公开(公告)号：WO2011026141A3

公开(公告)日：2011-04-21

申请号：PCT/US2010047402

申请日：2010-08-31

Applicant: LIFE TECHNOLOGIES CORP ,  COX DAVID ,  HABERSTROH MARC ,  KINNEY PATRICK D ,  CARRILLO ALBERT L ,  HOSHIZAKI JON A ,  SHARIATI MARYAM ,  KING HOWARD G ,  LEE JOE Y ,  CHAN MATTHEW S

Inventor： COX DAVID ,  HABERSTROH MARC ,  KINNEY PATRICK D ,  CARRILLO ALBERT L ,  HOSHIZAKI JON A ,  SHARIATI MARYAM ,  KING HOWARD G ,  LEE JOE Y ,  CHAN MATTHEW S

IPC: G02B7/00 ,  G02B21/00

CPC classification number: C12Q1/6869 ,  B01F13/0059 ,  B01F13/0222 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00466 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/16 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2400/0427 ,  C12Q1/6874 ,  G01N35/00069 ,  G01N35/1002 ,  G01N35/1097 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/1034 ,  G02B21/34 ,  Y10T436/11 ,  Y10T436/143333

Abstract: Various embodiments of a sequencing system capable of rapidly imaging samples at multiple wavelengths are provided herein. In one embodiment, the system includes a fast- indexing filter wheel having a plurality of excitation and emission filters capable of being rapidly rotated into and out of communication with an excitation source (e.g., an arc lamp, a laser). For example, the filter wheel can be configured to index in an amount of time falling within a range of about 40 ms to about 60 ms, preferably 50 ms. The system can also be configured to account for vibrations resulting from the quick starts and stops of the fast- indexing filter wheel as well as vibrations resulting from other sources. Various methods of rapidly imaging a sample at multiple wavelength are also provided herein.

Abstract translation: 本文提供了能够快速成像多个波长的样品的测序系统的各种实施例。 在一个实施例中，该系统包括快速分度滤光轮，其具有能够快速旋转进入激发源（例如，弧光灯，激光器）和与激光源（例如，弧光灯，激光器）连通的多个激发和发射滤光片。 例如，滤光轮可被配置成以大约40ms至大约60ms，优选为50ms的范围内的时间量进行索引。 该系统还可以配置为考虑快速启动和停止快速分度滤光轮以及其他来源产生振动的振动。 本文还提供了用于快速成像多个波长的样品的各种方法。

公开(公告)号：WO2011026141A2

公开(公告)日：2011-03-03

申请号：PCT/US2010/047402

申请日：2010-08-31

Applicant: LIFE TECHNOLOGIES CORPORATION ,  COX, David ,  HABERSTROH, Marc ,  KINNEY, Patrick D. ,  CARRILLO, Albert L. ,  HOSHIZAKI, Jon A. ,  SHARIATI, Maryam ,  KING, Howard G. ,  LEE, Joe Y. ,  CHAN, Matthew S.

Inventor： COX, David ,  HABERSTROH, Marc ,  KINNEY, Patrick D. ,  CARRILLO, Albert L. ,  HOSHIZAKI, Jon A. ,  SHARIATI, Maryam ,  KING, Howard G. ,  LEE, Joe Y. ,  CHAN, Matthew S.

IPC: G02B7/00 ,  G02B21/00

CPC classification number: C12Q1/6869 ,  B01F13/0059 ,  B01F13/0222 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00466 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/16 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2400/0427 ,  C12Q1/6874 ,  G01N35/00069 ,  G01N35/1002 ,  G01N35/1097 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/1034 ,  G02B21/34 ,  Y10T436/11 ,  Y10T436/143333

Abstract: Various embodiments of a sequencing system capable of rapidly imaging samples at multiple wavelengths are provided herein. In one embodiment, the system includes a fast-indexing filter wheel having a plurality of excitation and emission filters capable of being rapidly rotated into and out of communication with an excitation source ( e.g. , an arc lamp, a laser). For example, the filter wheel can be configured to index in an amount of time falling within a range of about 40 ms to about 60 ms, preferably 50 ms. The system can also be configured to account for vibrations resulting from the quick starts and stops of the fast-indexing filter wheel as well as vibrations resulting from other sources. Various methods of rapidly imaging a sample at multiple wavelengths are also provided herein.

Abstract translation: 本文提供了能够快速成像多个波长的样品的测序系统的各种实施例。 在一个实施例中，该系统包括具有多个激发和发射滤波器的快速分度滤光轮，该激发和发射滤光片能够快速旋转进入激发源（例如，弧光灯，激光器）和与激发源（例如，弧光灯，激光器）连通。 例如，滤光轮可被配置成以大约40ms至大约60ms，优选为50ms的范围内的时间量进行索引。 该系统还可以被配置为考虑快速启动和停止快速分度滤光轮以及由其他来源产生的振动所产生的振动。 本文还提供了在多个波长下快速成像样品的各种方法。

公开(公告)号：WO2006052682A3

公开(公告)日：2006-05-18

申请号：PCT/US2005/039839

申请日：2005-11-03

Applicant: APPLERA CORPORATION ,  OLDHAM, Mark, F. ,  MIRCHANDANI, Vinod, L. ,  HOSHIZAKI, Jon, A. ,  KING, Howard, G. ,  SLUIS, Johannes, P. ,  BOEGE, Steven, J.

Inventor： OLDHAM, Mark, F. ,  MIRCHANDANI, Vinod, L. ,  HOSHIZAKI, Jon, A. ,  KING, Howard, G. ,  SLUIS, Johannes, P. ,  BOEGE, Steven, J.

IPC: G02B26/10 ,  G01N21/64 ,  G05D23/00 ,  F21K7/00 ,  G01N33/53 ,  H05B33/08

Abstract: The present invention relates to methods and systems for performing sample assays, and for producing and measuring optical responses and signatures. According to various embodyments, a system is provided that includes one or more LED (111), a temperature sensor (118), and a temperature regulator (122). The temperature. sensor can be in thermal contact with the LED (111), can be capable of measuring an operating temperature, and can be capable of generating an operating temperature signal. The temperature regulator can be capable of receiving an operating temperature signal of the LED and regulating the operating temperature based on the operating temperature signal. According to various embodiments, a method for illuminating a reaction region (108) with excitation beams is provided. The method can include providing a system (100) that includes an LED (111) and a reaction region (108). The method can include generating excitation beams with LED; directing the excitation beams toward the reaction region; measuring an operating temperature of the LED; and regulating the operating temperature by transferring heat away from and/or into the LED, based on the measured operating temperature. The reaction region includes a sample.

公开(公告)号：WO2006052682A2

公开(公告)日：2006-05-18

申请号：PCT/US2005039839

申请日：2005-11-03

Applicant: APPLERA CORP ,  OLDHAM MARK F ,  MIRCHANDANI VINOD L ,  HOSHIZAKI JON A ,  KING HOWARD G ,  SLUIS JOHANNES P ,  BOEGE STEVEN J

Inventor： OLDHAM MARK F ,  MIRCHANDANI VINOD L ,  HOSHIZAKI JON A ,  KING HOWARD G ,  SLUIS JOHANNES P ,  BOEGE STEVEN J

IPC: G02B26/10 ,  F21V9/16 ,  G01N21/01 ,  G01N21/64 ,  G01N33/53 ,  G05D23/00 ,  H05B33/08

CPC classification number: G01N21/64 ,  F21K9/00 ,  F21K9/64 ,  F21V29/54 ,  F21V29/677 ,  F21V29/74 ,  F21Y2115/10 ,  G01J3/0286 ,  G01J3/10 ,  G01N21/645 ,  G01N21/6452 ,  G01N2021/6439 ,  G01N2201/06113 ,  G01N2201/062 ,  G01N2201/0638 ,  G01N2201/1211 ,  G05D23/1919 ,  G05D23/20

Abstract: Methods and optical systems for thermally compensated optical scanning of a target sample. The present invention also relates to methods and systems for performing sample assays, and for producing and measuring optical responses and signatures.

Abstract translation: 用于目标样品的热补偿光学扫描的方法和光学系统。 本发明还涉及用于进行样品测定以及用于产生和测量光学响应和特征的方法和系统。