公开(公告)号：WO2012122308A2

公开(公告)日：2012-09-13

申请号：PCT/US2012/028132

申请日：2012-03-07

Applicant: RAAB, R. Michael ,  ZHANG, Dongcheng ,  BOUGRI, Oleg ,  AGRIVIDA, INC.

Inventor： RAAB, R. Michael ,  ZHANG, Dongcheng ,  BOUGRI, Oleg

IPC: G06F1/26

CPC classification number: C12N15/8246 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/248 ,  C12N15/8216 ,  C12P19/14 ,  C12Y302/01004 ,  C12Y302/01008 ,  C12Y302/01021 ,  Y02E50/10 ,  Y02E50/30

Abstract: Methods for consolidated pretreatment and hydrolysis of genetically engineered plants expressing cell wall degrading enzymes are provided. Expression cassettes and vectors for making transgenic plants are described. Plants engineered to express one or more cell wall degrading enzymes using expression cassettes and vectors of the invention are also provided.

Abstract translation: 提供了表达细胞壁降解酶的遗传工程植物的综合预处理和水解方法。 描述了用于制备转基因植物的表达盒和载体。 还提供了使用本发明的表达盒和载体表达一种或多种细胞壁降解酶的植物。

公开(公告)号：WO2014078588A1

公开(公告)日：2014-05-22

申请号：PCT/US2013/070184

申请日：2013-11-14

Applicant: AGRIVIDA, INC.

Inventor： LESSARD, Philip A. ,  LANAHAN, Michael ,  SAMOYLOV, Vladimir ,  BOUGRI, Oleg ,  EMERY, Jonas ,  RAAB, R. Michael ,  ZHANG, Dongcheng

IPC: C12N15/82 ,  C12N15/87 ,  C07H21/04

CPC classification number: C12N15/8245

Abstract: Genetically engineered plants having altered levels of one or more starch regulation enzymes and a polysaccharide degrading enzyme are provided. Methods of genetically engineering plants to express products altering expression of one or more starch regulation enzymes and polysaccharide degrading degrading enzymes, and genetic constructs are provided. Methods of agricultural processing and animal feed using the genetically engineered plants are described.

Abstract translation: 提供具有改变水平的一种或多种淀粉调节酶和多糖降解酶的遗传工程植物。 提供了植物表达改变一种或多种淀粉调节酶和多糖降解酶的表达产物的遗传构建体的方法。 描述了使用遗传工程植物的农业加工和动物饲料的方法。

公开(公告)号：WO2018222578A1

公开(公告)日：2018-12-06

申请号：PCT/US2018/034856

申请日：2018-05-29

Applicant: AGRIVIDA, INC.

Inventor： RAAB, R. Michael ,  BOUGRI, Oleg ,  PARKER, Matthew ,  LESSARD, Philip A.

IPC: A01H5/00 ,  A61K38/20 ,  C07K14/54 ,  C07K16/02 ,  A61K39/395 ,  C07K16/24

Abstract: The transgenic plants expressing one or more antagonist IL-10R peptides and anti-IL-10 single domain antibodies that stimulate or modulate the immune system and improve gastrointestinal physiology of an animal fed the transgenic plants or tissues thereof and the genes encoding the antagonist IL-10R peptides and anti-IL-10 single domain antibodies are described. The animal feed additives and animal feed incorporating the transgenic plants or tissues thereof are also described. Methods of stimulating or modulating an animal's immune system, improving an animal's gastrointestinal physiology, improving animal performance by using the transgenic plants or tissues thereof, and treating animals infected with a gastrointestinal pathogen are provided.

公开(公告)号：WO2018089264A1

公开(公告)日：2018-05-17

申请号：PCT/US2017/059903

申请日：2017-11-03

Applicant: AGRIVIDA, INC.

Inventor： RAAB, R. Michael ,  BOUGRI, Oleg ,  LESSARD, Philip A. ,  BONIN, Christopher ,  LANAHAN, Michael B.

IPC: A23K20/26 ,  A23K50/75 ,  C07H21/04 ,  C07K14/415 ,  C12N9/16 ,  C12N9/24

Abstract: Methods and compositions are described for producing a phytase in transgenic maize plants and then incorporating parts of the transgenic maize plants in animal feed. The feed phytase enzyme displays activity across a broad pH range, and tolerance to temperatures that are often encountered during the process of preparing animal feeds. Methods of producing an animal feed that incorporate the transgenic maize plants, parts thereof or plant derived phytases, as well as methods of promoting the release of inorganic phosphate from a phytic acid in an animal, producing an animal meat, or reducing the ratio of intake of an animal feed per weight of the animal meat by feeding an animal with the animal feed incorporating transgenic maize plants are provided.

Abstract translation: 描述了用于在转基因玉米植物中产生植酸酶，然后将部分转基因玉米植物并入动物饲料中的方法和组合物。 饲料植酸酶在广泛的pH范围内显示出活性，并且耐受制备动物饲料过程中经常遇到的温度。 生产掺入转基因玉米植物，其部分或植物衍生的肌醇六磷酸酶的动物饲料的方法，以及促进动物中从植酸中释放无机磷酸盐，生产动物肉或降低摄入比率的方法 本发明提供了通过用掺入了转基因玉米植物的动物饲料给动物喂食每动物肉重量的动物饲料。

公开(公告)号：WO2017049094A1

公开(公告)日：2017-03-23

申请号：PCT/US2016/052147

申请日：2016-09-16

Applicant: AGRIVIDA, INC.

Inventor： RAAB, R. Michael ,  LAZAR, Gabor ,  SHEN, Binzhang

IPC: C07K14/00 ,  C07K4/06 ,  C07K14/245 ,  C07K14/38 ,  C07K14/39 ,  C12N15/62 ,  C12N15/10 ,  C12N15/04 ,  C12N15/52 ,  C12N15/80 ,  C12N15/65 ,  C12N9/16 ,  A23K10/14

CPC classification number: A23K10/14 ,  A23K20/189 ,  C07K14/00 ,  C07K14/245 ,  C07K14/38 ,  C07K14/39 ,  C12N9/16 ,  C12N15/80 ,  C12N15/8257

Abstract: Methods for enhancing phytase thermal stability by fusing binding elements to target phytases are provided. Engineered phytases that include binding elements fused to target phytases to cause cyclization of the engineered phytases and enhance thermal stability of the target phytases are described. Engineered nucleic acids encoding engineered phytases and hosts engineered to express engineered nucleic acids are also provided. Methods for incorporating engineered phytases in animal feed and animal feed including the same are described.

Abstract translation: 提供了通过将结合元件与靶植酸酶融合来提高植酸酶热稳定性的方法。 描述了包括与目标植酸酶融合以产生工程化植酸酶环化并增强靶植酸酶的热稳定性的结合元件的工程植酸酶。 还提供了编码工程化植酸酶的工程化核酸和工程化以表达工程化核酸的宿主。 描述了在动物饲料和包含其中的动物饲料中掺入工程化植酸酶的方法。

公开(公告)号：WO2016054039A1

公开(公告)日：2016-04-07

申请号：PCT/US2015/052940

申请日：2015-09-29

Applicant: AGRIVIDA, INC.

Inventor： RAAB, R. Michael ,  LANAHAN, Michael ,  BONIN, Christopher ,  BOUGRI, Oleg

IPC: C12N15/82 ,  A23K10/00

CPC classification number: C12N15/8245 ,  C12N9/1294 ,  C12N15/8218

Abstract: Genetically engineered plants expressing altered Glucan Water Dikinase and having elevated levels of starch are provided. Methods of genetically engineering plants to express altered Glucan Water Dikinase, and genetic constructs are provided. Methods of breeding genetically engineered plants homozygous for a mutated gene encoding an altered Glucan Water Dikinase are described. Methods of agricultural processing and animal feed using the genetically engineered plants are also provided.

Abstract translation: 提供表达改变的葡聚糖水定影素并具有升高的淀粉水平的转基因植物。 提供植物表达改变的葡聚糖水定点酶的方法和基因构建体。 描述了对编码改变的葡聚糖水定点酶的突变基因纯合的遗传工程植物的方法。 还提供了使用遗传工程植物的农业加工和动物饲料的方法。

公开(公告)号：WO2016187513A1

公开(公告)日：2016-11-24

申请号：PCT/US2016/033472

申请日：2016-05-20

Applicant: AGRIVIDA, INC.

Inventor： LI, Xuemei ,  RAAB, R. Michael

IPC: C12Q1/42 ,  C12Q1/34

CPC classification number: C12Q1/34 ,  C12Q1/42

Abstract: Methods of increasing extraction of enzymes from animal feed and measuring enzyme activity are described herein.

Abstract translation: 本文描述了增加动物饲料中酶的提取和测定酶活性的方法。

公开(公告)号：WO2016183467A1

公开(公告)日：2016-11-17

申请号：PCT/US2016/032418

申请日：2016-05-13

Applicant: AGRIVIDA, INC.

Inventor： RAAB, R. Michael ,  BOUGRI, Oleg ,  LI, Xuemei

IPC: C07K14/415 ,  C12N15/00 ,  C12N15/52 ,  C12N9/24

CPC classification number: C12N9/24 ,  C12N15/8242 ,  C12Q1/6895 ,  C12Q2600/13

Abstract: Methods and compositions are described for producing a glucanase in transgenic plants and then incorporating parts of the transgenic plants in animal feed. The feed glucanase enzyme displays activity across a broad pH range, and tolerance to temperatures that are often encountered during the process of preparing animal feeds. Producing the enzyme in the transgenic plant enhances the thermal stability of the enzyme.

Abstract translation: 描述了用于在转基因植物中产生葡聚糖酶的方法和组合物，然后将部分转基因植物掺入动物饲料中。 饲料葡聚糖酶显示在宽的pH范围内的活性，以及​​在制备动物饲料的过程中经常遇到的温度的耐受性。 在转基因植物中产生酶增强酶的热稳定性。

公开(公告)号：WO2014055782A1

公开(公告)日：2014-04-10

申请号：PCT/US2013/063304

申请日：2013-10-03

Applicant: AGRIVIDA, INC.

Inventor： RAAB, R. Michael ,  SHEN, Binzhang ,  LAZAR, Gabor ,  DE LA VEGA, Humberto

IPC: C12N15/00 ,  C12P21/06 ,  C12N9/64

CPC classification number: C12N15/635 ,  C07K14/81 ,  C12N9/20 ,  C12N9/2437 ,  C12N9/248 ,  C12N9/50 ,  C12N9/54 ,  C12N9/64 ,  C12N15/62 ,  C12N15/63 ,  C12Y304/21062

Abstract: Methods for producing intein-modified proteases are provided. Expression cassettes and vectors for using to genetically engineer hosts are described. Hosts genetically engineered to express one or more intein-modified proteases using expression cassettes and vectors of the invention are also provided. Methods to produce a protease and regulate its activity are described.

Abstract translation: 提供了产生内蛋白修饰蛋白酶的方法。 描述了用于遗传工程主机的表达盒和载体。 还提供了使用本发明的表达盒和载体进行遗传工程改造以表达一种或多种内含肽修饰的蛋白酶的宿主。 描述了产生蛋白酶并调节其活性的方法。

公开(公告)号：WO2012027395A3

公开(公告)日：2012-03-01

申请号：PCT/US2011/048847

申请日：2011-08-23

Applicant: AGRIVIDA, INC. ,  SHEN, Binzhang ,  APGAR, James ,  BOUGRI, Oleg ,  RAAB, R. Michael

Inventor： SHEN, Binzhang ,  APGAR, James ,  BOUGRI, Oleg ,  RAAB, R. Michael

IPC: C12N15/82 ,  A01H5/00 ,  C12N9/26

Abstract: In planta consolidated bioprocessing has the advantages of decreasing biomass pretreatment costs, utilizing excess plant protein production capacity for enzyme production, and decreasing mass transfer resistance of enzyme diffusion to its substrate. However, in planta expression of cell wall degrading (CWD) enzymes often leads to detrimental plant phenotypes that impact crop yield. To provide in planta CWD enzyme activity without any adverse phenotype, a thermostable xylanase, XynB (EC 3.2.1.8), was engineered with a thermoregulated intein, Tth-HB27 DnaE-1 (Tth intein), that controls its hydrolytic activity through conditional intein splicing. Maize plants expressing the heat inducible Tth intein-modified XynB developed normally, yet possessed enhanced post harvest glucose production from dried corn stover. Expression of CWD enzymes as dormant, intein-modified proteins that can be activated by heat treatment after harvest provides the basis for developing a novel cellulosic processing trait in plants.