公开(公告)号：EP1836319A2

公开(公告)日：2007-09-26

申请号：EP06717401.1

申请日：2006-01-06

申请人： Applera Corporation

发明人： MARTIN, Patrick K. ,  SIMPSON, David A. ,  HARDY, Christine D.

IPC分类号： C12Q1/68 ,  C12N15/62

CPC分类号： C12P19/34 ,  C07K14/195 ,  C07K2319/71 ,  C07K2319/80 ,  C12N9/1252 ,  C12N9/96 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/6846 ,  C12Q2522/101 ,  C12Q2521/101 ,  C12Q2563/119 ,  C12Q2527/101

摘要： Polypeptides having nucleic acid binding activity are provided. Methods of using polypeptides having nucleic acid binding activity are provided. Fusion proteins and methods of using fusion proteins are provided. Fusion proteins comprising a polymerase and a nucleic acid binding polypeptide are provided. Fusion proteins comprising a reverse transcriptase and a nucleic acid binding polypeptide are provided. Methods are provided for amplifying a nucleic acid sequence using a fusion protein comprising a nucleic acid binding polypeptide and a polymerase. Methods are provided for amplifying a nucleic acid sequence using a fusion protein comprising a nucleic acid binding polypeptide and a reverse transcriptase.

公开(公告)号：EP1483415A4

公开(公告)日：2006-02-01

申请号：EP03742781

申请日：2003-02-19

申请人： UNIV VIRGINIA ,  CHROMA THERAPEUTICS LTD

发明人： ALLIS C DAVID ,  BRUNS DAVID E ,  DRUMMOND ALAN H

IPC分类号： C07K1/22 ,  C07K16/18 ,  C12M1/00 ,  C12N15/09 ,  C12P19/34 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/68 ,  G01N37/00

CPC分类号： G01N33/6842 ,  C12Q1/6809 ,  G01N33/5308 ,  G01N33/68 ,  G01N33/6875 ,  C12Q2565/113 ,  C12Q2563/119

摘要： The present invention relates to the use of antibodies directed against specific histone amino terminus modifications as diagnostic indicators of disease or congenital defects. In one embodiment, nucleosomes are isolated from a blood or serum sample of a patient using histone specific antibodies and the accompanying DNA is purified and analyzed for diagnostic and screening purposes.

公开(公告)号：EP1559790A1

公开(公告)日：2005-08-03

申请号：EP04002214.7

申请日：2004-02-02

申请人： INTERNATIONAL UNIVERSITY BREMEN GMBH ,  Universität Basel

发明人： Schwaneberg, Ulrich ,  Winterhalter, Mathias ,  Meier, Wolfgang

IPC分类号： C12Q1/68 ,  G01N33/58

CPC分类号： C12N15/1006 ,  C12Q2563/119 ,  C12Q2563/161

摘要： Die Erfindung betrifft Verfahren zum Abtrennen von Substanzen aus flüssigen Medien und dazu geeignete Trennmittel.

摘要翻译： 在泡囊（A）中，为了粘合在膜中具有含有两亲分子（AM）和成孔单元（PFU）的膜的物质（I），以提供进入（A）的内部的新特征 内部含有（I）的结合物质（BS），并且BS基本上不能通过由PFU形成的孔扩散。 还包括用于通过使用（A）的结合和任选地随后释放核酸（NA）的方法的独立权利要求。

公开(公告)号：EP1231282A3

公开(公告)日：2005-05-18

申请号：EP01204634.8

申请日：1991-12-06

申请人： Affymetrix, Inc.

发明人： Fodor, Stephen P. A. ,  Solas, Dennis W. ,  Dower, William J.

IPC分类号： C12Q1/68 ,  G01N33/566 ,  G01N33/48 ,  C07H15/12

CPC分类号： G03F7/00 ,  B01J19/0046 ,  B01J2219/00315 ,  B01J2219/00432 ,  B01J2219/00434 ,  B01J2219/00436 ,  B01J2219/00459 ,  B01J2219/00468 ,  B01J2219/00475 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00531 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00725 ,  B82Y10/00 ,  B82Y30/00 ,  C07B2200/11 ,  C07H19/10 ,  C07H21/00 ,  C07K1/042 ,  C07K1/045 ,  C07K17/06 ,  C07K17/14 ,  C12Q1/6804 ,  C12Q1/6809 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q1/6874 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N15/1475 ,  G03F7/265 ,  G03F7/38 ,  G11C13/0014 ,  G11C13/0019 ,  C12Q2565/513 ,  C12Q2565/507 ,  C12Q2545/101 ,  C12Q2525/185 ,  C12Q2565/514 ,  C12Q2563/173 ,  C12Q2563/119 ,  C12Q2563/149 ,  C12Q2525/151 ,  C12Q2525/179 ,  C12Q2525/204

摘要： Biological polymers such as polynucleotides are identified as targets in a sample by exposing the sample to a collection of substrate-bound probes. The substrates are beads and each bead has a different probe. When a target is present in a sample it is bound by one or more of the probes, and thereby bound to certain beads in the collection. Beads binding targets are separated from beads failing to bind targets, e.g. by a cell sorter, and then subjected to further analysis. Beads in the collection are additionally tagged (i.e. coded) in such a way that the identity of any probe, and thus the bound target, is revealed when the identity of the tag is determined.

公开(公告)号：EP0834576B1

公开(公告)日：2002-01-16

申请号：EP97116548.5

申请日：1991-12-06

申请人： Affymetrix, Inc. (a Delaware Corporation)

发明人： Fodor, Stephen P.A. ,  Dower, William J. ,  Solas, Dennis W.

IPC分类号： C12Q1/68 ,  G01N33/566 ,  G01N33/48 ,  C07H15/12

CPC分类号： G03F7/00 ,  B01J19/0046 ,  B01J2219/00315 ,  B01J2219/00432 ,  B01J2219/00434 ,  B01J2219/00436 ,  B01J2219/00459 ,  B01J2219/00468 ,  B01J2219/00475 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00531 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00725 ,  B82Y10/00 ,  B82Y30/00 ,  C07B2200/11 ,  C07H19/10 ,  C07H21/00 ,  C07K1/042 ,  C07K1/045 ,  C07K17/06 ,  C07K17/14 ,  C12Q1/6804 ,  C12Q1/6809 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q1/6874 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N15/1475 ,  G03F7/265 ,  G03F7/38 ,  G11C13/0014 ,  G11C13/0019 ,  C12Q2565/513 ,  C12Q2565/507 ,  C12Q2545/101 ,  C12Q2525/185 ,  C12Q2565/514 ,  C12Q2563/173 ,  C12Q2563/119 ,  C12Q2563/149 ,  C12Q2525/151 ,  C12Q2525/179 ,  C12Q2525/204

公开(公告)号：EP0834575B1

公开(公告)日：2001-11-28

申请号：EP97116541.0

申请日：1991-12-06

申请人： Affymetrix, Inc. (a Delaware Corporation)

发明人： Fodor, Stephen P. A. ,  Dower, William J. ,  Solas, Dennis W.

IPC分类号： C12Q1/68 ,  G01N33/566 ,  G01N33/48 ,  C07H15/12

CPC分类号： G03F7/00 ,  B01J19/0046 ,  B01J2219/00315 ,  B01J2219/00432 ,  B01J2219/00434 ,  B01J2219/00436 ,  B01J2219/00459 ,  B01J2219/00468 ,  B01J2219/00475 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00531 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00725 ,  B82Y10/00 ,  B82Y30/00 ,  C07B2200/11 ,  C07H19/10 ,  C07H21/00 ,  C07K1/042 ,  C07K1/045 ,  C07K17/06 ,  C07K17/14 ,  C12Q1/6804 ,  C12Q1/6809 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q1/6874 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N15/1475 ,  G03F7/265 ,  G03F7/38 ,  G11C13/0014 ,  G11C13/0019 ,  C12Q2565/513 ,  C12Q2565/507 ,  C12Q2545/101 ,  C12Q2525/185 ,  C12Q2565/514 ,  C12Q2563/173 ,  C12Q2563/119 ,  C12Q2563/149 ,  C12Q2525/151 ,  C12Q2525/179 ,  C12Q2525/204

公开(公告)号：EP0834575A3

公开(公告)日：1999-10-20

申请号：EP97116541.0

申请日：1991-12-06

申请人： AFFYMAX TECHNOLOGIES N.V.

发明人： Fodor, Stephen P. A. ,  Dower, William J. ,  Solas, Dennis W.

IPC分类号： C12Q1/68 ,  C07H21/00

CPC分类号： G03F7/00 ,  B01J19/0046 ,  B01J2219/00315 ,  B01J2219/00432 ,  B01J2219/00434 ,  B01J2219/00436 ,  B01J2219/00459 ,  B01J2219/00468 ,  B01J2219/00475 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00531 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00725 ,  B82Y10/00 ,  B82Y30/00 ,  C07B2200/11 ,  C07H19/10 ,  C07H21/00 ,  C07K1/042 ,  C07K1/045 ,  C07K17/06 ,  C07K17/14 ,  C12Q1/6804 ,  C12Q1/6809 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q1/6874 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N15/1475 ,  G03F7/265 ,  G03F7/38 ,  G11C13/0014 ,  G11C13/0019 ,  C12Q2565/513 ,  C12Q2565/507 ,  C12Q2545/101 ,  C12Q2525/185 ,  C12Q2565/514 ,  C12Q2563/173 ,  C12Q2563/119 ,  C12Q2563/149 ,  C12Q2525/151 ,  C12Q2525/179 ,  C12Q2525/204

摘要： The present invention provides methods and apparatus for sequencing, fingerprinting and mapping biological polymers, particularly polynucleotides. The methods make use of a plurality of positionally distinct sequence specific recognition reagents, such as polynucleotides. The apparatus employs a substrate comprising positionally distinct sequence specific recognition reagents, such as polynucleotides, which are preferably localized at high densities. The methods and apparatus of the present invention can be used for determining the sequence of polynucleotides, mapping polynucleotides, and developing polynucleotide fingerprints. Polynucleotide fingerprints can be used for identifying individuals, tissue samples, pathological conditions, genetic diseases, infectious diseases, and other applications. Polynucleotide fingerprints can also be used for classification of biological samples, including taxonomy, and to characterize their sources. The invention also provides polynucleotide mapping, fingerprinting, and sequencing as valuable laboratory research tools for use in biological investigations.

公开(公告)号：EP0834576A3

公开(公告)日：1999-06-16

申请号：EP97116548.5

申请日：1991-12-06

申请人： AFFYMAX TECHNOLOGIES N.V.

发明人： Fodor, Stephen P.A. ,  Dower, William J. ,  Solas, Dennis W.

IPC分类号： C12Q1/68 ,  C07H21/00

CPC分类号： G03F7/00 ,  B01J19/0046 ,  B01J2219/00315 ,  B01J2219/00432 ,  B01J2219/00434 ,  B01J2219/00436 ,  B01J2219/00459 ,  B01J2219/00468 ,  B01J2219/00475 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00531 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00725 ,  B82Y10/00 ,  B82Y30/00 ,  C07B2200/11 ,  C07H19/10 ,  C07H21/00 ,  C07K1/042 ,  C07K1/045 ,  C07K17/06 ,  C07K17/14 ,  C12Q1/6804 ,  C12Q1/6809 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q1/6874 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N15/1475 ,  G03F7/265 ,  G03F7/38 ,  G11C13/0014 ,  G11C13/0019 ,  C12Q2565/513 ,  C12Q2565/507 ,  C12Q2545/101 ,  C12Q2525/185 ,  C12Q2565/514 ,  C12Q2563/173 ,  C12Q2563/119 ,  C12Q2563/149 ,  C12Q2525/151 ,  C12Q2525/179 ,  C12Q2525/204

摘要： The present invention provides methods and apparatus for sequencing, fingerprinting and mapping biological polymers, particularly polynucleotides. The methods make use of a plurality of positionally distinct sequence specific recognition reagents, such as polynucleotides. The apparatus employs a substrate comprising positionally distinct sequence specific recognition reagents, such as polynucleotides, which are preferably localized at high densities. The methods and apparatus of the present invention can be used for determining the sequence of polynucleotides, mapping polynucleotides, and developing polynucleotide fingerprints. Polynucleotide fingerprints can be used for identifying individuals, tissue samples, pathological conditions, genetic diseases, infectious diseases, and other applications. Polynucleotide fingerprints can also be used for classification of biological samples, including taxonomy, and to characterize their sources. The invention also provides polynucleotide mapping, fingerprinting, and sequencing as valuable laboratory research tools for use in biological investigations.

公开(公告)号：EP0635069B1

公开(公告)日：1997-10-29

申请号：EP93912131.5

申请日：1993-04-02

申请人： THE PERKIN-ELMER CORPORATION

发明人： GROSSMAN, Paul, David ,  FUNG, Steven ,  MENCHEN, Steven, Michael ,  WOO, Sam, Lee ,  WINN-DEEN, Emily, Susan

IPC分类号： C12Q1/68 ,  B01D57/02

CPC分类号： C12Q1/6869 ,  C12Q1/6816 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  Y10T436/143333 ,  C12Q2565/125 ,  C12Q2525/203 ,  C12Q2525/197 ,  C12Q2563/167 ,  C12Q2535/101 ,  C12Q2565/629 ,  C12Q2563/155 ,  C12Q2563/107 ,  C12Q2531/137 ,  C12Q2563/119 ,  C12Q2549/119 ,  C12Q2525/161 ,  C12Q2521/501 ,  C12Q2563/131 ,  C12Q2525/101 ,  C12Q2531/113

摘要： Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In practicing the method, a plurality of different-sequence probe pairs are added to a target polynucleotide, where each probe pair includes two polynucleotide probe elements which are complementary in sequence to adjacent portions of a selected one of the target sequences in the target polynucleotide. In each probe pair, one of the probe elements contains a non-polynucleotide polymer chain which imparts a distinctive elctrophoretic mobility in a sieving matrix, to the associated probe pair, when the elements in the pair are ligated. The other element in the pair contains a detectable reporter label. After the probe pairs have been allowed to hybridize with the target polynucleotide, the hybridized polynucleotides are treated under conditions effective to ligate the end subunits of target-bound probe elements when their end subunits are base-paired with adjacent target bases. The ligated probe pairs are then released from the target polynucleotide and separated electrophoretically in a sieving matrix, or chromatographically.