公开(公告)号：EP2016091A4

公开(公告)日：2009-09-09

申请号：EP06849949

申请日：2006-12-11

申请人： ADVANCED LIQUID LOGIC INC ,  UNIV DUKE

发明人： POLLACK MICHAEL G ,  PAMULA VAMSEE K ,  SRINIVASAN VIJAY ,  PAIK PHILIP Y ,  ECKHARDT ALLEN E ,  FAIR RICHARD B

IPC分类号： C07K1/26 ,  B41J2/01 ,  C12M1/34 ,  C12Q1/68 ,  G01N27/26

CPC分类号： B01L3/502792 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502715 ,  B01L3/502761 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0668 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  B01L2400/0448 ,  C12Q1/6869 ,  G01N33/49 ,  C12Q2565/629 ,  C12Q2565/301

摘要： The present invention relates to a droplet microactuator and to systems, apparatuses and methods employing the droplet microactuator for executing various protocols using discrete droplets. The invention includes a droplet microactuator or droplet microactuator system having one or more input reservoirs loaded with reagents for conducting biochemical reactions, such as the reagents described for use in nucleic acid amplification protocols, affinity-based assay protocols, sequencing protocols, and protocols for analyses of biological fluids.

摘要翻译： 本发明涉及一种液滴微致动器，并涉及采用液滴微致动器用于使用离散液滴执行各种方案的系统，设备和方法。 本发明包括具有一个或多个装载有用于进行生物化学反应的试剂的输入储存器的液滴微致动器或液滴微致动器系统，诸如用于核酸扩增方案，基于亲和力的测定方案，测序方案和分析方案 的生物流体。

公开(公告)号：EP2021103A2

公开(公告)日：2009-02-11

申请号：EP07867113.8

申请日：2007-05-09

申请人： Advanced Liquid Logic, Inc. ,  Duke University

发明人： PAIK, Philip Y. ,  BRAFFORD, Keith R. ,  STURMER, Ryan A. ,  SMITH, Gregory F. ,  POLLACK, Michael G. ,  PAMULA, Vamsee K. ,  WEST, Richard M. ,  SRINIVASAN, Vijay

IPC分类号： B01D57/00

CPC分类号： B01F13/0076 ,  B01F13/0071 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L7/54 ,  B01L2200/0673 ,  B01L2200/14 ,  B01L2200/143 ,  B01L2200/16 ,  B01L2300/027 ,  B01L2300/0819 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0421 ,  B01L2400/0427 ,  B01L2400/0448 ,  G01N33/49 ,  G01N2035/1034

摘要： The present invention relates to systems and methods for controlling droplet movements on a droplet microactuator and a droplet microactuator device, system and method for processing and/or analyzing samples. According to one aspect, a system is provided and includes a controller, a droplet microactuator electronically coupled to the controller, and a display device displaying a user interface electronically coupled to the controller, wherein the system is programmed and configured to permit a user to effect a droplet manipulation by interacting with the user interface. According to another aspect, a sample analyzer is provided and includes an analyzer unit comprising electronic or optical receiving means, a cartridge comprising self-contained droplet handling capabilities, and a means for coupling the cartridge to the analyzer unit which aligns electronic and/or optical outputs from the cartridge with electronic or optical receiving means on the analyzer unit.

摘要翻译： 本发明涉及用于控制液滴微致动器上的液滴运动的系统和方法以及用于处理和/或分析样本的液滴微致动器装置，系统和方法。 根据一个方面，提供了一种系统，该系统包括控制器，电子地耦合到控制器的液滴微致动器，以及显示电子地耦合到控制器的用户界面的显示设备，其中系统被编程并且被配置为允许用户影响 通过与用户界面交互进行液滴操作。 根据另一方面，提供了样本分析仪，并且包括分析仪单元，该分析仪单元包括电子或光学接收装置，包括独立液滴处理能力的盒，以及用于将盒耦合到分析仪单元的装置，该分析仪单元使电子和/或光学 从分析仪单元上带有电子或光学接收装置的盒中输出。

公开(公告)号：EP1999273A1

公开(公告)日：2008-12-10

申请号：EP07735169.0

申请日：2007-03-19

申请人： Koninklijke Philips Electronics N.V.

发明人： PONJEE, Marc, W., G. ,  GILLIES, Murray, F. ,  JOHNSON, Mark, T. ,  FISH, David, A.

IPC分类号： C12Q1/68

CPC分类号： B01L3/50273 ,  B01L3/5027 ,  B01L3/502792 ,  B01L7/52 ,  B01L2200/147 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/1827 ,  B01L2300/1883 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0442 ,  B01L2400/0448

摘要： The invention relates to different designs of a microelectronic device comprising heating electrodes (HE) and field electrodes (FE) that have effect in the same sub-region of a sample chamber. By applying appropriate voltages to the field electrodes (FE), an electrical field (E) can be generated in the sample chamber. By applying appropriate currents to the heating electrodes (HE), the sample chamber can be heated according to a desired temperature profile. The heating electrodes (HE) may optionally be operated as field electrodes such that they generate an electrical field in the sample chamber, too.

公开(公告)号：EP1998886A1

公开(公告)日：2008-12-10

申请号：EP07735141.9

申请日：2007-03-16

申请人： Koninklijke Philips Electronics N.V.

发明人： JOHNSON, Mark, T. ,  FISH, David, A. ,  PONJEE, Marc, W., G.

IPC分类号： B01J19/00

CPC分类号： B01L3/50273 ,  B01L3/5027 ,  B01L3/502792 ,  B01L7/52 ,  B01L2200/147 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/1827 ,  B01L2300/1883 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0442 ,  B01L2400/0448

摘要： The invention relates to different designs of a microelectronic device comprising an array of heating elements (HE) with local driving units (CU2) and optionally with an array of sensor elements (SE) adjacent to a sample chamber (SC). By applying appropriate currents to the heating elements (HE), the sample chamber can be heated according to a desired temperature profile. The local driving units comprise means for compensating variations of their individual characteristics.

公开(公告)号：EP1461454A2

公开(公告)日：2004-09-29

申请号：EP02778182.2

申请日：2002-10-25

申请人： NTU Ventures Pte. Ltd. ,  DSO National Laboratories

发明人： YAP, Peng, Huat, Eric ,  GONG, Haiqing ,  AYI, Teck, Choon

IPC分类号： C12Q1/68

CPC分类号： C12Q1/68 ,  B01L3/5027 ,  B01L7/52 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/044 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2400/0403 ,  B01L2400/0406 ,  B01L2400/0427 ,  B01L2400/0448 ,  B01L2400/0475 ,  B01L2400/0487 ,  B01L2400/06 ,  G01N1/28 ,  Y02A50/54 ,  C12Q2565/629

摘要： The present invention relates to diagnostic methods utilizing an apparatus comprising a substrate having at least one assay station. The at least one assay station has at least a first assay station channel and at least a second assay station channel and the first and second assay station channels each separately being in communication with the at least one assay station. The apparatus has an arrangement of at least first and second multi-purpose channels in communication with the first and second assay station channels, respectively. The first multipurpose channel and first assay station channel have internal surface characteristics conducive to conduction of a sample solution therethrough. There is at least one sample fluid inlet in communication with the at least first multi-purpose channel, and at least one isolation-medium inlet in communication with the at least first and second multi-purpose channels. The at least one second multi-purpose channel has an internal surface portion non-conducive to conduction of said sample solution.

公开(公告)号：EP0946749A1

公开(公告)日：1999-10-06

申请号：EP97947614.0

申请日：1997-11-19

申请人： THE REGENTS OF THE UNIVERSITY OF MICHIGAN ,  Becton, Dickinson and Company

发明人： BURNS, Mark ,  DENUZZIO, John, D. ,  BURKE, David, T. ,  JOHNSON, Brian, N. ,  BEYER, Wayne F., Jr.

IPC分类号： B01J19 ,  B01L3 ,  B01L7 ,  C12Q1 ,  B81B1

CPC分类号： B82Y30/00 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00853 ,  B01J2219/00871 ,  B01J2219/00873 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00912 ,  B01J2219/00952 ,  B01J2219/00961 ,  B01J2219/00986 ,  B01J2219/00995 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/00 ,  B01L7/52 ,  B01L7/54 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0861 ,  B01L2300/0887 ,  B01L2300/163 ,  B01L2300/165 ,  B01L2300/1805 ,  B01L2400/0406 ,  B01L2400/0442 ,  B01L2400/0448 ,  B01L2400/0677 ,  B01L2400/0688 ,  C12Q1/6844

摘要： Disclosed are methods and compositions for isothermal amplification of nucleic acids in a microfabricated substrate. Methods and compositions for the analysis of isothermally amplified nucleic acids in a microfabricated substrate are disclosed as well. The microfabricated substrates and isothermal amplification and detection methods provided are envisioned for use in various diagnostic methods, particularly those connected with diseases characterized by altered gene sequences or gene expression.

公开(公告)号：EP1999272B1

公开(公告)日：2017-11-01

申请号：EP07705980.6

申请日：2007-03-01

申请人： Koninklijke Philips N.V.

发明人： JOHNSON, Mark, T. ,  STAPERT, Hendrik, R. ,  PONJEE, Marc. W., G.

IPC分类号： B01L3/00

CPC分类号： B01L3/50273 ,  B01L3/5027 ,  B01L3/502792 ,  B01L7/52 ,  B01L2200/147 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/1827 ,  B01L2300/1883 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0442 ,  B01L2400/0448

公开(公告)号：EP2021103B1

公开(公告)日：2017-07-12

申请号：EP07867113.8

申请日：2007-05-09

申请人： Advanced Liquid Logic, Inc. ,  Duke University

发明人： PAIK, Philip Y. ,  BRAFFORD, Keith R. ,  STURMER, Ryan A. ,  SMITH, Gregory F. ,  POLLACK, Michael G. ,  PAMULA, Vamsee K. ,  WEST, Richard M. ,  SRINIVASAN, Vijay

IPC分类号： B01D57/00 ,  B01L3/00 ,  G01N35/00

CPC分类号： B01F13/0076 ,  B01F13/0071 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L7/54 ,  B01L2200/0673 ,  B01L2200/14 ,  B01L2200/143 ,  B01L2200/16 ,  B01L2300/027 ,  B01L2300/0819 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0421 ,  B01L2400/0427 ,  B01L2400/0448 ,  G01N33/49 ,  G01N2035/1034

公开(公告)号：EP2895591A4

公开(公告)日：2016-10-12

申请号：EP13837228

申请日：2013-09-12

申请人： GNUBIO INC

发明人： ESMAIL ADNAN ,  HEALY JOHN ,  HUNG TONY ,  KIANI SEPEHR ,  MARY PASCALINE ,  RAZ TAL

IPC分类号： C12M1/00 ,  B01L3/00 ,  B01L7/00 ,  C12M1/36 ,  C12M3/00

CPC分类号： B01L3/502784 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/0673 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/18 ,  B01L2300/1861 ,  B01L2400/0415 ,  B01L2400/0433 ,  B01L2400/0448 ,  B01L2400/088 ,  G01N35/08 ,  Y10T436/143333 ,  Y10T436/2575

摘要： The present invention provides a microfluidic system, method and kit for performing assays. The system may comprise a microfluidic device and a detector, wherein the assay yields results that may be read by a detector and analyzed by the system. The assay may comprise one or more chemical or biological reaction against, or performed on, a sample or multiple samples. The sample(s) may become larger and/or smaller during the performance of the assay. The sample(s) may be present within a vehicle, or on a carrier within a vehicle, in the microfluidic device, and wherein the vehicle may become larger and/or smaller during the performance of the assay. The assay may be a cascading assay comprising a series of multiple assays, wherein each assay may be the same or different, and wherein each assay in the series of multiple assays may further comprise one or more process or step.

公开(公告)号：EP2867645A4

公开(公告)日：2016-06-29

申请号：EP13808882

申请日：2013-06-27

申请人： ADVANCED LIQUID LOGIC INC

发明人： DELATTRE CYRIL ,  RIVAL ARNAUD ,  SRINIVASAN VIJAY

IPC分类号： G01N1/00 ,  B01D57/02 ,  B01L3/00

CPC分类号： B01L3/502784 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/5029 ,  B01L3/52 ,  B01L2200/06 ,  B01L2200/0605 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/088 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0442 ,  B01L2400/0448

摘要： During droplet operations in a droplet actuator, bubbles often form in the filler fluid in the droplet operations gap and interrupt droplet operations. The present invention provides methods and systems for performing droplet operations on a droplet in a droplet actuator comprising maintaining substantially consistent contact between the droplet and an electrical ground while conducting multiple droplet operations on the droplet in the droplet operations gap and/or reducing the accumulation of electrical charges in the droplet operations gap during multiple droplet operations. The methods and systems reduce or eliminate bubble formation in the filler fluid of the droplet operations gap, thereby permitting completion of multiple droplet operations without interruption by bubble formation in the filler fluid in the droplet operations gap.

摘要翻译： 在液滴致动器中的液滴操作期间，在液滴操作间隙中，气泡通常在填充液中形成并且中断液滴操作。 本发明提供了用于在液滴致动器中的液滴上进行液滴操作的方法和系统，包括在液滴操作间隙内对液滴进行多个液滴操作并且/或减少 在多个液滴操作期间液滴操作间隙中的电荷。 所述方法和系统减少或消除了液滴操作间隙的填充流体中的气泡形成，从而允许完成多个液滴操作而不会由于液滴操作间隙中的填充流体中的气泡形成而中断。