Abstract:
An immobilization unit (820) for the detection of dexoyribonucleic acid (DNA) and ribonucleic acid (RNA) molecules capable of immobilizing nucleic molecules, said immobilization unit comprising a channel of a given depth, width and length and having at least one surface. The surface is adapted to immobilize nucleic molecules under suitable conditions. The channel has at least one straight portion and at least one meandering portion. The at least one meandering portion is essentially u-shaped and the at least one straight portion is an arm of the u-shaped meandering portion.
Abstract:
A device for washing and isolating magnetic particles from a continuous fluid flow in at least one fluidic channel having an inlet at one end and an outlet at another end, said device comprising at least one magnetic source carrier arranged proximate to the at least one fluidic channel. The at least one magnetic source carrier is moveable between a first position and a second position. The at least one magnetic source carrier comprises at least one first magnetic source, wherein a movement of the at least one magnetic source carrier to the first position places the at least one first magnetic source at a first spatial location along the at least one fluidic channel such that said at least one first magnetic source generates a maxima magnetic field at said first spatial location that attracts the magnetic particles from the continuous fluid flow and assembles said magnetic particles at said first spatial location. A movement of the at least one magnetic source carrier to the second position places the at least one first magnetic source distal from the first spatial location such that said magnetic field at said first spatial location is at a minima and the magnetic particles disperse from said first spatial location.
Abstract:
An immobilization unit (820) for the detection of dexoyribonucleic acid (DNA) and ribonucleic acid (RNA) molecules capable of immobilizing nucleic molecules, said immobilization unit comprising a channel of a given depth, width and length and having at least one surface. The surface is adapted to immobilize nucleic molecules under suitable conditions. The channel has at least one straight portion and at least one meandering portion. The at least one meandering portion is essentially u-shaped and the at least one straight portion is an arm of the u-shaped meandering portion.
Abstract:
Microfluidics packages and methods of use are described, comprising in one embodiment a substrate having a top surface and means to lower pressure on the top surface; a fluidics card having a bottom surface and means to allow fluids to traverse through the card; and a polymeric barrier film, the polymeric barrier film positioned between the top surface of the substrate and the bottom surface of the fluidics card.
Abstract:
Microfluidics mixing apparatus and methods of using same are disclosed for mixing fluids using increasing centrifugal force as the fluids being mixed traverse a mixing channel. One inventive apparatus comprises a generally planar substrate having a top major surface and a bottom major surface generally parallel to the top major surface, and a cover plate over the top major surface. The substrate has at least one inlet port that routes fluid to the top major surface, and at least one outlet port for mixed fluid. The substrate comprises a mixing channel having a depth measured from the top surface and a width, the mixing channel adapted to route fluids to be mixed therein in laminar flow and in a substantially spiral flow pattern that is parallel to the top surface. Apparatus of the invention can mix fluids flowing serially, or two or more fluids entering the device from different feed channels.
Abstract:
A device for washing and isolating magnetic particles from a continuous fluid flow in at least one fluidic channel having an inlet at one end and an outlet at another end, said device comprising at least one magnetic source carrier arranged proximate to the at least one fluidic channel. The at least one magnetic source carrier is moveable between a first position and a second position. The at least one magnetic source carrier comprises at least one first magnetic source, wherein a movement of the at least one magnetic source carrier to the first position places the at least one first magnetic source at a first spatial location along the at least one fluidic channel such that said at least one first magnetic source generates a maxima magnetic field at said first spatial location that attracts the magnetic particles from the continuous fluid flow and assembles said magnetic particles at said first spatial location. A movement of the at least one magnetic source carrier to the second position places the at least one first magnetic source distal from the first spatial location such that said magnetic field at said first spatial location is at a minima and the magnetic particles disperse from said first spatial location.
Abstract:
A microfluidics package (1) comprising a substrate (6) having a top surface, said top surface comprises at least one fluid channel (11, 12), at least one fluidic chip (2) having a top surface, a bottom surface, at least one side surface, and at least one passage to allow a fluid to traverse from the top surface or any side surface to the bottom surface of the chip; and a sheet (4) of which both sides are adhesive, wherein the first adhesive side of the sheet (4) is secured to the substrate (6), and the at least one fluidic chip (2) is secured by the second adhesive side of the sheet (4), said fluidic chip (2) being arranged such that the at least one passage of the fluidic chip is in fluid communication with the at least one fluid channel (11, 12) of the substrate.
Abstract:
Microfluidics chips and methods of use are described, comprising a pair of wafers (102, 108), at least one having a patterned surface (108), and two polymeric barrier films (104, 106) between the wafers conforming to the patterned surface. The polymeric barrier films allow the wafers of the inventive microfluidics chips to be reused without cleaning.