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    SIGNAL SEQUENCES AND CO-EXPRESSED CHAPERONES FOR IMPROVING PROTEIN PRODUCTION IN A HOST CELL
    1.
    发明申请
    SIGNAL SEQUENCES AND CO-EXPRESSED CHAPERONES FOR IMPROVING PROTEIN PRODUCTION IN A HOST CELL 审中-公开
    用于改善宿主细胞中蛋白质生产的信号序列和共同表达的CHAPERON

    公开(公告)号：WO2009058956A1

    公开(公告)日：2009-05-07

    申请号：PCT/US2008/081721

    申请日：2008-10-30

    Applicant: DANISCO US, INC. ,  BAO, Kai ,  WANG, Huaming

    Inventor： BAO, Kai ,  WANG, Huaming

    IPC: C12N9/02 ,  C12N9/34 ,  C12N9/42 ,  C12N9/58 ,  C12N15/62 ,  C12N15/80 ,  C12P21/02

    CPC classification number: C12N9/2428 ,  C07K2319/02 ,  C12N9/0061 ,  C12N9/2437 ,  C12N9/58 ,  C12N15/62 ,  C12N15/80 ,  C12P21/02 ,  C12Y110/03002 ,  C12Y302/01091

    Abstract: The invention provides methods and compositions for improved protein production. The method comprises the steps of: (a) introducing into a host cell a first nucleic acid sequence comprising a signal sequence operably linked to a desired protein sequence; (b) expressing the first nucleic acid sequence; (c) co-expressing a second nucleic acid sequence encoding a chaperone or foldase selected from the group consisting of bip1, ero1, pdi1, tig1, prp1,ppi1, ppi2, prp3, prp4 , calnexin, and Ihs1 ; and (d) collecting the desired protein secreted from the host cell. The first nucleic acid sequence optionally comprises an enzyme sequence between the signal sequence and the desired protein sequence.

    Abstract translation: 本发明提供了改进蛋白质生产的方法和组合物。 该方法包括以下步骤：（a）向宿主细胞中引入包含可操作地连接到所需蛋白质序列的信号序列的第一核酸序列; （b）表达第一核酸序列; （c）共表达编码选自bip1，ero1，pdi1，tig1，prp1，ppi1，ppi2，prp3，prp4，calnexin和Ihs1的伴侣或折叠酶的第二核酸序列; 和（d）收集从宿主细胞分泌的所需蛋白质。 第一核酸序列任选地包含信号序列和所需蛋白质序列之间的酶序列。

    FILAMENTOUS FUNGI WITH INACTIVATED PROTEASE GENES FOR ALTERED PROTEIN PRODUCTION
    2.
    发明申请
    FILAMENTOUS FUNGI WITH INACTIVATED PROTEASE GENES FOR ALTERED PROTEIN PRODUCTION 审中-公开
    FILAMENTOUS FUNGI WITH INVTIVED PROTEASE GENES FOR ALTERED PROTEIN PRODUCTION

    公开(公告)号：WO2009126463A2

    公开(公告)日：2009-10-15

    申请号：PCT/US2009/038626

    申请日：2009-03-27

    Applicant: DANISCO US INC., GENENCOR DIVISION ,  WANG, Huaming

    Inventor： WANG, Huaming

    IPC: C12N1/15 ,  C12N15/80 ,  C12N15/52

    CPC classification number: C12P21/00 ,  C07K14/38 ,  C12N9/00 ,  C12N9/62 ,  C12N15/80 ,  C12P21/02

    Abstract: The invention relates to a filamentous fungal cell (e.g., Aspergillus sp.) comprising at least one inactivated protease gene chosen from apsB, a homolog of apsB, cpsA, a homolog cpsA, and combinations thereof. Nucleic acids and methods for making the inactivated mutant filamentous fungal cells are provided as well as methods for using the cells for the altered production of endogenous or heterologous proteins of interest.

    Abstract translation: 本发明涉及包含至少一种选自apsB的灭活蛋白酶基因，apsB同源物，cpsA，同系物cpsA及其组合的丝状真菌细胞（例如曲霉属）。 提供用于制备灭活的突变丝状真菌细胞的核酸和方法以及使用细胞改变产生感兴趣的内源或异源蛋白质的方法。

    LACCASE VARIANTS HAVING INCREASED EXPRESSION AND/OR ACTIVITY
    4.
    发明申请
    LACCASE VARIANTS HAVING INCREASED EXPRESSION AND/OR ACTIVITY 审中-公开
    具有增加的表达和/或活动的LACCASE变量

    公开(公告)号：WO2012138474A1

    公开(公告)日：2012-10-11

    申请号：PCT/US2012/029786

    申请日：2012-03-20

    Applicant: DANISCO US INC. ,  WANG, Huaming ,  WARD, Michael

    Inventor： WANG, Huaming ,  WARD, Michael

    IPC: C12N9/02

    CPC classification number: C12N9/0061 ,  D06L4/40

    Abstract: The present compositions, methods, and systems, relating to variant laccase enzymes that demonstrate increased expression and/or activity compared to a reference parental laccase enzyme. The variant enzymes include mutations that affect glycosylation, surface charge, or surface hydrophobicity, resulting in improved enzyme expression and/or enzyme activity.

    Abstract translation: 与参照亲本漆酶相比表现出增加的表达和/或活性的变体漆酶的本发明组合物，方法和系统。 变体酶包括影响糖基化，表面电荷或表面疏水性的突变，导致改善的酶表达和/或酶活性。

    NEUTRAL CELLULASE CATALYTIC CORE AND METHOD OF PRODUCING SAME
    10.
    发明申请
    NEUTRAL CELLULASE CATALYTIC CORE AND METHOD OF PRODUCING SAME 审中-公开
    中性纤维素酶催化核及其生产方法

    公开(公告)号：WO2006071598A1

    公开(公告)日：2006-07-06

    申请号：PCT/US2005/045859

    申请日：2005-12-16

    Applicant: GENENCOR INTERNATIONAL, INC. ,  BAO, Kai ,  WANG, Huaming

    Inventor： BAO, Kai ,  WANG, Huaming

    IPC: C12N9/42 ,  C11D3/386 ,  D06M16/00 ,  D21C5/00 ,  A23K1/165 ,  C12N15/55

    CPC classification number: C12N9/2437 ,  A23K10/14 ,  A23K20/189 ,  C11D3/38645 ,  D06M16/003 ,  D06M2101/06 ,  D06M2200/50 ,  D06P1/38 ,  D21C5/005

    Abstract: The present invention relates to the cloning and high level expression of novel cellulase proteins or derivatives thereof in the in a host cell. Further aspects of the present invention relate to transformants that express the novel cellulases, and expression vectors comprising the DNA gene fragments or variants thereof that code for the novel cellulases derived from Actinomycete using genetic engineering techniques. The present invention is also directed to novel cellulase compositions and methods of use therefore in industrial processes. In particular, the present invention is related to treating textiles with a novel cellulase derived from Actinomycete spp . The present invention also relates to the use of cellulase derived from Actinomycete spp . to enhance the digestibility of animal feed, in detergents, in the treatment of pulp and paper and in the production of starch and treatment of by-products thereof.

    Abstract translation: 本发明涉及在宿主细胞中克隆和高水平表达新的纤维素酶蛋白或其衍生物。 本发明的其它方面涉及表达新型纤维素酶的转化体，以及包含使用遗传工程技术编码来自放线菌的新型纤维素酶的DNA基因片段或其变体的表达载体。 本发明还涉及新型纤维素酶组合物和因此在工业过程中使用的方法。 特别地，本发明涉及用衍生自放线菌属的新型纤维素酶处理纺织品。 本发明还涉及来自放线菌属的纤维素酶的用途。 以提高动物饲料，洗涤剂，纸浆和纸张的处理和淀粉生产的消化率及其副产物的处理。

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