公开(公告)号：WO2010117420A3

公开(公告)日：2011-03-24

申请号：PCT/US2010000948

申请日：2010-03-29

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  KONG XIANGXU ,  SHEN GENE

Inventor： KONG XIANGXU ,  SHEN GENE

IPC: G01N33/52 ,  C12Q1/68

CPC classification number: C12Q1/6818 ,  C07H19/207 ,  C12Q1/6869 ,  G01N21/6428 ,  G01N33/542 ,  G01N33/582 ,  G01N2021/6441 ,  Y10T436/143333

Abstract: FRET-labeled compounds are provided for use in analytical reactions. In certain embodiments, FRET-labeled nucleotide analogs are used in place of naturally occurring nucleoside triphosphates or other analogs in analytical reactions comprising nucleic acids, for example, template-directed nucleic acid synthesis, DNA sequencing, RNA sequencing, single-base identification, hybridization, binding assays, and other analytical reactions.

Abstract translation: 提供FRET标记的化合物用于分析反应。 在某些实施方案中，FRET标记的核苷酸类似物用于代替包含核酸的分析反应中天然存在的核苷三磷酸或其它类似物，例如模板指导的核酸合成，DNA测序，RNA测序，单碱基鉴定，杂交 ，结合测定和其他分析反应。

公开(公告)号：WO2010117420A2

公开(公告)日：2010-10-14

申请号：PCT/US2010/000948

申请日：2010-03-29

Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC. ,  KONG, Xiangxu ,  SHEN, Gene

Inventor： KONG, Xiangxu ,  SHEN, Gene

IPC: G01N33/52 ,  C12Q1/68

CPC classification number: C12Q1/6818 ,  C07H19/207 ,  C12Q1/6869 ,  G01N21/6428 ,  G01N33/542 ,  G01N33/582 ,  G01N2021/6441 ,  Y10T436/143333

Abstract: FRET-labeled compounds are provided for use in analytical reactions. In certain embodiments, FRET-labeled nucleotide analogs are used in place of naturally occurring nucleoside triphosphates or other analogs in analytical reactions comprising nucleic acids, for example, template-directed nucleic acid synthesis, DNA sequencing, RNA sequencing, single-base identification, hybridization, binding assays, and other analytical reactions.

Abstract translation: 提供FRET标记的化合物用于分析反应。 在某些实施方案中，FRET标记的核苷酸类似物用于代替包含核酸的分析反应中天然存在的核苷三磷酸或其它类似物，例如模板指导的核酸合成，DNA测序，RNA测序，单碱基鉴定，杂交 ，结合测定和其他分析反应。

公开(公告)号：WO2010059880A3

公开(公告)日：2010-05-27

申请号：PCT/US2009/065222

申请日：2009-11-19

Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC. ,  KONG, Xiangxu ,  SHEN, Gene ,  FEDEROV, Andrei ,  LYLE, John ,  LEE, Grace ,  SEBO, Lubomir ,  DO, Duc ,  WEBER, Robert ,  DUDEK, Steve

Inventor： KONG, Xiangxu ,  SHEN, Gene ,  FEDEROV, Andrei ,  LYLE, John ,  LEE, Grace ,  SEBO, Lubomir ,  DO, Duc ,  WEBER, Robert ,  DUDEK, Steve

IPC: G01N33/52 ,  C12Q1/48 ,  C07C50/04

Abstract: Compositions, devices, systems and methods for reducing and/or preventing photo-induced damage of one or more reactants in an illuminated analytical reaction by addition of one or more photo-induced damage mitigating agents to the reaction mixture and allowing the reaction to proceed for a period that is less than a photo-induced damage threshold period.

公开(公告)号：WO2009100382A1

公开(公告)日：2009-08-13

申请号：PCT/US2009/033467

申请日：2009-02-06

Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC. ,  OTTO, Geoffrey ,  SHEN, Gene ,  KONG, Xiangxu ,  EMIG, Robin

Inventor： OTTO, Geoffrey ,  SHEN, Gene ,  KONG, Xiangxu ,  EMIG, Robin

IPC: C07H21/00

CPC classification number: C12Q1/48 ,  C07H21/00 ,  C12Q1/68 ,  C12Q1/686

Abstract: The present invention provides methods and compositions for performing illuminated reactions, particularly sequencing reactions, while mitigating and/or preventing photodamage to reactants that can result from prolonged illumination. In particular, the invention provides methods and compositions for incorporating photoprotective agents into conjugates comprising reporter molecules and nucleoside polyphosphates.

Abstract translation: 本发明提供用于进行照明反应，特别是测序反应的方法和组合物，同时减轻和/或防止可能由长时间照射引起的反应物的光损伤。 特别地，本发明提供了将光保护剂掺入包含报道分子和核苷多磷酸盐的缀合物中的方法和组合物。

公开(公告)号：WO2012021733A3

公开(公告)日：2012-08-02

申请号：PCT/US2011047468

申请日：2011-08-11

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  YUE STEPHEN ,  WEBER ROBERT ,  KONG XIANGXU ,  FEDOROV ANDREI ,  LYLE JOHN

Inventor： YUE STEPHEN ,  WEBER ROBERT ,  KONG XIANGXU ,  FEDOROV ANDREI ,  LYLE JOHN

IPC: G01N33/52 ,  C12Q1/25 ,  C12Q1/68

CPC classification number: C12Q1/6869 ,  C12N13/00 ,  C12Q1/25 ,  G01N33/52 ,  G01N33/54373 ,  G01N2333/91245 ,  G01N2333/916

Abstract: Compositions, devices, systems and methods for reducing and/or preventing photo-induced damage of one or more reactants in an illuminated analytical reaction by addition of one or more photoprotective compounds to the reaction mixture and allowing the reaction to proceed for a period that is less than a photo-induced damage threshold period.

Abstract translation: 用于通过向反应混合物中加入一种或多种光保护性化合物并允许反应进行一段时间，以减少和/或防止照明分析反应中的一种或多种反应物的光诱导损伤的组合物，装置，系统和方法 小于光致伤害阈值期。

公开(公告)号：WO2012021733A2

公开(公告)日：2012-02-16

申请号：PCT/US2011/047468

申请日：2011-08-11

Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC. ,  YUE, Stephen ,  WEBER, Robert ,  KONG, Xiangxu ,  FEDOROV, Andrei ,  LYLE, John

Inventor： YUE, Stephen ,  WEBER, Robert ,  KONG, Xiangxu ,  FEDOROV, Andrei ,  LYLE, John

IPC: G01N33/52 ,  C12Q1/25 ,  C12Q1/68

CPC classification number: C12Q1/6869 ,  C12N13/00 ,  C12Q1/25 ,  G01N33/52 ,  G01N33/54373 ,  G01N2333/91245 ,  G01N2333/916

Abstract: Compositions, devices, systems and methods for reducing and/or preventing photo-induced damage of one or more reactants in an illuminated analytical reaction by addition of one or more photoprotective compounds to the reaction mixture and allowing the reaction to proceed for a period that is less than a photo-induced damage threshold period.

Abstract translation: 用于通过向反应混合物添加一种或多种光保护性化合物来减少和/或防止照射分析反应中一种或多种反应物的光致损伤的组合物，装置，系统和方法， 反应进行的时间小于光诱导损伤阈值时间。

公开(公告)号：WO2010059880A2

公开(公告)日：2010-05-27

申请号：PCT/US2009065222

申请日：2009-11-19

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  KONG XIANGXU ,  SHEN GENE ,  FEDEROV ANDREI ,  LYLE JOHN ,  LEE GRACE ,  SEBO LUBOMIR ,  DO DUC ,  WEBER ROBERT ,  DUDEK STEVE

Inventor： KONG XIANGXU ,  SHEN GENE ,  FEDEROV ANDREI ,  LYLE JOHN ,  LEE GRACE ,  SEBO LUBOMIR ,  DO DUC ,  WEBER ROBERT ,  DUDEK STEVE

IPC: G01N33/52 ,  C07C50/04 ,  C12Q1/48

CPC classification number: C07C49/255 ,  G01N21/6428 ,  G01N33/5306 ,  G01N2021/6432

Abstract: Compositions, devices, systems and methods for reducing and/or preventing photo-induced damage of one or more reactants in an illuminated analytical reaction by addition of one or more photo-induced damage mitigating agents to the reaction mixture and allowing the reaction to proceed for a period that is less than a photo-induced damage threshold period.

Abstract translation: 用于通过向反应混合物添加一种或多种光诱导损伤缓解剂并允许反应进行以减少和/或防止照射分析反应中一种或多种反应物的光诱导损伤的组合物，装置，系统和方法 小于光诱导损伤阈值时间的时间段。