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    METHODS AND COMPOSITIONS FOR EFFECTING HOMOLOGOUS RECOMBINATION
    61.
    发明公开
    METHODS AND COMPOSITIONS FOR EFFECTING HOMOLOGOUS RECOMBINATION 审中-公开
    方法和组合物的有效同源重组

    公开(公告)号：EP1278832A1

    公开(公告)日：2003-01-29

    申请号：EP01925779.9

    申请日：2001-05-03

    申请人： Institut Curie ,  CENTRE NATIONAL DELA RECHERCHE SCIENTIFIQUE (CNRS) ,  MUSEUM NATIONAL D'HISTOIRE NATURELLE ,  INSTITUT NATIONAL DE LA SANTE ETDE LA RECHERCHE MEDICALE (INSERM)

    发明人： DUTREIX, Marie ,  SUN, Jian-Sheng ,  BIET, Elodie ,  MAURISSE, Rosalie ,  FEUGEAS Jean Paul

    IPC分类号： C12N15/10 ,  C12N15/90 ,  A61K48/00

    CPC分类号： C12N15/102 ,  C12N15/902

    摘要： This invention concerns a method for effecting homologous recombination between a native nucleic acid segment in a cell and a donor nucleic acid segment introduced into the cell, which comprises: a) introducing into a cell a nucleic acid delivery system comprising: (i) an oligonucleotide third strand which comprises a base sequence capable of forming a triple helix at a binding region on one or both strands of a native nucleic acid segment, (ii) a donor nucleic acid, comprising a nucleic acid sequence sufficiently homologous to the native nucleic acid segment such that the donor sequence is capable of undergoing homologous recombination with the native sequence at the target region, (iii) an adapter segment comprising an oligonucleotide sequence able to bind at least a portion of said donor nucleic acid through Watson-Crick base pairing, the adapter segment being linked to said oligonucleotide third strand, b) allowing the oligonucleotide to bind to the native nucleic acid segment to form a triple helix nucleic acid, thereby inducing homologous recombination at the native nucleic acid segment target region; and c) allowing homologous recombination to occur between the native and donor nucleic acid segments. The method may namely be implemented for effecting gene alteration or mutation repair at a specific sequence site on a native DNA.

    DNA MANIPULATION METHODS , APPLICATIONS FOR SYNTHETIC ENZYMES AND USE FOR POLYKETIDE PRODUCTION
    64.
    发明公开
    DNA MANIPULATION METHODS , APPLICATIONS FOR SYNTHETIC ENZYMES AND USE FOR POLYKETIDE PRODUCTION 审中-公开
    DNS操作方法，用于人工酶和方法的应用程序用于生产聚酮化合物

    公开(公告)号：EP1190045A2

    公开(公告)日：2002-03-27

    申请号：EP00940533.3

    申请日：2000-06-12

    申请人： Qxyz Limited

    发明人： RANGANATHAN, Anand,ICGEB

    IPC分类号： C12N15/10 ,  C12N15/66 ,  C12N15/52 ,  C12N15/90 ,  C12P17/06 ,  C12P17/08

    CPC分类号： C12N15/1093 ,  C12N15/10 ,  C12N15/52 ,  C12N15/64 ,  C12N15/66 ,  C12N15/902 ,  C12P17/06 ,  C12P17/08

    摘要： The invention comprises a method of assembling several DNA units in sequence in a DNA construct and all derivatives of this method. In particular the production of synthetic enzymes is contemplated. Each DNA unit is provided with the same restriction enzyme recognition site at its 5' and 3' ends. The restriction recognition site at its 3' end being combined with a recognition site for a DNA modification enzyme. A DNA construct having the same or a compatible accessible restriction site, as provided in the DNA unit, is cleaved at the restriction site by the appropriate restriction enzyme. The desired DNA unit is then inserted into the DNA construct, this ligated product subsequently being brought into contact with a DNA modification enzyme such that the restriction site at the 3' end of the inserted DNA unit is abolished. The ligated product is then cleaved at the remaining unmodified restriction recognition site and a subsequent DNA unit is inserted. This process is repeated introducing each desired DNA unit to give a DNA construct containing all the desired units in sequence.

    Methods and compositions for effecting homologous recombination
    65.
    发明公开
    Methods and compositions for effecting homologous recombination 审中-公开
    Methoden und Zusammensetzungen zur effizienten homologen Rekombination

    公开(公告)号：EP1152058A1

    公开(公告)日：2001-11-07

    申请号：EP00401218.3

    申请日：2000-05-03

    申请人： Institut Curie ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS) ,  MUSEUM NATIONAL D'HISTOIRE NATURELLE ,  INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM)

    发明人： Dutreix, Marie ,  Sun, Jian-Sheng ,  Biet, Elodie ,  Maurisse, Rosalie ,  Feugeas, Jean Paul

    IPC分类号： C12N15/10 ,  C12N15/90

    CPC分类号： C12N15/102 ,  C12N15/902

    摘要： This invention concerns a method for effecting homologous recombination between a native nucleic acid segment in a cell and a donor nucleic acid segment introduced into the cell, which comprises :

    a) introducing into a cell a nucleic acid delivery system comprising:

    (i) an oligonucleotide third strand which comprises a base sequence capable of forming a triple helix at a binding region on one or both strands of a native nucleic acid segment,
    (ii) a donor nucleic acid, comprising a nucleic acid sequence sufficiently homologous to the native nucleic acid segment such that the donor sequence is capable of undergoing homologous recombination with the native sequence at the target region,
    (iii) an adapter segment comprising an oligonucleotide sequence able to bind at least a portion of said donor nucleic acid through Watson-Crick base pairing, the adapter segment being linked to said oligonucleotide third strand,

    b) allowing the oligonucleotide to bind to the native nucleic acid segment to form a triple helix nucleic acid, thereby inducing homologous recombination at the native nucleic acid segment target region ; and
    c) allowing homologous recombination to occur between the native and donor nucleic acid segments.

    The method may namely be implemented for effecting gene alteration or mutation repair at a specific sequence site on a native DNA.

    摘要翻译： 本发明涉及一种用于在细胞中引入天然核酸片段和引入细胞的供体核酸片段之间进行同源重组的方法，其包括：a）向细胞导入核酸递送系统，其包含：（i）寡核苷酸 第三链，其包含能够在天然核酸片段的一条或两条链上的结合区上形成三螺旋的碱基序列，（ii）供体核酸，其包含与天然核酸片段足够同源的核酸序列 使得供体序列能够在靶区域与天然序列进行同源重组，（iii）衔接子部分，其包含能够通过Watson-Crick碱基配对结合所述供体核酸的至少一部分的寡核苷酸序列， 衔接子链与所述寡核苷酸第三链连接，b）使寡核苷酸与天然核酸片段结合 形成三重螺旋核酸，从而在天然核酸区段靶区域诱导同源重组; 和c）允许在天然和供体核酸片段之间发生同源重组。 该方法可以被实施用于在天然DNA上的特定序列位点处进行基因改变或突变修复。

    Production of proteins using homologous recombination
    66.
    发明公开
    Production of proteins using homologous recombination 无效
    Herstellung von Proteinen手套同源人Rekombination

    公开(公告)号：EP0747485A1

    公开(公告)日：1996-12-11

    申请号：EP95119039.6

    申请日：1990-11-06

    申请人： CELL GENESYS, INC.

    发明人： Skoultchi, Arthur I.

    IPC分类号： C12N15/85 ,  C12N5/10 ,  C07K14/71 ,  C12N15/90

    CPC分类号： C12N15/81 ,  C07K14/505 ,  C07K14/535 ,  C07K14/59 ,  C12N15/85 ,  C12N15/86 ,  C12N15/902 ,  C12N2795/00011 ,  C12N2800/206 ,  C12N2840/20 ,  C12N2840/44

    摘要： Methods and compositions are provided for expression of mammalian genes in culture. An amplifiable gene is introduced by homologous recombination in juxtaposition to a target gene, the resulting combination of amplifiable gene and target gene transferred to a convenient host and the target gene amplified by means of the amplifiable gene. The resulting expression host may then be grown in culture with enhanced expression of the target gene.

    摘要翻译： 提供了用于在培养物中表达哺乳动物基因的方法和组合物。 通过与靶基因并置引入同源重组引入可扩增基因，将可扩增基因和靶基因的组合转移到方便的宿主，并通过可扩增基因扩增靶基因。 然后可以使得到的表达宿主在靶基因增强表达的培养物中生长。

    METHOD OF INTEGRATING GENE INTO CHROMOSOME OF $i(LACTOBACILLUS DELBRUECKII) SPECIES AND PRODUCT OF GENE INTEGRATION
    67.
    发明公开
    METHOD OF INTEGRATING GENE INTO CHROMOSOME OF $i(LACTOBACILLUS DELBRUECKII) SPECIES AND PRODUCT OF GENE INTEGRATION 失效
    METHOD FOR整合基因到染色体甲-i（LACTOKACILLUS德氏）种及产品，遗传融合。

    公开(公告)号：EP0603416A1

    公开(公告)日：1994-06-29

    申请号：EP93914972.0

    申请日：1993-07-08

    申请人： MEIJI MILK PRODUCTS COMPANY LIMITED

    发明人： SASAKI, Takashi ,  SASAKI, Yasuko ,  ITO, Yoshiyuki ,  OTSU, Kumi

    IPC分类号： C12N15/90 ,  C12N1/21

    CPC分类号： C12N9/0006 ,  C12N15/746 ,  C12N15/902

    摘要： A product of gene integration prepared by integrating an arbitrary gene into a chromosomal DNA of a bacillus of Lactobacillus delbrueckii species through a method which comprises constructing an integration plasmid by combining pAMβ1 plasmid with a target gene, subjecting the obtained plasmid to conjugation transfer and homologous recombination to thereby integrate only the target gene into the chromosomal DNA, and eliminating the DNA sequence of the pAMβ1 as a vector. The product can be widely applied to the production of food, drug and feed.

    摘要翻译： 通过INTEGRA婷准备任意的基因导入通过方法德氏乳杆菌属物种的芽孢杆菌的染色体DNA的基因整合的一种产品，它通过组合PAM的β1个质粒与靶基因，对所得质粒接合转移，并且包括整合质粒的构建 同源重组，从而整合只有目标基因到染色体DNA中，和消除的βPAM 1作为载体的DNA序列。 该产品可广泛应用于生产食品，药品和饲料。

    RECOMBINANT CELLS THAT HIGHLY EXPRESS CHROMOSOMALLY-INTEGRATED HETEROLOGOUS GENES
    69.
    发明公开
    RECOMBINANT CELLS THAT HIGHLY EXPRESS CHROMOSOMALLY-INTEGRATED HETEROLOGOUS GENES 无效
    重组细胞，强有力的染色体INTEGRATED外源基因。

    公开(公告)号：EP0560885A1

    公开(公告)日：1993-09-22

    申请号：EP92901454.0

    申请日：1991-12-04

    申请人： UNIVERSITY OF FLORIDA

    发明人： INGRAM, Lonnie, O. ,  OHTA, Kazuyoshi

    IPC分类号： C12N15 ,  C12N1 ,  C12N9 ,  C12P7 ,  C12R1

    CPC分类号： C12N9/0006 ,  C12N9/88 ,  C12N15/01 ,  C12N15/70 ,  C12N15/74 ,  C12N15/902 ,  C12P7/065 ,  Y02E50/17

    摘要： Selon l'invention, l'on obtient des cellules hôtes recombinées qui comprennent (A) un segment polynucléotidique hétérologue codant pour des polypeptides, intégré de façon stable dans un chromosome, qui est sous le contrôle transcriptionnel d'un promoteur endogène, et (B) une mutation qui effectue l'expression augmentée du segment hétérologue, cela ayant pour résultat une production, par les cellules hôtes de chaque polypeptide codé par ce segment, plus élevée, si l'on compare avec la production de chaque polypeptide, par les cellules hôtes, en l'absence de la mutation. L'expression augmentée ainsi obtenue est retenue en l'absence des conditions favorables aux cellules qui font preuve d'une telle expression augmentée. Lorsque le segment intégré comprend, par exemple, des gènes de la production d'éthanol provenant d'un producteur d'éthanol efficace tel que le Zymomonas mobilis, l'Escherichia coli recombiné et d'autres cellules bactériennes entériques, selon l'invention, sont capables de transformer en éthanol, de façon efficace, une large gamme de sucres dérivés de la biomasse.