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    METHOD FOR TARGETED MODIFICATION OF ALGAE GENOMES
    2.
    发明申请
    METHOD FOR TARGETED MODIFICATION OF ALGAE GENOMES 审中-公开
    用于藻类基因组定向修饰的方法

    公开(公告)号：WO2014076571A2

    公开(公告)日：2014-05-22

    申请号：PCT/IB2013002974

    申请日：2013-11-18

    Applicant: CELLECTIS ,  TOTAL MARKETING SRRVICES

    Inventor： DUCHATEAU PHILIPPE ,  DABOUSSI FAYZA

    IPC: C12N15/82

    CPC classification number: C12N15/8213 ,  C07K14/195 ,  C07K2319/80 ,  C12N9/1241 ,  C12N9/22 ,  C12N15/52 ,  C12R1/89 ,  C12Y207/07009 ,  C12Y301/00 ,  C12Y301/11002

    Abstract: The invention relates to a method for modifying genetic material in algal cells that includes the use of rare-cutting endonuclease to target specific genomic sequences. In particular, the invention relates to a method for modifying genetic material in algal cells wherein rare-cutting endonuclease, especially a homing endonuclease or a TALE-Nuclease, is expressed over several generations to efficiently modify said target genome sequences.

    Abstract translation: 本发明涉及用于修饰藻细胞中的遗传物质的方法，其包括使用稀有内切核酸酶来靶向特定的基因组序列。 具体而言，本发明涉及用于修饰藻细胞中的遗传物质的方法，其中稀有切割内切核酸酶，特别是归巢内切核酸酶或TALE-核酸酶在数代中表达以有效修饰所述靶基因组序列。

    NON-STOCHASTIC GENERATION OF GENETIC VACCINES AND ENZYMES
    3.
    发明申请
    NON-STOCHASTIC GENERATION OF GENETIC VACCINES AND ENZYMES 审中-公开
    遗传疫苗和酶的非生物发生

    公开(公告)号：WO0046344A3

    公开(公告)日：2000-12-28

    申请号：PCT/US0003086

    申请日：2000-02-04

    Applicant: DIVERSA CORP

    Inventor： SHORT JAY M

    IPC: C12N15/09 ,  A61K38/00 ,  A61K39/00 ,  A61K39/39 ,  A61K48/00 ,  A61P33/06 ,  C07K14/445 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/02 ,  C12N5/10 ,  C12N9/00 ,  C12N9/16 ,  C12N9/24 ,  C12N9/38 ,  C12N15/01 ,  C12N15/10 ,  C12P21/02 ,  C12Q1/02 ,  C12Q1/68 ,  G01N33/15 ,  G01N33/50 ,  G01N33/53 ,  G01N33/566 ,  C07H21/02

    CPC classification number: C12N15/1058 ,  A61K39/00 ,  A61K2039/53 ,  C07K14/445 ,  C12N9/00 ,  C12N9/14 ,  C12N9/16 ,  C12N9/88 ,  C12N15/102 ,  C12N15/1027 ,  C12N15/1034 ,  C12Y301/11002

    Abstract: This invention provides methods of obtaining novel polynucleotides and encoded polypeptides by use of non-stochastic methods of directed evolution (DirectEvolution ). These methods include non-stochastic polynucleotide site-saturation mutagenesis (Gene Site Saturation Mutagenesis ) and non-stochastic polynucleotide reassembly (GeneReassembly ). Through use of the claimed methods, genetic vaccines, enzymes, and other desirable molecules can be evolved towards desirable properties. For example, vaccine vectors can be obtained that exhibit increased efficacy for use as genetic vaccines. Vectors obtained by using the methods can have, for example, enhanced antigen expression, increased uptake into a cell, increased stability in a cell, ability to tailor an immune response, and the like. This invention provides methods of obtaining novel enzymes that have optimized physical and/or biological properties. Furthermore, this invention provides methods of obtaining a variety of novel biologically active molecules, in the fields of antibiotics, pharmacotherapeutics, and transgenic traits.

    Abstract translation: 本发明提供了通过使用定向进化的非随机方法（DirectEvolution TM）获得新的多核苷酸和编码的多肽的方法。 这些方法包括非随机多核苷酸位点 - 饱和诱变（Gene Site Saturation Mutagenesis TM）和非随机多核苷酸重组（GeneReassembly TM TM）。 通过使用所要求保护的方法，可以将遗传疫苗，酶和其它所需的分子演变为期望的性质。 例如，可以获得表现出增加用作基因疫苗的功效的疫苗载体。 通过使用该方法获得的载体可具有例如增强的抗原表达，增加的细胞摄取，增加细胞的稳定性，定制免疫应答的能力等。 本发明提供获得具有优化的物理和/或生物学特性的新型酶的方法。 此外，本发明提供了在抗生素，药物治疗剂和转基因性状领域中获得各种新型生物活性分子的方法。

    NON-STOCHASTIC GENERATION OF GENETIC VACCINES AND ENZYMES
    5.
    发明申请
    NON-STOCHASTIC GENERATION OF GENETIC VACCINES AND ENZYMES 审中-公开
    遗传疫苗和酶的非随机发育

    公开(公告)号：WO00046344A2

    公开(公告)日：2000-08-10

    申请号：PCT/US2000/003086

    申请日：2000-02-04

    IPC: C12N15/09 ,  A61K38/00 ,  A61K39/00 ,  A61K39/39 ,  A61K48/00 ,  A61P33/06 ,  C07K14/445 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/02 ,  C12N5/10 ,  C12N9/00 ,  C12N9/16 ,  C12N9/24 ,  C12N9/38 ,  C12N15/01 ,  C12N15/10 ,  C12P21/02 ,  C12Q1/02 ,  C12Q1/68 ,  G01N33/15 ,  G01N33/50 ,  G01N33/53 ,  G01N33/566 ,  C12N

    CPC classification number: C12N15/1058 ,  A61K39/00 ,  A61K2039/53 ,  C07K14/445 ,  C12N9/00 ,  C12N9/14 ,  C12N9/16 ,  C12N9/88 ,  C12N15/102 ,  C12N15/1027 ,  C12N15/1034 ,  C12Y301/11002 ,  Y02A50/412 ,  Y02A50/58

    Abstract: This invention provides methods of obtaining novel polynucleotides and encoded polypeptides by use of non-stochastic methods of directed evolution (DirectEvolution ). These methods include non-stochastic polynucleotide site-saturation mutagenesis (Gene Site Saturation Mutagenesis ) and non-stochastic polynucleotide reassembly (GeneReassembly ). Through use of the claimed methods, genetic vaccines, enzymes, and other desirable molecules can be evolved towards desirable properties. For example, vaccine vectors can be obtained that exhibit increased efficacy for use as genetic vaccines. Vectors obtained by using the methods can have, for example, enhanced antigen expression, increased uptake into a cell, increased stability in a cell, ability to tailor an immune response, and the like. This invention provides methods of obtaining novel enzymes that have optimized physical and/or biological properties. Furthermore, this invention provides methods of obtaining a variety of novel biologically active molecules, in the fields of antibiotics, pharmacotherapeutics, and transgenic traits.

    Abstract translation: 本发明提供了用于制备由非随机定向进化方法（DirectEvolution 3）编码的新型多核苷酸和多肽的方法。 这些方法涉及非随机多核苷酸位点饱和诱变（基因位点饱和诱变3）和非随机多核苷酸重新组装（GeneReassembly 3）。 利用这些要求保护的方法，可以将基因疫苗，酶和其他所需分子升级到所需性质。 例如，可以获得改良的疫苗载体并用作基因疫苗。 用这些方法获得的载体可以表现出例如抗原表达增加，细胞摄取增加，细胞稳定性增加，适应免疫应答的能力等。 此外，本发明涉及用于制备具有优化的物理和/或生物性质的新型酶的方法。 此外，本发明涉及在抗生素，药物治疗剂和转基因性状领域中制备新的生物活性分子的方法。

    METHOD FOR TARGETED MODIFICATION OF ALGAE GENOMES
    6.
    发明申请
    METHOD FOR TARGETED MODIFICATION OF ALGAE GENOMES 审中-公开
    ALGAE基因的目标修饰方法

    公开(公告)号：WO2014076571A3

    公开(公告)日：2014-11-06

    申请号：PCT/IB2013002974

    申请日：2013-11-18

    Applicant: CELLECTIS ,  TOTAL MARKETING SERVICES

    Inventor： DUCHATEAU PHILIPPE ,  DABOUSSI FAYZA

    IPC: C12N15/82 ,  C12N9/22

    CPC classification number: C12N15/8213 ,  C07K14/195 ,  C07K2319/80 ,  C12N9/1241 ,  C12N9/22 ,  C12N15/52 ,  C12R1/89 ,  C12Y207/07009 ,  C12Y301/00 ,  C12Y301/11002

    Abstract: The invention relates to a method for modifying genetic material in algal cells that includes the use of rare-cutting endonuclease to target specific genomic sequences. In particular, the invention relates to a method for modifying genetic material in algal cells wherein rare-cutting endonuclease, especially a homing endonuclease or a TALE-Nuclease, is expressed over several generations to efficiently modify said target genome sequences.

    Abstract translation: 本发明涉及一种在藻类细胞中修饰遗传物质的方法，包括使用稀有切割内切核酸酶靶向特定基因组序列。 特别地，本发明涉及用于修饰藻类细胞中的遗传物质的方法，其中稀释切割内切核酸酶，特别是归巢内切核酸酶或TALE-核酸酶在几代中表达以有效修饰所述靶基因组序列。

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