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公开(公告)号：WO2007030811A2
公开(公告)日：2007-03-15
申请号：PCT/US2006/035243
申请日：2006-09-11
Applicant: DUKE UNIVERSITY , GUILAK, Farshid , ESTES, Bradley, T. , WU, Arthur, W.
Inventor： GUILAK, Farshid , ESTES, Bradley, T. , WU, Arthur, W.
IPC: A61K35/12
CPC classification number: A61L27/54 , A61K35/12 , A61K35/28 , A61K35/32 , A61K35/33 , A61K38/1841 , A61K38/1875 , A61K38/30 , C12N5/0667 , C12N2501/105 , C12N2501/11 , C12N2501/115 , C12N2501/135 , C12N2501/15 , C12N2501/155 , C12N2501/23 , C12N2501/37 , C12N2501/39 , C12N2533/30 , C12N2533/40
Abstract: The presently disclosed subject matter generally relates to methods and systems for facilitating the growth and differentiation of adipose-derived stem cells for laboratory and therapeutic applications. The cells can be employed alone or in conjunction with unique biologically-compatible scaffold structures to generate differentiated tissues and structures, both in vitro and in vivo. The presently disclosed subject matter further relates to methods of forming and using improved tissue engineered scaffolds that can be used as substrates to facilitate the growth and differentiation of cells.
Abstract translation: 目前公开的主题一般涉及促进脂肪来源的干细胞在实验室和治疗应用中的生长和分化的方法和系统。 细胞可以单独使用或与独特的生物相容性支架结构结合使用，以在体外和体内产生分化的组织和结构。 目前公开的主题还涉及形成和使用改进的组织工程化支架的方法，其可以用作底物以促进细胞的生长和分化。
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公开(公告)号：WO2006052991A2
公开(公告)日：2006-05-18
申请号：PCT/US2005/040477
申请日：2005-11-08
Applicant: THE GENERAL HOSPTIAL CORPORATION , SCADDEN, David, T. , ADAMS, Gregor , KORNENBERG, Henry , CALVI, Laura, M.
Inventor： SCADDEN, David, T. , ADAMS, Gregor , KORNENBERG, Henry , CALVI, Laura, M.
IPC: A61K39/395 , A61K35/14
CPC classification number: A61K38/29 , C12N5/0647 , C12N2501/37 , C12N2502/1394
Abstract: The invention relates to methods for manipulating hematopoietic stem or progenitor cells, mesenchymal stem cells, epithelial stem cells, neural stem cells and related products through activation of the PTH/PTHrP receptor in neighboring cells.
Abstract translation: 本发明涉及通过激活邻近细胞中的PTH / PTHrP受体来操纵造血干细胞或祖细胞，间充质干细胞，上皮干细胞，神经干细胞和相关产物的方法。
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公开(公告)号：WO2007003958A3
公开(公告)日：2007-04-05
申请号：PCT/GB2006002521
申请日：2006-07-06
Applicant: UNIV BRISTOL , HOLLANDER ANTHONY PETER , KAFIENAH WA EL ZAKI
Inventor： HOLLANDER ANTHONY PETER , KAFIENAH WA EL ZAKI
CPC classification number: A61L27/3852 , A61K38/29 , A61L27/3817 , A61L27/3834 , A61L2430/06 , C12N5/0655 , C12N2501/37 , C12N2503/02
Abstract: The invention relates to the use of parathyroid hormone-related protein (PTHrP) in the prevention of hypertrophy in chondrogenic cells for cartilage replacement. A method for engineering three dimensional cartilage constructs from chondrogenic cells is provided, said method comprising a step of treating the chondrogenic cells or immature constructs with PTHrP to regulate hypertrophy. Also provided are: three dimensional cartilage produced by the method of the invention, and an engineered cartilage construct comprising chondrogenic cells and a bioactive scaffold capable of controlled release of PTHrP. In addition, the invention provides a method for the treatment of osteoarthritis.
Abstract translation: 本发明涉及甲状旁腺激素相关蛋白（PTHrP）在预防用于软骨替代的成软骨细胞中的肥大中的用途。 提供了用于从软骨形成细胞改造三维软骨构建体的方法，所述方法包括用PTHrP处理软骨形成细胞或未成熟构建体以调节肥大的步骤。 还提供了：通过本发明的方法产生的三维软骨以及包含软骨细胞和能够控制释放PTHrP的生物活性支架的工程化软骨构建体。 另外，本发明提供了用于治疗骨关节炎的方法。
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公开(公告)号：WO03102171A9
公开(公告)日：2005-01-20
申请号：PCT/US0316096
申请日：2003-05-22
Applicant: BECTON DICKINSON CO , SCHARP DAVID W , ROBBINS NEIL , JURGENSEN STEWART , COUTTS MARGARET , LATTA PAUL P , MCINTYRE CATHERINE , PRESNELL SHARON C , HEIDARAN MOHAMMAD , HAALAND PERRY
Inventor： SCHARP DAVID W , ROBBINS NEIL , JURGENSEN STEWART , COUTTS MARGARET , LATTA PAUL P , MCINTYRE CATHERINE , PRESNELL SHARON C , HEIDARAN MOHAMMAD , HAALAND PERRY
CPC classification number: C12N5/0676 , A61K35/12 , A61K2035/126 , C12N5/0037 , C12N2500/25 , C12N2500/38 , C12N2500/46 , C12N2501/01 , C12N2501/105 , C12N2501/11 , C12N2501/113 , C12N2501/115 , C12N2501/117 , C12N2501/12 , C12N2501/135 , C12N2501/15 , C12N2501/16 , C12N2501/165 , C12N2501/235 , C12N2501/315 , C12N2501/335 , C12N2501/34 , C12N2501/345 , C12N2501/35 , C12N2501/37 , C12N2501/39 , C12N2501/392 , C12N2501/41 , C12N2501/83 , C12N2501/85 , C12N2501/998 , C12N2506/22
Abstract: This invention relates, e.g., to a method for expanding mammalian acinar cells, comprising culturing the cells in a cell culture system comprising a cell culture medium and a cell attachment surface, under conditions wherein the acinar cells undergo a 3-4 fold expansion together with transdifferentiation into a modified cell phenotype (IP cells) showing characteristics of acinar cells and liver cells. The invention also relates to a method for transforming these IP cells to insulin-producing cells in vitro, comprising culturing the cells in a novel, defined medium. Also disclosed are suitable culture media for performing these methods, isolated cells having the phenotype of IP cells and/or produced by these methods, and kits for performing the methods.
Abstract translation: 本发明涉及例如扩展哺乳动物腺泡细胞的方法，包括在包含细胞培养基和细胞附着表面的细胞培养系统中培养细胞，其中腺泡细胞与 转化为显示腺泡细胞和肝细胞特征的修饰的细胞表型（IP细胞）。 本发明还涉及用于在体外将这些IP细胞转化成胰岛素产生细胞的方法，包括在新颖的限定培养基中培养细胞。 还公开了用于进行这些方法的合适的培养基，具有IP细胞表型和/或通过这些方法产生的分离的细胞，以及用于进行该方法的试剂盒。
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公开(公告)号：WO2003102171A1
公开(公告)日：2003-12-11
申请号：PCT/US2003/016096
申请日：2003-05-22
Applicant: BECTON, DICKINSON AND COMPANY , SCHARP, David, W. , ROBBINS, Neil , JURGENSEN, Stewart , COUTTS, Margaret , LATTA, Paul, P. , MCINTYRE, Catherine , PRESNELL, Sharon, C. , HEIDARAN, Mohammad , HAALAND, Perry
Inventor： SCHARP, David, W. , ROBBINS, Neil , JURGENSEN, Stewart , COUTTS, Margaret , LATTA, Paul, P. , MCINTYRE, Catherine , PRESNELL, Sharon, C. , HEIDARAN, Mohammad , HAALAND, Perry
IPC: C12N5/00
CPC classification number: C12N5/0676 , A61K35/12 , A61K2035/126 , C12N5/0037 , C12N2500/25 , C12N2500/38 , C12N2500/46 , C12N2501/01 , C12N2501/105 , C12N2501/11 , C12N2501/113 , C12N2501/115 , C12N2501/117 , C12N2501/12 , C12N2501/135 , C12N2501/15 , C12N2501/16 , C12N2501/165 , C12N2501/235 , C12N2501/315 , C12N2501/335 , C12N2501/34 , C12N2501/345 , C12N2501/35 , C12N2501/37 , C12N2501/39 , C12N2501/392 , C12N2501/41 , C12N2501/83 , C12N2501/85 , C12N2501/998 , C12N2506/22 , Y02A50/471
Abstract: This invention relates, e.g., to a method for expanding mammalian acinar cells, comprising culturing the cells in a cell culture system comprising a cell culture medium and a cell attachment surface, under conditions wherein the acinar cells undergo a 3-4 fold expansion together with transdifferentiation into a modified cell phenotype (IP cells) showing characteristics of acinar cells and liver cells. The invention also relates to a method for transforming these IP cells to insulin-producing cells in vitro , comprising culturing the cells in a novel, defined medium. Also disclosed are suitable culture media for performing these methods, isolated cells having the phenotype of IP cells and/or produced by these methods, and kits for performing the methods.
Abstract translation: 本发明涉及例如扩展哺乳动物腺泡细胞的方法，包括在包括细胞培养基和细胞附着表面的细胞培养系统中培养细胞，其中腺泡细胞与 转化为显示腺泡细胞和肝细胞特征的修饰的细胞表型（IP细胞）。 本发明还涉及在体外将这些IP细胞转化成胰岛素生成细胞的方法，包括在新颖的限定培养基中培养细胞。 还公开了用于进行这些方法的合适的培养基，具有IP细胞表型和/或通过这些方法产生的分离的细胞，以及用于进行该方法的试剂盒。
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Patent Agency Ranking6.发明申请
公开(公告)号：WO9927977A3
公开(公告)日：1999-09-10
申请号：PCT/EP9807708
申请日：1998-11-30
Applicant: BIOTECHNOLOG FORSCHUNG GMBH , MAYER HUBERT , GROSS GERHARD , ROHDE MANFRED , JAEGER VOLKER , SCHLIEPHAKE HENNING , BARTHOLD MARC
Inventor： MAYER HUBERT , GROSS GERHARD , ROHDE MANFRED , JAEGER VOLKER , SCHLIEPHAKE HENNING , BARTHOLD MARC
CPC classification number: A61L27/3821 , A61K35/12 , A61L27/3608 , A61L27/3683 , A61L2430/02 , C12N5/0654 , C12N2501/11 , C12N2501/115 , C12N2501/155 , C12N2501/165 , C12N2501/37
Abstract: The invention relates to a bone matrix which can be obtained by: (a) starting out from an explantation and obtaining osteogenic precursor cells or starting out from an osteogenic cell line; (b) optionally expanding the obtained osteogenic precursor cells or the osteogenic cell line in-vitro; (c) permitting the osteogenic precursor cells to propagate, said cells being obtained according to (a) or expanded according to (b), or permitting the cell line to propagate, said cell line being inserted according to (a) or expanded according to (b), and optionally obtaining osteogenic cells; (d) separate from step (c), permitting the osteogenic cells to synthesize in-vitro in an extracellular matrix, said cells being obtained according to (a) or expanded according to (b), or permitting the cell line to synthesize in-vitro in an extracellular matrix, said cell line being introduced according to (a) or expanded according to (b); (e) optionally liberating the synthesized matrix from cells and optionally obtaining a cell-free bone matrix; (f) populating the obtained cell-free bone matrix once more with osteogenic cells which have been obtained according to (c) and optionally obtaining a populated bone matrix by using osteogenic cells.
Abstract translation: 本发明涉及一种骨基质，这是在该得到的原料（a）一种外植体，和成骨祖细胞的获得或从成骨细胞系开始，（b）任选扩大衍生的成骨前体细胞或体外成骨细胞系，（c）中 根据（a）或根据（b）中展开的成骨前体细胞或根据（a）中，根据（a）中回收的用过的或根据（b）中可以多重扩增的细胞系和任选地，成骨细胞获胜，（d）中分别从测量（c）中得到 可以合成或根据（b）中展开的成骨细胞或根据（a）中使用，或根据（b）中的体外细胞外基质扩增的细胞系，（e）中合成的基质，在适当情况下，释放的细胞和任选的无细胞的骨基质的胜，（f）中，将回收的 再次根据（c）中已产生结算与骨原细胞的无细胞的骨基质，并gegebenenf 承滴盘由骨细胞的骨基质胜填充。
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公开(公告)号：WO2014015109A1
公开(公告)日：2014-01-23
申请号：PCT/US2013/051022
申请日：2013-07-18
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： KUO, Alfred
CPC classification number: C12N5/0655 , A61K35/00 , A61K35/32 , C12N2500/90 , C12N2501/15 , C12N2501/155 , C12N2501/37
Abstract: The present disclosure provides methods of producing cartilage in vitro . The present disclosure provides treatment methods, involving introducing in vitro -produced cartilage into a treatment site in vivo . The present disclosure provides methods of enhancing bone formation, the method involving introducing in vitro -produced hypertrophic cartilage into a treatment site in vivo .
Abstract translation: 本公开提供了在体外生产软骨的方法。 本公开提供了治疗方法，包括将体外产生的软骨引入体内的治疗部位。 本公开提供了增强骨形成的方法，该方法包括将体外产生的肥大软骨引入体内的治疗部位。
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公开(公告)号：WO2008156725A3
公开(公告)日：2009-04-23
申请号：PCT/US2008007485
申请日：2008-06-16
Applicant: UNIV COLUMBIA , LU HELEN , JIANG JIE
CPC classification number: A61K38/29 , A61K35/12 , A61L27/227 , A61L27/3612 , A61L27/3654 , A61L27/3817 , A61L27/3852 , A61L2430/06 , C12N5/0655 , C12N2501/37 , C12N2501/395 , C12N2533/12 , C12N2533/40
Abstract: Methods and apparatuses are provided for inhibiting articular cartilage mineralization in a subject comprising administering to articular cartilage of the subject an amount of a functional domain of parathyroid hormone- related peptide (PTHrP). In addition, methods and apparatuses for preventing or treating osteoarthritis in a subject comprising administering to articular cartilage of the subject a prophylactically or therapeutically effective amount of a functional domain of parathyroid hormone- related peptide (PTHrP), respectively, are provided. Also, methods and apparatuses for promoting articular cartilage repair in a subject comprising comprising administering to articular cartilage of the subject an effective amount functional domain of parathyroid hormone-related peptide (PTHrP) are provided. Further, methods for diminishing damaging effects of physical activity on a joint in a subject comprising administering to articular cartilage of the subject an amount of a functional domain of parathyroid hormone-related peptide (PTHrP) are provided.
Abstract translation: 提供了用于抑制受试者的关节软骨矿化的方法和装置，包括向受试者的关节软骨施用一定量的甲状旁腺激素相关肽（PTHrP）的功能结构域。 此外，提供了用于预防或治疗受试者骨关节炎的方法和装置，其包括分别对受试者的关节软骨施用预防或治疗有效量的甲状旁腺激素相关肽（PTHrP）的功能结构域。 此外，提供了一种用于促进受试者中的关节软骨修复的方法和装置，其包括对所述受试者的关节软骨施用有效量的甲状旁腺激素相关肽（PTHrP）的功能结构域。 此外，提供减少身体活动对受试者关节的损伤作用的方法，包括给予受试者的关节软骨一定量的甲状旁腺激素相关肽（PTHrP）的功能结构域。
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公开(公告)号：WO2007028101A9
公开(公告)日：2007-05-24
申请号：PCT/US2006034374
申请日：2006-09-05
Applicant: UNIV CALIFORNIA , PARHAMI FARHAD
Inventor： PARHAMI FARHAD
CPC classification number: A61K31/575 , A61K31/00 , A61K31/202 , A61K31/557 , A61K31/57 , A61K31/59 , A61K31/663 , A61K38/1841 , A61K38/1875 , A61K38/23 , A61K38/29 , A61K38/30 , C12N5/0654 , C12N2501/02 , C12N2501/105 , C12N2501/15 , C12N2501/155 , C12N2501/37 , C12N2501/39 , C12N2501/415 , C12N2501/999 , A61K2300/00
Abstract: The present invention discloses osteogenic and anti-adipogenic oxysterols. Agents and methods for protecting, blocking or rescuing marrow stromal cells from the inhibitory effects of oxidative stress on their osteoblastic cellular differentiation are disclosed. Exemplary agents include oxysterols alone or in synergistic combinations, as well as hedgehog or Wnt signaling activators. The synergistic effects of oxysterols and bone morphogenic proteins are also disclosed.
Abstract translation: 本发明公开了成骨和抗脂肪氧化固醇。 公开了用于保护，阻断或拯救骨髓基质细胞免受氧化应激对其成骨细胞分化的抑制作用的药剂和方法。 示例性药剂包括单独的氧固醇或协同组合，以及刺猬蛋白或Wnt信号传导激活剂。 还公开了氧固醇和骨形态发生蛋白的协同作用。
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10.发明申请
公开(公告)号：WO03102134A2
公开(公告)日：2003-12-11
申请号：PCT/US0316124
申请日：2003-05-22
Applicant: BECTON DICKINSON CO , NOVOCELL INC , PRESNELL SHARON C , ROBBINS NEIL , HEIDARAN MOHAMMAD , SPARGO CATHERINE A , HAALAND PERRY , JURGENSEN STEWART , LATTA PAUL P , SCHARP DAVID W , COUTTS MARGARET
Inventor： PRESNELL SHARON C , ROBBINS NEIL , HEIDARAN MOHAMMAD , SPARGO CATHERINE A , HAALAND PERRY , JURGENSEN STEWART , LATTA PAUL P , SCHARP DAVID W , COUTTS MARGARET
CPC classification number: C12N5/0676 , A61K35/12 , A61K2035/126 , C12N5/0037 , C12N2500/25 , C12N2500/38 , C12N2500/46 , C12N2501/01 , C12N2501/105 , C12N2501/11 , C12N2501/113 , C12N2501/115 , C12N2501/117 , C12N2501/12 , C12N2501/135 , C12N2501/15 , C12N2501/16 , C12N2501/165 , C12N2501/235 , C12N2501/315 , C12N2501/335 , C12N2501/34 , C12N2501/345 , C12N2501/35 , C12N2501/37 , C12N2501/39 , C12N2501/392 , C12N2501/41 , C12N2501/83 , C12N2501/85 , C12N2501/998 , C12N2506/22
Abstract: This invention relates, e.g., to a method for expanding mammalian acinar cells, comprising culturing the cells in a cell culture system comprising a cell culture medium and a cell attachment surface, under conditions wherein the acinar cells undergo a 3-4 fold expansion together with transdifferentiation into a modified cell phenotype (IP cells) showing characteristics of acinar cells and liver cells. The invention also relates to a method for transforming these IP cells to insulin-producing cells in vitro, comprising culturing the cells in a novel, defined medium. Also disclosed are suitable culture media for performing these methods, isolated cells having the phenotype of IP cells and/or produced by these methods, and kits for performing the methods.
Abstract translation: 本发明涉及例如扩展哺乳动物腺泡细胞的方法，包括在包括细胞培养基和细胞附着表面的细胞培养系统中培养细胞，其中腺泡细胞与 转化为显示腺泡细胞和肝细胞特征的修饰的细胞表型（IP细胞）。 本发明还涉及在体外将这些IP细胞转化成胰岛素生成细胞的方法，包括在新颖的限定培养基中培养细胞。 还公开了用于进行这些方法的合适的培养基，具有IP细胞表型和/或通过这些方法产生的分离的细胞，以及用于进行该方法的试剂盒。
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