公开(公告)号：WO2009145828A2

公开(公告)日：2009-12-03

申请号：PCT/US2009002003

申请日：2009-03-30

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  BJORNSON KEITH ,  LEE HAROLD ,  KORLACH JONAS ,  TURNER STEPHEN ,  BIBILLO AREK ,  CHRISTIANS FRED ,  EMIG ROBIN ,  HE MOLLY ,  PARK INSIL ,  JIA LEI ,  FEDOROV ANDREI ,  LYLE JOHN

Inventor： BJORNSON KEITH ,  LEE HAROLD ,  KORLACH JONAS ,  TURNER STEPHEN ,  BIBILLO AREK ,  CHRISTIANS FRED ,  EMIG ROBIN ,  HE MOLLY ,  PARK INSIL ,  JIA LEI ,  FEDOROV ANDREI ,  LYLE JOHN

IPC: C12Q1/68 ,  C12N9/12 ,  C12Q1/48

CPC classification number: C12Q1/6869 ,  C12Q1/6874 ,  C12Q2527/125 ,  C12Q2527/119 ,  C12Q2527/101 ,  C12Q2565/1015 ,  C12Q2527/137 ,  C12Q2521/101

Abstract: Compositions, kits, methods and systems for nucleotide sequencing comprising producing polymerase reactions that exhibit two kinetically observable steps within an observable phase of the polymerase reaction. Two slow step systems can be produced, for example, by selecting the appropriate polymerase enzyme, polymerase reaction conditions including cofactors, and polymerase reaction substrates including the primed template and nucleotides.

Abstract translation: 用于核苷酸测序的组合物，试剂盒，方法和系统，其包括产生聚合酶反应，其在聚合酶反应的可观察期内显示两个动力学可观察步骤。 可以通过例如选择合适的聚合酶，包括辅因子的聚合酶反应条件和包括引发的模板和核苷酸的聚合酶反应底物来产生两个缓慢的步骤系统。

公开(公告)号：WO2009089056A1

公开(公告)日：2009-07-16

申请号：PCT/US2009/000146

申请日：2009-01-09

Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC. ,  LUONG, Khai ,  LUNDQUIST, Paul ,  DALAL, Ravi ,  LYLE, John ,  TURNER, Stephen

Inventor： LUONG, Khai ,  LUNDQUIST, Paul ,  DALAL, Ravi ,  LYLE, John ,  TURNER, Stephen

IPC: G01N21/64 ,  G01N21/00

CPC classification number: C12M1/34 ,  G01N21/6428 ,  G01N21/645 ,  G01N2021/6417 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2021/7786 ,  Y10T436/2525

Abstract: Methods, systems and their components for monitoring fluorescent signals and particularly transient fluorescent signals from reaction mixtures of interest, which methods and systems employ modulated excitation light sources to reduce impacts of excessive illumination on the reaction components or the data obtained therefrom.

Abstract translation: 用于监测来自感兴趣的反应混合物的荧光信号和特别是瞬时荧光信号的方法，系统及其组件，该方法和系统采用调制的激发光源以减少过度照明对反应组分的影响或从其获得的数据。

公开(公告)号：WO2007076057A3

公开(公告)日：2008-06-26

申请号：PCT/US2006049122

申请日：2006-12-21

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  HANZEL DAVID K ,  OTTO GEOFF ,  MURPHY DEVON ,  PELUSO PAUL ,  PHAM THANG ,  RANK DAVID R ,  MITSIS PAUL ,  CHRISTIANS FRED C ,  BIBILLO AREK ,  PARK INSIL ,  CLARK SONYA ,  LYLE JOHN

Inventor： HANZEL DAVID K ,  OTTO GEOFF ,  MURPHY DEVON ,  PELUSO PAUL ,  PHAM THANG ,  RANK DAVID R ,  MITSIS PAUL ,  CHRISTIANS FRED C ,  BIBILLO AREK ,  PARK INSIL ,  CLARK SONYA ,  LYLE JOHN

IPC: C12Q1/68

CPC classification number: C12N9/1252 ,  C07K2319/20 ,  C12P19/34 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Y207/07007 ,  Y02P20/52

Abstract: Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.

Abstract translation: 提供了包含具有改善核苷酸类似物进入活性位点区域并与活性位点区域中的核苷酸类似物配位的特征的聚合酶的组合物。 还提供了制备聚合酶和在测序和DNA复制和扩增中使用聚合酶的方法以及聚合酶活性的动力学模型和使用该模型的计算机实现的方法。

公开(公告)号：WO2007081941B1

公开(公告)日：2008-01-10

申请号：PCT/US2007000514

申请日：2007-01-09

Applicant: LYLE CORPORATE DEV ,  LYLE JOHN W ,  LYLE BRENTON ,  ELLSWORTH ROBERT S

Inventor： LYLE JOHN W ,  LYLE BRENTON ,  ELLSWORTH ROBERT S

IPC: A63F9/24

CPC classification number: A63F13/79 ,  A63F13/12 ,  A63F13/65 ,  A63F13/822 ,  A63F2300/5506 ,  A63F2300/5546 ,  A63F2300/5553 ,  A63F2300/695

Abstract: A video game character or avatar is generated using images of the player so that the on-screen avatar being controlled by the player appears like the player. Answers to a questionnaire or a psychological profile are provided by the player to determine characteristics that the player views as desirable, which are used to generate a final version of the avatar. Iterations of the avatar appearance are generated from the initial appearance to the final, more desirable appearance. Using the avatar, as play of the game proceeds the player's avatar begins as a character appearing like themselves and gradually becomes a character that is more as they would like to appear. The avatar increases the emotional impact of games by providing strong visual and psychological connections with the player. The avatar may instead begin from an initial character that does not appear similar to the player. The iterations may make the avatar gradually less appealing or with other changes in appearance, in some embodiments.

Abstract translation: 使用播放器的图像生成视频游戏角色或头像，使得由玩家控制的屏幕上的头像看起来像播放器。 玩家提供问卷或心理资料的答案，以确定玩家视为期望的特征，用于生成化身的最终版本。 化身外观的迭代从最初的外观生成到最终的，更理想的外观。 使用头像，随着游戏的进行，玩家的头像开始为像自己一样的角色，并逐渐变成更像他们想要出现的角色。 头像通过与玩家提供强烈的视觉和心理联系来增加游戏的情感影响。 头像可以改为从与玩家不相似的初始角色开始。 在一些实施例中，迭代可以使化身逐渐变得不吸引人或外观上的其他改变。

公开(公告)号：WO1989002896A1

公开(公告)日：1989-04-06

申请号：PCT/US1988003361

申请日：1988-10-05

Applicant: ML TECHNOLOGY VENTURES, L.P. ,  ARNOLD, Lyle, John ,  NELSON, Norman, Charles

Inventor： ML TECHNOLOGY VENTURES, L.P.

IPC: C07H21/02

CPC classification number: C07H21/00

Abstract: Methods for the construction, labelling and subsequent purification of nucleic acid probes containing primary amines with acridinium esters 4-(2-succinimidyloxycarbonyl-ethyl)phenyl-10-methylacridinium-9-carboxylate fluorosulfonate). The method for attaching acridinium esters to probes uses high (0.1 to 50mM) acridinium ester concentrations achieved using organic solvent in concentrations of 20 % to 80 % by volume, and may be carried out either in solution, or with one or the other of the acridinium ester or the probe suspended in solution. Purification (the separation of labelled probe from unlabelled probe and free label) involves (1) first removing most of the free acridinium ester label from probe using rapid separation techniques, then (2) removing substantially all remaining free label from the probe and separating labelled probe from unlabelled probe, involves specific applications of ion exchange, reverse phase or hydroxyapatite HPLC.

公开(公告)号：WO2012021733A3

公开(公告)日：2012-08-02

申请号：PCT/US2011047468

申请日：2011-08-11

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  YUE STEPHEN ,  WEBER ROBERT ,  KONG XIANGXU ,  FEDOROV ANDREI ,  LYLE JOHN

Inventor： YUE STEPHEN ,  WEBER ROBERT ,  KONG XIANGXU ,  FEDOROV ANDREI ,  LYLE JOHN

IPC: G01N33/52 ,  C12Q1/25 ,  C12Q1/68

CPC classification number: C12Q1/6869 ,  C12N13/00 ,  C12Q1/25 ,  G01N33/52 ,  G01N33/54373 ,  G01N2333/91245 ,  G01N2333/916

Abstract: Compositions, devices, systems and methods for reducing and/or preventing photo-induced damage of one or more reactants in an illuminated analytical reaction by addition of one or more photoprotective compounds to the reaction mixture and allowing the reaction to proceed for a period that is less than a photo-induced damage threshold period.

Abstract translation: 用于通过向反应混合物中加入一种或多种光保护性化合物并允许反应进行一段时间，以减少和/或防止照明分析反应中的一种或多种反应物的光诱导损伤的组合物，装置，系统和方法 小于光致伤害阈值期。

公开(公告)号：WO2012021733A2

公开(公告)日：2012-02-16

申请号：PCT/US2011/047468

申请日：2011-08-11

Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC. ,  YUE, Stephen ,  WEBER, Robert ,  KONG, Xiangxu ,  FEDOROV, Andrei ,  LYLE, John

Inventor： YUE, Stephen ,  WEBER, Robert ,  KONG, Xiangxu ,  FEDOROV, Andrei ,  LYLE, John

IPC: G01N33/52 ,  C12Q1/25 ,  C12Q1/68

CPC classification number: C12Q1/6869 ,  C12N13/00 ,  C12Q1/25 ,  G01N33/52 ,  G01N33/54373 ,  G01N2333/91245 ,  G01N2333/916

Abstract: Compositions, devices, systems and methods for reducing and/or preventing photo-induced damage of one or more reactants in an illuminated analytical reaction by addition of one or more photoprotective compounds to the reaction mixture and allowing the reaction to proceed for a period that is less than a photo-induced damage threshold period.

Abstract translation: 用于通过向反应混合物添加一种或多种光保护性化合物来减少和/或防止照射分析反应中一种或多种反应物的光致损伤的组合物，装置，系统和方法， 反应进行的时间小于光诱导损伤阈值时间。

公开(公告)号：WO2010059880A2

公开(公告)日：2010-05-27

申请号：PCT/US2009065222

申请日：2009-11-19

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  KONG XIANGXU ,  SHEN GENE ,  FEDEROV ANDREI ,  LYLE JOHN ,  LEE GRACE ,  SEBO LUBOMIR ,  DO DUC ,  WEBER ROBERT ,  DUDEK STEVE

Inventor： KONG XIANGXU ,  SHEN GENE ,  FEDEROV ANDREI ,  LYLE JOHN ,  LEE GRACE ,  SEBO LUBOMIR ,  DO DUC ,  WEBER ROBERT ,  DUDEK STEVE

IPC: G01N33/52 ,  C07C50/04 ,  C12Q1/48

CPC classification number: C07C49/255 ,  G01N21/6428 ,  G01N33/5306 ,  G01N2021/6432

Abstract: Compositions, devices, systems and methods for reducing and/or preventing photo-induced damage of one or more reactants in an illuminated analytical reaction by addition of one or more photo-induced damage mitigating agents to the reaction mixture and allowing the reaction to proceed for a period that is less than a photo-induced damage threshold period.

Abstract translation: 用于通过向反应混合物添加一种或多种光诱导损伤缓解剂并允许反应进行以减少和/或防止照射分析反应中一种或多种反应物的光诱导损伤的组合物，装置，系统和方法 小于光诱导损伤阈值时间的时间段。

公开(公告)号：WO2007123763A3

公开(公告)日：2008-11-06

申请号：PCT/US2007008019

申请日：2007-03-29

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  RANK DAVID R ,  WEGENER JEFFERY ,  KORLACH JONAS ,  ROITMAN DANIEL ,  XU YUE ,  LYLE JOHN ,  TURNER STEPHEN ,  PELUSO PAUL ,  OTTO GEOFF

Inventor： RANK DAVID R ,  WEGENER JEFFERY ,  KORLACH JONAS ,  ROITMAN DANIEL ,  XU YUE ,  LYLE JOHN ,  TURNER STEPHEN ,  PELUSO PAUL ,  OTTO GEOFF

IPC: C12N11/00 ,  C40B40/04

CPC classification number: C12Q1/6874 ,  B01J19/0046 ,  B01J2219/00427 ,  B01J2219/0045 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00639 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B82Y20/00 ,  B82Y30/00 ,  C12Q1/6837 ,  Y10T29/49002 ,  C12Q2565/629 ,  C12Q2527/127

Abstract: Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.

Abstract translation: 通过使用底物的元素来辅助活性化学基团在基底的所需区域中的定位来制备具有选定的活性化学区域的基底的方法。 所述方法可以包括用于沉积，去除，激活和/或去活衬底的选定区域中的化学基团的光学，化学和/或机械方法，以提供衬底的选择性活性区域。

公开(公告)号：WO2007070067A1

公开(公告)日：2007-06-21

申请号：PCT/US2006/002145

申请日：2006-01-20

Applicant: LYLE CORPORATE DEVELOPMENT, INC. ,  LYLE, John

Inventor： LYLE, John

IPC: A61K31/565 ,  A61K31/566 ,  A61P17/00

CPC classification number: A61K31/565 ,  A61K31/566

Abstract: Methods and formulations for the regeneration of vaginal tissue and vaginal cell health resulting from vaginal cell hypoxia in a human female. A pharmaceutical composition for topical non-systemic administration is formulated containing a hormonal agent administered to the vagina, vulvar area of the individual undergoing treatment.

Abstract translation: 用于阴道组织再生和由阴道细胞缺氧导致的阴道细胞健康的方法和制剂。 配制用于局部非全身给药的药物组合物，其含有施用于经历治疗的个体的阴道外阴区域的激素剂。