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    METHOD OF DETERMINING DNA BASE SEQUENCE
    11.
    发明公开
    METHOD OF DETERMINING DNA BASE SEQUENCE 审中-公开
    VERFAHREN ZUR BESTIMMUNG EINER DNA-BASENSEQUENZ

    公开(公告)号：EP1413628A1

    公开(公告)日：2004-04-28

    申请号：EP02733397.0

    申请日：2002-06-07

    申请人： Nippon Genetech Co. Ltd.

    发明人： WATAHIKI, Masanori, c/o Toyama Laboratory ,  YONEDA, Yuko, c/o Toyama Laboratory

    IPC分类号： C12Q1/68 ,  C12N15/09

    CPC分类号： C12Q1/6869 ,  C12Q2531/143 ,  C12Q2521/119

    摘要： A transcriptional sequence method whereby a high SN ratio can be obtained in sequence analysis with the use of capillary and longer and more accurate base sequence data can be obtained in a single reaction. More specifically, a method of determining a DNA base sequence involving the step of obtaining a nucleic acid transcription product with the use of an RNA polymerase, a template DNA having a promoter sequence for the RNA polymerase and substrates of the RNA polymerase. The substrates of the RNA polymerase involve a 3'-deoxynucleotide derivative. The RNA polymerase is a mutant RNA polymerase derived from a wild type RNA polymerase by substitution of at least one amino acid or deletion of at least one amino acid. The substitution and/or deletion of the amino acid(s) are performed so that the mutant RNA polymerase has an enhanced capability of incorporating the 3'-deoxynucleotide derivative as a substrate compared with the corresponding wild type RNA polymerase.

    摘要翻译： DNA的转录测序，包括使用具有增强的3'-脱氧核苷酸掺入能力的修饰的RNA聚合酶获得核酸转录产物，包括RNA聚合酶的启动子序列的模板DNA和用于RNA聚合酶的底物是新的。 RNA聚合酶是通过取代或缺失一个或多个氨基酸残基修饰的野生型RNA聚合酶。

    Method for detecting transcribed genomic DNA sequences
    12.
    发明公开
    Method for detecting transcribed genomic DNA sequences 有权
    用于检测转录的基因组DNA序列的方法

    公开(公告)号：EP1174521A3

    公开(公告)日：2002-04-10

    申请号：EP01116674.1

    申请日：2001-07-16

    申请人： TOSOH CORPORATION

    发明人： Ishiguro, Takahiko ,  Yasukawa, Kiyoshi

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6809 ,  C12Q1/68 ,  C12Q2563/173 ,  C12Q2531/143 ,  C12Q2531/113

    摘要： A novel transcriptome analyzing method, provision of a gene found by this method and a protein encoded by said gene. A method for determining whether or not a continued arbitrary DNA sequence existing in the genome of an arbitrary biological species, of which the nucleotide sequence is already known but its possibility of being an expressed region is unclear (specific region), which comprises detecting whether or not a nucleotide sequence that corresponds to the nucleotide sequence of the region is present in the RNA of the biological species, by means of RT-PCR in combination with NASBA and detection using fluorescent labelled oligonucleotide probes.

    USE OF RNA POLYMERASE TO IMPROVE NUCLEIC ACID AMPLIFICATION PROCESS
    13.
    发明授权
    USE OF RNA POLYMERASE TO IMPROVE NUCLEIC ACID AMPLIFICATION PROCESS 失效
    RNA聚合酶，可改善其NUKEINSAEURE酸扩增

    公开(公告)号：EP0776376B1

    公开(公告)日：2001-11-21

    申请号：EP95929679.9

    申请日：1995-07-13

    申请人： Akzo Nobel N.V.

    发明人： SOOKNANAN, Roy

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

    CPC分类号： C12Q1/6865 ,  C12Q2531/143

    摘要： This invention relates to the use of a eukaryotic or prokaryotic DNA- directed RNA polymerase of a class that synthesizes cellular RNA, in a process for the amplification of a specific nucleic acid sequence or of its complement. It also relates to a new process for amplifying a specific nucleic acid sequence. The process includes one or more reactions which may take place in a single reaction vessel. In one instance, in a first reaction, the process includes providing a first RNA polymerase which uses a DNA first template to synthesize an RNA first template, and, in a second reaction, providing the RNA first template and a number of other reagents such that the reagents use the RNA first template to synthesize a DNA second template and an RNA second template. Thereafter a cycle ensues in which the reagents use the RNA second template to synthesize a DNA third template and multiple copies of the RNA second template. The RNA second template is the specific nucleic acid sequence or its complement. This invention includes a kit containing the reagents of this invention.

    Improved method for nucleic acid amplification
    16.
    发明公开
    Improved method for nucleic acid amplification 失效
    改进的用于核酸扩增的方法。

    公开(公告)号：EP0629706A3

    公开(公告)日：1997-12-17

    申请号：EP93202455.7

    申请日：1993-08-20

    申请人： Akzo Nobel N.V.

    发明人： Kievts, Tim ,  Adriaanse, Henriette Maria Aleida ,  Lens, Peter Franklin

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6844 ,  C12Q1/6846 ,  C12Q1/686 ,  C12Q1/6865 ,  C12Q2531/143 ,  C12Q2525/101 ,  C12Q2525/117 ,  C12Q2521/119

    摘要： The present invention relates to an improved method for the amplification of nucleic acid.
    The invention is characterized in that ribonucleotides are introduced during amplification that weaken normal basepairing. Preferably the ribonucleotides are inosine-triphosphate nucleotides which partly substitute guanine-triphosphate nucleotides normally present in the amplification reaction mixture. The incorporation of nucleotides, during amplification, that weaken normal base pairing prevents the formation of secondary structures in the amplificate. The efficiency of the amplification is thereby increased. The introduction, during amplification, of nucleotides that weaken normal base pairing also results in an improved sensitivity during detection of the amplified nucleic acid, when the detection method comprises the hybridization of the amplified nucleic acid to a complementary sequence.

    SOLID PHASE AMPLIFICATION PROCESS.
    17.
    发明公开
    SOLID PHASE AMPLIFICATION PROCESS. 失效
    固相展开方法。

    公开(公告)号：EP0672173A4

    公开(公告)日：1997-06-04

    申请号：EP92922138

    申请日：1992-10-30

    申请人： ADELAIDE CHILDREN S HOSPITAL ,  UNIV SOUTH AUSTRALIA

    发明人： MORRIS CHARLES PHILLIP ,  HARRIS RAYMOND JOHN

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6834 ,  C12Q2565/537 ,  C12Q2531/113 ,  C12Q2531/143 ,  C12Q2531/137

    摘要： An assay and method of use thereof for amplification and detection of target nucleic acids in patient samples, which is rapid, simple and accurate is described. The assay and method utilizes any one of a variety of amplification methods, in combination with primers immobilized onto either the container containing the patient sample, such as a microtiter well plate, or a dipstick which is readily immersible into the container. Amplification occurs in the presence of both the first, immobilized primer and a second, labelled primer which hybridizes to the target nucleic acid in the opposite direction to the first. Examples demonstrate the effectiveness of the method in detecting a specific species of mycoplasma, as well as the gene characteristic of cystic fibrosis.

    Nucleic acid amplification employing transcribable hairpin probe
    19.
    发明公开
    Nucleic acid amplification employing transcribable hairpin probe 失效
    Nukleinsäure-Amplifikation unter Verwendung vontranskriptionsfähigerHaarnadelsonde。

    公开(公告)号：EP0427074A2

    公开(公告)日：1991-05-15

    申请号：EP90120652.4

    申请日：1990-10-27

    申请人： MILES INC.

    发明人： Dattagupta, Nanibhushan

    IPC分类号： C12Q1/68 ,  C12N15/10

    CPC分类号： C12Q1/682 ,  C12Q2531/143 ,  C12Q2525/301 ,  C12Q2561/125

    摘要： Specific nucleic acid sequences are amplified through the use of transcribable hairpin probes. The probe comprises a single stranded self-­complementary sequence which, under hybridizing conditions, forms a hairpin structure having a functional promoter region, and further comprises a transcribable sequence extending from the 5′ end of the hairpin sequence and a probe sequence which may be comprised in the transcribable 5′ sequence or in a sequence extending from the 3′ end of the hairpin sequence. The hairpin form of the probe is transcribable in the presence of a suitable RNA polymerase and appropriate ribonucleoside triphosphates (rNTPs). Amplification is accomplished by hybridizing the desired target nucleic acid sequence with the transcribable probe, transcribing substantially only probe which has become hybridized to the sequence of interest, and allowing transcription to proceed until a desired amount of RNA transcription product has accumu­lated. Optional second stage amplification of the RNA transcripts can produce higher levels of amplification. The amplification method is particularly useful in assays for the detection of particular nucleic acid sequences.

    摘要翻译： 通过使用可转录的发夹探针扩增特异性核酸序列。 探针包含单链自互补序列，其在杂交条件下形成具有功能性启动子区的发夹结构，并且还包含从发夹序列的5分钟末端延伸的可转录序列和可以包含的探针序列 在可转录的5分钟序列中或以从发夹序列的3分钟末端延伸的序列。 探针的发夹形式在合适的RNA聚合酶和适当的核糖核苷三磷酸（rNTP）存在下是可转录的。 通过将期望的靶核酸序列与可转录的探针杂交来实现扩增，基本上仅转录已经与感兴趣的序列杂交的探针，并允许转录进行直到所需量的RNA转录产物积累。 RNA转录物的可选的第二阶段扩增可以产生更高水平的扩增。 扩增方法在用于检测特定核酸序列的测定中特别有用。