公开(公告)号：WO1997045202A1

公开(公告)日：1997-12-04

申请号：PCT/FR1997000912

申请日：1997-05-23

Applicant: BIO MERIEUX ,  ELAISSARI, Abdelhamid ,  PICHOT, Christian ,  MANDRAND, Bernard ,  SAUZEDDE, Florence

Inventor： BIO MERIEUX

IPC: B03C01/01

CPC classification number: H01F1/37 ,  B03C1/01 ,  G01N33/5375 ,  G01N33/5434 ,  G01N2446/10 ,  G01N2446/40 ,  G01N2446/84 ,  G01N2446/86 ,  H01F1/44 ,  Y10S436/806 ,  Y10S436/824 ,  Y10T428/2982 ,  Y10T428/2989 ,  Y10T428/2991 ,  Y10T428/2998 ,  Y10T436/24

Abstract: The invention discloses superparamagnetic monodispersed particles, their method of preparation and their uses. These particles comprise: a core of a first polymer, an internal layer of a second polymer coating the core, and in which is distributed a magnetic material, and an external layer of a third polymer coating the magnetic layer, and capable of interacting with at least one biological molecule, at least the second polymer being heat sensitive and having a predetermined lower critical solubility temperature (LCST) of 15 to 65 DEG C.

Abstract translation: 本发明公开了超顺磁单分散颗粒，其制备方法及其用途。 这些颗粒包括：第一聚合物的芯，涂覆芯的第二聚合物的内层，其中分布有磁性材料，以及涂覆磁性层的第三聚合物的外层，并且能够与 至少一个生物分子，至少第二聚合物是热敏感的并具有15至65℃的预定的较低临界溶解温度（LCST）。

公开(公告)号：WO9602235A1

公开(公告)日：1996-02-01

申请号：PCT/US9508861

申请日：1995-07-14

Applicant: BIOQUEST INC

Inventor： CHAGNON MARK S ,  BURKLE STEPHEN E ,  CARTER MICHELLE J ,  HAMILTON TRACY J ,  HAVELICK JOHN JR ,  KAPLAN DEBORAH A ,  MARZLOFF KRISTIN L

IPC: A61K9/127 ,  A61K47/48 ,  A61K49/18 ,  B03C1/01 ,  C12N11/14 ,  G01N33/543 ,  G01N33/551 ,  G01N33/554 ,  A61K9/16 ,  A61K9/50

CPC classification number: G01N33/554 ,  A61K9/1272 ,  A61K47/6923 ,  A61K49/1812 ,  A61K49/183 ,  A61K49/1854 ,  A61K49/186 ,  B03C1/01 ,  C12N11/14 ,  G01N33/54313 ,  G01N33/5434 ,  G01N33/551 ,  G01N2446/20 ,  G01N2446/80 ,  G01N2446/84 ,  G01N2446/90

Abstract: Novel N-RES agents for use in vitro and in vivo medical and biological applications, such as, for example MRI and/or intravenous administration of pharmaceutical agents are described. The novel N-RES agents are prepared by coating inorganic core particles with a poly(acrylic acid) - poly(alkylene ether) graft copolymer. The resulting polymer-coated inorganic particle cores are useful as imaging agents, therapeutic agents or carriers having increased circulation time due to decrease uptake or recognition by the RES.

Abstract translation: 描述了用于体外和体内医学和生物应用的新型N-RES试剂，例如药物的MRI和/或静脉内施用。 通过用聚（丙烯酸） - 聚（亚烷基醚）接枝共聚物涂覆无机芯颗粒来制备新的N-RES试剂。 得到的聚合物涂覆的无机颗粒芯可用作成像剂，治疗剂或载体，由于减少摄取或RES的识别而具有增加的循环时间。

公开(公告)号：WO1991009678A1

公开(公告)日：1991-07-11

申请号：PCT/US1990007492

申请日：1990-12-18

Applicant: OMNI QUEST CORPORATION

Inventor： OMNI QUEST CORPORATION ,  CHAGNON, Mark, S.

IPC: B03C01/00

CPC classification number: B82Y5/00 ,  A61K49/183 ,  A61K49/1833 ,  B03C1/01 ,  G01N33/54313 ,  G01N33/5434 ,  G01N33/551 ,  G01N2446/20 ,  G01N2446/80 ,  G01N2446/84 ,  G01N2446/90

Abstract: Magnetically responsive particles and to their use in systems in which the separation of certain molecules from the surrounding medium is necessary or desirable are disclosed. The magnetically responsive particles consist of a metal, metal oxide or metal alloy core, coated with an organo-metallic polymer having attached thereto an organic functionality to which a variety of organic and/or biological molecules can be coupled. The particles can be dispersed in aqueous media without rapid gravitational settling and conveniently reclaimed from the media using a magnetic field. The magnetically responsive particles of the invention may be coupled to biological or organic molecules with affinity for, or the ability to absorb, or which interact with certain other biological or organic molecules. Particles so coupled may be used in a variety of in vitro or in vivo systems involving separations steps or the directed movement of coupled molecules to particular sites, including immunological assays, other biological assays, biochemical or enzymatic reactions, affinity chromatographic purification, cell sorting and diagnostic and therapeutic uses.

Abstract translation: 公开了磁响应颗粒及其在某些分子与周围介质的分离是必需或期望的系统中的用途。 磁响应颗粒由金属，金属氧化物或金属合金芯组成，涂覆有有机金属聚合物，其附着有有机官能团，各种有机和/或生物分子可以偶合到其上。 颗粒可以分散在水介质中，而不需要快速重力沉降，并且可以方便地使用磁场从介质中回收。 本发明的磁响应性颗粒可以与生物或有机分子偶联，具有与某些其它生物或有机分子的亲和力或吸收能力或与之相互作用的生物或有机分子。 如此偶联的颗粒可用于各种体外或体内系统，包括分离步骤或偶联分子到特定位点的定向运动，包括免疫测定，其他生物测定，生物化学或酶反应，亲和层析纯化，细胞分选和 诊断和治疗用途。

公开(公告)号：WO2004042397A1

公开(公告)日：2004-05-21

申请号：PCT/JP2003/005733

申请日：2003-05-08

Applicant: 科学技術振興事業団 ,  吉永 耕二 ,  円福 敬二

Inventor： 吉永 耕二 ,  円福 敬二

IPC: G01N33/541

CPC classification number: G01N33/5434 ,  G01N2446/84 ,  G01N2446/86

Abstract: SQUID磁気センサーによる免疫反応の測定に用いられるのに好適な高感度の磁性マーカーとその作製に関する新しい技術を開示している。磁性微粒子とその周りを被覆するポリマーとから構成され、SQUID磁気センサーにより免疫反応を測定するのに用いられる磁性マーカーにおいて、磁性微粒子(好ましくはフェライトFe 3 O 4 )の粒子径が20～40nmであり、磁性マーカーの外径が40～100nmであり、さらに、ポリマーの表面にカルボキシル基を有するようにする。このSQUID磁気センサー用磁性マーカーは、磁性微粒子の表面に、末端に重合性ビニル基を有し親水性で分子量が500～1000のマクロモノマー(好ましくは、ポリビニルピロリドン)を吸着させた後、カルボキシル基を有し親水性のビニル化合物から成るモノマーと架橋剤とを加えて共重合させることによって作製することができる。

Abstract translation: 公开了一种优选用于通过使用SQUID磁传感器测量免疫应答的高灵敏度磁性标记和关于生产的新技术。 由磁性粒子和聚合物组成的磁性标记物，其表面用SQUID磁性传感器测量免疫应答。 磁性粒子（优选铁氧体Fe 3 O 4）的粒径为20〜40nm。 磁标记的外径为40〜100nm。 聚合物的表面含有羧基。 磁性标记是通过使磁性颗粒的表面吸收具有可聚合乙烯基并具有500至1,000的分子量的亲水性大分子单体（优选聚乙烯吡咯烷酮），加入具有羧基的单体并包含亲水性乙烯基 化合物和交联剂，并使它们共聚。

公开(公告)号：WO1996040502A1

公开(公告)日：1996-12-19

申请号：PCT/US1996005058

申请日：1996-04-12

Applicant: IMMUNIVEST CORPORATION

Inventor： IMMUNIVEST CORPORATION ,  LIBERTI, Paul, A. ,  RAO, Galla, C. ,  CHIARAPPA, Joseph, N. ,  PICCOLI, Steven, P.

IPC: B32B05/16

CPC classification number: B03C1/01 ,  A61K9/5094 ,  G01N33/5434 ,  G01N33/54393 ,  G01N2333/765 ,  G01N2446/20 ,  G01N2446/80 ,  G01N2446/84 ,  G11B5/842 ,  G11B5/848 ,  H01F1/445 ,  Y10T428/2982

Abstract: The invention provides methodology for the direct coating of transiently stable, colloidal particulate magnetic substrates with various synthetic or naturally occurring polymers, particularly biologically active peptides and proteins.

Abstract translation: 本发明提供了用各种合成或天然存在的聚合物，特别是生物活性肽和蛋白质直接涂覆瞬时稳定的胶体颗粒状磁性底物的方法。

公开(公告)号：WO1989004373A1

公开(公告)日：1989-05-18

申请号：PCT/US1988003666

申请日：1988-10-18

Applicant: BAXTER INTERNATIONAL INC.

Inventor： BAXTER INTERNATIONAL INC. ,  WANG, Chao-Huei, J. ,  SHAH, Dinesh, O.

IPC: C12Q01/68

CPC classification number: B01J20/28016 ,  A61M1/3618 ,  B01J20/28004 ,  B01J20/28009 ,  C08F257/02 ,  C08F285/00 ,  C12Q1/6834 ,  G01N33/54326 ,  G01N33/5434 ,  G01N33/582 ,  G01N33/585 ,  G01N2446/10 ,  G01N2446/40 ,  G01N2446/84 ,  G01N2446/86 ,  C12Q2563/143 ,  C12Q2563/107

Abstract: This invention provides a novel process of producing magnetically responsive polymer particles comprising polymeric core particles coated evenly with a layer of polymer containing magnetically responsive metal oxide. A wide variety of polymeric particles with sizes ranging from 1 to 100 microns can be used as core particles and transformed into magnetically responsive polymer particles. The surface of these magnetically responsive polymer particles can be coated further with another layer of functionalized polymer. These magnetically responsive polymer particles can be used for passive or covalent coupling of biological material such as antigens, antibodies, enzymes or DNA/RNA hybridization and used as solid phase for various types of immunoassays, DNA/RNA hybridization probes assays, affinity purification, cell separation and other medical, diagnostic, and industrial applications.

公开(公告)号：WO2012156059A1

公开(公告)日：2012-11-22

申请号：PCT/EP2012/002037

申请日：2012-05-11

Applicant: UNIVERSITÄT BAYREUTH ,  MÜLLER, Axel ,  FREITAG, Ruth ,  SYNATSCHKE, Christopher Volker ,  MAJEWSKI, Alexander ,  JÉROME, Valèrie ,  SCHALLON, Anja

Inventor： MÜLLER, Axel ,  FREITAG, Ruth ,  SYNATSCHKE, Christopher Volker ,  MAJEWSKI, Alexander ,  JÉROME, Valèrie ,  SCHALLON, Anja

IPC: G01N33/543 ,  A61K48/00 ,  C12N15/87

CPC classification number: G01N33/54333 ,  G01N2446/84

Abstract: The present invention relates to a method for obtaining intracellular molecules having specific affinity for a polyelectrolyte particle comprising a functionalized magnetic nanoparticle by transferring said polyelectrolyte particle into a eukaryotic cell comprising the intracellular molecule, wherein a conjugate is formed between the polyelectrolyte particle and the intracellular molecule. By recovering the conjugate from the eukaryotic cell and isolating the intracellular molecule from the conjugate, the intracellular molecule can be obtained and subsequently analyzed. The present invention further relates to the use of such a polyelectrolyte particle for identifying and/or isolating a subset of eukaryotic cells comprising the polyelectrolyte particle from a plurality of eukaryotic cells, and for isolating a complex formed between the polyelectrolyte particle and a polyanion from polyelectrolyte particles not in complex with the polyanion. Furthermore, the present invention relates to the use of an intracellular molecule for modifying the expression of a nucleic acid transfected into a eukaryotic cell by means of such a polyelectrolyte particle.

Abstract translation: 本发明涉及通过将所述聚电解质颗粒转移到包含细胞内分子的真核细胞中获得对包含官能化磁性纳米颗粒的聚电解质颗粒具有特异性亲和力的细胞内分子的方法，其中在聚电解质颗粒和细胞内分子之间形成缀合物 。 通过从真核细胞中回收缀合物并从缀合物中分离细胞内分子，可以获得细胞内分子并随后进行分析。 本发明还涉及这种聚电解质颗粒用于鉴定和/或分离来自多个真核细胞的包含聚电解质颗粒的真核细胞子集，并用于从聚电解质中分离聚电解质颗粒和聚阴离子之间形成的络合物 颗粒与聚阴离子不复合。 此外，本发明涉及细胞内分子用于通过这种聚电解质颗粒修饰转染到真核细胞中的核酸的表达的用途。

公开(公告)号：WO2003033159A1

公开(公告)日：2003-04-24

申请号：PCT/JP2002/010590

申请日：2002-10-11

Applicant: 学校法人日本大学 ,  神野 英毅

Inventor： 神野 英毅

IPC: B03C1/00

CPC classification number: G01N33/545 ,  B03C1/01 ,  C12N11/14 ,  G01N33/54326 ,  G01N2446/84 ,  H01F1/112 ,  H01F1/14758 ,  H01F1/36 ,  Y10S428/90 ,  Y10T428/2991 ,  Y10T428/2998

Abstract: Magnetic particles which comprise a particulate magnetic material and, formed on the surface thereof, an readily decomposable or readily soluble polymer coating layer containing a reactive component; and a method for producing a reaction product using the magnetic particles. The use of the above magnetic particles allows the combination of the efficient progress of a solid state reaction and the easiness of the recovery of reaction product.

Abstract translation: 包含微粒磁性材料并在其表面上形成容易分解或容易溶解的含有反应性组分的聚合物涂层的磁性颗粒; 以及使用该磁性粒子的反应产物的制造方法。 使用上述磁性颗粒可以使固态反应的有效进展与反应产物的回收的容易性相结合。

公开(公告)号：WO99062079A1

公开(公告)日：1999-12-02

申请号：PCT/IL1999/000275

申请日：1999-05-24

IPC: A61K9/51 ,  A61K47/00 ,  A61K49/00 ,  A61K49/18 ,  B01D43/00 ,  B03C1/01 ,  G01N33/543 ,  H01F1/00 ,  H01F1/33 ,  H01F1/44

CPC classification number: B82Y25/00 ,  A61K49/1881 ,  B03C1/01 ,  B82Y5/00 ,  G01N33/5434 ,  G01N2446/20 ,  G01N2446/30 ,  G01N2446/84 ,  H01F1/0054 ,  H01F1/445

Abstract: A method for preparing nanoparticles coated with magnetic metal oxide, comprising the following steps: a) contacting an aqueous solution containing a soluble polymeric metal chelating agent with one or more soluble metal salts providing metal ions, wherein at least one of said metal ions is capable of forming an oxide which is magnetic, said metal ions being in amounts which do not exceeed substantially the binding capacity of said chelating agent; b) causing said metal ions to be present in the oxidation states required for the formation of the oxide which is magnetic; c) maintaining the pH of the solution at the range of at least 7; d) introducing into the solution additional amounts of said metal salts; e) causing said additional metal ions to be present in the oxidation states required for the formation of the oxide which is magnetic; f) maintaining the pH of the solution at the range of at least 7; g) successively repeating the operations of steps d) to f) as many times as required to obtain monodispersed nanoparticles coated with magnetic metal oxide.

Abstract translation: 一种制备用磁性金属氧化物涂覆的纳米颗粒的方法，包括以下步骤：a）使含有可溶性聚合金属螯合剂的水溶液与提供金属离子的一种或多种可溶性金属盐接触，其中所述金属离子中的至少一种能够 形成磁性的氧化物，所述金属离子的量基本上不超过所述螯合剂的结合能力; b）使所述金属离子以形成磁性氧化物所需的氧化态存在; c）将溶液的pH保持在至少7的范围内; d）向溶液中引入额外量的所述金属盐; e）使所述另外的金属离子以形成磁性的氧化物所需的氧化态存在; f）将溶液的pH保持在至少7的范围; g）依次重复步骤d）至f）的操作，以获得涂覆有磁性金属氧化物的单分散纳米颗粒所需的次数。

公开(公告)号：WO9421240A3

公开(公告)日：1994-10-13

申请号：PCT/DE9400314

申请日：1994-03-17

Applicant: SILICA GEL GMBH ,  PILGRIMM HERBERT

Inventor： PILGRIMM HERBERT

IPC: A61N1/40 ,  A61K9/50 ,  A61K31/395 ,  A61K31/66 ,  A61K31/77 ,  A61K35/12 ,  A61K35/74 ,  A61K35/76 ,  A61K38/43 ,  A61K39/395 ,  A61K41/00 ,  A61K47/02 ,  A61K49/00 ,  A61K49/18 ,  A61N2/00 ,  A61P35/00 ,  A61P37/00 ,  B03C1/01 ,  C07F9/09 ,  C07H21/00 ,  C12N11/14 ,  C12Q1/68 ,  G01N33/543 ,  G01R33/28 ,  H01F1/00 ,  H01F1/36 ,  H01F1/44

CPC classification number: C07F9/091 ,  A61K9/5094 ,  A61K41/0052 ,  A61K49/186 ,  A61K49/1887 ,  B03C1/01 ,  B82Y5/00 ,  B82Y25/00 ,  B82Y30/00 ,  C07H21/00 ,  C12N11/14 ,  C12Q1/6834 ,  G01N33/5434 ,  G01N2446/20 ,  G01N2446/84 ,  G01N2446/86 ,  G01R33/281 ,  H01F1/0054 ,  H01F1/36 ,  H01F1/44 ,  H01F1/445

Abstract: New superparamagnetic particles useful in medicine for destroying tumors, increasing immunity and diagnosing conditions are disclosed. For that purpose, very small superparamagnetic single-domain particles are aggregated and protected against further aggregation by chemical bonding of reactive stabilizer substances on the surface of the superparamagnetic particles. These new particles thus consist of stable, decomposable aggregates with a particle size in a range between 10 and 1000 nanometres and a defined behaviour in a magnetic field. The aggregates consist of several small superparamagnetic single-domain particles of iron oxide, iron mixed oxide or iron, with a particle size in a range between 3 and 20 nanometres, bearing on their surface chemically bound organic substances from the group comprising the phosphate, diphosphate, polyphosphate, thiophosphate, phosphonate or group containing polyalkylene glycols, phosphate group containing nucleotides, their oligomers or polymers, as well as phosphate group containing carbohydrates, which may present further binding sites. Both the new disclosed superparamagnetic aggregates and reactive stabilizer substances may be active substances.