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    NEW BETA-GLUCANASE FROM A BACILLUS
    5.
    发明申请
    NEW BETA-GLUCANASE FROM A BACILLUS 审中-公开
    新的β-葡聚糖酶芽孢

    公开(公告)号：WO99006573A1

    公开(公告)日：1999-02-11

    申请号：PCT/EP1998/004564

    申请日：1998-07-21

    IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/20 ,  C12N1/21 ,  C12N5/10 ,  C12N9/24 ,  C12N9/42 ,  C12N15/56 ,  C12R1/07 ,  C12S9/00 ,  C12P21/02

    CPC分类号： C12Y302/01039 ,  C12N9/2434 ,  C12N9/2448 ,  C12R1/07 ,  C12Y302/01073

    摘要： The aim of the invention is to extend the field of application of beta -glucanases, which are usually used in a slightly acidic to neutral environment, so that they are also stable enough in alkaline conditions to be used in technical processes which are carried out in such conditions. According to the invention, an enzyme obtained from Bacillus alkalophilus DSM 9956, which shows glucanolytic action can be used.

    摘要翻译： β-葡聚糖酶通常是活性在弱酸性至中性范围的应用领域，应该扩展，使得它以在其中发生在碱性介质中的工业过程中使用，甚至在这些条件下具有足够的稳定性。 这是通过一个可得自具有glucanolytic活性嗜碱芽孢杆菌DSM 9956的酶来实现。

    BANANA PROTEINS, DNA, AND DNA REGULATORY ELEMENTS ASSOCIATED WITH FRUIT DEVELOPMENT
    8.
    发明申请
    BANANA PROTEINS, DNA, AND DNA REGULATORY ELEMENTS ASSOCIATED WITH FRUIT DEVELOPMENT 审中-公开
    BANANA蛋白质，DNA和DNA调控元素与水果开发相关

    公开(公告)号：WO99015668A2

    公开(公告)日：1999-04-01

    申请号：PCT/US1998/003343

    申请日：1998-09-23

    IPC分类号： A01H5/00 ,  C07K14/415 ,  C07K14/42 ,  C07K14/825 ,  C12N5/10 ,  C12N9/08 ,  C12N9/10 ,  C12N9/24 ,  C12N15/09 ,  C12N15/29 ,  C12N15/82 ,  C12N15/53 ,  C12N15/54 ,  C12N15/55

    CPC分类号： C12Y302/01073 ,  C07K14/415 ,  C07K14/42 ,  C07K14/825 ,  C12N9/0065 ,  C12N9/1051 ,  C12N9/2442 ,  C12N9/2448 ,  C12N15/8249 ,  C12N15/8257 ,  C12Y302/01014

    摘要： The present invention provides isolated and purified genes which are differentially expressed during banana fruit development, and the protein products of these genes. The present invention further provides DNA regulatory elements which are differentially expressed during banana fruit development, chimeric genes comprising these DNA regulatory elements operably linked to heterologous DNA molecules, and plants transformed with said chimeric genes, providing for controlled expression of said heterologous DNA molecules during the development and ripening of the fruit of said plants, or in response to exogenous ethylene signals in said plants. The present invention also provides a method for expression of a heterologous protein in fruit comprising transforming fruiting plants with one or more chimeric genes according to the present invention, exposing said fruit to an endogenous or exogenous ethylene signal, and harvesting fruit containing said heterologous protein. The method of the present invention may further comprise isolating the proteins produced by said method from the harvested fruit. In a particularly preferred embodiment, the heterologous proteinis a therapeutic protein, which may be isolated from the harvested fruit, or consumed directly in the transformed fruit by a patient in need of said therapeutic protein.

    摘要翻译： 本发明提供了在香蕉果实发育期间差异表达的分离和纯化的基因，以及这些基因的蛋白质产物。 本发明还提供了在香蕉果实发育期间差异表达的DNA调节元件，包含与异源DNA分子可操作地连接的这些DNA调节元件的嵌合基因，以及用所述嵌合基因转化的植物，提供所述异源DNA分子在 所述植物的果实的开发和成熟，或响应于所述植物中的外源乙烯信号。 本发明还提供了一种用于在果实中表达异源蛋白质的方法，包括用根据本发明的一种或多种嵌合基因转化结实植物，将所述果实暴露于内源或外源乙烯信号，以及收获含有所述异源蛋白质的水果。 本发明的方法还可以包括将由所述方法产生的蛋白质与收获的果实分离。 在特别优选的实施方案中，异源蛋白质是治疗性蛋白质，其可以从收获的果实中分离，或由需要所述治疗性蛋白质的患者直接在转化的果实中消耗。

    CHEMICALLY REGULATABLE AND ANTI-PATHOGENIC DNA SEQUENCES AND USES THEREOF
    10.
    发明申请
    CHEMICALLY REGULATABLE AND ANTI-PATHOGENIC DNA SEQUENCES AND USES THEREOF 审中-公开
    化学稳定性和抗病原性DNA序列及其用途

    公开(公告)号：WO1995019443A2

    公开(公告)日：1995-07-20

    申请号：PCT/IB1995000002

    申请日：1995-01-03

    申请人： CIBA-GEIGY AG ,  RYALS, John, A. ,  ALEXANDER, Danny, C. ,  UKNES, Scott, J. ,  WARD, Eric, R.

    发明人： CIBA-GEIGY AG

    IPC分类号： C12N15/82

    CPC分类号： C12N9/2462 ,  C07D285/14 ,  C07K14/415 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/55 ,  C07K2319/61 ,  C12N9/0065 ,  C12N9/0073 ,  C12N9/2442 ,  C12N15/625 ,  C12N15/8238 ,  C12N15/8239 ,  C12N15/8274 ,  C12N15/8282 ,  C12N15/8286 ,  C12Y114/13001 ,  C12Y302/01014 ,  C12Y302/01039 ,  C12Y302/01058 ,  C12Y302/01073 ,  Y02A40/162

    摘要： The present invention provides recombinant or chimaeric DNA molecules comprising plant SAR genes, wherein the wild-type genes corresponding to said SAR genes can be chemically induced in a plant in a protein-synthesis independent or in a protein-synthesis dependent manner. Methods for obtaining these DNA molecules are also provided. Additionally, the present invention provides chemically inducible wheat genes, Arabidopsis chitinase IV, Maize PR-1mz, and Maize thaumatin PR-5mz. The anti-pathogenic sequences according to the invention can be genetically engineered and transformed into plants to obtain transgenic plants with enhanced resistance to disease. In one embodiment of the present invention, the chemically regulatable DNA promoter sequence of the Arabidopsis Pr-1 gene is provided. Another aspect of the present invention is a method of improving protection of a plant against a pest comprising transgenically expressing in said plant two or more DNA molecules encoding anti-pathogenic proteins, wherein the transgenically expressed proteins exert a synergistic effect.

    摘要翻译： 本发明提供了包含植物SAR基因的重组或嵌合DNA分子，其中对应于所述SAR基因的野生型基因可以在蛋白质合成中以蛋白质合成的依赖性方式在植物中化学诱导。 还提供了获得这些DNA分子的方法。 此外，本发明提供化学诱导型小麦基因，拟南芥几丁质酶IV，玉米PR-1mz和玉米丘脑蛋白PR-5mz。 根据本发明的抗病原性序列可以被遗传工程化并转化到植物中以获得具有增强的抗病性的转基因植物。 在本发明的一个实施方案中，提供了拟南芥Pr-1基因的化学上可调节的DNA启动子序列。 本发明的另一方面是改善植物对害虫的保护的方法，包括在所述植物中转基因表达两种或更多种编码抗病原性蛋白质的DNA分子，其中转基因表达的蛋白质发挥协同作用。