公开(公告)号：EP0716695A1

公开(公告)日：1996-06-19

申请号：EP94926507.0

申请日：1994-08-11

申请人： THE SALK INSTITUTE BIOTECHNOLOGY/INDUSTRIAL ASSOCIATES, INC.

发明人： HARPOLD, Michael, M. ,  ELLIS, Steven, B. ,  WILLIAMS, Mark, E. ,  McCUE, Ann, F. ,  GILLESPIE, Alison

IPC分类号： G01N33 ,  C07K14 ,  C07K16 ,  C12N1 ,  C12N5 ,  C12N15 ,  C12P21 ,  C12Q1 ,  C12R1

CPC分类号： G01N33/6872 ,  C07K14/705 ,  C12Q1/6876 ,  C12Q2600/158 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5091

摘要： Isolated DNA encoding each of human calcium chanel α1-, α2-, β- and η-subunits, including subunits that arise as splice variants of primary transcripts, is provided. In particular DNA clones encoding each of the α1A-1, α1A-2, α1E-1, α1C-2, α1E-3, β3-1, β2C, β2D, β2E and β4 subunits of human calcium channels are provided. Cells and vectors containing the DNA, subunit specific antibodies and nucleic acid probes and methods for identifying compounds that modulate the activity of human calcium channels are also provided.

公开(公告)号：EP0708917A1

公开(公告)日：1996-05-01

申请号：EP94923731.0

申请日：1994-07-12

申请人： EUROPEAN ECONOMIC COMMUNITY represented by THE COMMISSION OF THE EUROPEAN COMMUNITIES

发明人： STROOSNIJDER, Marinus, Frederik ,  MACCHI, Giovanni, Michele

IPC分类号： G01N3 ,  G01N1 ,  G01N33

CPC分类号： G01N3/60 ,  G01N3/562 ,  G01N33/388 ,  G01N2001/2833 ,  G01N2203/0224 ,  Y10T436/13

摘要： A method and apparatus are described for quantative determination of the level of spalling of a protective surface coating as a result of repetitive heating and cooling. The method comprises introducing at least one radionuclide into the protective coating of an article (16), alternately heating and cooling the article (16) for a predetermined number of heating and cooling cycles, collecting the spalled particles (10) and measuring the radioactivity thereof. The apparatus comprises a chamber (2) for receiving the coated article, means (4) for heating the chamber (2) or the article (16) and a spall collector (8). The spall collector (8) is characterized in having means for immobilizing any spalled coating particles (10) and in being so adapted that the level of radioactivity of any spalled particles can be measured by a η-spectrometer.

公开(公告)号：EP0705344A1

公开(公告)日：1996-04-10

申请号：EP94920351.0

申请日：1994-06-24

申请人： McMASTER UNIVERSITY

发明人： GRAHAM, Frank, L. ,  BETT, Andrew ,  PREVEK, Ludvik ,  HADDARA, Wael

IPC分类号： C12N15 ,  C12N7 ,  C12P21

CPC分类号： C12N15/86 ,  A01K2217/05 ,  C12N2710/10343 ,  C12N2800/30

摘要： The invention comprises a series of adenovirus-based vectors having deletions in the E1 and/or E3 regions, and optionally insertions of pBR322 sequences, which can be used to deliver nucleic acid inserts into host cells, tissues or organisms that then can express the insert. The invention also comprises the use of these vectors in introducing genes into cells, in making vaccines and in gene therapy.

公开(公告)号：EP0698118A1

公开(公告)日：1996-02-28

申请号：EP95913592.0

申请日：1995-03-07

申请人： BIOGENEX LABORATORIES

发明人： ZHANG, Guangrong ,  YU, Cheng-Zhi ,  SU, Sheng-Hui ,  KALRA, Krishan, L. ,  ZHOU, Ding

IPC分类号： G01N33 ,  C08L91 ,  G01N1

CPC分类号： C11D1/143 ,  C11D1/62 ,  C11D1/72 ,  C11D3/187 ,  C11D3/188 ,  C11D3/201 ,  C11D3/2044 ,  G01N1/30 ,  G01N1/36 ,  Y10S435/975

摘要： Compositions and methods are provided for dewaxing wax-embedded biological specimens prior to histochemical analysis. The compositions and methods provided can effectively remove wax or improved wax-based embedding materials, particularly paraffin-based, from specimens during preparation for histochemical or other diagnostic analyses, while minimizing danger to users, achieving compatibility with automated use, and maintaining compatibility with downstream histochemical analyses, particularly immunostaining. Compositions of the invention comprise a paraffin-solubilizing organic solvent, a polar organic solvent, and a surfactant. Compositions can further comprise water. The method involves contacting a wax-embedded specimen with the dewaxing composition to solubilize the wax impregnating the specimen prior to histochemical analysis. The method can comprise the further step of washing the dewaxed specimen immediately after dewaxing with an aqueous wash composition comprising a detergent to remove residual dewaxing composition. Also provided is a kit for dewaxing a wax-embedded specimen, which comprises a dewaxing composition and can further comprise a second composition of (1) an immunostaining reagent or (2) an aqueous wash solution comprising a detergent for removing residual dewaxing solution.

公开(公告)号：EP0696228A1

公开(公告)日：1996-02-14

申请号：EP94913770.0

申请日：1994-05-05

申请人： EA TECHNOLOGY LIMITED

发明人： GILROY, Daniel ,  CAMPBELL, Duncan Alistair

IPC分类号： B01D53 ,  C01B21 ,  C25B1

CPC分类号： C25B1/24 ,  B01D53/326 ,  B01D53/58 ,  C01B21/02 ,  Y02A50/2346

摘要： PCT No. PCT/GB94/00958 Sec. 371 Date Mar. 21, 1996 Sec. 102(e) Date Mar. 21, 1996 PCT Filed May 5, 1994 PCT Pub. No. WO94/26395 PCT Pub. Date Nov. 24, 1994A process for converting ammonia in a gas stream to nitrogen comprises contacting the gas stream with an electrolyte containing bromide and hypobromite ions. The ammonia is dissolved and oxidized by the hypobromite ions to nitrogen. Thereafter the electrolyte is passed through an electrochemical cell containing an anode and a cathode to regenerate the hyperbromite ions by the action of an electric current flowing across the cell. The conversion of ammonia to nitrogen in accordance with the invention is adventageous as nitrogen may be disposed of without harm to the environment whilst ammonia has toxic effects.

公开(公告)号：EP0681477A1

公开(公告)日：1995-11-15

申请号：EP94907366.0

申请日：1994-01-28

申请人： NEUROSPHERES LTD.

发明人： WEISS, Samuel ,  REYNOLDS, Brent A. ,  HAMMANG, Joseph P. ,  BAETGE, Edward E.

IPC分类号： A61K ,  C12N ,  C12N15 ,  A61K31 ,  A61K35 ,  A61K38 ,  A61K48 ,  A61P25 ,  C07H21 ,  C07K14 ,  C12N5 ,  C12N9 ,  C12P21

CPC分类号： C12N5/0623 ,  A01K2217/05 ,  A61K35/30 ,  A61K38/179 ,  A61K48/00 ,  C07K14/47 ,  C07K14/475 ,  C07K14/82 ,  C12N9/0065 ,  C12N9/2471 ,  C12N2500/25 ,  C12N2500/46 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/148 ,  C12N2501/392 ,  C12N2510/00 ,  C12Y302/01023 ,  A61K2300/00

摘要： Genetically modified, epidermal growth factor-responsive neural stem cells are disclosed that are capable of differentiating into neurons, astrocytes, and oligodendrocytes in vitro. The stem cells can be proliferated in vitro to result in large numbers of cells for use in transplantation to treat various neurological disorders. The stem cells can be obtained from a human or other animal, cultured in vitro in the presence of a growth factor and genetically modified with desired DNA using techniques known in the art. Alternatively, the precursor cells can be derived from a transgenic animal which has had a desired gene inserted into its genome.

公开(公告)号：EP0673252A1

公开(公告)日：1995-09-27

申请号：EP94904446.0

申请日：1993-12-14

申请人： START TECHNOLOGY PARTNERSHIP

发明人： WEISS, Benjamin

IPC分类号： G01N33 ,  A61K31 ,  A61P25 ,  C07H21 ,  C12N15 ,  C12Q1

CPC分类号： A61K31/70 ,  C07H21/00 ,  C12N15/1138

摘要： A method and pharmaceutical preparation are provided for diagnosing and treating pathological conditions related to a dopamine receptor abnormality. The method comprises administering to a patient having such a pathological condition an oligonucleotide antisense to one or more RNA molecules encoding one of the several dopamine receptors. The antisense oligonucleotides bind specifically to expression-controlling sequences of such RNA molecules, thereby selectively controlling expression of one or more dopamine receptor subtypes, and alleviating the pathological conditions related to their expression.

公开(公告)号：EP0669973A1

公开(公告)日：1995-09-06

申请号：EP93923994.0

申请日：1993-10-27

申请人： NEUROSPHERES LTD.

发明人： WEISS, Samuel ,  REYNOLDS, Brent, A. ,  HAMMANG, Joseph, P. ,  BAETGE, E. Edward

IPC分类号： A61K38 ,  C07K14 ,  C12N5 ,  C12N9 ,  A61K35 ,  A61K48

CPC分类号： C07K14/82 ,  A01K2217/05 ,  A61K48/00 ,  C07K14/47 ,  C07K14/475 ,  C12N5/0623 ,  C12N9/0065 ,  C12N9/88 ,  C12N2500/25 ,  C12N2500/46 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/148 ,  C12N2501/392 ,  C12N2510/00 ,  C12Y402/01011

摘要： Methods are described for increasing the number of neural stem cells that differentiate into astrocytes, oligodendrocytes, or neurons. The methods comprise proliferating isolated neural stem cells in a culture medium having a first growth factor to produce precursor cells. The precursor cells are then differentiated into astrocytes, oligodendrocytes, or neurons in a second culture medium, free of the first growth factor, containing a second growth factor or combination of growth factors.

公开(公告)号：EP0664832A1

公开(公告)日：1995-08-02

申请号：EP93922482.0

申请日：1993-10-15

申请人： NEUROSPHERES LTD.

发明人： WEISS, Samuel ,  REYNOLDS, Brent, A. ,  HAMMANG, Joseph, P.

IPC分类号： A61K35 ,  A61K38 ,  A61P25 ,  C07K14 ,  C12N5 ,  C12N9 ,  A61K48

CPC分类号： A61K35/30 ,  A01K2217/05 ,  A61K48/00 ,  C07K14/47 ,  C07K14/475 ,  C07K14/82 ,  C12N5/0623 ,  C12N9/0065 ,  C12N2500/25 ,  C12N2500/46 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/392

摘要： A method for the remyelination of neurons is disclosed wherein neural stem cells isolated from adult or fetal neural tissue are proliferated in a culture medium containing a growth factor to produce precursor cells having a nestin (+) phenotype. The precursor cells are capable of differentiation into oligodendrocytes which, when associated with a demyelinated neuron, effect remyelination.

公开(公告)号：EP0662150A1

公开(公告)日：1995-07-12

申请号：EP93921337.0

申请日：1993-09-01

申请人： DANA-FARBER CANCER INSTITUTE, INC.

发明人： SAGER, Ruth

IPC分类号： G01N33 ,  A61K31 ,  A61K39 ,  A61K48 ,  A61P35 ,  C07H21 ,  C07K14 ,  C07K16 ,  C07K19 ,  C12N5 ,  C12N15 ,  C12P21 ,  C12Q1 ,  A61K38

CPC分类号： C12Q1/6841 ,  A61K38/00 ,  C07K14/811 ,  C07K16/38 ,  C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/136

摘要： An isolated DNA encoding a polypeptide substantially identical to maspin (SEQ ID NO: 1); a substantially purified preparation of maspin; an antibody specific for maspin; and use of such DNAs and antibodies in diagnostic, screening, and therapeutic methods.