The invention relates to a road-rail co-layer mixed-beam suspension cable-stayed cooperation bridge. The bridge comprises a main beam and at least two bridge towers, wherein the two bridge towers aresequentially arranged along the bridge direction, a road bridge deck and an rail bridge deck are arranged on the main beam in the same layer, the two adjacent bridge towers are connected through a main cable, and the main cable is connected with main beam bodies below through a sling; and each bridge tower is connected with main beam bodies on the two sides through an stay cable. According to theroad-rail co-layer mixed-beam suspension cable-stayed cooperation bridge, a suspension structure of the main cable plus the sling and a cable-stay structure of the stay cable are combined, the advantages of a suspension bridge and a cable-stayed bridge are brought into full play, thus effective improving the rigidity of 1/4 of the span and span area structure; in addition, the main beam is large in overall rigidity and reasonable in stress, so that the span of a bridge can be improved to 1600 m, the overall rigidity of the structure is large, and accordingly requirements of railway track shapedeformation can be fully met.