公开(公告)号：WO2013177714A1

公开(公告)日：2013-12-05

申请号：PCT/CA2013/050421

申请日：2013-05-31

Applicant: IOGEN ENERGY CORPORATION

Inventor： HILL, Christopher M.D. ,  TOMASHEK, John J. ,  LAVIGNE, James A. ,  MORTIMER, Sandra ,  STORMS, Reginald ,  TSANG, Adrian ,  BUTLER, Greg ,  POWLOWSKI, Justin

IPC: C12N9/42 ,  C12N1/15 ,  C12N15/56 ,  C12N9/24 ,  C12P19/02 ,  C12P19/14 ,  C12P7/10

CPC classification number: C12N9/2437 ,  C12N9/2402 ,  C12N9/2434 ,  C12P19/02 ,  C12P19/14 ,  C12P2203/00 ,  C12Y302/01004 ,  C12Y302/01006 ,  C12Y302/01039 ,  C12Y302/01073 ,  C12Y302/01081 ,  C12Y302/01083 ,  C12Y302/01091 ,  C12Y302/01103 ,  C12Y302/01151 ,  C12Y302/01178 ,  Y02E50/16

Abstract: The present invention relates to a cellulose-degrading enzyme composition comprising one or more cellobiohydrolase and/or endoglucanase enzyme, and an effective amount of an isolated GH16 polypeptide, where the presence of the isolated GH16 polypeptide in the enzyme composition increases the rate or extent of degradation of a cellulosic substrate compared to an equivalent dosage of a corresponding cellulose-degrading enzyme composition lacking the at least one isolated GH16 polypeptide. The present invention also relates to a method for producing fermentable sugars from a cellulosic substrate using the above cellulose- degrading enzyme composition and to genetically modified microbes for produced the above cellulose-degrading enzyme composition.

Abstract translation: 本发明涉及包含一种或多种纤维二糖水解酶和/或内切葡聚糖酶的纤维素降解酶组合物和有效量的分离的GH16多肽，其中酶组合物中分离的GH16多肽的存在增加了 相对于缺乏至少一种分离的GH16多肽的相应的纤维素降解酶组合物的等效剂量，纤维素底物的降解。 本发明还涉及使用上述纤维素分解酶组合物从纤维素底物生产可发酵糖的方法，以及用于生产上述纤维素降解酶组合物的经遗传修饰的微生物。

公开(公告)号：WO2010009515A8

公开(公告)日：2010-04-29

申请号：PCT/AU2009000950

申请日：2009-07-24

Applicant: APPLIMEX SYSTEMS PTY LTD ,  TE O VALENTINO SETOA JUNIOR

Inventor： TE O VALENTINO SETOA JUNIOR

IPC: C12N9/24 ,  C07H3/06 ,  C12N9/14 ,  C12P19/12 ,  C12P39/00

CPC classification number: C12P19/02 ,  C12N9/2402 ,  C12P7/10 ,  C12P19/12 ,  C12P19/14 ,  C12Y301/01072 ,  C12Y302/01004 ,  C12Y302/01008 ,  C12Y302/01021 ,  C12Y302/01037 ,  C12Y302/0105 ,  C12Y302/01055 ,  C12Y302/0108 ,  C12Y302/01091 ,  C12Y302/01113 ,  C12Y302/01151 ,  C12Y302/01155 ,  Y02E50/16

Abstract: The invention relates to methods for the enzymatic hydrolysis of materials. More specifically, the invention relates to a method for hydrolysing a polysaccharide, the method comprising the steps of: (i) contacting the polysaccharide with mesophilic and thermophilic hydrolytic enzymes to produce a reaction mixture, (ii) incubating the reaction mixture at a temperature suitable for activity of the mesophilic hydrolytic enzymes, and (iii) incubating the reaction mixture at a temperature suitable for activity of the thermophilic hydrolytic enzymes, wherein the polysaccharide is hydrolysed by said mesophilic and thermophilic hydrolytic enzymes to produce two or more polysaccharide fragments. The invention further relates to sugar fragments produced by the enzymatic hydrolysis methods.

Abstract translation: 本发明涉及材料的酶水解方法。 更具体地说，本发明涉及一种水解多糖的方法，该方法包括以下步骤：（i）使多糖与嗜温和嗜热水解酶接触以产生反应混合物，（ii）在适合的温度下温育该反应混合物 用于嗜温水解酶的活性，和（iii）在适于嗜热水解酶活性的温度下温育反应混合物，其中多糖被所述嗜温和嗜热水解酶水解以产生两种或更多种多糖片段。 本发明还涉及通过酶水解方法制备的糖片段。

公开(公告)号：WO2009146464A2

公开(公告)日：2009-12-03

申请号：PCT/US2009045883

申请日：2009-06-01

Applicant: EDENSPACE SYSTEMS CORP ,  PAPPAN KIRK ,  NAIR RAMESH ,  CHUMLEY FORREST

Inventor： PAPPAN KIRK ,  NAIR RAMESH ,  CHUMLEY FORREST

IPC: C12N15/55 ,  C12N9/18 ,  C12N9/24 ,  C12N9/42 ,  C12N15/56 ,  C12N15/82

CPC classification number: C12N15/8245 ,  C12N9/18 ,  C12N9/2402 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/248 ,  C12N15/8242 ,  C12N15/8246 ,  C12N15/8255 ,  C12P19/00 ,  C12P19/14 ,  C12Y301/01011 ,  C12Y301/01072 ,  C12Y301/01073 ,  C12Y302/01004 ,  C12Y302/01006 ,  C12Y302/01008 ,  C12Y302/01015 ,  C12Y302/01021 ,  C12Y302/01032 ,  C12Y302/01037 ,  C12Y302/01039 ,  C12Y302/0104 ,  C12Y302/01055 ,  C12Y302/01067 ,  C12Y302/01073 ,  C12Y302/01074 ,  C12Y302/01078 ,  C12Y302/01082 ,  C12Y302/01089 ,  C12Y302/01091 ,  C12Y302/01099 ,  C12Y302/01136 ,  C12Y302/01151 ,  C12Y302/01156 ,  C12Y402/02002 ,  C12Y402/0201 ,  Y02E50/343

Abstract: Improved systems and methods for reducing costs and increasing yields of cellulosic ethanol including compositions of matter comprising plant biomass and cell wall-modifying enzyme polypeptides and transgenic plants expression cell wall- modifying enzyme polypeptides.

Abstract translation: 改进的用于降低成本和提高纤维素乙醇产率的系统和方法，包括包含植物生物量和细胞壁修饰酶多肽的物质组合物以及转基因植物表达细胞壁修饰酶多肽。

公开(公告)号：WO2009033071A3

公开(公告)日：2009-04-30

申请号：PCT/US2008075464

申请日：2008-09-05

Applicant: DYADIC INTERNATIONAL INC ,  MEIJ JACOBA V D ,  EMALFARB MARK ,  GUSAKOV ALEXANDER VASILIEVICH ,  PUNT PETER J ,  SINITSYN ARKADY PANTELEIMONOVI ,  VLASENKO ELENA ,  HINZ SANDRA WIHELMINA AGNES ,  GOSINK MARK ,  JIANG ZHIJIE

Inventor： EMALFARB MARK ,  GUSAKOV ALEXANDER VASILIEVICH ,  PUNT PETER J ,  SINITSYN ARKADY PANTELEIMONOVICH ,  VLASENKO ELENA ,  HINZ SANDRA WIHELMINA AGNES ,  GOSINK MARK ,  JIANG ZHIJIE ,  VERDOES JAN CORNELIS

IPC: C07H21/00 ,  D06L4/40

CPC classification number: C12N9/248 ,  C07H21/00 ,  C12N9/2402 ,  C12N9/2408 ,  C12N9/2434 ,  C12N9/2471 ,  C12Y301/01072 ,  C12Y302/01023 ,  C12Y302/01037 ,  C12Y302/01055 ,  C12Y302/01139 ,  C12Y302/01151 ,  C12Y302/01155 ,  Y02E50/16 ,  Y02E50/17

Abstract: This invention relates to novel enzymes and novel methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material and novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass. The invention also relates to a method to release cellular content by degradation of cell walls. The invention also relates to methods to use the novel enzymes and compositions of such enzymes in a variety of other processes, including washing of clothing, detergent processes, biorefming, deinking and biobleaching of paper and pulp, and treatment of waste streams.

Abstract translation: 本发明涉及新型酶及其制备方法。 更具体地说，本发明涉及各种真菌酶。 描述了编码这些酶的核酸分子，组合物，重组和基因修饰的宿主细胞以及使用方法。 本发明还涉及将木质纤维素生物质转化为可降解木质纤维素材料的酶和可发酵糖的新方法，包括提供植物生物质协同释放糖的那些酶的新组合。 本发明还涉及通过细胞壁降解释放细胞内容物的方法。 本发明还涉及使用这些酶的新型酶和组合物以各种其他方法，包括衣物洗涤，洗涤剂方法，纸和纸浆的生物制备，脱墨和生物漂白以及废物流的处理。

公开(公告)号：WO2009033071A2

公开(公告)日：2009-03-12

申请号：PCT/US2008/075464

申请日：2008-09-05

Applicant: DYADIC INTERNATIONAL, INC. ,  VAN DER MEIJ, Jacoba ,  EMALFARB, Mark ,  GUSAKOV, Alexander, Vasilievich ,  PUNT, Peter, J. ,  SINITSYN, Arkady, Panteleimonovich ,  VLASENKO, Elena ,  HINZ, Sandra Wihelmina, Agnes ,  GOSINK, Mark ,  JIANG, Zhijie

Inventor： EMALFARB, Mark ,  GUSAKOV, Alexander, Vasilievich ,  PUNT, Peter, J. ,  SINITSYN, Arkady, Panteleimonovich ,  VLASENKO, Elena ,  HINZ, Sandra Wihelmina, Agnes ,  GOSINK, Mark ,  JIANG, Zhijie

IPC: C07H21/00

CPC classification number: C12N9/248 ,  C07H21/00 ,  C12N9/2402 ,  C12N9/2408 ,  C12N9/2434 ,  C12N9/2471 ,  C12Y301/01072 ,  C12Y302/01023 ,  C12Y302/01037 ,  C12Y302/01055 ,  C12Y302/01139 ,  C12Y302/01151 ,  C12Y302/01155 ,  Y02E50/16 ,  Y02E50/17

Abstract: This invention relates to novel enzymes and novel methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material and novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass. The invention also relates to a method to release cellular content by degradation of cell walls. The invention also relates to methods to use the novel enzymes and compositions of such enzymes in a variety of other processes, including washing of clothing, detergent processes, biorefming, deinking and biobleaching of paper and pulp, and treatment of waste streams.

Abstract translation: 本发明涉及新型酶及其生产方法。 更具体地，本发明涉及各种真菌酶。 描述了编码这种酶，组合物，重组和遗传修饰的宿主细胞的核酸分子和使用方法。 本发明还涉及将木质纤维素生物质转化为可发酵糖的方法，其中酶可降解木质纤维素材料和酶的新组合，包括提供来自植物生物质的糖的协同释放的酶。 本发明还涉及通过细胞壁降解释放细胞内容物的方法。 本发明还涉及在各种其它方法中使用新型酶和这些酶的组合物的方法，包括洗涤衣物，洗涤剂方法，生物修复，纸和纸浆的脱墨和生物漂白以及废物流的处理。

公开(公告)号：WO2015006634A1

公开(公告)日：2015-01-15

申请号：PCT/US2014/046264

申请日：2014-07-11

Applicant: THE PROCTER & GAMBLE COMPANY

Inventor： FERNANDEZ-PRIETO, Susana ,  LANT, Neil, Joseph ,  GUIDA, Vincenzo

IPC: C11D3/22

CPC classification number: C11D3/222 ,  C11D3/38618 ,  C11D3/38636 ,  C11D3/38645 ,  C11D17/0013 ,  C11D17/0039 ,  C12N9/2411 ,  C12N9/2422 ,  C12N9/2434 ,  C12N9/2437 ,  C12N9/2491 ,  C12N9/48 ,  C12N9/88 ,  C12Y302/01004 ,  C12Y302/01025 ,  C12Y302/01151 ,  C12Y402/02002

Abstract: Microfibrillated cellulose, derived from vegetables or wood, can be used to provide a liquid composition which is compatible with a broad range of ingredients suitable for consumer applications, including enzymes, while still providing good structuring of the liquid composition, without affecting ease of pour.

Abstract translation: 衍生自蔬菜或木材的微纤维素纤维素可用于提供液体组合物，其与广泛范围的适合消费者应用的成分（包括酶）相容，同时仍然提供液体组合物的良好结构，而不影响倾倒性。

公开(公告)号：WO2009146464A3

公开(公告)日：2010-11-18

申请号：PCT/US2009045883

申请日：2009-06-01

Applicant: EDENSPACE SYSTEMS CORP ,  PAPPAN KIRK ,  NAIR RAMESH ,  CHUMLEY FORREST

Inventor： PAPPAN KIRK ,  NAIR RAMESH ,  CHUMLEY FORREST

IPC: A01H5/00 ,  C07K16/00 ,  C12N9/00 ,  C12N15/82 ,  C12Q1/68

CPC classification number: C12N15/8245 ,  C12N9/18 ,  C12N9/2402 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/248 ,  C12N15/8242 ,  C12N15/8246 ,  C12N15/8255 ,  C12P19/00 ,  C12P19/14 ,  C12Y301/01011 ,  C12Y301/01072 ,  C12Y301/01073 ,  C12Y302/01004 ,  C12Y302/01006 ,  C12Y302/01008 ,  C12Y302/01015 ,  C12Y302/01021 ,  C12Y302/01032 ,  C12Y302/01037 ,  C12Y302/01039 ,  C12Y302/0104 ,  C12Y302/01055 ,  C12Y302/01067 ,  C12Y302/01073 ,  C12Y302/01074 ,  C12Y302/01078 ,  C12Y302/01082 ,  C12Y302/01089 ,  C12Y302/01091 ,  C12Y302/01099 ,  C12Y302/01136 ,  C12Y302/01151 ,  C12Y302/01156 ,  C12Y402/02002 ,  C12Y402/0201 ,  Y02E50/343

Abstract: Improved systems and methods for reducing costs and increasing yields of cellulosic ethanol including compositions of matter comprising plant biomass and cell wall-modifying enzyme polypeptides and transgenic plants expression cell wall- modifying enzyme polypeptides.

Abstract translation: 改进的用于降低成本和提高纤维素乙醇产率的系统和方法，包括包含植物生物量和细胞壁修饰酶多肽的物质组合物以及转基因植物表达细胞壁修饰酶多肽。

公开(公告)号：WO2010009515A1

公开(公告)日：2010-01-28

申请号：PCT/AU2009/000950

申请日：2009-07-24

Applicant: APPLIMEX SYSTEMS PTY LTD ,  TE'O, Valentino, Setoa, Junior

Inventor： TE'O, Valentino, Setoa, Junior

IPC: C12N9/24 ,  C12N9/14 ,  C12P39/00 ,  C07H3/06 ,  C12P19/12

CPC classification number: C12P19/02 ,  C12N9/2402 ,  C12P7/10 ,  C12P19/12 ,  C12P19/14 ,  C12Y301/01072 ,  C12Y302/01004 ,  C12Y302/01008 ,  C12Y302/01021 ,  C12Y302/01037 ,  C12Y302/0105 ,  C12Y302/01055 ,  C12Y302/0108 ,  C12Y302/01091 ,  C12Y302/01113 ,  C12Y302/01151 ,  C12Y302/01155 ,  Y02E50/16

Abstract: The invention relates to methods for the enzymatic hydrolysis of materials. More specifically, the invention relates to a method for hydrolysing a polysaccharide, the method comprising the steps of: (i) contacting the polysaccharide with mesophilic and thermophilic hydrolytic enzymes to produce a reaction mixture, (ii) incubating the reaction mixture at a temperature suitable for activity of the mesophilic hydrolytic enzymes, and (iii) incubating the reaction mixture at a temperature suitable for activity of the thermophilic hydrolytic enzymes, wherein the polysaccharide is hydrolysed by said mesophilic and thermophilic hydrolytic enzymes to produce two or more polysaccharide fragments. The invention further relates to sugar fragments produced by the enzymatic hydrolysis methods.

Abstract translation: 本发明涉及用于酶促水解物质的方法。 更具体地说，本发明涉及水解多糖的方法，该方法包括以下步骤：（i）使多糖与嗜温和嗜热水解酶接触以产生反应混合物，（ii）将反应混合物在适合的温度 （iii）将反应混合物在适合于嗜热水解酶活性的温度下孵育，其中所述多糖被所述嗜温和高温水解酶水解以产生两种或更多种多糖片段。 本发明进一步涉及通过酶促水解方法产生的糖片段。

公开(公告)号：WO2009147210A1

公开(公告)日：2009-12-10

申请号：PCT/EP2009/056875

申请日：2009-06-04

Applicant: NOVOZYMES A/S ,  BESENMATTER, Werner ,  FRIIS, Esben Peter ,  GIBSON, Keith ,  RASMUSSEN, Frank Winther ,  SKJOET, Michael

Inventor： BESENMATTER, Werner ,  FRIIS, Esben Peter ,  GIBSON, Keith ,  RASMUSSEN, Frank Winther ,  SKJOET, Michael

IPC: C12N9/42 ,  C12N15/56 ,  C12N1/15 ,  C12N1/21 ,  C11D3/386

CPC classification number: C12N9/2437 ,  C11D3/38636 ,  C12N9/2408 ,  C12N9/2434 ,  C12Y302/01151

Abstract: The present invention relates to variants of a parent xyloglucanase.The present invention also relates to polynucleotides encoding the variant xyloglucanasesand to nucleic acid constructs, vectors, and host cells comprising the polynucleotide.

Abstract translation: 本发明涉及母体木葡聚糖酶的变体。本发明还涉及编码变体木葡聚糖酶的多核苷酸以及包含多核苷酸的核酸构建体，载体和宿主细胞。