-
公开(公告)号：EP3072968A1
公开(公告)日：2016-09-28
申请号：EP16156020.6
申请日：2011-02-22
发明人： POLLACK, Michael G. , ECKHARDT, Allen E. , SUDARSAN, Arjun , SRINIVASAN, Vijay , ROUSE, Jeremy , YI, Uichong , TROTTA, Nicholas , DHOPESHWARKAR, Rahul , THWAR, Prasanna , SMITH, Gregory F. , NORTON, Scott , LINNARTZ, Peter
CPC分类号： C12N15/1075 , B01J19/0046 , C12N15/1006 , C40B40/08 , C40B50/06
摘要： A method of preparing a nucleic acid library in droplets in contact with oil, including: (a) blunt-ending nucleic acid fragments in a droplet in the oil to yield blunt-ended nucleic acid fragments; (b) phosphorylating the blunt-ended nucleic acid fragments in a droplet in the oil to yield phosphorylated nucleic acid fragments; coupling A-tails to the phosphorylated nucleic acid fragments in a droplet in the oil to yield A-tailed nucleic acid fragments; and (d) coupling nucleic acid adapters to the A-tailed nucleic acid fragments in a droplet in the oil to yield the nucleic acid library comprising adapter-ligated nucleic acid fragments.
摘要翻译： 制备与油接触的液滴中的核酸文库的方法，所述方法包括：（a）使油中的液滴中的核酸片段平端化以产生平末端的核酸片段; （b）使油中液滴中的平端核酸片段磷酸化以产生磷酸化的核酸片段; 将A尾连接至油中液滴中的磷酸化核酸片段以产生A尾加核酸片段; （d）将核酸衔接子与油中液滴中的A尾加核酸片段偶联以产生包含衔接子连接的核酸片段的核酸文库。
-
公开(公告)号：EP2914736A4
公开(公告)日：2016-08-31
申请号：EP13852305
申请日：2013-10-30
CPC分类号： C12Q1/32 , G01N33/6893 , G01N2333/90206 , G01N2800/04 , G01N2800/7085
-
23.发明公开TECHNIQUES AND DROPLET ACTUATOR DESIGNS FOR REDUCING BUBBLE FORMATION 审中-公开VERFAHREN UND TROPFENAKTUATORKONZEPTE ZUR REDUZIERUNG EINERBLÄSCHENBILDUNG
公开(公告)号：EP2867645A4
公开(公告)日：2016-06-29
申请号：EP13808882
申请日：2013-06-27
发明人： DELATTRE CYRIL , RIVAL ARNAUD , SRINIVASAN VIJAY
CPC分类号： B01L3/502784 , B01L3/502715 , B01L3/502723 , B01L3/50273 , B01L3/5029 , B01L3/52 , B01L2200/06 , B01L2200/0605 , B01L2300/0645 , B01L2300/0816 , B01L2300/088 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0409 , B01L2400/0415 , B01L2400/0427 , B01L2400/043 , B01L2400/0442 , B01L2400/0448
摘要： During droplet operations in a droplet actuator, bubbles often form in the filler fluid in the droplet operations gap and interrupt droplet operations. The present invention provides methods and systems for performing droplet operations on a droplet in a droplet actuator comprising maintaining substantially consistent contact between the droplet and an electrical ground while conducting multiple droplet operations on the droplet in the droplet operations gap and/or reducing the accumulation of electrical charges in the droplet operations gap during multiple droplet operations. The methods and systems reduce or eliminate bubble formation in the filler fluid of the droplet operations gap, thereby permitting completion of multiple droplet operations without interruption by bubble formation in the filler fluid in the droplet operations gap.
摘要翻译： 在液滴致动器中的液滴操作期间，在液滴操作间隙中，气泡通常在填充液中形成并且中断液滴操作。 本发明提供了用于在液滴致动器中的液滴上进行液滴操作的方法和系统，包括在液滴操作间隙内对液滴进行多个液滴操作并且/或减少 在多个液滴操作期间液滴操作间隙中的电荷。 所述方法和系统减少或消除了液滴操作间隙的填充流体中的气泡形成，从而允许完成多个液滴操作而不会由于液滴操作间隙中的填充流体中的气泡形成而中断。
-
公开(公告)号：EP2188059B1
公开(公告)日：2016-05-04
申请号：EP08828680.2
申请日：2008-08-25
发明人： THWAR, Prasanna , ALLEN, Dwayne , PAIK, Philip
CPC分类号： B01L3/52 , B01L3/502761 , B01L3/502792 , B01L2200/0668 , B01L2300/0864 , B01L2300/089 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427
-
25.发明公开TECHNIQUES AND DROPLET ACTUATOR DESIGNS FOR REDUCING BUBBLE FORMATION 审中-公开气泡，教育方法和TROPFENAKTUATORKONZEPTE还原
公开(公告)号：EP2867645A1
公开(公告)日：2015-05-06
申请号：EP13808882.8
申请日：2013-06-27
CPC分类号： B01L3/502784 , B01L3/502715 , B01L3/502723 , B01L3/50273 , B01L3/5029 , B01L3/52 , B01L2200/06 , B01L2200/0605 , B01L2300/0645 , B01L2300/0816 , B01L2300/088 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0409 , B01L2400/0415 , B01L2400/0427 , B01L2400/043 , B01L2400/0442 , B01L2400/0448
摘要： During droplet operations in a droplet actuator, bubbles often form in the filler fluid in the droplet operations gap and interrupt droplet operations. The present invention provides methods and systems for performing droplet operations on a droplet in a droplet actuator comprising maintaining substantially consistent contact between the droplet and an electrical ground while conducting multiple droplet operations on the droplet in the droplet operations gap and/or reducing the accumulation of electrical charges in the droplet operations gap during multiple droplet operations. The methods and systems reduce or eliminate bubble formation in the filler fluid of the droplet operations gap, thereby permitting completion of multiple droplet operations without interruption by bubble formation in the filler fluid in the droplet operations gap.
-
公开(公告)号：EP2707724A4
公开(公告)日：2015-01-21
申请号：EP12781874
申请日：2012-05-09
发明人： GRAHAM CARRIE , ECKHARDT ALAN , PERKINS LISA
CPC分类号： G01N1/405 , C12N9/16 , C12N9/2402 , C12N9/2408 , C12N9/2471 , C12Y301/06013 , C12Y302/01023 , C12Y302/01076 , G01N33/573 , G01N2333/924 , G01N2333/928 , G01N2333/938 , G01N2800/38 , G06F19/10
-
公开(公告)号：EP2707131A4
公开(公告)日：2014-11-19
申请号：EP12781709
申请日：2012-05-08
发明人： STURMER RYAN , SRINIVASAN VIJAY , SUDARSAN ARJUN
CPC分类号： B01L3/50273 , B01L3/502792 , B01L2200/0621 , B01L2200/10 , B01L2200/14 , B01L2200/16 , B01L2300/0645 , B01L2300/0816 , B01L2300/0829 , B01L2300/087 , B01L2300/089 , B01L2400/0427 , G01N27/048 , G01R27/16
摘要： A method, comprising: generating an output voltage by a power supply; storing charge produced by the output voltage; superimposing an excitation signal onto the output voltage to produce a superimposed signal; connecting the superimposed signal to an electrode in a droplet actuator; suppressing the output voltage from the power supply when detecting an impedance at the electrode; supplying the charge to the droplet actuator to activate the electrode during the impedance; and measuring the impedance produced by the excitation signal while the output voltage is suppressed, wherein the impedance indicates presence of liquid at the electrode. Superimposing the excitation signal may comprise adding the excitation signal to the output voltage. Suppressing the output voltage may comprise at least one of stopping the output voltage generated by the power supply, disabling a switching action of the power supply, and disabling the power supply during the impedance measurement.
-
公开(公告)号：EP2588322A2
公开(公告)日：2013-05-08
申请号：EP11810072.6
申请日：2011-06-24
CPC分类号： B01L3/0241 , B01L3/502784 , B41J2/14024
摘要： The invention provides droplet actuator assemblies and systems and methods of manufacturing the droplet actuator assemblies. In certain embodiments, two-piece enclosures are used to form a droplet actuator assembly that houses a droplet operations substrate. In certain other embodiments, one-piece enclosures are used to form a droplet actuator assembly that houses a droplet operations substrate. In the plastic injection molding process for forming substrates of the droplet actuator assemblies of the present invention may utilize insert molding (or overmolding) processes for forming a gasket in at least one substrate, thereby avoiding the need for providing and installing a separate gasket component. Further, the droplet actuator assemblies may include features that allow ultrasonic welding processes to be used for bonding substrates together. The manufacturing systems of the present invention for fabricating the droplet actuator assemblies may utilize continuous flow reel-to-reel manufacturing processes.
-
公开(公告)号：EP2016189A4
公开(公告)日：2010-12-08
申请号：EP06847588
申请日：2006-12-11
CPC分类号： B01L3/502792 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/502761 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , C12Q1/6869 , G01N33/49 , C12Q2565/629 , C12Q2565/301
-
30.发明公开DROPLET ACTUATOR DEVICES, CONFIGURATIONS, AND METHODS FOR IMPROVING ABSORBANCE DETECTION 有权TROPFENAKTUATORVORRICHTUNGEN，配置和方法吸收好转检测
公开(公告)号：EP2122327A4
公开(公告)日：2010-06-16
申请号：EP08743832
申请日：2008-03-13
CPC分类号： G01N21/0303 , G01N21/031 , G01N2021/035 , G01N2021/036 , G01N2201/08
-
-
-
-
-
-
-
-
-
搜索结果
104 条记录 (耗时 0.02 秒)
