摘要:
Sample holder including a support, an opening extending through the support and having a side wall formed by the support, a membrane disposed on the support and extending across the opening, and an insert open at both ends and positioned within the opening. The insert compresses the membrane against the side wall to form a liquid impervious seal. The insert may define, in combination with the membrane, a sample-receiving volume.
摘要:
A silicon-based sleeve type chemical reaction chamber (50) that combines doped silicon heating elements (57) and bulk silicon convective cooling elements (51, 52). A critical ratio of silicon and non-silicon based materials provides the thermal properties desired. The reaction chamber may be used for any chemical reaction, especially synthetic, thermal-cycling-based reactions such as the polymerase chain reaction (PCR) or ligase chain reaction.
摘要:
A microplate (10) and a method for manufacturing microplates. The microplate (10) is designed to reduce optical cross-talk during the assaying of samples. The microplate (10) comprises a plurality of wells (12) having light impermeable sidewalls (16), a layer of light permeable material forming a bottom (20) of the wells (12), and ribs (22) of light impermeable material extending through the layer of light permeable material. The method includes steps of inserting a plate of light permeable material into a mold cavity that includes sections shaped to form the sidewalls of the plurality of wells, injecting molten light impermeable material into the mold cavity, and cooling the light impermeable material to form the microplate with the light impermeable material forming the sidewalls of each of the plurality of wells and the plate of light permeable material forming the bottom wall of each of the plurality of wells.
摘要:
A silicon-based sleeve type chemical reaction chamber (41) that combines heaters, such as doped polysilicon for heating, and bulk silicon for convection cooling. The reaction chamber combines a critical ratio of silicon and silicon nitride to the volume of material to be heated (e.g., a liquid) in order to provide uniform heating, yet low power requirements. The reaction chamber will also allow the introduction of a secondary tube (45) (e.g., plastic) into the reaction sleeve that contains the reaction mixture thereby alleviating any potential materials incompatibility issues. The reaction chamber may be utilized in any chemical reaction system for synthesis or processing of organic, inorganic, or biochemical reactions, such as the polymerase chain reaction (PCR) and/or other DNA reactions, such as the ligase chain reaction, which are examples of a synthetic, thermal-cycling-based reaction. The reaction chamber may be used in synthesis instruments, particularly those for DNA amplification and synthesis.
摘要:
A container is provided comprising expanded porous polytetrafluoroethylene that is used to hold a matrix with analyte that is undergoing physical, thermal, or extraction analysis. The container may be of any shape but is preferable in tubular shape having an inlet side, a sealed side, and an interior space.
摘要:
This invention features methods and devices for rapidly, efficiently and conveniently synthesizing combinatorial libraries of chemical compounds. The present invention provides an efficient method for synthesizing N or N compounds. Specifically, a two-dimensional or three-dimensional conduit synthesis device is provided.
摘要:
A microtitration plate (40) has at least one sample container (45) and a lid (41) that covers the container openings when it is laid on the microtitration plate. The microtitration plate (40) and the lid (41) are made of a transparent material. The lid (41) is provided with downward-projecting shoulders (41) whose free end (43) has a flat closing surface (43). The downward-projecting shoulders (41) are arranged and designed in such a way that when the lid (41) is laid on the container (45) each shoulder (42) is associated to a container (45). The microtitration plate (40) is characterised in that liquid-tight walls that surround the opening of each container are designed on the plate (40) and delimit a space open at the top with a larger cross-section than that of the container opening. The flat closing surface (43) at the free end of the shoulder (42) covers at least the cross-section of the container opening and lies on the container opening when the lid is set on and completely lowered onto the container.
摘要:
An improved microfabricated apparatus for conducting a multiplicity of individual and simultaneous binding reactions is described. The apparatus comprises a substrate on which are located discrete and isolated sites for binding reactions. The apparatus is characterized by discrete and isolated regions that extend through said substrate and terminate on a second surface thereof such that when a test sample is allowed to the substrate, it is capable of penetrating through each such region during the course of said binding reaction. The apparatus is especially useful for sequencing by hybridization of DNA molecules.
摘要:
Apparatus and method for studying cellular processes comprise a vessel having a base including a layer comprising a scintillant substance and which is adapted for attachment and/or growth of cells. Cellular processes are examined by scintillation proximity assay using a reagent labeled with a radioisotope.
摘要:
A cartridge for holding an analytical sample during a diagnostic assay comprises a panel with a number of adjacent wells in its upper side. The wells have an upper opening and a lower opening, and the lower opening extends to the underside of the panel. A filter membrane extends across the lower openings of the wells so that liquids contained in the wells may be drawn through the filter, thereby concentrating suspended solids near the lower openings of the wells. The panel includes a supporting wall for supporting the panel on a non-integral, external surface, which does not form a part of the panel.