used in the treatment of cancer, or infectious disease. These agonist compounds may be used to potentiate or enhance VSIG3/VISTA's suppressive effects on T cell immunity and thereby suppress T cell immunity, such as in the treatment of autoimmunity, allergy or inflammatory conditions. Screening assays for identifying these agonists and antagonist compounds are also provided.

摘要翻译： 识别VISTA的配体（VSIG3）以及该配体和受体相互作用在识别或合成VSIG3激动剂或拮抗剂化合物，优选抗体，多肽和激动剂的融合蛋白质中的用途 或拮抗VSIG3和/或VISTA和/或VSIG3 / VISTA相互作用的影响。 这些拮抗剂可用于抑制VSIG / 3 / VISTA对T细胞免疫的抑制作用，更特别用于治疗癌症或传染病。 这些激动剂化合物可用于增强或增强VSIG3 / VISTA对T细胞免疫的抑制作用，从而抑制T细胞免疫，如用于治疗自身免疫，变态反应或炎症状况。 还提供了用于鉴定这些激动剂和拮抗剂化合物的筛选试验。

公开(公告)号：WO2021087306A1

公开(公告)日：2021-05-06

申请号：PCT/US2020/058282

申请日：2020-10-30

申请人： BIO-TECHNE CORPORATION ,  WU, Zhengliang ,  WHITTAKER, Mark Russell ,  PERSON, Anthony D. ,  KALABOKIS, Vassilios ,  ERTELT, James Michael

发明人： WU, Zhengliang ,  WHITTAKER, Mark Russell ,  PERSON, Anthony D. ,  KALABOKIS, Vassilios ,  ERTELT, James Michael

IPC分类号： G01N33/533 ,  G01N33/58

摘要： This disclosure describes compositions including fluorophore-conj ugated sialic acids and fluorophore-conjugated fucose and methods of making and using those compositions. A fluorophore-conjugated sialic acid may include a cytidine monophosphate activated fluorophore- conjugated sialic acids (CMP-f-SA); a fluorophore-conjugated fucose may include a guanosine 5'-diphosphate activated fluorophore-conjugated fucose (GDP-f-Fuc). Methods of using the compositions include enzymatic incorporation of a fluorophore-conjugated sialic acid or a fluorophore-conjugated fucose or both to label and detect N- and O-glycans on glycoproteins. The compositions and methods may allow for the detection of specific glycans.

公开(公告)号：WO2019074918A1

公开(公告)日：2019-04-18

申请号：PCT/US2018/055001

申请日：2018-10-09

申请人： BIO-TECHNE CORPORATION

发明人： PERSON, Anthony ,  XIONG, Liwen ,  BI, Ming ,  TRACY, Camrin

IPC分类号： A61K38/00 ,  A61K38/02 ,  A61K38/16 ,  A61K38/17 ,  A61K39/00 ,  A61P35/00

摘要： This disclosure describes isolated protein complexes including a Wnt and a sFRP; compositions including a Wnt; a cell overexpressing a Wnt and a sFRP; compositions including a cell overexpressing a Wnt and a cell overexpressing a sFRP; methods of making the protein complexes, compositions, and cells; and methods of using the isolated protein complexes, compositions, and cells. This disclosure further describes methods of forming a complex including a Wnt and a sFRP and methods for isolating a Wnt. Also described herein are methods that may be used to purify a Wnt without the use of a detergent.

公开(公告)号：WO2019005817A3

公开(公告)日：2019-01-03

申请号：PCT/US2018/039532

申请日：2018-06-26

申请人： BIO-TECHNE CORPORATION

发明人： AGGELER, Birte ,  GOETZ, Christine ,  BONNEVIER, Jody ,  VALLEY, Christopher, Carlin ,  HAMMERBECK, Chris ,  RESNIK, Ernesto Ruben

IPC分类号： A61K39/395 ,  A61P35/00 ,  B82Y30/00 ,  C07K14/705 ,  C07K16/28 ,  C12N15/13

摘要： Described herein are monoclonal antibodies that bind to VSIG-4 including antibodies that block the binding of VSIG-4 to SIGLEC-7, compositions including the monoclonal antibodies, and methods of making and using those antibodies and compositions.

公开(公告)号：WO2019005817A2

公开(公告)日：2019-01-03

申请号：PCT/US2018/039532

申请日：2018-06-26

申请人： BIO-TECHNE CORPORATION

发明人： AGGELER, Birte ,  GOETZ, Christine ,  BONNEVIER, Jody ,  VALLEY, Christopher, Carlin ,  HAMMERBECK, Chris

IPC分类号： C07K16/28 ,  A61P35/00

摘要： Described herein are monoclonal antibodies that bind to VSIG-4 including antibodies that block the binding of VSIG-4 to SIGLEC-7, compositions including the monoclonal antibodies, and methods of making and using those antibodies and compositions.

公开(公告)号：WO2018067449A1

公开(公告)日：2018-04-12

申请号：PCT/US2017/054738

申请日：2017-10-02

申请人： BIO-TECHNE CORPORATION

发明人： WU, Zhengliang L. ,  HUANG, Xinyi ,  ETHEN, Cheryl M.

IPC分类号： C12Q1/34 ,  C12Q1/48 ,  G01N33/58

CPC分类号： G01N33/573 ,  C12Q1/34 ,  C12Q1/48 ,  C12Y111/01007 ,  C12Y204/01 ,  C12Y302/01166 ,  G01N33/582 ,  G01N2333/908 ,  G01N2333/91091 ,  G01N2333/91097 ,  G01N2333/924

摘要： A method for assaying endoglycosidase activity includes providing a proteoglycan having a glycosaminoglycan chain with a non-reducing end; treating the proteoglycan with a glycosyltransferase to incorporate a carbohydrate into the non-reducing end of the glycosaminoglycan chain, wherein the carbohydrate includes a click chemistry moiety; adding a label to the proteoglycan, wherein the label includes a click chemistry moiety that reacts to the click chemistry moiety of the carbohydrate such that the label attaches to the carbohydrate to form a labeled proteoglycan; immobilizing the labeled proteoglycan on a multi-well plate, wherein the multi-well plate includes a specific anti-proteoglycan antibody for binding the labeled proteoglycan; treating the labeled proteoglycan with an endoglycosidase specific to the glycosaminoglycan chain; and detecting the labeled proteoglycan.

摘要翻译： 测定内切糖苷酶活性的方法包括提供具有非还原末端的糖胺聚糖链的蛋白多糖; 用糖基转移酶处理蛋白多糖以将碳水化合物掺入糖胺聚糖链的非还原端，其中碳水化合物包括点击化学部分; 向所述蛋白聚糖添加标签，其中所述标签包括点击化学部分，所述点击化学部分与所述碳水化合物的点击化学部分反应，使得所述标记附着于所述碳水化合物以形成标记的蛋白多糖; 将标记的蛋白多糖固定在多孔板上，其中多孔板包含用于结合标记的蛋白多糖的特异性抗蛋白多糖抗体; 用对糖胺聚糖链特异的内切糖苷酶处理标记的蛋白多糖; 并检测标记的蛋白多糖。