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    FUMONISIN DETOXIFICATION COMPOSITIONS AND METHODS
    75.
    发明公开
    FUMONISIN DETOXIFICATION COMPOSITIONS AND METHODS 失效
    Fumonisin脱毒组合物和方法

    公开(公告)号：EP0988383A1

    公开(公告)日：2000-03-29

    申请号：EP98933188.9

    申请日：1998-07-07

    申请人： PIONEER HI-BRED INTERNATIONAL, INC.

    发明人： DUVICK, Jonathan ,  MADDOX, Joyce, R. ,  ROOD, Tracy, A. ,  WANG, Xun ,  BOWEN, Benjamin, A. ,  GILLIAM, Jacob, T.

    IPC分类号： C12N15/55 ,  C12N15/82 ,  C12N15/65 ,  C12Q1/44 ,  C12Q1/68

    CPC分类号： A01N63/00 ,  C12N9/00 ,  C12N9/16 ,  C12N9/18 ,  C12N15/8209 ,  C12N15/8242 ,  C12N15/8282

    摘要： Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin and/or capable of growing on fumonisin as a sole carbon source. Using this method, several organisms have been identified. These organisms can be used to isolate the enzymes and the genes responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for fumonisin degrading enzymes can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infections. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.

    摘要翻译： 用于鉴定能够降解伏马菌素的生物体的方法。 可以将伏马菌素掺入到培养基中以选择对伏马菌素有抗性和/或能够作为唯一碳源在伏马菌素上生长的生物体。 使用这种方法，已经确定了几种生物。 这些生物体可用于分离酶和负责赋予伏马菌素抗性的基因。 该基因可被克隆并插入合适的表达载体中，以便可进一步表征蛋白质。 此外，编码伏马菌素降解酶的DNA可用于转化通常对镰刀菌或其他产生毒素的真菌感染敏感的植物细胞。 植物可以从转化的植物细胞中再生。 通过这种方式，可以生产具有降解伏马菌素能力的转基因植物以及生产降解酶的能力。 还公开了谷物，谷物加工，青贮饲料，粮食作物以及动物饲料和瘤胃微生物的解毒方法。

    SINGLE-STEP EXCISION MEANS
    79.
    发明公开
    SINGLE-STEP EXCISION MEANS 失效
    一步法切出

    公开(公告)号：EP0922097A1

    公开(公告)日：1999-06-16

    申请号：EP97913984.0

    申请日：1997-03-27

    申请人： COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION ,  THE AUSTRALIAN NATIONAL UNIVERSITY

    发明人： SURIN, Brian, Peter ,  DE FEYTER, Robert, Charles ,  GRAHAM, Michael, Wayne ,  WATERHOUSE, Peter, Michael ,  KEESE, Paul, Konrad ,  ALI, Shahjahan

    IPC分类号： C12N15 ,  A01H5 ,  C12N5

    CPC分类号： C12N15/8217 ,  C12N15/8209 ,  C12N15/8213 ,  C12N15/8242 ,  C12N15/8265

    摘要： The present invention is directed to the genetic transformation using multiple genetic sequences, wherein one of said genetic sequences encodes a polypeptide possessing excision activity, specifically a site-specific recombinase activity linked to a transgene unit and the use of this genetic construct in the removal of transgenes therefrom. The present invention provides the means to produce genetically-transformed organisms, in particular plants, in which selectable marker genes have been removed, thereby facilitating multiple sequential genetic transformation events using the same selectable marker gene. Accordingly, the invention provides the means for regulating transgene expression in genetically-manipulated organisms, for example to promote differentiation, de-differentiation, or any unidirectional developmental shift of a target cell which requires the time-specific expression of a particular gene. The invention is particularly suited to the promotion of specific organogeneses in plants using organogenesis-promoting transgenes, wherein the organs which subsequently develop in said plants are genetically transformed with a desired gene but lack organogenesis-promoting transgenes.

    ANTISENSE BASE SEQUENCES
    80.
    发明公开
    ANTISENSE BASE SEQUENCES 失效
    反义，SEQUENZEN

    公开(公告)号：EP0921195A1

    公开(公告)日：1999-06-09

    申请号：EP98905812.8

    申请日：1998-03-10

    申请人： Japan Tobacco Inc.

    发明人： MARUTA, Yoshiyuki, Japan Tobacco Inc. ,  SAITO, Hideaki, pan Tobacco Inc.

    IPC分类号： C12N15/29 ,  C12N15/63 ,  C12N5/10 ,  A01H1/00 ,  A01H5/00

    CPC分类号： C12N15/113 ,  A61K38/00 ,  C07K2319/00 ,  C12N15/8242 ,  C12N15/8251 ,  C12N2310/111

    摘要： The present invention provides a novel method to enhance an ability to suppress in vivo protein synthesis in a method of suppressing synthesis by use of an antisense nucleotide sequence. More specifically, the present invention provides an antisense nucleotide sequence comprising two or more successive repeats of the structural gene of interest or a fragment thereof in the antisense direction, an expression vector comprising said antisense nucleotide sequence, a host transformed by said expression vector and a method for suppressing the expression of a protein by use of said antisense nucleotide sequence.

    摘要翻译： 本发明提供了通过使用反义核苷酸序列抑制合成的方法来提高抑制体内蛋白质合成的能力的新方法。 更具体地说，本发明提供反义核苷酸序列，该反义核苷酸序列包含反义方向的目的结构基因或其片段的两个或多个连续重复序列，包含所述反义核苷酸序列的表达载体，由所述表达载体转化的宿主和 通过使用所述反义核苷酸序列抑制蛋白质表达的方法。