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    MULTI-GENE EXPRESSION VECTORS
    3.
    发明申请
    MULTI-GENE EXPRESSION VECTORS 审中-公开
    多基因表达载体

    公开(公告)号：WO2010002548A2

    公开(公告)日：2010-01-07

    申请号：PCT/US2009046649

    申请日：2009-06-08

    Applicant: EDENSPACE SYSTEMS CORP ,  LEE DAVID A ,  WANG YUEJU

    Inventor： LEE DAVID A ,  WANG YUEJU

    IPC: C12N15/09

    CPC classification number: C12N15/902 ,  C12N15/8201 ,  C12N2799/021

    Abstract: Technologies for stacking multiple genes into a single vector, such as an expression vector. Stacked vectors, cells expressing multiple genes from such vectors, and organisms with stacked traits.

    Abstract translation: 用于将多个基因叠加到单个载体中的技术，如表达载体。 堆叠的载体，表达来自这种载体的多个基因的细胞，以及具有堆叠性状的生物体。

    SYSTEMS TO REDUCE RECALCITRANCE OF CELLULOSIC BIOMASS AND INCREASE YIELDS OF FERMENTABLE SUGARS
    6.
    发明申请
    SYSTEMS TO REDUCE RECALCITRANCE OF CELLULOSIC BIOMASS AND INCREASE YIELDS OF FERMENTABLE SUGARS 审中-公开
    减少细胞生物量回收和增加可食用糖的系统

    公开(公告)号：WO2011160050A3

    公开(公告)日：2012-06-28

    申请号：PCT/US2011040928

    申请日：2011-06-17

    Applicant: EDENSPACE SYSTEMS CORP ,  PAPPAN KIRK ,  BLAYLOCK MICHAEL ,  LEE DAVID ,  FERGUSON BRUCE

    Inventor： PAPPAN KIRK ,  BLAYLOCK MICHAEL ,  LEE DAVID ,  FERGUSON BRUCE

    IPC: A01H5/00 ,  C12N15/55 ,  C12N15/82 ,  C12P19/12

    CPC classification number: C12N9/00 ,  C12N9/18 ,  C12N9/2437 ,  C12N15/8255 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C12Y302/01091

    Abstract: Described herein are methods to reduce the resistance of the plant cell wall to hydrolysis, methods to enhance the conversion of plant biomass into fermentable sugars, methods to increase yields of fermentable sugars from corn grain hydrolysis, and techniques to harvest both the grain and stover from the field simultaneously for hydrolysis. In certain embodiments, the invention provides transgenic plants transformed with expression vectors containing a DNA sequence encoding plant cell wall-modifying enzymes from microbial and animal sources. In certain embodiments, the invention provides methods of improving enzyme performance by creating variants of the parental enzyme. In certain embodiments, the invention provides methods of using stay-green varieties as biomass feedstocks for cellulosic biofuel production. Uses of this invention include, but are not limited to, enhanced biomass conversion, cost-effective lignocellulosic biomass conversion and biofuel production, reduced biomass recalcitrance, enhanced forage digestibility by animals, improved fiber qualities, and enhanced fiber processing properties.

    Abstract translation: 本文描述的是减少植物细胞壁对水解的抗性的方法，增强植物生物质转化为可发酵糖的方法，从玉米粒水解中提高可发酵糖产量的方法，以及收获谷物和秸秆两者的技术 该领域同时进行水解。 在某些实施方案中，本发明提供用含有编码来自微生物和动物来源的植物细胞壁修饰酶的DNA序列的表达载体转化的转基因植物。 在某些实施方案中，本发明提供了通过产生亲本酶的变体来提高酶性能的方法。 在某些实施方案中，本发明提供了使用保留绿色品种作为纤维素生物燃料生产的生物质原料的方法。 本发明的用途包括但不限于增强的生物质转化，成本有效的木质纤维素生物质转化和生物燃料生产，降低的生物质耐受性，动物提高的饲料消化率，改善的纤维品质和增强的纤维加工性能。

    TEMPERING OF CELLULOSIC BIOMASS
    7.
    发明申请
    TEMPERING OF CELLULOSIC BIOMASS 审中-公开
    纤维素生物质的回火

    公开(公告)号：WO2010096510A3

    公开(公告)日：2010-12-16

    申请号：PCT/US2010024505

    申请日：2010-02-17

    Applicant: EDENSPACE SYSTEMS CORP ,  PAPPAN KIRK ,  CORRIDOR DEISY ,  NAIR RAMESH ,  BLAYLOCK MICHAEL ,  CHUMLEY FORREST ,  LEE DAVID ,  FERGUSON BRUCE

    Inventor： PAPPAN KIRK ,  CORRIDOR DEISY ,  NAIR RAMESH ,  BLAYLOCK MICHAEL ,  CHUMLEY FORREST ,  LEE DAVID ,  FERGUSON BRUCE

    IPC: C12N15/82 ,  C12P7/10

    CPC classification number: C12N15/8246 ,  C12N15/8245 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  Y02E50/16

    Abstract: The present invention is directed to improved systems and methods for reducing costs and increasing yields of cellulosic ethanol. In particular, the present invention provides plants genetically transformed for increased biomass, expression of lignocellulolytic enzyme polypeptides, and/or simplification of harvesting and downstream processing. Also provided are methods for processing biomass from these transgenic plants that involve less severe and/or less expensive pre-treatment protocols that are typically employed. Such methods allow, among other things, reduced costs associated with externally applied lignocellulolytic enzyme polypeptides.

    Abstract translation: 本发明涉及用于降低成本和增加纤维素乙醇产量的改进系统和方法。 特别地，本发明提供了遗传转化的植物，用于提高生物量，表达木质纤维素分解酶多肽，和/或简化收获和下游加工。 还提供了用于处理来自这些转基因植物的生物质的方法，其涉及通常使用的较不严格和/或较不昂贵的预处理方案。 除其他方面外，这些方法允许降低与外部应用的木质纤维素分解酶多肽相关的成本。

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