公开(公告)号：WO2014055778A2

公开(公告)日：2014-04-10

申请号：PCT/US2013/063298

申请日：2013-10-03

申请人： AGRIVIDA, INC.

发明人： SHEN, Binzhang ,  DONALD, Jason ,  RAAB, Michael

IPC分类号： C12P21/02

CPC分类号： C12N15/635 ,  C07K14/81 ,  C12N9/20 ,  C12N9/2437 ,  C12N9/248 ,  C12N9/50 ,  C12N9/54 ,  C12N9/64 ,  C12N15/62 ,  C12N15/63 ,  C12Y304/21062

摘要： Methods for expressing multiple proteins by constructing transformation vectors that include multiprotein expression cassettes and transforming hosts with vectors and by engineering hosts expressing multiprotein units are provided. Multiprotein units that include multiple proteins fused to modified inteins capable of effecting splicing of the multiprotein units are described. Expression cassettes that include nucleic acids encoding multiprotein units and hosts including the expression cassettes are also provided.

摘要翻译： 提供了通过构建包含多蛋白表达盒的转化载体和用载体转化宿主和通过工程表达多蛋白单位的宿主来表达多种蛋白质的方法。 描述了包含与能够影响多蛋白单位剪接的修饰的内含肽融合的多种蛋白质的多蛋白单位。 还提供了包括编码多蛋白单位的核酸和包括表达盒的宿主的表达盒。

公开(公告)号：WO2011057101A1

公开(公告)日：2011-05-12

申请号：PCT/US2010/055669

申请日：2010-11-05

申请人： AGRIVIDA, INC. ,  SHEN, Binzhang ,  LAZAR, Gabor ,  DE LA VEGA, Humberto ,  LESSARD, Phillip ,  RAAB, Michael R. ,  APGAR, James

发明人： SHEN, Binzhang ,  LAZAR, Gabor ,  DE LA VEGA, Humberto ,  LESSARD, Phillip ,  RAAB, Michael R. ,  APGAR, James

IPC分类号： C07K14/415 ,  A01H1/00

CPC分类号： C12N9/2482 ,  C12N9/2437 ,  C12N9/248 ,  C12N15/8216 ,  C12N15/8241 ,  C12N15/8246 ,  C12Y302/01004 ,  C12Y302/01008

摘要： Intein modified proteins, isolated nucleic acids encoding intein modified proteins, fragments of intein modified proteins, isolated nucleic acids encoding fragments of intein modified proteins, transgenic plants containing any of the foregoing, and antibodies recognizing epitopes on intein modified proteins are provided

摘要翻译： 提供了编码内含肽修饰蛋白质的分离核酸，内含肽修饰蛋白质片段，编码内含肽修饰蛋白质片段的分离核酸，含有前述内容物的转基因植物，以及识别内含肽修饰蛋白质上的抗原决定簇的抗体。

公开(公告)号：WO2017049094A1

公开(公告)日：2017-03-23

申请号：PCT/US2016/052147

申请日：2016-09-16

申请人： AGRIVIDA, INC.

发明人： RAAB, R. Michael ,  LAZAR, Gabor ,  SHEN, Binzhang

IPC分类号： C07K14/00 ,  C07K4/06 ,  C07K14/245 ,  C07K14/38 ,  C07K14/39 ,  C12N15/62 ,  C12N15/10 ,  C12N15/04 ,  C12N15/52 ,  C12N15/80 ,  C12N15/65 ,  C12N9/16 ,  A23K10/14

CPC分类号： A23K10/14 ,  A23K20/189 ,  C07K14/00 ,  C07K14/245 ,  C07K14/38 ,  C07K14/39 ,  C12N9/16 ,  C12N15/80 ,  C12N15/8257

摘要： Methods for enhancing phytase thermal stability by fusing binding elements to target phytases are provided. Engineered phytases that include binding elements fused to target phytases to cause cyclization of the engineered phytases and enhance thermal stability of the target phytases are described. Engineered nucleic acids encoding engineered phytases and hosts engineered to express engineered nucleic acids are also provided. Methods for incorporating engineered phytases in animal feed and animal feed including the same are described.

摘要翻译： 提供了通过将结合元件与靶植酸酶融合来提高植酸酶热稳定性的方法。 描述了包括与目标植酸酶融合以产生工程化植酸酶环化并增强靶植酸酶的热稳定性的结合元件的工程植酸酶。 还提供了编码工程化植酸酶的工程化核酸和工程化以表达工程化核酸的宿主。 描述了在动物饲料和包含其中的动物饲料中掺入工程化植酸酶的方法。

公开(公告)号：WO2016069774A1

公开(公告)日：2016-05-06

申请号：PCT/US2015/057862

申请日：2015-10-28

申请人： AGRIVIDA, INC.

发明人： RAAB, Michael, R. ,  SHEN, Binzhang ,  LAZAR, Gabor

IPC分类号： C12N9/50 ,  C12N9/54 ,  C12N15/62 ,  C11D3/386

CPC分类号： C11D3/386 ,  C07K2319/00 ,  C07K2319/92 ,  C12N9/54

摘要： Methods for producing a trans-splicing intein-modified protease with enhanced solubility and regulating its activity are described. Intein-modified proteases having enhanced solubility and polynucleotides encoding the same are provided. Methods of storing trans-splicing proteases are also described.

摘要翻译： 描述了制备具有增强的溶解度并调节其活性的反式拼接内蛋白修饰的蛋白酶的方法。 提供具有增强的溶解度的蛋白质修饰蛋白酶和编码其的多核苷酸。 还描述了存储转拼蛋白酶的方法。

公开(公告)号：WO2014055782A1

公开(公告)日：2014-04-10

申请号：PCT/US2013/063304

申请日：2013-10-03

申请人： AGRIVIDA, INC.

发明人： RAAB, R. Michael ,  SHEN, Binzhang ,  LAZAR, Gabor ,  DE LA VEGA, Humberto

IPC分类号： C12N15/00 ,  C12P21/06 ,  C12N9/64

CPC分类号： C12N15/635 ,  C07K14/81 ,  C12N9/20 ,  C12N9/2437 ,  C12N9/248 ,  C12N9/50 ,  C12N9/54 ,  C12N9/64 ,  C12N15/62 ,  C12N15/63 ,  C12Y304/21062

摘要： Methods for producing intein-modified proteases are provided. Expression cassettes and vectors for using to genetically engineer hosts are described. Hosts genetically engineered to express one or more intein-modified proteases using expression cassettes and vectors of the invention are also provided. Methods to produce a protease and regulate its activity are described.

摘要翻译： 提供了产生内蛋白修饰蛋白酶的方法。 描述了用于遗传工程主机的表达盒和载体。 还提供了使用本发明的表达盒和载体进行遗传工程改造以表达一种或多种内含肽修饰的蛋白酶的宿主。 描述了产生蛋白酶并调节其活性的方法。

公开(公告)号：WO2012027395A3

公开(公告)日：2012-03-01

申请号：PCT/US2011/048847

申请日：2011-08-23

申请人： AGRIVIDA, INC. ,  SHEN, Binzhang ,  APGAR, James ,  BOUGRI, Oleg ,  RAAB, R. Michael

发明人： SHEN, Binzhang ,  APGAR, James ,  BOUGRI, Oleg ,  RAAB, R. Michael

IPC分类号： C12N15/82 ,  A01H5/00 ,  C12N9/26

摘要： In planta consolidated bioprocessing has the advantages of decreasing biomass pretreatment costs, utilizing excess plant protein production capacity for enzyme production, and decreasing mass transfer resistance of enzyme diffusion to its substrate. However, in planta expression of cell wall degrading (CWD) enzymes often leads to detrimental plant phenotypes that impact crop yield. To provide in planta CWD enzyme activity without any adverse phenotype, a thermostable xylanase, XynB (EC 3.2.1.8), was engineered with a thermoregulated intein, Tth-HB27 DnaE-1 (Tth intein), that controls its hydrolytic activity through conditional intein splicing. Maize plants expressing the heat inducible Tth intein-modified XynB developed normally, yet possessed enhanced post harvest glucose production from dried corn stover. Expression of CWD enzymes as dormant, intein-modified proteins that can be activated by heat treatment after harvest provides the basis for developing a novel cellulosic processing trait in plants.

公开(公告)号：WO2011057163A2

公开(公告)日：2011-05-12

申请号：PCT/US2010/055751

申请日：2010-11-05

申请人： AGRIVIDA, INC. ,  SHEN, Binzhang ,  LAZAR, Gabor ,  DE LA VEGA, Humberto ,  APGAR, James ,  LESSARD, Phillip ,  RAAB, R., Michael

发明人： SHEN, Binzhang ,  LAZAR, Gabor ,  DE LA VEGA, Humberto ,  APGAR, James ,  LESSARD, Phillip ,  RAAB, R., Michael

IPC分类号： C12Q1/34 ,  C12N9/14 ,  C12N9/42 ,  C12N15/63 ,  A01H5/00

CPC分类号： C12N9/2482 ,  C12N9/2437 ,  C12N9/248 ,  C12N15/8216 ,  C12N15/8241 ,  C12N15/8246 ,  C12Y302/01004 ,  C12Y302/01008

摘要： A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-β-sheet junction or a loop-α- helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.

摘要翻译： 提供了一种预测蛋白质中的内含肽插入位点将导致切换表型的方法。 该方法包括鉴定蛋白内的多个C / T / S位点; 在多个C / T / S /位点中选择由支持向量机排列为0.75或更高的那些，在蛋白质的活性位点的10埃内，以及在ß-片结或环上 - 螺旋结。 还提供了控制蛋白质活性的方法和包含具有受控活性的蛋白质的宿主。 此外，提供了内含肽修饰的蛋白质和含有内含肽修饰的蛋白质的植物。