公开(公告)号：WO2010107936A2

公开(公告)日：2010-09-23

申请号：PCT/US2010/027690

申请日：2010-03-17

Applicant: BIOMICRO SYSTEMS, INC. ,  EMERY, Dale, B. ,  ADEY, Nils, B.

Inventor： EMERY, Dale, B. ,  ADEY, Nils, B.

IPC: G01N35/10 ,  G01N1/36 ,  G01N1/34

CPC classification number: G01N35/00029 ,  B01L9/52 ,  B01L2200/025 ,  B01L2200/141 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2400/0409 ,  B01L2400/0487 ,  G01N2035/00089 ,  G01N2035/00158

Abstract: A slide-loader system for preparing a microarray slide having a hybridized reaction area for washing, and which includes an immersion tray which contains a volume of wash buffer fluid, and a stripping jig that holds the microarray slide during removal of a reaction chamber housing that is mounted about the hybridized reaction area.

Abstract translation: 一种用于制备具有用于洗涤的杂交反应区域的微阵列载玻片的载玻片装载器系统，其包括含有一定体积的洗涤缓冲液的浸渍托盘和在移除反应室时保持微阵列载玻片的剥离夹具， 围绕杂交反应区安装。

公开(公告)号：WO2003015923A1

公开(公告)日：2003-02-27

申请号：PCT/US2002/025743

申请日：2002-08-13

Applicant: BIOMICRO SYSTEMS, INC. ,  ADEY, Nils ,  LEI, Ming ,  SPUTE, Mark ,  JENSEN, John ,  BEUTEL, Darin ,  SLADE, Devan ,  MCNEELY, Michael

Inventor： ADEY, Nils ,  LEI, Ming ,  SPUTE, Mark ,  JENSEN, John ,  BEUTEL, Darin ,  SLADE, Devan ,  MCNEELY, Michael

IPC: B01L3/02

CPC classification number: B01F11/0071 ,  B01F11/0045 ,  B01F13/0059 ,  B01J19/0093 ,  B01L3/50273 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2400/0481 ,  G01N35/00 ,  G01N2035/00158 ,  G01N2035/00544

Abstract: A method and system for performing mixing in a low volume, low aspect ratio microfluidic chamber (3) is described. Two or more mixing bladders (13,15) formed adjacent the microfluidic chamber are inflated and deflated in reciprocating fashion to cause inward and outward deflection of discrete regions of the chamber wall to mix fluid within the chamber. Mixing bladders are actuated by air or another gas, or by a liquid such as water, pumped in and out of the bladders with a pump which may be located remote from the microfluidic device including the microfluidic chamber. In an alternative embodiment, mixing is generated by applying alternating mechanical forces to a surface of a flexible chamber forming device. The microfluidic chamber may be a hybridization chamber formed on a microarray (25) slide with the use of a microarray interface device, or it may be a microfluidic chamber formed in various other types of microfluidic devices.

Abstract translation: 描述了用于在低体积，低纵横比微流体室（3）中进行混合的方法和系统。 形成在微流体室附近形成的两个或更多个混合气囊（13,15）以往复方式膨胀和放气，以引起室壁离散区域的向内和向外偏转，从而混合室内的流体。 混合气囊由空气或另一种气体或液体如水驱动，用泵泵送进入和离开气囊，泵可以位于远离包括微流体室的微流体装置的位置。 在替代实施例中，通过向柔性室形成装置的表面施加交替的机械力来产生混合。 微流体室可以是使用微阵列界面装置在微阵列（25）载玻片上形成的杂交室，或者它可以是在各种其他类型的微流体装置中形成的微流体室。

公开(公告)号：WO2003052428A1

公开(公告)日：2003-06-26

申请号：PCT/US2002/004045

申请日：2002-02-07

Applicant: BIOMICRO SYSTEMS, INC. ,  MCNEELY, Michael ,  SPUTE, Mark ,  ADEY, Nils

Inventor： SPUTE, Mark ,  ADEY, Nils

IPC: G01N35/08

CPC classification number: B01L3/5027 ,  B01F5/0403 ,  B01F5/064 ,  B01F5/0642 ,  B01F13/0059 ,  B01F15/0232 ,  B01F2015/0221 ,  B01F2215/0037 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00853 ,  B01J2219/00873 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00952 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2200/025 ,  B01L2200/0621 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/165 ,  B01L2300/18 ,  B01L2400/0406 ,  B01L2400/0487 ,  B01L2400/0688 ,  B01L2400/0694 ,  B81B2201/058 ,  B81C3/001 ,  F15C1/14 ,  F15C1/146 ,  F28F2245/04 ,  F28F2260/02 ,  G01N35/08 ,  G01N2035/00237 ,  G01N2035/00366 ,  G01N2035/00544 ,  Y10T137/2076 ,  Y10T137/2224 ,  Y10T137/2267

Abstract: A three-dimensional microfluidic device (100) formed from a plurality of substantially planar layers (101, 102, 103) sealed together is disclosed.

Abstract translation: 公开了由密封在一起的多个基本平坦的层（101,102,103）形成的三维微流体装置（100）。

公开(公告)号：WO2010107936A3

公开(公告)日：2011-01-13

申请号：PCT/US2010027690

申请日：2010-03-17

Applicant: BIOMICRO SYSTEMS INC ,  EMERY DALE B ,  ADEY NILS B

Inventor： EMERY DALE B ,  ADEY NILS B

IPC: G01N35/10 ,  G01N1/34 ,  G01N1/36

CPC classification number: G01N35/00029 ,  B01L9/52 ,  B01L2200/025 ,  B01L2200/141 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2400/0409 ,  B01L2400/0487 ,  G01N2035/00089 ,  G01N2035/00158

Abstract: A slide-loader system for preparing a microarray slide having a hybridized reaction area for washing, and which includes an immersion tray which contains a volume of wash buffer fluid, and a stripping jig that holds the microarray slide during removal of a reaction chamber housing that is mounted about the hybridized reaction area.

Abstract translation: 一种用于制备具有用于洗涤的杂交反应区域的微阵列载玻片的载玻片装载器系统，其包括含有一定体积的洗涤缓冲液的浸渍托盘和在移除反应室时保持微阵列载玻片的剥离夹具， 围绕杂交反应区安装。

公开(公告)号：WO2003098218A1

公开(公告)日：2003-11-27

申请号：PCT/US2002/027347

申请日：2002-08-28

Applicant: BIOMICRO SYSTEMS, INC. ,  LEI, Ming ,  ADEY, Nils ,  MCNEELY, Michael

Inventor： LEI, Ming ,  ADEY, Nils ,  MCNEELY, Michael

IPC: G01N33/558

CPC classification number: B01F5/0403 ,  B01F5/064 ,  B01F5/0642 ,  B01F13/0059 ,  B01F15/0232 ,  B01F2015/0221 ,  B01F2215/0037 ,  B01J19/0093 ,  B01J2219/00837 ,  B01J2219/00891 ,  B01J2219/00995 ,  B01L3/502723 ,  B01L3/502738 ,  B01L3/502746 ,  B01L99/00 ,  B01L2200/025 ,  B01L2200/0621 ,  B01L2200/0684 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0874 ,  B01L2300/0883 ,  B01L2300/0887 ,  B01L2300/165 ,  B01L2300/18 ,  B01L2400/0406 ,  B01L2400/0487 ,  B01L2400/0688 ,  B01L2400/0694 ,  B01L2400/086 ,  F15C1/14 ,  F15C1/146 ,  F28F2210/02 ,  F28F2245/04 ,  F28F2260/02 ,  G01N35/08 ,  G01N35/1004 ,  G01N2035/00237 ,  G01N2035/00366 ,  G01N2035/00544 ,  Y10T137/206 ,  Y10T137/2076 ,  Y10T137/2267

Abstract: Methods and apparatus are presented for controlling fluid flow with pressure gradient fluid control. Passive fluid flow barriers may be used to act as valves, thereby allowing the flow of fluids through flow paths to be regulated so as to allow fluids to be introduced via a single channel (50) and subsequently split into multiple channels (58, 60). Flow through flow paths can be regulated to allow a series of sister wells (51-53) or chambers to all fill prior to the fluid flowing beyond any one of the sister wells (51-53) or chambers. Each flow path may have multiple segments (66-69), at least one of which is designed to balance the pressure drops of the flow paths to provide uniform flow of fluids through the flow paths. The configurations of the wells (51-53) may be modified by adding vents or flow dividers to enhance fluid flushing and gas removal capability.

Abstract translation: 提出了用压力梯度流体控制来控制流体流动的方法和装置。 被动流体阻流板可以用作阀门，从而允许流体流过流动路径被调节，以便允许流体经由单个通道（50）引入并随后分成多个通道（58,60） 。 可以调节通过流路的流动，以允许在流体流过任何一个姐妹井（51-53）或腔室之前，一系列姐妹井（51-53）或腔室全部填充。 每个流动路径可以具有多个段（66-69），其中至少一个被设计成平衡流动路径的压降以提供均匀的流体通过流动路径。 孔（51-53）的结构可以通过添加通风口或分流器来改进，以增强流体冲洗和气体去除能力。

公开(公告)号：WO2003015922A1

公开(公告)日：2003-02-27

申请号：PCT/US2002/024616

申请日：2002-08-02

Applicant: BIOMICRO SYSTEMS, INC. ,  ADEY, Nils

Inventor： ADEY, Nils

IPC: B01L3/02

CPC classification number: G01N35/00 ,  B01F11/0045 ,  B01F13/0059 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00833 ,  B01J2219/00889 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/5085 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2300/0887 ,  G01N33/543 ,  G01N2035/00158 ,  G01N2035/00544

Abstract: A device for forming a low volume reaction chamber (13) on a substrate such as a microarray slide (3) and its method of use is disclosed. The reaction chamber forming device has an adhesive laminate design that permits it to be manufactured as a disposable device. A preferred embodiment of the device includes pneumatically actuatable mixing bladders (23), which can be inflated or deflated to produce deflection of a wall of the reaction chamber in and out with respect to the reaction.

Abstract translation: 公开了一种用于在诸如微阵列载玻片（3）的基板上形成低体积反应室（13）的装置及其使用方法。 反应室形成装置具有允许其被制造为一次性装置的粘合层压板设计。 该装置的优选实施例包括可气动地驱动的混合气囊（23），其可以被充气或放气以产生反应室的壁相对于反应进出的偏转。

公开(公告)号：WO2002072423A1

公开(公告)日：2002-09-19

申请号：PCT/US2002/006942

申请日：2002-03-07

Applicant: BIOMICRO SYSTEMS, INC. ,  MCNEELY, Michael ,  ADEY, Nils

Inventor： MCNEELY, Michael ,  ADEY, Nils

IPC: B65B1/04

CPC classification number: B01L3/502715 ,  B01J2219/00315 ,  B01J2219/00333 ,  B01J2219/00351 ,  B01L3/0203 ,  B01L3/5025 ,  B01L3/50273 ,  B01L3/50853 ,  B01L2200/0642 ,  B01L2300/046 ,  B01L2300/048 ,  B01L2300/049 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0487 ,  C40B60/14

Abstract: A microplate lid (12) containing fluid processing and transport structures is disclosed that provides controlled delivery of small volumes of samples and reagents to a microplate (10), and prevents evaporation during processing.

Abstract translation: 公开了含有流体处理和输送结构的微板盖（12），其提供少量样品和试剂的受控输送到微板（10），并且防止在加工期间的蒸发。

公开(公告)号：WO2002072264A1

公开(公告)日：2002-09-19

申请号：PCT/US2002/007113

申请日：2002-03-08

Applicant: BIOMICRO SYSTEMS, INC. ,  MCNEELY, Michael ,  ADEY, Nils ,  SPUTE, Mark ,  AYLIFFE, Edward ,  HOWARD, Michael ,  BEUTEL, Darin ,  JENSEN, John ,  COFFIN, Stephen ,  MOYER, Thomas

Inventor： MCNEELY, Michael ,  ADEY, Nils ,  SPUTE, Mark ,  AYLIFFE, Edward ,  HOWARD, Michael ,  BEUTEL, Darin ,  JENSEN, John ,  COFFIN, Stephen ,  MOYER, Thomas

IPC: B01L3/02

CPC classification number: B01F5/10 ,  B01F11/0045 ,  B01F11/0266 ,  B01F13/0059 ,  B01J19/0093 ,  B01J2219/00351 ,  B01J2219/0043 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00659 ,  B01L3/0293 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/5085 ,  B01L9/52 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2200/0689 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0877 ,  B01L2300/0883 ,  B01L2300/0887 ,  C40B60/14 ,  G01N33/543 ,  G01N35/00 ,  G01N35/025 ,  G01N35/028 ,  G01N2035/00158 ,  G01N2035/00237 ,  G01N2035/00544

Abstract: A method and system for providing a fluidic interface to slides bearing microarrays of biomolecules or other samples immobilized thereon to perform a variety of chemical reactions or processing steps on the slide. An interface device seals against the slide to form a chamber or chambers containing all or a portion of the microarray, providing selective access to portions of the slide. The interface device includes inlet and outlet ports permitting liquid sample and reagents to be introduced to and removed from the chamber accessing the slide surface. Pre- and post- array microfluidic circuitry may be included in the interface device or in attachable modules. The system may include one or more compartments for collecting and storing waste fluids.

Abstract translation: 一种方法和系统，用于向承载微生物分子的微阵列或固定在其上的其它样品的载玻片提供流体界面，以在载玻片上进行各种化学反应或加工步骤。 接口装置密封滑动件以形成容纳微阵列的全部或一部分的腔室，从而提供对滑块部分的选择性接近。 界面装置包括入口和出口，允许液体样品和试剂被引入和移出进入滑动表面的室。 阵列前和后阵列微流体电路可能包含在接口设备或可连接模块中。 该系统可以包括一个或多个用于收集和储存废液的隔间。