利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

35 条记录 (耗时 0.016 秒)

    GENETIC INCORPORATION OF UNNATURAL AMINO ACIDS INTO PROTEINS IN MAMMALIAN CELLS
    22.
    发明公开
    GENETIC INCORPORATION OF UNNATURAL AMINO ACIDS INTO PROTEINS IN MAMMALIAN CELLS 审中-公开
    遗传安装非天然氨基酸插入在哺乳动物细胞中的蛋白质

    公开(公告)号：EP2069481A4

    公开(公告)日：2010-01-06

    申请号：EP07870795

    申请日：2007-10-17

    申请人： SCRIPPS RESEARCH INST

    发明人： LIU WENSHE ,  SCHULTZ PETER G

    IPC分类号： C12N5/10 ,  C12N5/16 ,  C12N5/18 ,  C12P21/04

    CPC分类号： C12N9/93 ,  C12P21/02

    摘要： The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate unnatural amino acids into proteins in mammalian host cells, for example, primate host cells and rodent host cells. The invention provides, for example but not limited to, translation systems that include host cells (e.g., primate or rodent cells), orthogonal aminoacyl-tRNA synthetases derived from eubacterial synthetases, orthogonal tRNAs, and the unnatural amino acid. The invention also relates to methods for producing proteins of interest comprising at least one unnatural amino acid in mammalian host cell systems.

    SITE SPECIFIC INCORPORATION OF KETO AMINO ACIDS INTO PROTEINS
    27.
    发明公开
    SITE SPECIFIC INCORPORATION OF KETO AMINO ACIDS INTO PROTEINS 有权
    蛋白质中的维生素EINBAU VONKETOAMINOSÄUREN

    公开(公告)号：EP1578949A4

    公开(公告)日：2006-12-20

    申请号：EP03809019

    申请日：2003-10-15

    申请人： SCRIPPS RESEARCH INST

    发明人： SCHULTZ PETER G ,  WANG LEI

    IPC分类号： C07K14/47 ,  C12N9/00 ,  C12N15/10 ,  C12N15/67 ,  C12P21/00 ,  C12P21/02

    CPC分类号： C12N15/67 ,  C12N9/93 ,  C12N15/10 ,  C12P21/005

    摘要： Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate keto amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with keto amino acids using these orthogonal pairs.

    摘要翻译： 提供了将酮基氨基酸掺入蛋白质的蛋白质生物合成机理组分的组成和方法，其中包括正交的tRNAs，正交氨酰-tRNA合成酶和tRNA /合成酶的正交对。 还提供用于鉴定这些正交对的方法以及使用这些正交对产生具有酮基氨基酸的蛋白质的方法。