公开(公告)号：WO2006116616A3

公开(公告)日：2007-09-27

申请号：PCT/US2006016060

申请日：2006-04-26

Applicant: APPLERA CORP ,  YUE MIN ,  LIU DAVID M ,  CHIANG YUH-MIN ,  YANG JOON MO ,  LEHTO DENNIS ,  VANN CHARLES S ,  BEARD NIGEL P ,  HARDING IAN A ,  VAN CAMP JOHN ,  DROMARETSKY ALEXANDER ,  ERMAKOV SERGEY V ,  OLDHAM MARK F ,  ROY JOY ,  SHARIATI MARYAM ,  ULMANELLA UMBERTO

Inventor： YUE MIN ,  LIU DAVID M ,  CHIANG YUH-MIN ,  YANG JOON MO ,  LEHTO DENNIS ,  VANN CHARLES S ,  BEARD NIGEL P ,  HARDING IAN A ,  VAN CAMP JOHN ,  DROMARETSKY ALEXANDER ,  ERMAKOV SERGEY V ,  OLDHAM MARK F ,  ROY JOY ,  SHARIATI MARYAM ,  ULMANELLA UMBERTO

IPC: C12M1/38

CPC classification number: C12Q1/6806 ,  B01J2219/00317 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/502723 ,  B01L3/50853 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2200/16 ,  B01L2300/041 ,  B01L2300/048 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0655 ,  B01L2400/0694 ,  G01N35/028 ,  Y10T436/2575

Abstract: Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.

Abstract translation: 用于多个分析物检测的系统和方法包括用于分配包括衬底的生物样品的系统，其中所述衬底包括多个样品室，用于每个样品室的样品引入通道和用于每个样品室的排气通道。 该系统可以进一步包括包含在每个样品室中并被配置用于进入基底的生物样品的核酸分析的预加载试剂和被配置为与基底接触以密封每个样品室以便基本上防止 每个样品室中包含的样品从每个样品室流出。 底物可以由检测兼容和测定相容的材料构成。

公开(公告)号：WO2006116616A2

公开(公告)日：2006-11-02

申请号：PCT/US2006/016060

申请日：2006-04-26

Applicant: APPLERA CORPORATION ,  YUE, Min ,  LIU, David M. ,  CHIANG, Yuh-Min ,  YANG, Joon Mo ,  LEHTO, Dennis ,  VANN, Charles S. ,  BEARD, Nigel P. ,  HARDING, Ian A. ,  VAN CAMP, John ,  DROMARETSKY, Alexander ,  ERMAKOV, Sergey V. ,  OLDHAM, Mark F. ,  ROY, Joy ,  SHARIATI, Maryam ,  ULMANELLA, Umberto

Inventor： YUE, Min ,  LIU, David M. ,  CHIANG, Yuh-Min ,  YANG, Joon Mo ,  LEHTO, Dennis ,  VANN, Charles S. ,  BEARD, Nigel P. ,  HARDING, Ian A. ,  VAN CAMP, John ,  DROMARETSKY, Alexander ,  ERMAKOV, Sergey V. ,  OLDHAM, Mark F. ,  ROY, Joy ,  SHARIATI, Maryam ,  ULMANELLA, Umberto

IPC: G01N33/00

CPC classification number: C12Q1/6806 ,  B01J2219/00317 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/502723 ,  B01L3/50853 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2200/16 ,  B01L2300/041 ,  B01L2300/048 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0655 ,  B01L2400/0694 ,  G01N35/028 ,  Y10T436/2575

Abstract: Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.

Abstract translation: 用于多个分析物检测的系统和方法包括用于分配包括衬底的生物样品的系统，其中所述衬底包括多个样品室，用于每个样品室的样品引入通道和用于每个样品室的排气通道。 该系统可以进一步包括包含在每个样品室中并被配置用于进入基底的生物样品的核酸分析的预加载试剂和被配置为与基底接触以密封每个样品室以便基本上防止 每个样品室中包含的样品从每个样品室流出。 底物可以由检测兼容和测定相容的材料构成。

公开(公告)号：WO2009108927A3

公开(公告)日：2009-09-03

申请号：PCT/US2009/035705

申请日：2009-03-02

Applicant: LIFE TECHNOLOGIES CORPORATION ,  BAHATT, Dar ,  ERMAKOV, Sergey, V.

Inventor： BAHATT, Dar ,  ERMAKOV, Sergey, V.

IPC: E02B1/00

Abstract: The present application relates to embodiments of fluidic devices having a pressure-balancing fluidic network designed for positioning an aliquot of liquid in the fluidic network. In various embodiments of a pressure-balancing fluidic device, a fluidic network may be designed so that filing the fluidic network includes loading an aliquot of a fluid through an inlet under an applied pressure, and positioning the aliquot of fluid in the fluidic network using the equalizing forces of the gas pressure in a pressure-compensating portion of the fluidic network and the fluid pressure at the inlet.

公开(公告)号：WO2009108927A2

公开(公告)日：2009-09-03

申请号：PCT/US2009035705

申请日：2009-03-02

Applicant: LIFE TECHNOLOGIES CORP ,  BAHATT DAR ,  ERMAKOV SERGEY V

Inventor： BAHATT DAR ,  ERMAKOV SERGEY V

IPC: B01L3/02

CPC classification number: B01L3/50273 ,  B01L2200/0621 ,  B01L2300/0806 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/14 ,  B01L2400/0487

Abstract: The present application relates to embodiments of fluidic devices having a pressure-balancing fluidic network designed for positioning an aliquot of liquid in the fluidic network. In various embodiments of a pressure-balancing fluidic device, a fluidic network may be designed so that filing the fluidic network includes loading an aliquot of a fluid through an inlet under an applied pressure, and positioning the aliquot of fluid in the fluidic network using the equalizing forces of the gas pressure in a pressure-compensating portion of the fluidic network and the fluid pressure at the inlet.

Abstract translation: 本申请涉及具有设计用于将流体网络中的等分液体定位的压力平衡流体网络的流体装置的实施例。 在压力平衡流体装置的各种实施例中，流体网络可以被设计成使得流体网络的填充包括在施加的压力下加载通过入口的流体的等分试样，并且将流体网络中的等分试样定位在流体网络中 在流体网络的压力补偿部分中的气体压力的平衡力和入口处的流体压力。

公开(公告)号：WO2006076390A1

公开(公告)日：2006-07-20

申请号：PCT/US2006/000890

申请日：2006-01-11

Applicant: APPLERA CORPORATION ,  ERMAKOV, Sergey, V.

Inventor： ERMAKOV, Sergey, V.

IPC: B01J19/00 ,  B01L3/00

CPC classification number: B01L3/50273 ,  B01J19/0046 ,  B01J2219/00367 ,  B01J2219/00369 ,  B01J2219/00389 ,  B01J2219/00439 ,  B01J2219/00441 ,  B01J2219/00448 ,  B01J2219/0045 ,  B01J2219/00675 ,  B01J2219/00695 ,  B01J2219/00722 ,  B01L3/502738 ,  B01L3/502784 ,  B01L3/502792 ,  B01L7/52 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/168 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0427 ,  B01L2400/0448 ,  B01L2400/0487 ,  B01L2400/06 ,  B01L2400/0688 ,  B82Y30/00 ,  C40B40/06 ,  C40B50/14 ,  C40B60/14 ,  F15D1/00 ,  F15D1/06 ,  Y10T137/0391 ,  Y10T137/206 ,  Y10T137/2224 ,  Y10T436/115831 ,  Y10T436/12 ,  Y10T436/2575

Abstract: A fluid processing method, adapted to produce different oligomers in a plurality of respective reaction sites is claimed. The described fluid processing device comprises a first manifold for delivering reactants to the plurality of reaction sites, a second manifold for removing waste from and, optionally, delivering wash fluid to the plurality of reaction sites. Surface tension controlled valves can be disposed in fluid communication with the first manifold, the second manifold or both. They can selectively allow reactants and/or fluids into the reaction sites. The valves can be configured by light activation, e.g. by directing electromagnetic radiation towards them whereby reflecting of the radiation can be controlled by a plurality of mirrors. Electrowetting, optoelectrowetting, thermo capillary effects represent examples of triggering liquid movement. A method of synthesizing oligonucleotides is also disclosed.

Abstract translation: 要求保护适用于在多个相应反应部位产生不同低聚物的流体处理方法。 所描述的流体处理装置包括用于将反应物输送到多个反应部位的第一歧管，用于从多个反应部位除去废物以及任选地将洗涤流体输送到第二歧管的第二歧管。 表面张力控制阀可以设置成与第一歧管，第二歧管或两者流体连通。 它们可以选择性地使反应物和/或流体进入反应位点。 阀可以通过光激活来配置，例如 通过将电磁辐射引向它们，由此可以通过多个反射镜来控制辐射的反射。 电泳，光电移植，热毛细管效应是触发液体运动的示例。 还公开了寡核苷酸的合成方法。