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    PRODUCTION OF A LIPID ACYLTRANSFERASE FROM TRANSFORMED BACILLUS LICHENIFORMIS CELLS
    4.
    发明申请
    PRODUCTION OF A LIPID ACYLTRANSFERASE FROM TRANSFORMED BACILLUS LICHENIFORMIS CELLS 审中-公开
    从转化的BACILLUS LICHENIFORMIS细胞中产生脂质过氧化物酶

    公开(公告)号：WO2008090395A1

    公开(公告)日：2008-07-31

    申请号：PCT/IB2007/000558

    申请日：2007-01-25

    Applicant: DANISCO A/S ,  KOLKMAN, Marc ,  MIKKELSEN, Jørn, Dalgaard ,  LORENTSEN, Rikke, Høegh

    Inventor： KOLKMAN, Marc ,  MIKKELSEN, Jørn, Dalgaard ,  LORENTSEN, Rikke, Høegh

    IPC: C12N9/18 ,  C12N15/75 ,  C12N1/21

    CPC classification number: C12N9/18 ,  C12N9/1029 ,  C12N15/75

    Abstract: The present invention relates to a method for the production of a lipid acyltransferase comprising the steps of: (i) providing a Bacillus licheniformis cell; (ii) transforming the Bacillus licheniformis cell with a heterologous nucleotide sequence encoding a lipid acyltransferase; and (iii) expressing the lipid acyltransferase in the cell under the control of a promoter sequence. In addition, the present invention further relates to the use of Bacillus licheniformis to express a lipid acyltransferase, a Bacillus licheniformis host cell comprising a heterologous lipid acyltransferase and a vector comprising a nucleotide sequence encoding a lipid acyltransferase operably linked to a promoter sequence homologous to B. licheniformis .

    Abstract translation: 本发明涉及一种生产脂质酰基转移酶的方法，包括以下步骤：（i）提供地衣芽孢杆菌细胞; （ii）用编码脂质酰基转移酶的异源核苷酸序列转化地衣芽孢杆菌细胞; 和（iii）在启动子序列控制下的细胞中表达脂质酰基转移酶。 此外，本发明还涉及地衣芽孢杆菌表达脂质酰基转移酶，包含异源脂质酰基转移酶的地衣芽孢杆菌宿主细胞的使用和包含编码脂质酰基转移酶的核苷酸序列的载体，所述核苷酸序列可操作地连接到与B同源的启动子序列 地衣芽孢杆菌

    FUNGAL LIPOLYTIC ENZYMES
    5.
    发明申请
    FUNGAL LIPOLYTIC ENZYMES 审中-公开

    公开(公告)号：WO2005087918A3

    公开(公告)日：2005-09-22

    申请号：PCT/IB2005/000875

    申请日：2005-03-10

    Applicant: DANISCO A/S ,  BRUNSTEDT, Janne ,  MIKKELSEN, Jørn, Dalgaard ,  PEDERSEN, Henrik ,  SØE, Jørn, Borch

    Inventor： BRUNSTEDT, Janne ,  MIKKELSEN, Jørn, Dalgaard ,  PEDERSEN, Henrik ,  SØE, Jørn, Borch

    IPC: C12N9/00

    Abstract: A fungal wild-type lipolytic enzyme having a higher ratio of activity on polar lipids compared with triglycerides, wherein the enzyme preferably has a phospholipidAriglyceride hydrolysing activity ratio of at least 4. Preferably, the lipolytic enzyme according to the present invention has a glycolipid:triglyceride hydrolysing activity ratio of at least 1.5. In one embodiment, the fungal lipolytic enzyme according to the present invention comprises an amino acid sequence as shown in SEQ ID No. 1 or SEQ ID No. 2 or SEQ ID No. 4 or SEQ ID No. 6 or an amino acid sequence which has at least 90% identity thereto. The present invention further encompasses a nucleic acid encoding a fungal lipolytic enzyme, which nucleic acid is selected from the group consisting of: (a) a nucleic acid comprising a nucleotide sequence shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7; (b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7 by the degeneration of the genetic code; and (c) nucleic acid comprising a nucleotide sequence which has at least 90% identity with the nucleotide sequence shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7.

    ENZYMATIC TREATMENT OF OILS
    6.
    发明申请
    ENZYMATIC TREATMENT OF OILS 审中-公开

    公开(公告)号：WO2005066351A3

    公开(公告)日：2005-07-21

    申请号：PCT/IB2004/004374

    申请日：2004-12-23

    Applicant: DANISCO A/S ,  KRISTENSEN, Anna, Cecilie, Jentoft ,  WASSELL, Paul ,  MIKKELSEN, Jørn, Dalgaard ,  SØE, Jørn, Borch

    Inventor： KRISTENSEN, Anna, Cecilie, Jentoft ,  WASSELL, Paul ,  MIKKELSEN, Jørn, Dalgaard ,  SØE, Jørn, Borch

    IPC: C12P7/64

    Abstract: The present invention relates to a method of reducing and/or removing diglyceride from an edible oil, comprising a) admixing an edible oil with an acyl acceptor substrate and a fatty-acid CoA independent diglyceride: glycerol acyltransferase, wherein the fatty-acid CoA independent diglyceride: glycerol acyltransferase is characterized as an enzyme which in an edible oil is capable of transferring an acyl group from a diglyceride to glycerol. Preferably, the diglyceride: glycerol acyltransferase comprises the amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, H, Q, T, N, M or S. Furthermore the present invention relates to the use of a fatty-acid CoA independent diglyceride: glycerol acyltransferase characterized as an enzyme which in an edible oil is capable of transferring an acyl group from a diglyceride to glycerol, in the manufacture of an edible oil, for reducing and/or removing (preferably selectively reducing and/or removing) diglyceride from said edible oil, and to the use of said enzyme in the manufacture of a foodstuff comprising an edible oil for improving the crystallization properties of said foodstuff.

    LIPOLYTIC ENZYME USES THEREOF IN THE FOOD INDUSTRY
    9.
    发明申请
    LIPOLYTIC ENZYME USES THEREOF IN THE FOOD INDUSTRY 审中-公开
    蛋白

    公开(公告)号：WO2006008653A2

    公开(公告)日：2006-01-26

    申请号：PCT/IB2005/002602

    申请日：2005-07-18

    Applicant: DANISCO A/S ,  MIASNIKOV, Andrei ,  SØE, Jørn, Borch ,  MIKKELSEN, Jørn, Dalgaard ,  POVELAINEN, Mira ,  PITKANEN, Virve

    Inventor： MIASNIKOV, Andrei ,  SØE, Jørn, Borch ,  MIKKELSEN, Jørn, Dalgaard ,  POVELAINEN, Mira ,  PITKANEN, Virve

    IPC: C11B3/00 ,  C11C3/00 ,  C07F9/10 ,  C12N15/55 ,  C12N9/20 ,  C12P7/64 ,  A23C9/13 ,  A21D2/26

    CPC classification number: C12N9/1029 ,  A21D8/042 ,  A23J7/00 ,  C11B3/003 ,  C12N9/20 ,  C12P7/6418 ,  C12P7/6445 ,  C12P7/6481 ,  C12Y301/01003

    Abstract: The invention also encompases the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein said lipolytic enzyme is capable of hydrolysing a glycolipid or a phospholipid or transferring an acyl group from a glycolipid or phospholipids to a acyl acceptor. Preferably, the lipolytic enzyme for use in these methods and uses comprises an amino acid sequence as shown in any one of SEQ. ID. No.s 4, 5, 7, 8, 12, 14 or 16 or an amino acid sequence having at least 70% identity therewith or comprises a nucleotide sequence shown as SEQ ID No. 3, 6, 9, 13, 15 or 17 or a nulceotide seqeunce which has at least 70% identity therewith. The present invention also relates to a lipolytic enzyme capable of hydrolysing at least a galactolipid or capable of transferring an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Steptomyces species and includes a lipolytic enzyme comprising an amino acid sequence as shown in SEQ ID No. 4 or an amino acid sequence which has at least 60% identity thereto. The enzyme may be encoded by a nucleic acid selected from the group consisting of: a) a nucleic acid comprising a nucleotide sequence shown in SEQ ID No. 3; b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3 by the degeneration of the genetic code; and c) a nucleic acid comprising a nucleotide sequence which has at least 70% identity with the nucleotide sequence shown in SEQ ID No. 3.

    Abstract translation: 本发明还包括使用可从各种方法获得的脂肪分解酶：链霉菌，棒状杆菌和热嗜热菌，其中所述脂肪分解酶能够水解糖脂或磷脂或从糖脂转移酰基 或磷脂转化为酰基受体。 优选地，用于这些方法和用途的脂肪分解酶包含SEQ ID NO：1中任一项所示的氨基酸序列。 ID。 第4,5,7,8,12,14或16号氨基酸序列或与其具有至少70％同一性的氨基酸序列，或包含SEQ ID No.3,6,13,13,15或17所示的核苷酸序列 或与其具有至少70％同一性的nucceotide seqonunce。 本发明还涉及能够至少水解半乳糖脂或能够将酰基从半乳糖脂转移至一种或多种酰基受体底物的脂解酶，其中所述酶可从步态物种获得，并且包括脂肪分解酶，其包含氨基 如SEQ ID No.4所示的酸序列或与其具有至少60％同一性的氨基酸序列。 该酶可以由选自以下的核酸编码：a）包含SEQ ID No.3所示核苷酸序列的核酸; b）通过遗传密码的退化与SEQ ID No.3的核苷酸序列相关的核酸; 和c）包含与SEQ ID No.3所示核苷酸序列具有至少70％同一性的核苷酸序列的核酸。

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