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45 条记录 (耗时 0.017 秒)

    ESTERASE MUTANT AND USE THEREOF
    11.
    发明公开
    ESTERASE MUTANT AND USE THEREOF 审中-实审

    公开(公告)号：EP4365286A1

    公开(公告)日：2024-05-08

    申请号：EP21951543.4

    申请日：2021-09-18

    申请人： Asymchem Laboratories (Tianjin) Co., Ltd.

    发明人： HONG, Hao ,  JAMES, Gage ,  XIAO, Yi ,  ZHANG, Na ,  JIAO, Xuecheng ,  YANG, Yiming ,  LI, Juan ,  HUANG, Qixing

    IPC分类号： C12N9/16 ,  C12N15/55 ,  C12N15/70 ,  C12N1/21 ,  C12P7/40 ,  C12P7/02 ,  C12R1/19

    摘要： Provided are an esterase mutant and the use thereof. The esterase mutant obtained by means of rational design and several rounds of evolution screening with enzymes on the basis of an amino acid sequence as shown in SEQ ID NO: 1 is changed in terms of protein structure and function compared with a wild-type esterase; in practical use, the catalytic activity and/or stereoselectivity of the esterase mutant is greatly improved; and when a system contains some organic cosolvents, the esterase mutant still has relatively stable catalytic activity and/or stereoselectivity. In addition, the improvement of the catalytic activity and/or stereoselectivity of the esterase mutant reduces the use amount of the enzyme to a certain extent and reduces the difficulty of post-treatment, and therefore the esterase mutant is suitable for industrial production.

    METHOD FOR SYNTHESIZING CHIRAL DIAMINE COMPOUND
    13.
    发明公开
    METHOD FOR SYNTHESIZING CHIRAL DIAMINE COMPOUND 审中-公开

    公开(公告)号：EP4112733A1

    公开(公告)日：2023-01-04

    申请号：EP20921866.8

    申请日：2020-03-31

    申请人： Asymchem Laboratories (Tianjin) Co., Ltd.

    发明人： HONG, Hao ,  JAMES, Gage ,  XIAO, Yi ,  ZHANG, Na ,  LI, Xiang ,  JIANG, Xiangjun ,  ZHAO, Tong

    IPC分类号： C12P13/00 ,  C12P17/12 ,  C12P17/04 ,  C12N9/10

    摘要： Provided is a method for synthesizing a chiral diamine compound. The synthesizing method includes: converting a substrate represented by Formula I into a chiral diamine compound in Formula I by using a transaminase, herein n=1~10, an R group represents an alkyl, a cycloalkyl, a heteroatom-containing alkyl, a heteroatom-containing cycloalkyl, a heteroatom-containing aryl, an amide compound residue, or an ether compound residue, and a hetero atom is at least one from among O, S and N; R1 and R2 are the same or not the same, the R1 and R2 are respectively and independently hydrogen, a C1-C3 alkyl, or an amino protecting group; and the transaminases are derived from a plurality of strains.The transaminases all have a relatively high reaction selectivity and activity on various substrates represented by Formula 1. By catalyzing and synthesizing a chiral diamine compound by using the biological enzyme, not only is the substrate scope wider, but the route also short and product yield high, thus greatly reducing production costs and reducing the production of an organic solvent and the three wastes.

    METHOD AND APPARATUS FOR CONTINUOUS SYNTHESIS OF CYCLOPROPANE COMPOUNDS
    14.
    发明公开
    METHOD AND APPARATUS FOR CONTINUOUS SYNTHESIS OF CYCLOPROPANE COMPOUNDS 审中-公开

    公开(公告)号：EP3995477A1

    公开(公告)日：2022-05-11

    申请号：EP19936457.1

    申请日：2019-07-02

    申请人： Asymchem Laboratories (Tianjin) Co., Ltd.

    发明人： HONG, Hao ,  LU, Jiangping ,  FENG, Xichun ,  SUN, Xingfang ,  ZHANG, Feng

    IPC分类号： C07C2/86 ,  C07C13/04 ,  C07C245/16 ,  C07B37/02

    摘要： The present disclosure discloses a method and a device for continuously synthesizing cyclopropane compounds. The method includes the following steps: continuously performing a synthetic reaction of a diazomethane precursor in a first reactor, the reaction product of the first reactor flowing into a separator for stratification, the organic phase obtained by stratification overflowing into a second reactor, continuously consuming the diazomethane precursor in a second reactor to prepare diazomethane and performing an electron-rich monoolefin cyclopropanation reaction in situ so as to obtain the cyclopropane compound. The technical solution of the present disclosure may be applied to achieve automatic control, decrease the transfer of high-risk materials, and prevent the risk of pipeline transfer of diazomethane solution, and effectively improve production safety. Moreover, the present disclosure provides a simple device to save equipment investment, and may safely and quantitatively achieve the production of diazomethane and the cyclopropanation reaction of olefins simultaneously.

    PREPARATION METHOD FOR POLYPEPTIDE
    20.
    发明公开
    PREPARATION METHOD FOR POLYPEPTIDE 审中-公开

    公开(公告)号：EP4328316A1

    公开(公告)日：2024-02-28

    申请号：EP21940283.1

    申请日：2021-05-31

    申请人： Asymchem Laboratories (Tianjin) Co., Ltd

    发明人： HONG, Hao ,  JAMES, Gage ,  XIAO, Yi ,  ZHANG, Na ,  JIAO, Xuecheng ,  ZHAO, Junqi ,  WANG, Lei ,  MENG, Xiangyu ,  ZHANG, Mujiao

    IPC分类号： C12P21/02 ,  C12P21/06

    摘要： Provided is a preparation method for a polypeptide. The preparation method includes the following steps: constructing an engineering strain for fusion-expressing a polypeptide gene with a Sumo tag, and inducing the engineering strain for the soluble expression of a polypeptide; obtaining a fusion protein containing a polypeptide precursor from the engineering strain by purification; cleaving the fusion protein containing the polypeptide precursor by a Ulp1 protease to remove the Sumo tag; and purifying a cleavage product of the Ulp1 protease by a method of acetonitrile combined with heating precipitation or a method of precipitation with hexafluoroisopropanol to obtain the polypeptide. By establishing an efficient technology based on soluble recombinant expression of a medicinal polypeptide or a precursor thereof and establishing a simple purification process based on one-step precipitation purification, further combined with HPLC refinement, a polypeptide purity of over 97% can be achieved.