公开(公告)号：EP2846126B1

公开(公告)日：2015-09-16

申请号：EP13183033.3

申请日：2013-09-04

申请人： Bogen Electronic GmbH

发明人： Hoyer, Jörn ,  Becker, Torsten

IPC分类号： G01B5/24 ,  G01D13/16

CPC分类号： G01D5/14 ,  G01D5/145 ,  G01D5/2497 ,  G01D13/16

公开(公告)号：EP2851730A1

公开(公告)日：2015-03-25

申请号：EP13184931.7

申请日：2013-09-18

申请人： Hochschule Aalen

发明人： Bruns, Thomas ,  Schneckenburger, Herbert ,  Schickinger, Sarah

IPC分类号： G02B21/00 ,  G02B21/26 ,  G02B21/33 ,  G02B21/36 ,  G02B21/16 ,  G02B21/34

CPC分类号： G02B21/0004 ,  G02B21/16 ,  G02B21/26 ,  G02B21/33 ,  G02B21/34 ,  G02B21/367

摘要： The invention relates to a sample holder (1) for a microscope comprising a transparent outer reservoir (2) and a transparent inner tube (3), which is arranged in the outer reservoir (2) to be rotatable. The invention further relates to a method for observing a specimen (23), comprising placing a specimen (23) in an inner tube (3), placing the inner tube (3) in an outer reservoir (2), dispersing an immersion fluid (20), which has a refraction index corresponding to the refraction index of the inner tube (3), between the inner tube (3) and the outer reservoir (2), locating the outer reservoir (2) in the light path of a microscope, and rotating the inner tube (3) to observe the specimen (23) at any desired perspective. Additionally, the invention relates to a microscope stage adaptor and to the use of a sample holder (1) for obtaining images.

摘要翻译： 本发明涉及一种用于显微镜的样品保持器（1），其包括透明的外储存器（2）和透明内管（3），该透明内管（3）布置在外储存器（2）中以可旋转。 本发明还涉及一种用于观察试样（23）的方法，包括将试样（23）放置在内管（3）中，将内管（3）放置在外储存器（2）中，分散浸液（ 20），其具有与内管（3）的折射率相对应的折射指数，位于内管（3）和外储存器（2）之间，将外储存器（2）定位在显微镜的光路中 ，并且以任何期望的角度旋转内管（3）以观察样品（23）。 另外，本发明涉及显微镜台适配器以及用于获得图像的样品架（1）的使用。

公开(公告)号：EP2824456A1

公开(公告)日：2015-01-14

申请号：EP13175775.9

申请日：2013-07-09

申请人： Universität Konstanz

发明人： Nikolay, Rainer, Dr. ,  Deuerling, Elke, Prof. Dr.

IPC分类号： G01N33/542 ,  G01N33/58 ,  G01N33/569 ,  C07K14/195 ,  C12N15/10 ,  C12Q1/18

CPC分类号： C12N15/1041 ,  C07K14/195 ,  C07K14/43595 ,  C07K2319/60 ,  C12Q1/18 ,  G01N33/542

摘要： The invention relates to a method and a prokaryotic cell for identifying a compound, which interferes with ribosome biogenesis, assembly and/or degradation. The cell expresses a first fusion protein comprising a protein of a small ribosomal subunit, an amino acid linker and a first fluorescent protein, and a second fusion protein comprising a protein of a large ribosomal subunit, an amino acid linker and a second fluorescent protein. The invention further relates to a gene construct comprising a first element encoding a protein of a small ribosomal subunit fused to a first fluorescent protein by an amino acid linker, and a second element encoding a protein of a large subunit of a ribosome fused to a second fluorescent protein by an amino acid linker.

摘要翻译： 本发明涉及用于鉴定干扰核糖体生物发生，组装和/或降解的化合物的方法和原核细胞。 细胞表达包含小核糖体亚基，氨基酸接头和第一荧光蛋白的蛋白质的第一融合蛋白，以及包含大核糖体亚基，氨基酸接头和第二荧光蛋白的蛋白质的第二融合蛋白。 本发明还涉及一种基因构建体，其包含编码通过氨基酸接头与第一荧光蛋白融合的小核糖体亚基的蛋白质的第一元件，以及编码与第二元件融合的核糖体的大亚基的蛋白质的第二元件 荧光蛋白通过氨基酸接头。

公开(公告)号：EP2706120A1

公开(公告)日：2014-03-12

申请号：EP12183209.1

申请日：2012-09-05

申请人： Karlsruher Institut für Technologie

发明人： Buecking, Clemens ,  Goltsch, Frederik ,  Gescher, Johannes

IPC分类号： C12Q1/00 ,  C12Q1/26 ,  C12N9/00 ,  C12N15/00 ,  G01N27/00

CPC分类号： C12Q1/005 ,  C07K14/80 ,  C12Q1/26 ,  G01N33/0047

摘要： The invention relates to a nucleic acid construct for detecting a compound comprising a coding region and a regulatory element, which controls expression of the coding region depending on the compound and to a cell comprising such a construct. In addition, the invention relates to a method for detecting a compound in a sample. The invention further relates to a biosensor for detecting a compound comprising an electrode system, a device for measuring electron transfer in the electrode system, and a microorganism.

摘要翻译： 本发明涉及用于检测包含编码区和调节元件的化合物的核酸构建体，其控制取决于化合物的编码区的表达和包含这种构建体的细胞。 此外，本发明涉及一种检测样品中化合物的方法。 本发明还涉及用于检测包括电极系统的化合物的生物传感器，用于测量电极系统中的电子转移的装置和微生物。

公开(公告)号：EP2691529A1

公开(公告)日：2014-02-05

申请号：EP12763234.7

申请日：2012-04-02

申请人： University of Iowa Research Foundation

发明人： DAVIDSON, Beverly, L.

IPC分类号： C12N15/86

CPC分类号： A61K48/0075 ,  C12N9/485 ,  C12N15/86 ,  C12N2799/025

摘要： The present disclosure provides methods of treating a disease in a non-rodent mammal comprising administering to the cerebrospinal fluid (CSF) of the non-rodent mammal an rAAV2 particle containing a vector comprising a nucleic acid encoding a therapeutic protein inserted between a pair of AAV inverted terminal repeats in a manner effective to infect an ependymal cell in the non-rodent mammal, wherein the ependymal cell secretes the therapeutic protein so as to treat the disease.

摘要翻译： 本公开内容提供了治疗非啮齿动物哺乳动物中的疾病的方法，包括向非啮齿动物哺乳动物的脑脊液（CSF）施用含有载体​​的rAAV2颗粒，所述载体包含编码插入一对AAV之间的治疗性蛋白质的核酸 以有效感染非啮齿动物哺乳动物的室管膜细胞的方式进行反向末端重复，其中室管膜细胞分泌治疗性蛋白质以治疗疾病。

公开(公告)号：EP2358871B1

公开(公告)日：2013-12-25

申请号：EP09760864.0

申请日：2009-11-26

申请人： Julius-Maximilians-Universität Würzburg

发明人： LOHSE, Martin J. ,  LORENZ, Kristina

IPC分类号： C12N9/12 ,  C07K16/40 ,  C12N15/54 ,  G01N33/573 ,  G01N33/68

CPC分类号： C07K16/40 ,  A01K67/0275 ,  A01K2217/052 ,  A01K2217/056 ,  A01K2227/105 ,  A01K2267/0375 ,  C07K16/44 ,  C12N9/1205 ,  G01N33/573 ,  G01N33/6893 ,  G01N2800/32

摘要： The invention relates to an antibody specific to Erk1/2 phosphorylated at Thr188 and a method for producing the same. The invention also relates to an in vitro method for determining the presence of phosphorylated Erk1/2 in a sample using the antibody of the invention. Therefore, the invention also comprises an assay for diagnosing a heart disease in vitro comprising the antibody of the invention and certain uses of the antibody. Moreover the invention relates to a peptide used to produce the antibody.

公开(公告)号：EP2471925A1

公开(公告)日：2012-07-04

申请号：EP12162435.7

申请日：2008-03-22

申请人： Yale University

发明人： Breaker, Ronald ,  Sudarsan, Narasimhan ,  Wachter, Andreas ,  Cheah, Ming Tatt

IPC分类号： C12N15/11 ,  C12N15/115 ,  A01N43/78 ,  A61K31/7088

CPC分类号： C12N15/111 ,  C12N15/115 ,  C12N15/63 ,  C12N2310/16 ,  C12N2310/3519 ,  C12N2320/33

摘要： Disclosed are methods and compositions related to riboswitches that control alternative splicing.

摘要翻译： 公开了一种方法和相关的核糖开关的组合物没有控制可变剪接。

公开(公告)号：EP2467131A2

公开(公告)日：2012-06-27

申请号：EP10810535.4

申请日：2010-08-18

申请人： University of Florida Research Foundation, Inc. ,  The Regents of the University of Colorado, A Body Corporate

发明人： KEM, William, Reade ,  SOTI, Ferenc ,  FREEDMAN, Robert ,  JOHNSON, Lynn

IPC分类号： A61K9/22 ,  A61K9/20 ,  A61K47/30 ,  A61K31/444 ,  A61K31/44

CPC分类号： A61K9/4866 ,  A61K31/444

摘要： The subject invention pertains to controlled-release dosage forms of anabaseine compounds, such as 3-(2,4-dimethoxybenzylidene)-anabaseine (also known as DMXBA or GTS-21) or a pharmaceutically acceptable salt thereof, methods of use, and methods for producing controlled-release dosage forms.

公开(公告)号：EP2438184A1

公开(公告)日：2012-04-11

申请号：EP10782824.6

申请日：2010-06-02

申请人： Monoquant PTY LTD ,  Flinders University

发明人： MORLEY, Alexander, Alan ,  BRISCO, Michel Julian

IPC分类号： C12P19/34

CPC分类号： C12Q1/686 ,  C12Q2549/119 ,  C12Q2527/107

摘要： The present invention relates generally to a method of amplifying a nucleic acid region of interest and, more particularly, to a method of amplifying a nucleic acid region of interest via a nested single tube PCR. The method of the invention is designed to provide a means to selectively inactivate the functionality of the outer primer or primers via an antisense nucleic acid molecule targeted to at least one of the outer primers, thereby maintaining amplification efficiency throughout the reaction. The development of a means to achieve efficient amplification by the outer primer followed by efficient amplification with the inner primers, in the context of a single tube nested PCR, is useful in a range of applications including, but not limited to, the diagnosis and/or monitoring of disease conditions which are characterised by specific gene sequences and the characterisation or analysis of specific gene regions of interest. Still further, the method of the present invention enables quantification to be performed and not just simple detection.

公开(公告)号：EP2295502A1

公开(公告)日：2011-03-16

申请号：EP09168292.2

申请日：2009-08-20

申请人： Ruprecht-Karls-Universität Heidelberg

发明人： Kraemer, Roland, Prof. Dr. ,  Szelke, Helga, Dr. ,  Harenberg, Job, Prof. Dr. ,  Poeck, Armin

IPC分类号： C09B5/62

CPC分类号： C09B5/62 ,  Y10T436/143333

摘要： The invention relates to a perylene diimide derivative and to an in vitro method for preparing the same. In addition, the invention relates to a method for detecting heparin in a sample using perylene diimide derivatives, wherein the interaction of the perylene diimide derivative with heparin modulates the intensity of the fluorescent signal of the mixture compared to the solution. The invention also comprises a diagnostic kit for detecting heparin in a sample in vitro, comprising the perylene diimide derivative, as well as the use of the perylene diimide derivative for neutralizing the anticoagulant activity of heparin.

摘要翻译： 本发明涉及一种苝二酰亚胺衍生物及其制备方法。 此外，本发明涉及使用苝二酰亚胺衍生物检测样品中肝素的方法，其中苝二酰亚胺衍生物与肝素的相互作用与溶液相比调节混合物的荧光信号的强度。 本发明还包括用于在体外检测样品中的肝素的诊断试剂盒，其包含苝二酰亚胺衍生物，以及使用苝二酰亚胺衍生物来中和肝素的抗凝血活性。