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    METHODS AND SYSTEMS FOR PURE DYE INSTRUMENT NORMALIZATION
    1.
    发明申请
    METHODS AND SYSTEMS FOR PURE DYE INSTRUMENT NORMALIZATION 审中-公开
    纯正仪器仪表的方法和系统

    公开(公告)号：WO2016127090A1

    公开(公告)日：2016-08-11

    申请号：PCT/US2016/016832

    申请日：2016-02-05

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： MARKS, Jeffrey

    IPC: G01N21/27 ,  G01N21/64

    CPC classification number: C12Q1/686 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2021/6421 ,  G01N2201/04 ,  G01N2201/068 ,  G01N2201/12746

    Abstract: The present teachings relate to a method and system for normalizing spectra across multiple instruments. The method (800) comprises at least one reference instrument and a test instrument. Each instrument comprises at least one excitation filter and at least one emission filter arranged in pairs. Each instrument further comprises a pure dye plate comprising a plurality of wells. Each well contains a plurality of dyes where each dye comprises a fluorescent component. Fluorescent spectra are obtained from each instrument (805, 820) for each dye across multiple filter combinations to contribute to a pure dye matrix Mref for the reference instrument and pure dye matrix M for the test instrument. The pure dye spectra can then be multiplied by correction factors (840) for each filter pair to result in corrected pure dye spectra, then normalized (845) and the multicomponenting data can be extracted (850).

    Abstract translation: 本教导涉及用于在多个仪器上归一化光谱的方法和系统。 方法（800）包括至少一个参考仪器和测试仪器。 每个仪器包括至少一个激励滤波器和成对布置的至少一个发射滤波器。 每个仪器还包括包含多个孔的纯染料板。 每个孔含有多种染料，其中每种染料包含荧光组分。 对于每种染料，通过多个过滤器组合从每个仪器（805,820）获得荧光光谱，以有助于参考仪器的纯染料矩阵Mref和测试仪器的纯染料矩阵M. 然后可以将纯染料光谱乘以每个过滤器对的校正因子（840），以产生校正的纯染料光谱，然后归一化（845），并且可以提取多组分数据（850）。

    OPTICAL SYSTEMS AND METHODS FOR BIOLOGICAL ANALYSIS
    2.
    发明申请
    OPTICAL SYSTEMS AND METHODS FOR BIOLOGICAL ANALYSIS 审中-公开
    光学系统和生物分析方法

    公开(公告)号：WO2014130970A2

    公开(公告)日：2014-08-28

    申请号：PCT/US2014/018110

    申请日：2014-02-24

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： CHEN, Mingsong ,  AGUANNO, Mauro ,  BOO, Kuan Moon ,  SOH, Woon Liang Terence ,  FREUDENTHAL, Jacob ,  MARKS, Jeffrey

    IPC: G01N21/27

    CPC classification number: G01N21/6486 ,  G01N21/0332 ,  G01N21/274 ,  G01N21/645 ,  G01N21/6452 ,  G01N2201/061 ,  G01N2201/0624 ,  G01N2201/0627 ,  G01N2201/121

    Abstract: An instrument for processing and/or measuring a biological process contains a sample processing system, an excitation source, an excitation optical system, an optical sensor, and an emission optical system. The sample processing system is configured to retain a first sample holder and a second sample holder, wherein the number of sample cells is different for each sample holder or a characteristic dimension for the first sample cells is different from that of the second sample holder. The instrument also includes an excitation source temperature controller comprising a temperature sensor that is coupled to the excitation source. The temperature controller is configured to produce a first target temperature when the first sample holder is retained by the instrument and to produce a second target temperature when the second sample holder is retained by the instrument.

    Abstract translation: 用于处理和/或测量生物过程的仪器包括样品处理系统，激发源，激发光学系统，光学传感器和发射光学系统。 样品处理系统被配置为保持第一样品保持器和第二样品保持器，其中样品池的数量对于每个样品保持器是不同的，或者第一样品池的特征尺寸与第二样品保持器的特征尺寸不同。 仪器还包括激发源温度控制器，其包括耦合到激发源的温度传感器。 温度控制器被配置为当第一样品保持器被仪器保持时产生第一目标温度，并且当第二样品保持器被仪器保持时产生第二目标温度。

    METHODS AND SYSTEMS FOR A DIGITAL PCR EXPERIMENT DESIGNER
    3.
    发明申请
    METHODS AND SYSTEMS FOR A DIGITAL PCR EXPERIMENT DESIGNER 审中-公开
    数字PCR实验设计者的方法和系统

    公开(公告)号：WO2014043581A1

    公开(公告)日：2014-03-20

    申请号：PCT/US2013/059815

    申请日：2013-09-13

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： MAJUMDAR, Nivedita Sumi ,  WESSEL, Thomas ,  WOO, David C. ,  SIKORA, Marcin ,  JANAWAY, Gordon ,  RAIZADA, Shweta ,  LANKESTER, Joanna ,  MARKS, Jeffrey ,  WESSEL, Daniel

    IPC: G06F19/24

    CPC classification number: G06F19/20 ,  C12Q1/686 ,  G06F19/24

    Abstract: A computer-implemented method for designing a digital PCR (dPCR) experiment is provided. The method includes receiving, from a user, a selection of optimization type. The optimization type may be maximizing the dynamic range, minimizing the number of substrates including reaction sites needed for the experiment, determining a dilution factor, or determining the lower limit of detection, for example. The method further includes receiving, from the user, a precision measure for an experiment, and a minimum concentration of a target in a reaction site for the experiment. The method also includes determining a set of dPCR experiment design factors for the experiment based on the optimization type. The set of dPCR experiment design factors is then displayed to the user.

    Abstract translation: 提供了一种用于设计数字PCR（dPCR）实验的计算机实现方法。 该方法包括从用户接收优化类型的选择。 优化类型可以使动态范围最大化，例如最小化包括实验所需的反应位点，确定稀释因子或确定检测下限的底物数量。 该方法还包括从用户接收实验的精确度量，以及用于实验的反应部位中靶的最小浓度。 该方法还包括基于优化类型确定实验的一组dPCR实验设计因子。 然后将该组dPCR实验设计因子显示给用户。

    SYSTEM AND METHODS FOR CALIBRATING BINDING DYES
    5.
    发明申请
    SYSTEM AND METHODS FOR CALIBRATING BINDING DYES 审中-公开
    用于校准结合染色体的系统和方法

    公开(公告)号：WO2016127080A1

    公开(公告)日：2016-08-11

    申请号：PCT/US2016/016817

    申请日：2016-02-05

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： CHU, Yong ,  FREUDENTHAL, Jacob ,  MARKS, Jeffrey

    IPC: G01N21/27 ,  G01N21/64 ,  C12Q1/68

    CPC classification number: C12Q1/686 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2201/127

    Abstract: A computer-implemented method (500) for calibrating a fluorescent dye is described. The method (500) comprises imaging a sample holder, loaded into an instrument, at more than one channel. The sample holder comprises a plurality of reaction sites and more than one dye type, with each dye occupying more than one reaction site. The method further comprises identifying a peak channel for each dye on the sample holder (508), normalizing each channel to the peak channel for each dye (510), and producing a dye matrix (518) that comprises a set of dye reference values.

    Abstract translation: 描述了用于校准荧光染料的计算机实现的方法（500）。 方法（500）包括在多个通道上成像加载到仪器中的样品保持器。 样品架包括多个反应位点和多于一种染料类型，每种染料占据多于一个反应位点。 该方法还包括鉴定样品保持器（508）上的每种染料的峰值通道，将每个通道标准化为每个染料（510）的峰值通道，以及产生包含一组染料参考值的染料基质（518）。

    METHODS AND SYSTEMS FOR ANALYZING BIOLOGICAL REACTION SYSTEMS
    6.
    发明申请
    METHODS AND SYSTEMS FOR ANALYZING BIOLOGICAL REACTION SYSTEMS 审中-公开
    分析生物反应系统的方法和系统

    公开(公告)号：WO2014153369A1

    公开(公告)日：2014-09-25

    申请号：PCT/US2014/031137

    申请日：2014-03-18

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： CHU, Yong ,  MARKS, Jeffrey

    IPC: C12Q1/68 ,  G06K9/00 ,  G06T5/00

    CPC classification number: G06T7/74 ,  C12Q1/686 ,  G06K9/00 ,  G06K2209/07 ,  G06T5/002 ,  G06T7/0012 ,  G06T7/11 ,  G06T7/136 ,  G06T7/194 ,  G06T2207/10064 ,  G06T2207/20024 ,  G06T2207/20208 ,  G06T2207/30072

    Abstract: A method for analyzing biological reaction systems is provided. The method includes receiving an image of a substrate including a plurality of reaction sites after a biological reaction has taken place. Next, the method includes removing a noise background from the first image. The method includes determining an initial position of each reaction site based on an intensity threshold to generate a initial position set, then refining the initial position set of each reaction site based on an expected pattern of locations of the plurality of reaction sites to generate a first refined position set. The method further includes determining a presence or absence of a fluorescent emission from each reaction site based on the first refined position set and the first image.

    Abstract translation: 提供了分析生物反应体系的方法。 该方法包括在发生生物反应之后接收包括多个反应位点的底物的图像。 接下来，该方法包括从第一图像中去除噪声背景。 该方法包括基于强度阈值确定每个反应位点的初始位置以产生初始位置集合，然后基于多个反应位点的预期位置模式来精化每个反应位点的初始位置集合，以产生第一 精致的位置集。 该方法还包括基于第一精制位置集和第一图像来确定来自每个反应位点的荧光发射的存在或不存在。

    METHODS AND SYSTEMS FOR BIOLOGICAL INSTRUMENT CALIBRATION
    9.
    发明申请
    METHODS AND SYSTEMS FOR BIOLOGICAL INSTRUMENT CALIBRATION 审中-公开
    生物仪器校准的方法和系统

    公开(公告)号：WO2016127124A2

    公开(公告)日：2016-08-11

    申请号：PCT/US2016/016882

    申请日：2016-02-05

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： CHU, Yong ,  MARKS, Jeffrey ,  FREUDENTHAL, Jacob ,  WESSEL, Thomas ,  WOO, David

    IPC: G01N21/27 ,  G01N21/64

    CPC classification number: G01N21/6428 ,  C12Q1/6851 ,  C12Q1/686 ,  G01N21/274 ,  G01N21/278 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2021/6439 ,  G01N2021/6471 ,  G01N2201/127 ,  G01N2201/13 ,  G01N2333/922

    Abstract: In one exemplary embodiment, a method for calibrating an instrument is provided. The instrument includes an optical system capable of imaging florescence emission from a plurality of reaction sites. The method includes performing a region-of-interest (ROI) calibration to determine reaction site positions in an image. The method further includes performing a pure dye calibration to determine the contribution of a fluorescent dye used in each reaction site by comparing a raw spectrum of the fluorescent dye to a pure spectrum calibration data of the fluorescent dye. The method further includes performing an instrument normalization calibration to determine a filter normalization factor. The method includes performing an RNase P validation to validate the instrument is capable of distinguishing between two different quantities of sample.

    Abstract translation: 在一个示例性实施例中，提供了一种用于校准仪器的方法。 该仪器包括能够成像来自多个反应位点的荧光发射的光学系统。 该方法包括执行感兴趣区域（ROI）校准以确定图像中的反应位置位置。 该方法还包括进行纯染料校准，以通过将荧光染料的原始光谱与荧光染料的纯光谱校准数据进行比较来确定每个反应位点中使用的荧光染料的贡献。 该方法还包括执行仪器归一化校准以确定滤波器归一化因子。 该方法包括执行RNase P验证以验证仪器能够区分两种不同数量的样品。

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