公开(公告)号：WO2014076571A8

公开(公告)日：2014-07-10

申请号：PCT/IB2013002974

申请日：2013-11-18

Applicant: CELLECTIS ,  TOTAL MARKETING SERVICES

Inventor： DUCHATEAU PHILIPPE ,  DABOUSSI FAYZA

IPC: C12N15/82

CPC classification number: C12N15/8213 ,  C07K14/195 ,  C07K2319/80 ,  C12N9/1241 ,  C12N9/22 ,  C12N15/52 ,  C12R1/89 ,  C12Y207/07009 ,  C12Y301/00 ,  C12Y301/11002

Abstract: The invention relates to a method for modifying genetic material in algal cells that includes the use of rare-cutting endonuclease to target specific genomic sequences. In particular, the invention relates to a method for modifying genetic material in algal cells wherein rare-cutting endonuclease, especially a homing endonuclease or a TALE-Nuclease, is expressed over several generations to efficiently modify said target genome sequences.

公开(公告)号：WO2014076571A3

公开(公告)日：2014-11-06

申请号：PCT/IB2013002974

申请日：2013-11-18

Applicant: CELLECTIS ,  TOTAL MARKETING SERVICES

Inventor： DUCHATEAU PHILIPPE ,  DABOUSSI FAYZA

IPC: C12N15/82 ,  C12N9/22

CPC classification number: C12N15/8213 ,  C07K14/195 ,  C07K2319/80 ,  C12N9/1241 ,  C12N9/22 ,  C12N15/52 ,  C12R1/89 ,  C12Y207/07009 ,  C12Y301/00 ,  C12Y301/11002

Abstract: The invention relates to a method for modifying genetic material in algal cells that includes the use of rare-cutting endonuclease to target specific genomic sequences. In particular, the invention relates to a method for modifying genetic material in algal cells wherein rare-cutting endonuclease, especially a homing endonuclease or a TALE-Nuclease, is expressed over several generations to efficiently modify said target genome sequences.

Abstract translation: 本发明涉及一种在藻类细胞中修饰遗传物质的方法，包括使用稀有切割内切核酸酶靶向特定基因组序列。 特别地，本发明涉及用于修饰藻类细胞中的遗传物质的方法，其中稀释切割内切核酸酶，特别是归巢内切核酸酶或TALE-核酸酶在几代中表达以有效修饰所述靶基因组序列。

公开(公告)号：WO2019060672A1

公开(公告)日：2019-03-28

申请号：PCT/US2018/052148

申请日：2018-09-21

Applicant: MODERN MEADOW, INC.

Inventor： DAI, Lixin ,  SRIVASTAVA, Poonam

IPC: C07K14/78 ,  C14C13/00 ,  C12P21/00 ,  C07K14/435

CPC classification number: C07K14/78 ,  C07K14/195 ,  C07K14/245 ,  C12N9/0006 ,  C12N9/001 ,  C12N9/0071 ,  C12N9/1205 ,  C12N9/1241 ,  C12N9/18 ,  C12N9/90 ,  C12N15/815 ,  C12R1/84 ,  C12Y101/01019 ,  C12Y101/0102 ,  C12Y101/01022 ,  C12Y103/03012 ,  C12Y114/11004 ,  C12Y207/01001 ,  C12Y207/01043 ,  C12Y207/07009 ,  C12Y207/07044 ,  C12Y207/07069 ,  C12Y301/01017 ,  C12Y301/01019 ,  C12Y301/03025 ,  C12Y501/03018 ,  C12Y504/02002 ,  D01F1/02 ,  D01F4/00

Abstract: This invention relates to genetically engineered strains of yeast and methods for producing recombinant protein (e.g., collagen). Recombinant protein of the present invention is used to produce biofabricated leather or a material having leather-like properties containing recombinant or engineered collagen. The yeast strains are engineered to produce ascorbate and/or increased production of α ketoglutarate.

公开(公告)号：WO2014207043A1

公开(公告)日：2014-12-31

申请号：PCT/EP2014/063393

申请日：2014-06-25

Applicant: CELLECTIS

Inventor： DUCHATEAU, Philippe ,  DABOUSSI, Fayza ,  SOURDIVE, David ,  EPINAT, Jean-Charles

IPC: C12N1/13 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10 ,  C12N9/12 ,  C12N9/16 ,  C12P7/64 ,  C12N15/79 ,  C12R1/89

CPC classification number: C12N15/8247 ,  C12N9/0006 ,  C12N9/001 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/1241 ,  C12N9/16 ,  C12N15/79 ,  C12P7/64 ,  C12P7/6427 ,  C12Y101/01008 ,  C12Y103/01009 ,  C12Y114/19006 ,  C12Y203/01 ,  C12Y207/07009 ,  C12Y301/02022

Abstract: The invention provides engineered diatoms and methods of producing oil using diatoms. The invention also provides methods of modifying the lipids quantity and/or quality produced by diatom organisms through genome engineering. Also provided are oils, fuels, oleochemicals, chemical precursors, and other compounds manufactured from such modified diatoms.

Abstract translation: 本发明提供工程硅藻和使用硅藻生产油的方法。 本发明还提供了通过基因组工程改变由硅藻生物产生的脂质量和/或质量的方法。 还提供油​​，燃料，油化学品，化学前体和由这种改性的硅藻制造的其它化合物。

公开(公告)号：WO2014076571A2

公开(公告)日：2014-05-22

申请号：PCT/IB2013002974

申请日：2013-11-18

Applicant: CELLECTIS ,  TOTAL MARKETING SRRVICES

Inventor： DUCHATEAU PHILIPPE ,  DABOUSSI FAYZA

IPC: C12N15/82

CPC classification number: C12N15/8213 ,  C07K14/195 ,  C07K2319/80 ,  C12N9/1241 ,  C12N9/22 ,  C12N15/52 ,  C12R1/89 ,  C12Y207/07009 ,  C12Y301/00 ,  C12Y301/11002

Abstract: The invention relates to a method for modifying genetic material in algal cells that includes the use of rare-cutting endonuclease to target specific genomic sequences. In particular, the invention relates to a method for modifying genetic material in algal cells wherein rare-cutting endonuclease, especially a homing endonuclease or a TALE-Nuclease, is expressed over several generations to efficiently modify said target genome sequences.

Abstract translation: 本发明涉及用于修饰藻细胞中的遗传物质的方法，其包括使用稀有内切核酸酶来靶向特定的基因组序列。 具体而言，本发明涉及用于修饰藻细胞中的遗传物质的方法，其中稀有切割内切核酸酶，特别是归巢内切核酸酶或TALE-核酸酶在数代中表达以有效修饰所述靶基因组序列。

公开(公告)号：WO2012106353A2

公开(公告)日：2012-08-09

申请号：PCT/US2012023351

申请日：2012-01-31

Applicant: UNIV OKLAHOMA ,  DEANGELIS PAUL L ,  OTTO NIGEL J

Inventor： DEANGELIS PAUL L ,  OTTO NIGEL J

IPC: C08B37/00 ,  C08B37/08 ,  C08B37/10 ,  C12N15/52 ,  C12N15/63 ,  C12P19/04

CPC classification number: C12P19/26 ,  C08B37/0063 ,  C12N9/0006 ,  C12N9/1051 ,  C12N9/1241 ,  C12N15/52 ,  C12P19/04 ,  C12Y101/01022 ,  C12Y207/07009

Abstract: Testosteronan, a heparosan analog having the structure [-4-D-GlcUA-a1,4-D-GlcNAc-a1-]n, is produced by testosteronan synthase, a single protein that is a dual-action catalyst that utilizes UDP-GlcUA and UDP-GlcNAc to synthesize a polysaccharide having the structure [-4-D-GlcUA- a1,4-D-GlcNAc-a1-]n.

Abstract translation: 具有[-4-D-GlcUA-a1,4-D-GlcNAc-a1-] n结构的heparosan类似物的睾酮是由睾酮合成酶产生的，睾酮合成酶是使用UDP-GlcUA的双重作用催化剂的单一蛋白质 和UDP-GlcNAc合成具有[-4-D-GlcUA-a1,4-D-GlcNAc-a1-] n结构的多糖。

公开(公告)号：WO2012032154A1

公开(公告)日：2012-03-15

申请号：PCT/EP2011/065642

申请日：2011-09-09

Applicant: FIDIA FARMACEUTICI S.P.A. ,  CORSA, Vincenza ,  NEGRO, Alessandro ,  VACCARO, Susanna ,  MESSINA, Luciano

Inventor： CORSA, Vincenza ,  NEGRO, Alessandro ,  VACCARO, Susanna ,  MESSINA, Luciano

IPC: C08B37/00 ,  C12N9/10 ,  C12P19/04 ,  C12P19/26 ,  C12N15/63 ,  C12N15/75

CPC classification number: C12P19/26 ,  C08B37/0072 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/1051 ,  C12N9/1241 ,  C12N9/92 ,  C12N15/635 ,  C12N15/75 ,  C12Y101/01022 ,  C12Y204/01212 ,  C12Y207/07009 ,  C12Y503/01009

Abstract: The present invention relates to a method for the production of hyaluronic acid (HA) in Bacillus subtilis and Escherichia coli through plasmid vectors wherein the gene is under the control of strong promoter P grac , and a system for the selection of stable bacterial strains for the production of high levels of hyaluronic acid.

Abstract translation: 本发明涉及通过质粒载体在枯草芽孢杆菌和大肠杆菌中生产透明质酸（HA）的方法，其中该基因处于强启动子Pgrac的控制下，以及用于选择用于生产的稳定细菌菌株的系统 的高水平透明质酸。

公开(公告)号：WO2012032153A1

公开(公告)日：2012-03-15

申请号：PCT/EP2011/065641

申请日：2011-09-09

Applicant: FIDIA FARMACEUTICI S.P.A. ,  CORSA, Vincenza ,  NEGRO, Alessandro ,  BISECCHIA, Sonia

Inventor： CORSA, Vincenza ,  NEGRO, Alessandro ,  BISECCHIA, Sonia

IPC: C08B37/00 ,  C12N9/10 ,  C12P19/04 ,  C12P19/26 ,  C12N15/63 ,  C12N15/75

CPC classification number: C12P19/04 ,  C08B37/0072 ,  C12N9/0006 ,  C12N9/1051 ,  C12N9/1241 ,  C12N9/92 ,  C12N15/75 ,  C12P19/26 ,  C12Y101/01022 ,  C12Y204/01212 ,  C12Y207/07009 ,  C12Y503/01009

Abstract: The present invention relates to a method of producing hyaluronic acid (HA) in Escherichia coli and Bacillus megaterium through episomal plasmid vectors wherein the gene is under the control of strong promoter T7, preferably under the control of strong promoter T7 of bacteriophage T7, and a system for the selection of stable bacterial strains producing high levels of hyaluronic acid.

Abstract translation: 本发明涉及通过附加型质粒载体在大肠杆菌和巨大芽孢杆菌中生产透明质酸（HA）的方法，其中该基因处于强启动子T7的控制下，优选在噬菌体T7的强启动子T7的控制下，和 用于选择产生高水平透明质酸的稳定细菌菌株的系统。

公开(公告)号：WO1998022614A1

公开(公告)日：1998-05-28

申请号：PCT/JP1997004159

申请日：1997-11-14

Applicant: YAMASA CORPORATION ,  NOGUCHI, Toshitada ,  SHIBA, Toshikazu

Inventor： YAMASA CORPORATION

IPC: C12P19/30

CPC classification number: C12N9/1229 ,  C12N9/1241 ,  C12N9/90 ,  C12P19/30 ,  C12Y207/04001 ,  C12Y207/07009 ,  C12Y501/03002 ,  Y02P20/582

Abstract: A process for producing nucleoside 5'-triphosphates (NTP) from nucleoside 5'-diphosphates (NDP) other than adenosine 5'-diphosphate, characterized by using a polyphosphate kinase as an enzyme and polyphosphoric acid as the phosphate donor; and application of this process to various glycosylation reactions. This process makes it possible to conveniently and economically synthesize NTP from NDP enzymatically. Also, it becomes possible thereby to economically recycle and synthesize sugar nucleotides and synthesize, for example, oligosaccharides associating therewith without resort to expensive phosphoenolpyruvic acid, ATP, etc. in the reactions for reproducing or converting NDP into NTP in the systems for enzymatically synthesizing oligosaccharides by combining with the synthesis of oligonucleotides, etc.

Abstract translation: 以5'-二磷酸腺苷以外的核苷5'-二磷酸（NDP）生产核苷5'-三磷酸（NTP）的方法，其特征在于使用多磷酸激酶作为酶，多磷酸作为磷酸盐供体; 并将该方法应用于各种糖基化反应。 该方法使得可以方便和经济地从NDP酶合成NTP。 此外，通过在用于酶促合成寡糖的体系中，在NDP再生或将NDP转化为NTP的反应中合成糖核苷酸并合成例如与其缔合的寡糖而不需要昂贵的磷酸烯醇丙酮酸，ATP等， 通过与寡核苷酸的合成等组合