公开(公告)号：WO2006014351A2

公开(公告)日：2006-02-09

申请号：PCT/US2005023520

申请日：2005-07-01

Applicant: BLUESHIFT BIOTECHNOLOGIES INC ,  COMITA PAUL B ,  SHUMATE CHRISTOPHER B ,  MILLER STEVEN C ,  CROMWELL EVAN F

Inventor： COMITA PAUL B ,  SHUMATE CHRISTOPHER B ,  MILLER STEVEN C ,  CROMWELL EVAN F

IPC: C12Q1/70 ,  C12Q1/68 ,  G01N33/53

CPC classification number: G01N33/582 ,  B82Y5/00 ,  B82Y10/00 ,  C12Q1/6816 ,  G01N21/6408 ,  G01N21/6428 ,  G01N21/6445 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6432 ,  G01N2021/6441 ,  G01N2021/6478 ,  G01N2021/6484 ,  G01N2201/067 ,  G01N2201/0697 ,  G01N2201/0833 ,  C12Q2563/107 ,  C12Q2561/119

Abstract: Methods, apparatus, and system, implementing and using techniques for detecting a presence of one or more target analytes in particular regions of interest of one or more samples. One or more samples including objects and one or more target analytes are provided. Some of the target analytes are labeled with a fluorophore and are bound to some of the objects in the samples. The samples are illuminated with fluorescence inducing light and fluorescent light is collected from one or more regions of the one or more samples. At least one anisotropy measurement of the samples is performed to identify regions of interest where one or more target analytes are bound to the objects. The collected fluorescent light from the regions of interest is analyzed to determine a presence of target analytes that are bound to the objects in the one or more samples.

Abstract translation: 方法，装置和系统，实施和使用用于检测一个或多个样品的特定感兴趣区域中一种或多种目标分析物的存在的技术。 提供一个或多个样品，包括物体和一种或多种目标分析物。 一些目标分析物用荧光团标记，并且与样品中的某些对象结合。 用荧光诱导光照射样品，并从一个或多个样品的一个或多个区域采集荧光。 进行样品的至少一个各向异性测量以识别一个或多个目标分析物与物体结合的感兴趣区域。 分析来自感兴趣区域的收集的荧光以确定与一个或多个样品中的物体结合的目标分析物的存在。

公开(公告)号：WO2006107864A1

公开(公告)日：2006-10-12

申请号：PCT/US2006/012292

申请日：2006-04-03

Applicant: BLUESHIFT BIOTECHNOLOGIES, INC. ,  MILLER, Steven, C. ,  COMITA, Paul, B. ,  SHUMATE, Christopher, B. ,  CROMWELL, Evan, F.

Inventor： MILLER, Steven, C. ,  COMITA, Paul, B. ,  SHUMATE, Christopher, B. ,  CROMWELL, Evan, F.

IPC: C12Q1/68 ,  G01N33/53

CPC classification number: G01N33/542 ,  G01N2500/00

Abstract: Methods and apparatus are described for detecting specific binding between first and second chemical entities. The first chemical entity in association with a first fluorophore is immobilized. The second chemical entity is allowed to bind with the immobilized first chemical entity. The second chemical entity is or becomes coupled to a second fluorophore, which forms a FRET pair with the first fluorophore. The bound chemical entities are exposed to radiation at an excitation frequency for either the first or the second fluorophore, and polarization anisotropy of a FRET fluorescent signal from the bound chemical entities is measured to detect specific binding between the first and second chemical entities. Techniques are also disclosed for detecting whether a FRET interaction is occurring between a first chemical entity including a donor fluorophore and a second chemical entity including an acceptor fluorophore, using simultaneous anisotropy measurements at the wavelengths of the donor and acceptor fluorophores.

Abstract translation: 描述了用于检测第一和第二化学实体之间的特异性结合的方法和装置。 与第一荧光团相关联的第一化学实体被固定化。 允许第二化学实体与固定化的第一化学实体结合。 第二化学实体是或连接到与第一荧光团形成FRET对的第二荧光团。 结合的化学实体以第一或第二荧光团的激发频率暴露于辐射，并且测量来自结合的化学实体的FRET荧光信号的偏振各向异性以检测第一和第二化学实体之间的特异性结合。 还公开了用于检测在包括供体荧光团的第一化学实体和包括受体荧光团的第二化学实体之间是否发生FRET相互作用的技术，其使用在供体和受体荧光团的波长处的同时各向异性测量。

公开(公告)号：WO2005081801A3

公开(公告)日：2005-11-10

申请号：PCT/US2005004055

申请日：2005-02-09

Applicant: BLUESHIFT BIOTECHNOLOGIES INC ,  CROMWELL EVAN F ,  MILLER STEVEN C ,  SHUMATE CHRISTOPHER B ,  COMITA PAUL B

Inventor： CROMWELL EVAN F ,  MILLER STEVEN C ,  SHUMATE CHRISTOPHER B ,  COMITA PAUL B

IPC: B01L3/00 ,  G01N1/10 ,  G01N21/25 ,  G01N21/64 ,  G01N35/02

CPC classification number: G01N21/253 ,  B01L3/5085 ,  B01L2300/0806 ,  G01N21/6408 ,  G01N21/6445 ,  G01N21/6452 ,  G01N35/028

Abstract: Methods and apparatus for assaying biological materials employ multi-well substrates as described herein. The substrates (102) include a plurality of wells (201) typically each of several nanoliters volume or smaller having consistent dimensions and formed in a rigid substrate (102) s a glass disk. Each well (201) provided with a circumferential lip to minimize crosstalk between wells and/or facilitate optical location of the individual wells during interrogation. Samples are provided to the individual wells and assayed by an optical technique (119) employing fluorescence, polarization, reflectance, or the like. A scanning laser system (111) may be employed for this purpose. The substrate may rotate during the scan to allow consistent interrogation of the wells without stopping and starting the rotation. Multiple rotations may also be employed repeatedly interrogate the samples for use in a kinetic study, for example.

Abstract translation: 用于测定生物材料的方法和装置采用本文所述的多孔底物。 衬底（102）包括通常具有一定尺寸的几个纳升体积或更小的多个孔（201），并形成在刚性基底（102）玻璃盘中。 每个井（201）设置有圆周唇缘，以在询问期间最小化井之间的串扰和/或促进各个井的光学定位。 将样品提供给各个孔，并通过使用荧光，偏振，反射率等的光学技术（119）进行测定。 为此目的可以采用扫描激光系统（111）。 衬底可以在扫描期间旋转，以允许孔的一致询问，而不停止和开始旋转。 例如，也可以多次旋转重复地询问用于动力学研究的样品。

公开(公告)号：WO2005081801A2

公开(公告)日：2005-09-09

申请号：PCT/US2005/004055

申请日：2005-02-09

Applicant: BLUESHIFT BIOTECHNOLOGIES, INC. ,  CROMWELL, Evan, F. ,  MILLER, Steven, C. ,  SHUMATE, Christopher, B. ,  COMITA, Paul, B.

Inventor： CROMWELL, Evan, F. ,  MILLER, Steven, C. ,  SHUMATE, Christopher, B. ,  COMITA, Paul, B.

IPC: B01L3/00 ,  G01N1/10 ,  G01N21/25 ,  G01N21/64 ,  G01N35/02

CPC classification number: G01N21/253 ,  B01L3/5085 ,  B01L2300/0806 ,  G01N21/6408 ,  G01N21/6445 ,  G01N21/6452 ,  G01N35/028

Abstract: Methods and apparatus for assaying biological materials employ multi-well substrates as described herein. The substrates include a plurality of wells, typically each of several nanoliters volume or smaller having consistent dimensions and formed in a rigid substrate such as a glass disk. Each well may be provided with a circumferential lip to minimize crosstalk between wells and/or facilitate optical location of the individual wells during interrogation. Samples are provided to the individual wells and assayed by an optical technique employing fluorescence, polarization, reflectance, or the like. A scanning laser system may be employed for this purpose. The substrate may rotate during the scan to allow consistent interrogation of the wells without stopping and starting the rotation. Multiple rotations may also be employed repeatedly interrogate the samples for use in a kinetic study, for example.

Abstract translation: 用于测定生物材料的方法和装置采用本文所述的多孔底物。 衬底包括多个孔，通常具有一定尺寸的几个纳升体积或更小的孔，并形成在诸如玻璃盘的刚性衬底中。 每个孔可以设置有圆周唇缘，以最小化井之间的串扰和/或促使询问期间各个孔的光学定位。 将样品提供给各个孔，并通过使用荧光，极化，反射率等的光学技术进行测定。 为此目的可以采用扫描激光系统。 衬底可以在扫描期间旋转，以允许孔的一致询问，而不停止和开始旋转。 例如，也可以多次旋转重复地询问用于动力学研究的样品。

公开(公告)号：WO2010036819A3

公开(公告)日：2010-07-29

申请号：PCT/US2009058256

申请日：2009-09-24

Applicant: NETAPP INC ,  KANEVSKY ARKADY ,  MILLER STEVEN C

Inventor： KANEVSKY ARKADY ,  MILLER STEVEN C

IPC: G06F15/167 ,  G06F3/06 ,  G06F12/00 ,  G06F13/14

CPC classification number: G06F13/28 ,  G06F9/45558 ,  G06F2009/45583 ,  G06F2009/45587

Abstract: A processing system includes a plurality of virtual machines which have shared access to a non-volatile solid-state memory (NVSSM) subsystem, by using remote direct memory access (RDMA). The NVSSM subsystem can include flash memory and other types of non-volatile solid-state memory. The processing system uses scatter-gather lists to specify the RDMA read and write operations. Multiple reads or writes can be combined into a single RDMA read or write, respectively, which can then be decomposed and executed as multiple reads or writes, respectively, in the NVSSM subsystem. Memory accesses generated by a single RDMA read or write may be directed to different memory devices in the NVSSM subsystem, which may include different forms of non-volatile solid-state memory.

Abstract translation: 处理系统包括通过使用远程直接存储器访问（RDMA）具有对非易失性固态存储器（NVSSM）子系统的共享访问权的多个虚拟机。 NVSSM子系统可以包括闪存和其他类型的非易失性固态存储器。 处理系统使用分散收集列表来指定RDMA读写操作。 多个读取或写入可以分别组合成单个RDMA读取或写入，然后可以分别在NVSSM子系统中分解和执行多次读取或写入。 由单个RDMA读取或写入生成的存储器访问可以被引导到NVSSM子系统中的不同存储器件，其可以包括不同形式的非易失性固态存储器。

公开(公告)号：WO2010036819A2

公开(公告)日：2010-04-01

申请号：PCT/US2009/058256

申请日：2009-09-24

Applicant: NETAPP, INC. ,  KANEVSKY, Arkady ,  MILLER, Steven C.

Inventor： KANEVSKY, Arkady ,  MILLER, Steven C.

IPC: G06F15/167 ,  G06F3/06 ,  G06F12/00 ,  G06F13/14

CPC classification number: G06F13/28 ,  G06F9/45558 ,  G06F2009/45583 ,  G06F2009/45587

Abstract: A processing system includes a plurality of virtual machines which have shared access to a non-volatile solid-state memory (NVSSM) subsystem, by using remote direct memory access (RDMA). The NVSSM subsystem can include flash memory and other types of non-volatile solid-state memory. The processing system uses scatter-gather lists to specify the RDMA read and write operations. Multiple reads or writes can be combined into a single RDMA read or write, respectively, which can then be decomposed and executed as multiple reads or writes, respectively, in the NVSSM subsystem. Memory accesses generated by a single RDMA read or write may be directed to different memory devices in the NVSSM subsystem, which may include different forms of non-volatile solid-state memory.

Abstract translation: 通过使用远程直接存储器访问（RDMA），处理系统包括具有对非易失性固态存储器（NVSSM）子系统的共享访问的多个虚拟机。 NVSSM子系统可以包括闪存和其他类型的非易失性固态存储器。 处理系统使用分散 - 收集列表来指定RDMA读取和写入操作。 多个读取或写入可以分别组合成单个RDMA读取或写入，然后可以分别在NVSSM子系统中分解和执行多个读取或写入。 由单个RDMA读取或写入产生的存储器访问可以被引导至NVSSM子系统中的不同存储器设备，其可以包括不同形式的非易失性固态存储器。

公开(公告)号：WO2009102425A1

公开(公告)日：2009-08-20

申请号：PCT/US2009/000849

申请日：2009-02-11

Applicant: NETAPP, INC. ,  KIMMEL, Jeffrey, S. ,  KLEIMAN, Steven, R. ,  MILLER, Steven, C.

Inventor： KIMMEL, Jeffrey, S. ,  KLEIMAN, Steven, R. ,  MILLER, Steven, C.

IPC: G06F3/06

CPC classification number: G06F3/0647 ,  G06F3/061 ,  G06F3/0616 ,  G06F3/0643 ,  G06F3/0685 ,  G06F11/108 ,  G06F17/30185 ,  G06F17/30218 ,  G06F2211/109

Abstract: A hybrid media storage architecture has a log-structured file system configured to control a plurality of different storage media organized as hybrid storage media that cooperate to provide a total storage space of a storage system. The log-structured file system is configured to perform initial placement and migration of data, as well as fine-grain write allocation of the data, among storage space locations of the hybrid storage media to thereby improve the performance characteristics of the media. By defining and implementing heuristics and policies directed to, e.g., types of data, the file system may initially place data on any of the different media and thereafter migrate data between the media at fine granularity and without the need for manual enforcement.

Abstract translation: 混合媒体存储架构具有日志结构化文件系统，其被配置为控制组织为混合存储介质的多个不同的存储介质，所述混合存储介质协作以提供存储系统的总存储空间。 日志结构文件系统被配置为在混合存储介质的存储空间位置之间执行数据的初始放置和迁移以及数据的细粒度写入分配，从而提高媒体的性能特性。 通过定义和实现针对例如数据类型的启发式和策略，文件系统可以最初将数据放置在任何不同的介质上，然后以细粒度在介质之间迁移数据，而不需要手动执行。

公开(公告)号：WO2007084704A1

公开(公告)日：2007-07-26

申请号：PCT/US2007/001512

申请日：2007-01-19

Applicant: BLUESHIFT BIOTECHNOLOGIES, INC. ,  CROMWELL, Evan, F. ,  MILLER, Steven, C. ,  TRUJILLO, Robert, T. ,  COMITA, Paul, B.

Inventor： CROMWELL, Evan, F. ,  MILLER, Steven, C. ,  TRUJILLO, Robert, T. ,  COMITA, Paul, B.

IPC: G01N21/00 ,  B01L3/00

CPC classification number: B01L3/50853 ,  B01L2200/0684 ,  B01L2300/0654 ,  B01L2300/0829 ,  G01N21/0303 ,  G01N21/49

Abstract: Methods and apparatus for performing scatterometry measurements of biological samples as described herein. A substrate having formed therein one or more sample wells is provided. Each sample well is configured to hold a sample solution containing objects that are to be characterized based on their light scattering properties. One or more sample solutions are dispensed into the sample wells. A specular reflection reducing element is applied to at least some of the sample solutions in the sample wells to reduce a curvature of the meniscus of the sample solution and, therefore, to decrease reflections of light into one or more detectors. A light beam is directed from a light source onto the objects in the sample wells. Light scattered by the objects in the sample wells is collected and transmitted to one or more detectors. The signal from the detectors is analyzed to detect the one or more characteristics of the one or more samples .

Abstract translation: 如本文所述进行生物样品的散射测量的方法和装置。 提供了其中形成有一个或多个样品阱的衬底。 每个样品孔构造成容纳含有根据其光散射性质进行表征的物体的样品溶液。 将一种或多种样品溶液分配到样品孔中。 将镜面反射减少元件应用于样品阱中的至少一些样品溶液以减少样品溶液的弯月面的曲率，并因此减少光到一个或多个检测器中的反射。 将光束从光源引导到样品孔中的物体上。 被样品孔中的物体散射的光被收集并传输到一个或多个检测器。 分析来自检测器的信号以检测一个或多个样品的一个或多个特性。

公开(公告)号：WO2006014351A3

公开(公告)日：2006-02-09

申请号：PCT/US2005/023520

申请日：2005-07-01

Applicant: BLUESHIFT BIOTECHNOLOGIES, INC. ,  COMITA, Paul, B. ,  SHUMATE, Christopher, B. ,  MILLER, Steven, C. ,  CROMWELL, Evan, F.

Inventor： COMITA, Paul, B. ,  SHUMATE, Christopher, B. ,  MILLER, Steven, C. ,  CROMWELL, Evan, F.

IPC: C12Q1/68 ,  G01N33/53

Abstract: Methods, apparatus, and system, implementing and using techniques for detecting a presence of one or more target analytes in particular regions of interest of one or more samples. One or more samples including objects and one or more target analytes are provided. Some of the target analytes are labeled with a fluorophore and are bound to some of the objects in the samples. The samples are illuminated with fluorescence inducing light and fluorescent light is collected from one or more regions of the one or more samples. At least one anisotropy measurement of the samples is performed to identify regions of interest where one or more target analytes are bound to the objects. The collected fluorescent light from the regions of interest is analyzed to determine a presence of target analytes that are bound to the objects in the one or more samples.