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449 条记录 (耗时 0.027 秒)

    AN IMPROVEMENT IN A METHOD BACTERIAL ENDOSPORE QUANTIFICATION USING LANTHANIDE DIPICOLINATE LUMINESCENCE
    62.
    发明公开
    AN IMPROVEMENT IN A METHOD BACTERIAL ENDOSPORE QUANTIFICATION USING LANTHANIDE DIPICOLINATE LUMINESCENCE 有权
    在改进定量细菌内生的方法的装置LANTHANIDDIPICOLINAT致发光

    公开(公告)号：EP1448761A4

    公开(公告)日：2005-01-05

    申请号：EP02806005

    申请日：2002-11-27

    申请人： CALIFORNIA INST OF TECHN

    发明人： PONCE ADRIAN ,  VENKATESWARAN K J

    IPC分类号： C12Q1/04 ,  C12N3/00 ,  C12Q1/00 ,  C12Q1/06 ,  G01N27/10 ,  G01N33/00

    CPC分类号： C12Q1/04

    摘要： A lanthanide is combined with a medium to be tested for endospores. The dipicolinic acid released from the endospores binds the lanthanides, which have distinctive emission (i.e., luminescence) spectra, and are detected using photoluminescence. The concentration of spores is determined by preparing a calibration curve that relates emission intensities to spore concentration for test samples with known spore concentrations. A lanthanide complex is used as the analysis reagent, and is comprised of lanthanide ions bound to multidentate ligands that increase the dipicolinic acid binding constant through a cooperative binding effect with respect to lanthanide chloride. The resulting combined effect of increasing the binding constant and eliminating coordinated water and equilibria increase the sensitivity of the endospore assay by an estimated three to four orders of magnitude over prior art of endospore detection based on lanthanide luminescence.

    DIRECTED EVOLUTION OF OXIDASE ENZYMES
    63.
    发明公开
    DIRECTED EVOLUTION OF OXIDASE ENZYMES 审中-公开
    氧化酶酶寻找EVOLUTION

    公开(公告)号：EP1290147A4

    公开(公告)日：2004-12-08

    申请号：EP00980811

    申请日：2000-11-27

    申请人： CALIFORNIA INST OF TECHN ,  ARNOLD FRANCES ,  PETROUNIA IOANNA P ,  SUN LIANHONG

    发明人： ARNOLD FRANCES H ,  PETROUNIA IOANNA P ,  SUN LIANHONG

    IPC分类号： C12N15/09 ,  C12N9/04 ,  C12N15/10 ,  C12N9/00 ,  C07H21/04 ,  C12N15/00

    CPC分类号： C12N9/0006 ,  C12N15/102

    摘要： This invention relates to the expression of improved polynucleotide and polypeptide sequences encoding for eukaryotic enzymes, particularly oxidase enzymes. The enzymes are advantageously produced in conventional or facile expression systems. Various methods for directed evolution of polynucleotide sequences can be used to obtain the improved sequences. The improved characteristics of the polypeptides or proteins generated in this manner include improved expression, enhanced activity toward one or more substrates, and increased thermal stability. In a particular embodiment, the invention relates to improved expression of the galactose oxidase gene and galactose oxidase enzymes. GAO mutants that are highly active and/or thermostable are disclosed.

    SENSORS FOR SUGARS AND OTHER METAL BINDING ANALYTES
    67.
    发明公开
    SENSORS FOR SUGARS AND OTHER METAL BINDING ANALYTES 失效
    传感器，用于糖等金属结合分析物

    公开(公告)号：EP0983511A4

    公开(公告)日：2001-12-05

    申请号：EP97908968

    申请日：1997-03-03

    申请人： CALIFORNIA INST OF TECHN

    发明人： ARNOLD FRANCES H ,  GUAN ZHIBIN ,  CHEN CHAO-TSEN ,  CHEN GUOHUA

    IPC分类号： C12Q1/00 ,  G01N33/66 ,  G01N33/68

    CPC分类号： G01N33/68 ,  C12Q1/006 ,  G01N33/66 ,  G01N2600/00 ,  G01N2650/00

    摘要： Sensors (20, 50, 70) for use in detecting the presence of sugars and other analytes (target molecules). The sensor is composed of a metal complex that binds to the target molecule and releases a proton or includes an exchangable ligand which is exchanged for the target molecule during the binding interaction between the metal complex and the target molecule. The result of the binding interaction is the release of a proton, hydroxide ion or ligand species generated during the ligand exchange. Measurement of the release of proton, hydroxide ion or other ligand species from the sensor (20, 50, 70) provides an indirect indication of target molecule concentration. The metal complexes may be attached to support structures (10, 12) to provide both anchoring and positioning of the metal ions to increase selectivity of sugar/metal complex interactions. Detection systems in which pH is used as an indication of proton or hydroxide release are disclosed, as are detection systems in which Cl release is used. Methods for monitoring the concentrations of sugars and related molecules using the metal based sensors (20, 50, 70) are also disclosed.

    CMOS INTEGRATION SENSOR WITH FULLY DIFFERENTIAL COLUMN READOUT CIRCUIT FOR LIGHT ADAPTIVE IMAGING
    68.
    发明公开
    CMOS INTEGRATION SENSOR WITH FULLY DIFFERENTIAL COLUMN READOUT CIRCUIT FOR LIGHT ADAPTIVE IMAGING 审中-公开
    CMOS-INTEGRATIONSSENSOR MIT VOLL DIFFERENTIELLER SPALTENAUSLESESCHALTUNG ZUMHELLICHKEITSADAPTIVEN ABBILDEN

    公开(公告)号：EP1086578A4

    公开(公告)日：2001-09-26

    申请号：EP99912623

    申请日：1999-03-16

    申请人： CALIFORNIA INST OF TECHN

    发明人： PAIN BEDABRATA ,  ZHOU ZHIMIN ,  FOSSUM ERIC R

    IPC分类号： H04N5/347 ,  H04N5/357 ,  H04N5/374 ,  H04N5/378 ,  H04N3/14 ,  H04N3/15

    CPC分类号： H04N5/374 ,  H04N5/347 ,  H04N5/3575 ,  H04N5/378

    摘要： An imager (10) that is better suited for low-light detection capability. In accordance with a preferred embodiment, the imager may be easily configured to provide an imager (10) having multi-resolution capability where SNR can be adjusted for optimum low-level detectibility. Multi-resolution signal processing functionality is provided on-chip to achieve high speed imaging, employs an improved pixel binning approach with fully differential circuits situated so that all extraneous and pick-up noise is eliminated. The current implementation requires no frame transfer memory, thereby reducing chip size. The reduction in area enables larger area format light adaptive imager implementations.

    摘要翻译： 成像器（10）更适合低光检测能力。 根据优选实施例，成像器可以容易地配置为提供具有多分辨率能力的成像器（10），其中可以调节SNR以实现最佳的低水平可检测性。 片上提供多分辨率信号处理功能以实现高速成像，采用改进的像素合并方法，配置全差分电路，以消除所有无关和拾音噪声。 目前的实现不需要帧转移存储器，从而减小了芯片尺寸。 面积的减小使得更大面积的光自适应成像器实现成为可能。

    METHOD AND COMPOSITIONS FOR CONTROLLING GENE EXPRESSION
    69.
    发明公开
    METHOD AND COMPOSITIONS FOR CONTROLLING GENE EXPRESSION 失效
    方法和组合物控制基因表达的

    公开(公告)号：EP1007726A4

    公开(公告)日：2001-04-25

    申请号：EP97931076

    申请日：1997-06-06

    申请人： CALIFORNIA INST OF TECHN

    发明人： DAVIDSON ERIC H

    IPC分类号： C07K16/00 ,  C07K16/46 ,  C12N15/12 ,  C12Q1/68 ,  C07H21/04 ,  C12N15/13 ,  C12P21/08

    CPC分类号： C07K16/00

    摘要： The present invention is directed to methods and compositions useful for altering the transcriptional expression of genes in eukaryotic cells. The invention employs novel antibody derivative molecules which function to recognize and bind to specific cis-regulatory DNA sequence elements of a eukaryotic gene. When two antibody derivative molecules are bound to adjacent cis-regulatory DNA sequence elements of a gene, those molecules may interact to form an antibody binding site which is capable of recognizing and binding to a transcription factor protein for the target gene, thereby affecting the functionality of that transcription factor protein and, in turn, the transcriptional activity of the gene. Also provided herein are isolated nucleic acids encoding the novel antibody derivative molecules of the present invention and expression vectors comprising those nucleic acids.