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    NUCLEIC ACID ENCODING REACTIONS
    2.
    发明申请
    NUCLEIC ACID ENCODING REACTIONS 审中-公开
    核酸编码反应

    公开(公告)号：WO2012162267A2

    公开(公告)日：2012-11-29

    申请号：PCT/US2012/038894

    申请日：2012-05-21

    Applicant: FLUIDIGM CORPORATION ,  ANDERSON, Megan ,  CHEN, Peilin ,  FOWLER, Brian ,  JONES, Robert C. ,  KAPER, Fiona ,  LEBOFSKY, Ronald ,  MAY, Andrew

    Inventor： ANDERSON, Megan ,  CHEN, Peilin ,  FOWLER, Brian ,  JONES, Robert C. ,  KAPER, Fiona ,  LEBOFSKY, Ronald ,  MAY, Andrew

    IPC: C40B20/00 ,  C40B50/06 ,  C40B40/06 ,  C40B30/04 ,  C12P19/34 ,  C12Q1/68

    CPC classification number: C12Q1/6855 ,  C12N15/10 ,  C12N15/1065 ,  C12N15/65 ,  C12N15/66 ,  C12Q1/686 ,  C12Q1/6874

    Abstract: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.

    Abstract translation: 本文所描述的方法可用于掺入一个或多个衔接子和/或核苷酸标签和/或条形码核苷酸序列，一个或多个靶核苷酸序列。 在具体实施方案中，产生具有适配器（例如适合用于高通量DNA测序）的核酸片段。 在其它实施方案中，关于反应混合物的信息被编码成反应产物。 本文还描述了可用于扩增一种或多种靶核酸以准备应用如双向核酸测序的方法和试剂盒。 在具体实施方案中，本发明的方法还需要进行双向DNA测序。 本文还描述了通过引物延伸来编码和检测和/或定量等位基因的方法。

    MULTI-PRIMER AMPLIFICATION METHOD FOR BARCODING OF TARGET NUCLEIC ACIDS
    3.
    发明申请
    MULTI-PRIMER AMPLIFICATION METHOD FOR BARCODING OF TARGET NUCLEIC ACIDS 审中-公开
    用于靶向靶核酸的多重PCR扩增方法

    公开(公告)号：WO2010115154A1

    公开(公告)日：2010-10-07

    申请号：PCT/US2010/029854

    申请日：2010-04-02

    Applicant: FLUIDIGM CORPORATION ,  MAY, Andrew ,  CHEN, Peilin ,  WANG, Jun ,  KAPER, Fiona ,  ANDERSON, Megan

    Inventor： MAY, Andrew ,  CHEN, Peilin ,  WANG, Jun ,  KAPER, Fiona ,  ANDERSON, Megan

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/52 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0655 ,  C12Q1/686 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2527/143 ,  C12Q2535/122 ,  C12Q2549/119 ,  C12Q2563/179 ,  C12Q2565/629

    Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

    Abstract translation: 在某些实施方案中，本发明提供了其中将核苷酸标签和任选的条形码核苷酸序列加入靶核苷酸序列的扩增方法。 在其它实施例中，本发明提供一种包括多个第一输入线和多个第二输入线的微流体装置。 微流体装置还包括多组第一腔室和多组第二腔室。 每组第一腔室与多个第一输入管线中的一个流体连通。 每组第二腔室与多个第二输入管线中的一个流体连通。 微流体装置还包括与多个第二输入线的第一部分流体连通的多个第一泵元件和与多个第二输入线的第二部分流体连通的多个第二泵元件。

    SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS
    4.
    发明申请
    SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS 审中-公开
    颗粒群体的单粒子分析

    公开(公告)号：WO2013177206A3

    公开(公告)日：2014-01-30

    申请号：PCT/US2013042086

    申请日：2013-05-21

    Applicant: FLUIDIGM CORP ,  ANDERSON MEGAN ,  CHEN PEILIN ,  FOWLER BRIAN ,  KAPER FIONA ,  LEBOFSKY RONALD ,  MAY ANDREW

    Inventor： ANDERSON MEGAN ,  CHEN PEILIN ,  FOWLER BRIAN ,  KAPER FIONA ,  LEBOFSKY RONALD ,  MAY ANDREW

    IPC: C12Q1/68

    CPC classification number: C12Q1/6813 ,  B01D15/08 ,  C12Q1/6806 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

    Abstract: In certain embodiments, the invention provides methods and devices for assaying single particles in a population of particles, wherein at least two parameters are measured for each particle. One or more parameters can be measured while the particles are in the separate reaction volumes. Alternatively or in addition, one or more parameters can be measured in a later analytic step, e.g., where reactions are carried out in the separate reaction volumes and the reaction products are recovered and analyzed. In particular embodiments, one or more parameter measurements are carried out "in parallel," i.e., essentially simultaneously in the separate reaction volumes.

    Abstract translation: 在某些实施方案中，本发明提供了用于测定颗粒群体中的单个颗粒的方法和装置，其中针对每个颗粒测量至少两个参数。 当颗粒处于分开的反应体积中时，可以测量一个或多个参数。 或者或另外，可以在稍后的分析步骤中测量一个或多个参数，例如其中在分开的反应体积中进行反应并回收和分析反应产物。 在具体实施方案中，一个或多个参数测量“并行地进行”，即基本上同时在单独的反应体积中进行。

    SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS
    5.
    发明申请
    SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS 审中-公开
    颗粒群体的单粒子分析

    公开(公告)号：WO2013177206A2

    公开(公告)日：2013-11-28

    申请号：PCT/US2013/042086

    申请日：2013-05-21

    Applicant: FLUIDIGM CORPORATION ,  ANDERSON, Megan ,  CHEN, Peilin ,  FOWLER, Brian ,  KAPER, Fiona ,  LEBOFSKY, Ronald ,  MAY, Andrew

    Inventor： ANDERSON, Megan ,  CHEN, Peilin ,  FOWLER, Brian ,  KAPER, Fiona ,  LEBOFSKY, Ronald ,  MAY, Andrew

    IPC: C12Q1/68

    CPC classification number: C12Q1/6813 ,  B01D15/08 ,  C12Q1/6806 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

    Abstract: In certain embodiments, the invention provides methods and devices for assaying single particles in a population of particles, wherein at least two parameters are measured for each particle. One or more parameters can be measured while the particles are in the separate reaction volumes. Alternatively or in addition, one or more parameters can be measured in a later analytic step, e.g., where reactions are carried out in the separate reaction volumes and the reaction products are recovered and analyzed. In particular embodiments, one or more parameter measurements are carried out "in parallel," i.e., essentially simultaneously in the separate reaction volumes.

    Abstract translation: 在某些实施方案中，本发明提供用于测定颗粒群体中的单个颗粒的方法和装置，其中针对每个颗粒测量至少两个参数。 当颗粒处于分开的反应体积中时，可以测量一个或多个参数。 或者或另外，可以在稍后的分析步骤中测量一个或多个参数，例如其中在分开的反应体积中进行反应并回收和分析反应产物。 在具体实施方案中，“并行地”执行一个或多个参数测量，即基本上同时在单独的反应体积中进行。

    NUCLEIC ACID ENCODING REACTIONS
    6.
    发明申请
    NUCLEIC ACID ENCODING REACTIONS 审中-公开
    核酸编码反应

    公开(公告)号：WO2012162267A3

    公开(公告)日：2014-05-15

    申请号：PCT/US2012038894

    申请日：2012-05-21

    Applicant: FLUIDIGM CORP ,  ANDERSON MEGAN ,  CHEN PEILIN ,  FOWLER BRIAN ,  JONES ROBERT C ,  KAPER FIONA ,  LEBOFSKY RONALD ,  MAY ANDREW

    Inventor： ANDERSON MEGAN ,  CHEN PEILIN ,  FOWLER BRIAN ,  JONES ROBERT C ,  KAPER FIONA ,  LEBOFSKY RONALD ,  MAY ANDREW

    IPC: C12Q1/68 ,  C12P19/34

    CPC classification number: C12Q1/6855 ,  C12N15/10 ,  C12N15/1065 ,  C12N15/65 ,  C12N15/66 ,  C12Q1/686 ,  C12Q1/6874

    Abstract: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.

    Abstract translation: 本文所描述的方法可用于掺入一个或多个衔接子和/或核苷酸标签和/或条形码核苷酸序列，一个或多个靶核苷酸序列。 在具体实施方案中，产生具有适配器（例如适合用于高通量DNA测序）的核酸片段。 在其它实施方案中，关于反应混合物的信息被编码成反应产物。 本文还描述了可用于扩增一种或多种靶核酸以准备应用如双向核酸测序的方法和试剂盒。 在具体实施方案中，本发明的方法还需要进行双向DNA测序。 本文还描述了通过引物延伸来编码和检测和/或定量等位基因的方法。

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