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    PRODUCTION OF GLYCOPROTEINS WITH MAMMALIAN-LIKE N-GLYCANS IN FILAMENTOUS FUNGI
    64.
    发明公开
    PRODUCTION OF GLYCOPROTEINS WITH MAMMALIAN-LIKE N-GLYCANS IN FILAMENTOUS FUNGI 审中-公开
    哺乳动物丝状真菌中哺乳动物样N-糖苷生产糖蛋白

    公开(公告)号：EP3172333A1

    公开(公告)日：2017-05-31

    申请号：EP15738703.6

    申请日：2015-07-21

    申请人： Glykos Finland Oy

    发明人： NATUNEN, Jari ,  LEPPÄNEN, Anne ,  SALMINEN, Heidi ,  HILTUNEN, Jukka ,  KANERVA, Anne ,  HEISKANEN, Annamari ,  WESTERHOLM-PARVINEN, Ann ,  SCHMIDT, Georg ,  HUUSKONEN, Anne ,  MUSTALAHTI, Eero ,  LANDOWSKI, Christopher ,  SALOHEIMO, Markku ,  SAARINEN, Juhani ,  SOMMER, Benjamin Patrick ,  WAHL, Ramon ,  OSTERMEIER, Christian ,  HELK, Bernhard

    IPC分类号： C12P21/00 ,  C12N9/10 ,  C12N9/24

    摘要： The present disclosure relates to compositions and methods useful for the production of recombinant glycoproteins in filamentous fungal cells, such as Trichoderma cells, wherein at least 90% (mol %), preferably at least 95% of the total neutral N-glycans of said produced recombinant glycoprotein are mammalian-like N-glycans. More specifically, the invention provides a filamentous fungal cell comprising i. one or more mutations that reduces or eliminates one or more endogenous protease activity compared to a parental filamentous fungal cell which does not have said mutation(s); ii.a polynucleotide encoding a heterologous catalytic subunit of oligosaccharyl transferase; iii. a recombinant polynucleotide for increasing α1, 2 mannosidase activity;and, iv. a recombinant polynucleotide encoding said heterologous glycoprotein.

    摘要翻译： 本公开涉及用于在丝状真菌细胞例如木霉属细胞中产生重组糖蛋白的组合物和方法，其中所述产生的全部中性N-聚糖的至少90％（mol％），优选至少95％ 重组糖蛋白是哺乳动物样N-聚糖。 更具体地说，本发明提供了一种丝状真菌细胞，其包含i。 与不具有所述突变的亲本丝状真菌细胞相比，减少或消除一种或多种内源性蛋白酶活性的一种或多种突变; ii。编码寡糖基转移酶的异源催化亚基的多核苷酸; III。 用于增加α1,2甘露糖苷酶活性的重组多核苷酸;和iv。 编码所述异源糖蛋白的重组多核苷酸。

    USE OF A PROTEOLYTIC ENZYME
    67.
    发明公开
    USE OF A PROTEOLYTIC ENZYME 审中-公开
    使用蛋白水解酶

    公开(公告)号：EP2764092A1

    公开(公告)日：2014-08-13

    申请号：EP12837914.6

    申请日：2012-10-04

    申请人： Glykos Finland Oy

    发明人： KERKELÄ, Erja ,  HAKKARAINEN, Tanja ,  NYSTEDT, Johanna ,  PARTANEN, Jukka

    IPC分类号： C12N5/0789

    CPC分类号： A61K35/28 ,  C12N5/0006 ,  C12N5/0647 ,  C12N2501/734

    摘要： The present invention relates to a hematopoietic stem cell and/or a population thereof having a specific profile of cell surface proteins and/or proteoglycans. The present invention also relates to use of proteolytic enzymes, such as pronase and pronase-like enzymes in the modification of the cell surface of a hematopoietic stem cell. The present invention further relates to a method of modifying the cell surface of a hematopoietic stem cell by treatment with proteolytic enzymes, such as pronase and pronase-like enzymes.