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107 条记录 (耗时 0.025 秒)

    USE OF TRANS-ACTIVATION AND CIS-ACTIVATION TO MODULATE THE PERSISTENCE OF EXPRESSION OF A TRANSGENE
    45.
    发明授权
    USE OF TRANS-ACTIVATION AND CIS-ACTIVATION TO MODULATE THE PERSISTENCE OF EXPRESSION OF A TRANSGENE 有权
    反式激活和独联体活化TRANS的基因表达的持续时间调制

    公开(公告)号：EP1135515B1

    公开(公告)日：2006-05-17

    申请号：EP99964075.8

    申请日：1999-12-03

    申请人： GENVEC, INC.

    发明人： BROUGH, Douglas, E. ,  KOVESDI, Imre

    IPC分类号： C12N15/861 ,  C12N15/35 ,  C12N15/38 ,  C07K14/035 ,  C07K14/075 ,  C12N15/63

    CPC分类号： C12N15/86 ,  C07K14/005 ,  C12N15/63 ,  C12N2710/10322 ,  C12N2710/10345 ,  C12N2710/16622 ,  C12N2830/60 ,  C12N2840/60

    摘要： Provided are methods of modulating the persistence of the expression in a cell of a transgene, such as a transgene in a non-Herpes vector or in at least E4Δ adenoviral vector, and related systems. One method comprises contacting the cell with a non-Herpes vector comprising and expressing a gene encoding HSV ICP0, whereupon expression of HSV ICP0 the persistence of expression of the transgene is modulated. Further provided is a system for modulating the persistence of expression of a transgene, which system comprises a non-Herpes vector comprising (i) a gene encoding HSV ICP0 and (ii) a transgene, wherein the HSV ICP0 modulates the persistence of expression of the transgene and either the non-Herpes vector comprises the transgene or the system further comprises a vector, in which case the vector comprises the transgene. Another method comprises contacting the cell with an at least E4Δ adenoviral vector comprising (i) a transgene and (ii) a gene encoding a trans-acting factor, wherein the trans-acting factor modulates the persistence of expression of the transgene and the gene encoding the trans-acting factor is not from the E4 region of an adenovirus. Yet another method comprises contacting a cell simultaneously or sequentially with (i) an at least E4Δ adenoviral vector comprising a transgene and (ii) a viral vector comprising a gene encoding a trans-acting factor, which is not from the E4 region of an adenovirus and which modulates the persistence of expression of the transgene. Also provided is a system for modulating the persistence of expression of a transgene in an at least E4Δ adenoviral vector, which system comprises (i) an at least E4Δ adenoviral vector comprising a transgene and (ii) a gene encoding a trans-acting factor, wherein the gene encoding the trans-acting factor is not from the E4 region of an adenovirus, the trans-acting factor modulates the persistence of expression of the transgene, and either the at least E4Δ adenoviral vector comprises the gene encoding the trans-acting factor or the system comprises a viral vector, in which case the viral vector comprises the gene encoding the trans-acting factor.

    ADENOVIRAL VECTOR AND RELATED SYSTEM AND METHODS OF MAKING AND USE
    48.
    发明公开
    ADENOVIRAL VECTOR AND RELATED SYSTEM AND METHODS OF MAKING AND USE 审中-公开
    用于生产和使用腺病毒载体及相关制度和方法

    公开(公告)号：EP1425045A1

    公开(公告)日：2004-06-09

    申请号：EP02763630.7

    申请日：2002-09-13

    申请人： GENVEC, INC.

    发明人： KOVESDI, Imre

    IPC分类号： A61K48/00 ,  C12N15/10 ,  C12N15/12 ,  C12N15/64 ,  C12N15/861

    CPC分类号： C12N7/00 ,  A61K48/00 ,  C12N15/86 ,  C12N2710/10322 ,  C12N2710/10332 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2830/002 ,  C12N2830/85

    摘要： An adenoviral vector comprising an adenoviral genome comprising (i) at least one deletion in a region of the adenoviral genome selected from the group consisting of E1, E2A and E4, (ii) (a) at least one deletion in the VAI gene of the adenoviral genome, alone or in further combination with at least one deletion in the VAII gene of the adenoviral genome, (b) a recombinant VAI gene, alone or in further combination with a recombinant VAII gene, wherein the recombinant gene comprises either of a regulatable promoter in place of the native promoter or a mutated native promoter and 5' to the mutated native promoter, a pol II promoter, or (c) a dominant negative, double-stranded, RNA-dependent protein kinase (PKR) and, optionally, (iii) a polymerase II (pol II) construct comprising a pol II promoter operably linked to a coding region and/or a polymerase III (pol III) construct comprising a pol III promoter operably linked to a coding region, as well as a system comprising such an adenoviral vector and a cell line that complements the adenoviral vector, and related systems and methods.

    FLUORESCENCE DETECTION
    49.
    发明公开
    FLUORESCENCE DETECTION 审中-公开
    FLUORESZENZDETEKTION

    公开(公告)号：EP1322938A2

    公开(公告)日：2003-07-02

    申请号：EP01977436.3

    申请日：2001-10-04

    申请人： GENVEC, INC.

    发明人： CARRI N, Miguel, E. ,  MENGER, Marilyn

    IPC分类号： G01N21/64

    CPC分类号： G01N21/6486 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2710/10351

    摘要： The present invention provides methods of detecting and/or characterizing the viral vector particle content of a medium. A medium is provided and contacted with an excitation energy such that, if a viral vector particle is in the medium, an electron associated with the intrinsically fluorogenic portion of the viral vector particle will be raised to an excited energy state. The excited electron is permitted to emit radiation having an emission wavelength which is detected. The viral vector particle content of the medium then can be evaluated by comparing the detected emission wavelength with a standard signal. For example, the number of viral vector particles in a medium can be quantified by comparing the detected wavelength and its corresponding intensity to a standard signal. Similar methods for evaluating the adenoviral vector particle content of a medium and the intrinsically fluorogenic adenoviral structural protein content of a medium are provided. The invention further provides related methods for evaluating the structurally modified viral vector particle content of a medium and evaluating viral vector particle interactions in a medium.

    REPLICATION DEFICIENT ADENOVIRAL TNF VECTOR
    50.
    发明公开
    REPLICATION DEFICIENT ADENOVIRAL TNF VECTOR 有权
    REPLIKATIONSDEFIZIENTER ADENOVIRALER TNF VEKTOR

    公开(公告)号：EP1294918A2

    公开(公告)日：2003-03-26

    申请号：EP01950575.9

    申请日：2001-06-27

    申请人： GENVEC, INC.

    发明人： BROUGH, Douglas, E. ,  KING, C., Richter ,  KOVESDI, Imre

    IPC分类号： C12N15/861 ,  C12N15/28 ,  C07K14/525 ,  C12N5/10 ,  C12N7/01 ,  A61K48/00

    CPC分类号： C12N15/86 ,  A61K38/191 ,  A61K48/00 ,  C12N2710/10343 ,  C12N2810/40

    摘要： An adenoviral vector comprising (a) an adenoviral genome deficient in the E4 region of the adenoviral genome, (b) a nucleic acid sequence coding for TNF, and (c) a radiation inducible promoter operably linked to the nucleic acid sequence coding for TNF. This invention also provides an adenoviral vector comprising (a) an adenoviral genome deficient in the E4 region of the adenoviral genome, (b) a nucleic acid sequence coding for TNF, and (c) a spacer element of at least 15 base pairs in the E4 region of the adenoviral genome. A method of producing an adenoviral vector and a method of treating a tumor or cancer in a host comprising administering an anti-cancer or anti-tumor effective amount of the adenoviral vector of the present invention also is provided.