公开(公告)号：WO2010036311A2

公开(公告)日：2010-04-01

申请号：PCT/US2009/005169

申请日：2009-09-16

Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC. ,  MAXHAM, Kenneth, Mark ,  SORENSON, Jon ,  EID, John ,  MARKS, Patrick ,  TRAVERS, Kevin ,  GRAY, Donald ,  EMIG, Robin ,  CHAISSON, Mark ,  FLUSBERG, Benjamin

Inventor： MAXHAM, Kenneth, Mark ,  SORENSON, Jon ,  EID, John ,  MARKS, Patrick ,  TRAVERS, Kevin ,  GRAY, Donald ,  EMIG, Robin ,  CHAISSON, Mark ,  FLUSBERG, Benjamin

IPC: G01N21/00

CPC classification number: C12Q1/6869 ,  G01N2021/7786 ,  Y10T436/143333 ,  C12Q2525/301 ,  C12Q2533/101 ,  C12Q2537/149 ,  C12Q2565/631 ,  C12Q2525/307 ,  C12Q2525/191

Abstract: Methods, devices, and systems for performing intermittent detection during analytical reactions are provided. Such methods facilitate collection of reaction data from disparate reaction times. Further, such methods are useful for reducing photo-induced damage of one or more reactants in an illuminated analytical reaction at a given reaction time. In preferred embodiments, the reaction mixture is subjected to at least one illuminated and non-illuminated period and allowed to proceed such that the time in which the reaction mixture is illuminated is less than a photo-induced damage threshold period.

Abstract translation: 提供了在分析反应期间进行间歇检测的方法，装置和系统。 这样的方法有助于从不同的反应时间收集反应数据。 此外，这样的方法可用于在给定的反应时间下降低照明分析反应中一种或多种反应物的光诱导损伤。 在优选的实施方案中，将反应混合物经历至少一个照明和非照射期，并允许进行，使得反应混合物被照射的时间小于光诱导的损伤阈值期。

公开(公告)号：WO2010144150A2

公开(公告)日：2010-12-16

申请号：PCT/US2010/001690

申请日：2010-06-11

Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC. ,  KORLACH, Jonas ,  TURNER, Stephen ,  FLUSBERG, Benjamin ,  CHAISSON, Mark ,  SCHADT, Eric ,  WEGENER, Jeff

Inventor： KORLACH, Jonas ,  TURNER, Stephen ,  FLUSBERG, Benjamin ,  SCHADT, Eric ,  WEGENER, Jeff

IPC: G01N33/53 ,  G01N33/52 ,  C12Q1/68

CPC classification number: G01N33/68 ,  C12Q1/6825 ,  C12Q1/6827 ,  C12Q1/6869 ,  G01N33/542 ,  G01N33/54306 ,  G01N33/54373 ,  G01N33/582 ,  G01N33/6875 ,  G01N2333/705 ,  G01N2458/10 ,  C12Q2563/107 ,  C12Q2522/101 ,  C12Q2561/113 ,  C12Q2537/164 ,  C12Q2523/125

Abstract: The present invention is generally directed to compositions, methods, and systems for performing single-molecule, real-time analysis of a variety of different biological reactions, and for determining various characteristics of the different biological reactions. The ability to analyze such reactions provides an opportunity to study those reactions as well as to potentially identify factors and/or approaches for impacting such reactions, e.g., to stimulate, enhance, or inhibit such reactions.

Abstract translation: 本发明一般涉及用于进行各种不同生物反应的单分子实时分析以及用于确定不同生物反应的各种特征的组合物，方法和系统。 分析这些反应的能力提供研究这些反应的机会，并且潜在地鉴定影响这些反应的因素和/或方法，例如刺激，增强或抑制这些反应。

公开(公告)号：WO2010144150A3

公开(公告)日：2011-07-14

申请号：PCT/US2010001690

申请日：2010-06-11

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  KORLACH JONAS ,  TURNER STEPHEN ,  FLUSBERG BENJAMIN ,  CHAISSON MARK ,  SCHADT ERIC ,  WEGENER JEFF

Inventor： KORLACH JONAS ,  TURNER STEPHEN ,  FLUSBERG BENJAMIN ,  SCHADT ERIC ,  WEGENER JEFF

IPC: G01N33/53 ,  C12Q1/68 ,  G01N33/52

CPC classification number: G01N33/68 ,  C12Q1/6825 ,  C12Q1/6827 ,  C12Q1/6869 ,  G01N33/542 ,  G01N33/54306 ,  G01N33/54373 ,  G01N33/582 ,  G01N33/6875 ,  G01N2333/705 ,  G01N2458/10 ,  C12Q2563/107 ,  C12Q2522/101 ,  C12Q2561/113 ,  C12Q2537/164 ,  C12Q2523/125

Abstract: The present invention is generally directed to compositions, methods, and systems for performing single-molecule, real-time analysis of a variety of different biological reactions, and for determining various characteristics of the different biological reactions. The ability to analyze such reactions provides an opportunity to study those reactions as well as to potentially identify factors and/or approaches for impacting such reactions, e.g., to stimulate, enhance, or inhibit such reactions.

Abstract translation: 本发明一般涉及用于进行各种不同生物反应的单分子实时分析以及用于确定不同生物反应的各种特征的组合物，方法和系统。 分析这些反应的能力提供研究这些反应的机会，并且潜在地鉴定影响这些反应的因素和/或方法，例如刺激，增强或抑制这些反应。

公开(公告)号：WO2010036311A3

公开(公告)日：2010-06-17

申请号：PCT/US2009005169

申请日：2009-09-16

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  MAXHAM KENNETH MARK ,  SORENSON JON ,  EID JOHN ,  MARKS PATRICK ,  TRAVERS KEVIN ,  GRAY DONALD ,  EMIG ROBIN ,  CHAISSON MARK ,  FLUSBERG BENJAMIN

Inventor： MAXHAM KENNETH MARK ,  SORENSON JON ,  EID JOHN ,  MARKS PATRICK ,  TRAVERS KEVIN ,  GRAY DONALD ,  EMIG ROBIN ,  CHAISSON MARK ,  FLUSBERG BENJAMIN

IPC: G01N21/00

CPC classification number: C12Q1/6869 ,  G01N2021/7786 ,  Y10T436/143333 ,  C12Q2525/301 ,  C12Q2533/101 ,  C12Q2537/149 ,  C12Q2565/631 ,  C12Q2525/307 ,  C12Q2525/191

Abstract: Methods, devices, and systems for performing intermittent detection during analytical reactions are provided. Such methods facilitate collection of reaction data from disparate reaction times. Further, such methods are useful for reducing photo-induced damage of one or more reactants in an illuminated analytical reaction at a given reaction time. In preferred embodiments, the reaction mixture is subjected to at least one illuminated and non-illuminated period and allowed to proceed such that the time in which the reaction mixture is illuminated is less than a photo-induced damage threshold period.

Abstract translation: 提供了用于在分析反应期间执行间歇检测的方法，装置和系统。 这种方法便于从不同的反应时间收集反应数据。 此外，此类方法可用于减少在给定反应时间下照明分析反应中一种或多种反应物的光致损伤。 在优选的实施方案中，使反应混合物经历至少一个照射和未照射的时间段并使其进行，使得照射反应混合物的时间小于光诱导的损伤阈值时间段。