PROTEIN DETECTION METHOD
    47.
    发明公开
    PROTEIN DETECTION METHOD 审中-公开
    PROTEINNACHWEISVERFAHREN

    公开(公告)号:EP2663857A4

    公开(公告)日:2015-06-03

    申请号:EP12734237

    申请日:2012-01-11

    申请人: UNIV TORONTO

    IPC分类号: G01N27/327 G01N33/543

    摘要: Described herein are systems and methods for detecting a target analyte in a sample with electrodes, comprising a linker and an antibody attached to the linker, and measuring an electrocatalytic signal changes generated by binding of an analyte in the sample to the antibody. Also disclosed herein are kits for electrochemical detection of protein analytes.

    摘要翻译: 本文描述的是用电极检测样品中的目标分析物的系统和方法,该电极包括接头和附着于接头的抗体,以及测量通过将样品中的分析物与抗体结合而产生的电催化信号变化。 本文还公开了用于蛋白质分析物的电化学检测的试剂盒。

    BIOPROBES AND METHODS OF USE THEREOF
    49.
    发明公开
    BIOPROBES AND METHODS OF USE THEREOF 审中-公开
    VENFAHREN ZU IHRER VERWENDUNG

    公开(公告)号:EP2673381A4

    公开(公告)日:2014-10-15

    申请号:EP12744938

    申请日:2012-02-06

    申请人: UNIV TORONTO

    IPC分类号: C12Q1/68 C07H21/04 G01N33/50

    摘要: Disclosed are biomolecule based bioprobes that exhibit improved water solubility and monolayer-forming properties with substantially little or no aggregation that can appreciably interfere with binding of the bioprobes to a target nucleotide. The bioprobes may be used in conjunction with a suitable reporter system to detect very small quantities of biological markers. The bio-probes comprise a nucleobase sequence capable of hybridizing to a target nucleotide; and at least one charged functional group attached to said nucleobase sequence. Also disclosed are biosensors, and sensing devices that comprise the bio-probe. Further disclosed are suitable electrochemical reporter systems for use with bioprobes. Methods of use of these devices and probes, including for the detection of target biomarkers, including biomarkers for cancer cells or pathogens, are also included.

    摘要翻译: 公开了基于生物分子的生物探针,其表现出改善的水溶性和单层形成性质,其基本上很少或没有聚集,其可以明显地干扰生物探针与靶核苷酸的结合。 生物探针可以与合适的报道系统结合使用以检测非常少量的生物标志物。 生物探针包含能与靶核苷酸杂交的核碱基序列; 和至少一个连接到所述核碱基序列的带电官能团。 还公开了生物传感器和包括生物探针的感测装置。 还公开了适用于生物探针的电化学报告体系。 还包括使用这些装置和探针的方法,包括用于检测靶标生物标志物,包括癌细胞或病原体的生物标志物。