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    FERMENTATION PROCESS
    25.
    发明公开
    FERMENTATION PROCESS 有权
    GÄRUNGSPROZESS

    公开(公告)号：EP2809343A1

    公开(公告)日：2014-12-10

    申请号：EP13701484.1

    申请日：2013-01-30

    申请人： GlaxoSmithKline Biologicals S.A.

    发明人： DEHOTTAY, Philippe Marc Helene ,  GOFFIN, Philippe

    IPC分类号： A61K39/00

    CPC分类号： C07K14/235 ,  C12N1/20 ,  C12N1/38

    摘要： The present invention relates to a process for fermenting Bordetella comprising a) providing a sample of bacteria of a Bordetella species; b) incubating the sample of bacteria of a Bordetella species in a first environment under at least one bvg (Bordetella virulence genes) modulating condition for at least 5 generations, thereby producing a mature culture; c) incubating the mature culture in a second environment in the absence of the at least one bvg modulating condition; wherein step c) occurs after step b).

    摘要翻译： 一种用于发酵博德氏菌属物种的方法，包括在至少一种bvg（博德特氏菌毒力基因）调节条件下，在第一环境中培养博德特氏菌属物种样品至少5代，以产生成熟培养物，然后孵育成熟 在没有至少一种bvg调节条件的情况下在第二环境中培养。

    INDUCABLE EXPRESSION FROM THE EUKARYOTIC POL -2 PROMOTER IN PROKARYOTES
    29.
    发明公开
    INDUCABLE EXPRESSION FROM THE EUKARYOTIC POL -2 PROMOTER IN PROKARYOTES 有权
    基因诱导表达来自原核生物基因真核POL-2启动子

    公开(公告)号：EP2742127A1

    公开(公告)日：2014-06-18

    申请号：EP12716732.8

    申请日：2012-02-21

    申请人： MELLO BIOTECHNOLOGY, INC.

    发明人： LIN, Shi-Lung ,  CHANG, Donald C.

    IPC分类号： C12N1/38

    CPC分类号： C12N15/635 ,  C12N1/38 ,  C12N15/70

    摘要： Eukaryotic protein-coding messenger RNAs and non-coding microRNAs are naturally transcribed by type II RNA polymerases (pol-2) but not prokaryotic RNA polymerases. As a result, current eukaryotic RNA and protein production is performed either using eukaryotic pol-2 promoters in hybridomas or mammalian cells or using prokaryotic promoters in bacterial cells. However, because prokaryotic RNA transcription tends to be error-prone, frequent mutation is a big problem. Also, growing hybridomas or mammalian cells is relatively laborious and costly. To overcome these problems, the present invention provides a novel inducible composition and method for producing eukaryotic RNAs and/or their related peptides/proteins directly using eukaryotic pol-2 promoter-driven gene expression in fast growing bacteria, without the need of changing to prokaryotic promoters or growing hybridomas/mammalian cells. The RNAs and peptides/proteins so obtained can be used to develop drugs, cure diseases, treat tumors/cancers, produce pluripotent stem (iPS) cells, enhance wound healing, and make foods.