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14 条记录 (耗时 0.011 秒)

    PROTEIN COMPLEX PURIFICATION
    4.
    发明申请
    PROTEIN COMPLEX PURIFICATION 审中-公开
    蛋白复合物纯化

    公开(公告)号：WO03095619A2

    公开(公告)日：2003-11-20

    申请号：PCT/US0314511

    申请日：2003-05-09

    申请人： MYRIAD PROTEOMICS INC ,  BONIFACE JOHN J ,  KERY VLADIMIR ,  PELTIER JOHN M ,  WEIR LAWRENCE ,  ROBBINS PAUL B ,  RODRIGUEZ MANUEL M

    发明人： BONIFACE JOHN J ,  KERY VLADIMIR ,  PELTIER JOHN M ,  WEIR LAWRENCE ,  ROBBINS PAUL B ,  RODRIGUEZ MANUEL M

    IPC分类号： C07K1/22 ,  C07K1/36 ,  C07K5/103 ,  C07K5/113 ,  G01N33/68 ,  C12N

    CPC分类号： G01N33/6842 ,  C07K1/22 ,  C07K1/36 ,  C07K5/101 ,  C07K5/1021 ,  G01N33/68 ,  G01N2500/00

    摘要： The present invention provides a method for isolating protein complexes from a cell, a cell or tissue lysate or an whole organism by employing a combined set of affinity tags of high affinity, specificity and ease of elution. The method involves using a known protein modified to contain more than one affinity tag separated by one or more specific protease cleavage sites, as bait, to isolate any interacting proteins or fragments thereof. The proteins or fragments thereof contained in the isolated complex can then be identified and the interacting partners can be used as new targets for diagnostic tools or the basis for the development of new compounds for therapeutic drug intervention.

    摘要翻译： 本发明提供了通过使用高亲和力，特异性和易于洗脱的组合的一组亲和标签从细胞，细胞或组织裂解物或整个生物体分离蛋白质复合物的方法。 该方法包括使用已知的已被修饰的蛋白质包含一个或多个特异性蛋白酶切割位点分离的多于一个的亲和标签作为诱饵，以分离任何相互作用的蛋白质或其片段。 然后可以鉴定分离的复合物中包含的蛋白质或其片段，并且相互作用的配偶体可以用作诊断工具的新靶标或用于开发用于治疗性药物干预的新化合物的基础。

    SELF-ALIGNING NOZZLE ARRAY FOR PIPETTE TIP ARRAY PLATE
    8.
    发明申请
    SELF-ALIGNING NOZZLE ARRAY FOR PIPETTE TIP ARRAY PLATE 审中-公开

    公开(公告)号：WO2002045857A3

    公开(公告)日：2002-06-13

    申请号：PCT/US2001/046319

    申请日：2001-12-04

    申请人： ROBBINS SCIENTIFIC CORPORATION ,  STANCHFIELD, Jim, E. ,  WRIGHT, Dave, J. ,  ROBINSON, David ,  ROBBINS, Paul, B. ,  CHRISTENSEN, Kenneth, J.

    发明人： STANCHFIELD, Jim, E. ,  WRIGHT, Dave, J. ,  ROBINSON, David ,  ROBBINS, Paul, B. ,  CHRISTENSEN, Kenneth, J.

    IPC分类号： G01N1/00

    摘要： An apparatus (10) for dispensing liquid in conjunction with an array of pipette tips (14), where each pipette tip (36) includes a socket portion (42). The liquid dispensing apparatus (10) includes tubes (25), each including a barrel (28) and a lower end (33), and a nozzle (34), each nozzle (34) having a shoulder (68) and a mouth (44) configured of appropriate diameter to enter into the socket portion (42) of each pipette tip (36), and form a liquid-tight seal therewith. A bottom plate (24) includes a number of through-holes (72) through which each of the nozzles passes (34). The through holes (72) are of larger diameter than the nozzles (34) so that limited lateral movement is allowed. A second embodiment (100) is convertible, so that separation of upper (73) and lower boundaries (75) may be decreased such that the upper boundary (73) contacts the shoulders (68) of said nozzles (34), to hold the nozzles (34) in fixed position, for use with pipette tip arrays (96) which are not bound together in fixed spatial relation to each other, but which are themselves allowed limited lateral movement. Both embodiments (10, 100) may further include a shucker plate (50) such that when the shucker plate (50) is made to travel away from the bottom plate (24) by an ejection mechanism (85), the pipette tips (36, 97) are pushed off of the nozzle mouths (44).

    COMPARTMENTALIZED MULTI-WELL CONTAINER
    9.
    发明申请
    COMPARTMENTALIZED MULTI-WELL CONTAINER 审中-公开
    隔音多层容器

    公开(公告)号：WO1997006890A1

    公开(公告)日：1997-02-27

    申请号：PCT/US1996012985

    申请日：1996-08-09

    申请人： ROBBINS SCIENTIFIC CORPORATION ,  ROBBINS, Paul, B.

    发明人： ROBBINS SCIENTIFIC CORPORATION

    IPC分类号： B01L03/00

    CPC分类号： B01L3/50851 ,  B01L3/5085 ,  B01L2300/0893

    摘要： A multi-well container (10) is provided in which a greater number of wells may exist than heretofore possible while still maintaining a standard multi-well plate tube array format and footprint. The multi-well container (10) is comprised of a rectangular array of tubes (12) in standard tube format held together by an integrally fashioned plate portion (16). The tubes (12) are subdivided by partitions or septa (14) which extend the height of the tubes (12). Each septum (14) may constitute a single wall or may be comprised of any number of fins (32) which are integral with internal tube surfaces (28). In a symmetrical design with four such fins (32), the fins (32) radiate at angular intervals of 90 degrees from a common central axis (34) with which all four fins (32) are integral. Thus, the septa (14) serve to compartmentalize each tube (12) into symmetrical quadrants of four smaller wells or sub-tubes (30). The preferred embodiment is directed toward usage in conjunction with PCR thermal cyclers, but the multi-well container (10) and the elements as are embodied therein are generally applicable to any laboratory procedure where multiple samples must be treated, evaluated, or stored.

    摘要翻译： 提供了一种多孔容器（10），其中可以存在比迄今为止更多的孔，同时仍保持标准的多孔板管阵列格式和占地面积。 多孔容器（10）由标准管形式的由整体形成的板部分（16）保持在一起的矩形管阵列（12）组成。 管（12）通过延伸管（12）的高度的隔板（14）细分。 每个隔膜（14）可以构成单个壁，或者可以由与内部管表面（28）成一体的任何数量的翅片（32）构成。 在具有四个这样的翅片（32）的对称设计中，翅片（32）以与所有四个翼片（32）为一体的公共中心轴线（34）成90度的角度间隔辐射。 因此，隔片（14）用于将每个管（12）分隔成四个较小孔或子管（30）的对称象限。 优选实施方案涉及与PCR热循环仪结合使用，但是多孔容器（10）和其中体现的元件通常可应用于必须处理，评估或储存多个样品的任何实验室程序。