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    PHENOL OXIDIZING ENZYMES FROM FUNGHI
    6.
    发明申请
    PHENOL OXIDIZING ENZYMES FROM FUNGHI 审中-公开
    来自真菌的苯酚氧化酶

    公开(公告)号：WO0037654A3

    公开(公告)日：2000-09-14

    申请号：PCT/US9931009

    申请日：1999-12-20

    Applicant: GENENCOR INT ,  WANG HUAMING ,  BODIE ELIZABETH A

    Inventor： WANG HUAMING ,  BODIE ELIZABETH A

    IPC: C11D3/386 ,  C12N9/02 ,  C12N15/53

    CPC classification number: C12N9/0004 ,  C11D3/38654 ,  Y10S435/911

    Abstract: Disclosed herein are detergent compositions comprising novel phenol oxidizing enzymes encoded by nucleic acid capable of hybridizing to the nucleic acid having the sequence as shown in SEQ ID NO:1 and in particular those obtainable from fungus, in particular from Bipolaris spicifera, Curvularia pallescens and Amerosporium atrum. The present invention provides expression vectors and host cells comprising nucleic acid encoding phenol oxidizing enzymes, methods for producing the phenol oxidizing enzymes as well as methods for constructing expression hosts.

    Abstract translation: 本文公开了包含由能够与具有SEQ ID NO：1所示序列的核酸杂交的核酸编码的新型酚氧化酶的洗涤剂组合物，特别是可从真菌，特别是来自Bipolaris spicifera，Curvularia pallescens和Amerosporium atrum。 本发明提供了包含编码酚氧化酶的核酸的表达载体和宿主细胞，用于生产酚氧化酶的方法以及构建表达宿主的方法。

    LACCASE VARIANTS HAVING INCREASED EXPRESSION AND/OR ACTIVITY
    7.
    发明申请
    LACCASE VARIANTS HAVING INCREASED EXPRESSION AND/OR ACTIVITY 审中-公开
    具有增加的表达和/或活动的LACCASE变量

    公开(公告)号：WO2012138474A1

    公开(公告)日：2012-10-11

    申请号：PCT/US2012/029786

    申请日：2012-03-20

    Applicant: DANISCO US INC. ,  WANG, Huaming ,  WARD, Michael

    Inventor： WANG, Huaming ,  WARD, Michael

    IPC: C12N9/02

    CPC classification number: C12N9/0061 ,  D06L4/40

    Abstract: The present compositions, methods, and systems, relating to variant laccase enzymes that demonstrate increased expression and/or activity compared to a reference parental laccase enzyme. The variant enzymes include mutations that affect glycosylation, surface charge, or surface hydrophobicity, resulting in improved enzyme expression and/or enzyme activity.

    Abstract translation: 与参照亲本漆酶相比表现出增加的表达和/或活性的变体漆酶的本发明组合物，方法和系统。 变体酶包括影响糖基化，表面电荷或表面疏水性的突变，导致改善的酶表达和/或酶活性。

    SIGNAL SEQUENCES AND CO-EXPRESSED CHAPERONES FOR IMPROVING PROTEIN PRODUCTION IN A HOST CELL
    8.
    发明申请
    SIGNAL SEQUENCES AND CO-EXPRESSED CHAPERONES FOR IMPROVING PROTEIN PRODUCTION IN A HOST CELL 审中-公开
    用于改善宿主细胞中蛋白质生产的信号序列和共同表达的CHAPERON

    公开(公告)号：WO2009058956A1

    公开(公告)日：2009-05-07

    申请号：PCT/US2008/081721

    申请日：2008-10-30

    Applicant: DANISCO US, INC. ,  BAO, Kai ,  WANG, Huaming

    Inventor： BAO, Kai ,  WANG, Huaming

    IPC: C12N9/02 ,  C12N9/34 ,  C12N9/42 ,  C12N9/58 ,  C12N15/62 ,  C12N15/80 ,  C12P21/02

    CPC classification number: C12N9/2428 ,  C07K2319/02 ,  C12N9/0061 ,  C12N9/2437 ,  C12N9/58 ,  C12N15/62 ,  C12N15/80 ,  C12P21/02 ,  C12Y110/03002 ,  C12Y302/01091

    Abstract: The invention provides methods and compositions for improved protein production. The method comprises the steps of: (a) introducing into a host cell a first nucleic acid sequence comprising a signal sequence operably linked to a desired protein sequence; (b) expressing the first nucleic acid sequence; (c) co-expressing a second nucleic acid sequence encoding a chaperone or foldase selected from the group consisting of bip1, ero1, pdi1, tig1, prp1,ppi1, ppi2, prp3, prp4 , calnexin, and Ihs1 ; and (d) collecting the desired protein secreted from the host cell. The first nucleic acid sequence optionally comprises an enzyme sequence between the signal sequence and the desired protein sequence.

    Abstract translation: 本发明提供了改进蛋白质生产的方法和组合物。 该方法包括以下步骤：（a）向宿主细胞中引入包含可操作地连接到所需蛋白质序列的信号序列的第一核酸序列; （b）表达第一核酸序列; （c）共表达编码选自bip1，ero1，pdi1，tig1，prp1，ppi1，ppi2，prp3，prp4，calnexin和Ihs1的伴侣或折叠酶的第二核酸序列; 和（d）收集从宿主细胞分泌的所需蛋白质。 第一核酸序列任选地包含信号序列和所需蛋白质序列之间的酶序列。

    NEUTRAL CELLULASE CATALYTIC CORE AND METHOD OF PRODUCING SAME
    10.
    发明申请
    NEUTRAL CELLULASE CATALYTIC CORE AND METHOD OF PRODUCING SAME 审中-公开
    中性纤维素酶催化核及其生产方法

    公开(公告)号：WO2006071598A1

    公开(公告)日：2006-07-06

    申请号：PCT/US2005/045859

    申请日：2005-12-16

    Applicant: GENENCOR INTERNATIONAL, INC. ,  BAO, Kai ,  WANG, Huaming

    Inventor： BAO, Kai ,  WANG, Huaming

    IPC: C12N9/42 ,  C11D3/386 ,  D06M16/00 ,  D21C5/00 ,  A23K1/165 ,  C12N15/55

    CPC classification number: C12N9/2437 ,  A23K10/14 ,  A23K20/189 ,  C11D3/38645 ,  D06M16/003 ,  D06M2101/06 ,  D06M2200/50 ,  D06P1/38 ,  D21C5/005

    Abstract: The present invention relates to the cloning and high level expression of novel cellulase proteins or derivatives thereof in the in a host cell. Further aspects of the present invention relate to transformants that express the novel cellulases, and expression vectors comprising the DNA gene fragments or variants thereof that code for the novel cellulases derived from Actinomycete using genetic engineering techniques. The present invention is also directed to novel cellulase compositions and methods of use therefore in industrial processes. In particular, the present invention is related to treating textiles with a novel cellulase derived from Actinomycete spp . The present invention also relates to the use of cellulase derived from Actinomycete spp . to enhance the digestibility of animal feed, in detergents, in the treatment of pulp and paper and in the production of starch and treatment of by-products thereof.

    Abstract translation: 本发明涉及在宿主细胞中克隆和高水平表达新的纤维素酶蛋白或其衍生物。 本发明的其它方面涉及表达新型纤维素酶的转化体，以及包含使用遗传工程技术编码来自放线菌的新型纤维素酶的DNA基因片段或其变体的表达载体。 本发明还涉及新型纤维素酶组合物和因此在工业过程中使用的方法。 特别地，本发明涉及用衍生自放线菌属的新型纤维素酶处理纺织品。 本发明还涉及来自放线菌属的纤维素酶的用途。 以提高动物饲料，洗涤剂，纸浆和纸张的处理和淀粉生产的消化率及其副产物的处理。

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