公开(公告)号：EP3077514A1

公开(公告)日：2016-10-12

申请号：EP14806626.9

申请日：2014-12-03

申请人： Novozymes A/S

发明人： HANSEN, Bjarne G. ,  OLSEN, Carsten, Lillelund ,  VIND, Jesper

IPC分类号： C12N15/65 ,  C12N15/80 ,  C12N15/90

CPC分类号： C12N15/902 ,  C12N15/80

摘要： The present invention relates to a method for site-specific chromosomal integration of a gene library in a filamentous fungal host cell and a polynucleotide construct suitable for this purpose.

摘要翻译： 本发明涉及丝状真菌宿主细胞中基因文库的位点特异性染色体整合方法和适用于该目的的多核苷酸构建体。

公开(公告)号：EP2683732B1

公开(公告)日：2016-08-24

申请号：EP12719604.6

申请日：2012-03-12

申请人： DSM IP Assets B.V.

发明人： BOVENBERG, Roelof Ary Lans ,  KIEL, Jan Andries Kornelis Willem ,  WENZEL, Thibaut José ,  LOS, Alrik Pieter

IPC分类号： C07K14/385 ,  C12N15/65 ,  C12N15/80

CPC分类号： C12N15/80 ,  C07K14/385 ,  C12N15/65

公开(公告)号：EP3027646A1

公开(公告)日：2016-06-08

申请号：EP14753158.6

申请日：2014-07-29

申请人： Novartis AG

发明人： ASSARAF, Yehuda G. ,  JOSTOCK, Thomas ,  KNOPF, Hans-Peter

IPC分类号： C07K14/705 ,  C12N15/65 ,  C12N15/69 ,  C12N15/85

CPC分类号： C12N15/65 ,  C07K14/705 ,  C07K16/00 ,  C12N15/69 ,  C12N15/85 ,  C12N2800/40

摘要： The disclosure pertains to an expression vector or a combination of at least two expression vectors comprising: a) a polynucleotide encoding a mutated folate receptor as selectable marker, wherein the mutated folate receptor has a decreased folate binding affinity compared to the wild type folate receptor; b) at least one polynucleotide encoding a polypeptide of interest, wherein when said expression vector or combination of at least two expression vectors is introduced into a host cell, the polypeptide of interest is secreted from said host cell. Also provided are suitable host cells, selection methods and methods for producing polypeptides with high yield.

公开(公告)号：EP2844736A4

公开(公告)日：2015-11-11

申请号：EP13784697

申请日：2013-05-06

申请人： UNIV HONG KONG

发明人： HUANG JIANDONG ,  YU BIN ,  YANG MEI ,  SHI LEI

IPC分类号： C12N1/21 ,  A61K39/108 ,  A61K39/112 ,  A61K48/00 ,  A61P35/00 ,  A61P35/04 ,  C12N15/113 ,  C12N15/65 ,  C12R1/185 ,  C12R1/19 ,  C12R1/42

CPC分类号： C12N15/74 ,  A61K35/74 ,  A61K38/00 ,  A61K39/00 ,  C12N15/70 ,  C12R1/19

摘要： Described herein is a method of treatment of cancer or tumor using a modified bacteria or composition comprising the modified bacteria. In certain embodiments, the method is in combination with other treatment. In certain embodiments, the treatment is chemotherapy, radiation therapy, gene therapy, surgery or a combination thereof. The method makes modified facultative anaerobic bacteria into a conditional obligate anaerobe. The modified bacteria are strictly hypoxia regulated and comprise an essential gene expressing cassette. The vectors of this method comprise the essential gene expressing cassette. Also described herein are therapeutic and prophylactic compositions comprising the modified bacteria. The therapeutic and prophylactic compositions contain a purified form of the modified bacteria, while in certain embodiments, they do not contain other strain of microorganisms. The modified bacteria grow within the solid tumor/cancer, retarding its growth and are rapidly eliminated from normal tissues. The solid tumor/cancer includes breast cancer, liver cancer or neuroblastoma.

摘要翻译： 描述中是使用改良的细菌或组合物，包括经修饰的细菌治疗的癌症或肿瘤的方法。 在某些实施方案中，该方法是在与其他治疗的组合。 在某些实施方案中，所述治疗是化学疗法，放射疗法，基因疗法，外科手术或它们的组合。 的方法使得改性兼性厌氧细菌进入一个条件专厌氧的。 修改后的细菌被严格限制和必需的基因表达盒缺氧包含。 该方法的载体包含表达盒中的必需基因。 如此描述的是治疗和预防了包含修改后的细菌。 的治疗和预防的组合物包含经修饰的细菌的纯化形式，而在某些实施方案中，它们不包含微生物的其他菌株。 经修饰的细菌生长的实体肿瘤/癌症中，延迟其生长和正在迅速地从正常组织中消除。 实体肿瘤/癌症包括乳腺癌，肝癌或神经母细胞瘤。

公开(公告)号：EP2582793A4

公开(公告)日：2014-05-14

申请号：EP11796365

申请日：2011-06-15

申请人： CELLULAR DYNAMICS INT INC

发明人： NUWAYSIR EMILE ,  KENDRICK-PARKER CHRIS ,  SEAY NICHOLAS

IPC分类号： C12N5/074 ,  C12N5/10 ,  C12N15/65 ,  C12N15/85 ,  C12Q1/02 ,  G01N33/15

CPC分类号： G01N33/5023 ,  G01N33/5014 ,  G01N33/5041 ,  G01N33/5073

公开(公告)号：EP2723872A1

公开(公告)日：2014-04-30

申请号：EP12729154.0

申请日：2012-06-25

申请人： Algenol Biofuels Inc.

发明人： DUEHRING, Ulf ,  LEE, James ,  BAIER, Kerstin

IPC分类号： C12N15/74 ,  C12N15/65 ,  C12P5/02 ,  C12P7/06 ,  C12P5/00

CPC分类号： C12N15/8243 ,  C12N15/74 ,  C12P5/007 ,  C12P5/026 ,  C12P7/065 ,  Y02E50/17

摘要： One embodiment of the invention provides a genetically enhanced cyanobacterium producing a first chemical compound comprising: -a genetically enhanced genome with a first gene inactivation in a first essential or conditionally essential gene of the cyanobacterium, and -a first extrachromosomal plasmid harboring the first essential or conditionally essential gene and at least one first production gene for production of the first chemical compound, -wherein the cyanobacterium lacks a functional gene conferring biocide resistance. These cyanobacteria can produce a first valuable chemical compound in long-term cultures without the need to employ genes conferring biocide resistance.

公开(公告)号：EP2430164A2

公开(公告)日：2012-03-21

申请号：EP10775323.8

申请日：2010-05-10

申请人： Pfenex, Inc.

发明人： RETALLACK, Diane, M. ,  CHEW, Lawrence, C. ,  SQUIRES, Charles, H.

IPC分类号： C12N15/65 ,  C12P21/04

CPC分类号： C12N15/65 ,  C12N15/63 ,  C12N15/78 ,  C12P21/02

摘要： Provided herein are methods and compositions for expression of a nucleic acid construct comprising nucleic acids encoding a) a recombinant polypeptide, and b) a prototrophy-restoring enzyme in a host cell that is auxotrophic for at least one metabolite. In various embodiments, the host cell is auxotrophic for a nitrogenous base compound or an amino acid. The invention involves introducing an analogue into the growth media for the host cell such that the analogue is incorporated into the recombinant polypeptide or a nucleic acid coding sequence thereof. In various embodiments, the compositions and methods disclosed herein result in improved recombinant protein expression compared to expression of recombinant protein in an antibiotic selection system, or compared to expression of the recombinant protein in an expression system that lacks a metabolite analogue.

公开(公告)号：EP2418282A2

公开(公告)日：2012-02-15

申请号：EP10758995.4

申请日：2010-03-30

申请人： Celltrion, Inc.

发明人： CHO, MyungSam ,  CHANG, Min Seok ,  KIM, Jong-Mook ,  LEE, HyunJoo ,  SONG, Yoo Cheol ,  KIM, ManSu

IPC分类号： C12N15/65 ,  C12N15/64 ,  C12N15/52

CPC分类号： C12N15/65 ,  C12N15/1086 ,  C12N15/69 ,  C12N15/85 ,  C12N2800/24 ,  C12N2830/46 ,  C12N2830/50 ,  C12P21/02

摘要： The present invention relates to a method of selecting high producer clones by using an expression vector, the expression vector comprising: (i) a gene expression cassette comprising a selectable marker gene to which polyA has been inoperably linked; and (ii) a gene expression cassette which encodes a recombinant protein of interest and to which polyA has been operably linked. According to the invention, high producer clones can be selected from cell populations at least 10 times fewer than in the existing methods of selecting cell lines. Particularly, high producer clones can be selected using a low concentration of MTX compared to a conventional stepwise gene amplification strategy which comprises carrying out multiple amplification steps while increasing the concentration of MTX. Accordingly, the development period of cell lines can be shortened and the labor and cost required for selection of high-productivity cell clones can be reduced, whereby more efficient production of proteins is possible even when general selectable marker genes other than MTX are used.

公开(公告)号：EP1608757B1

公开(公告)日：2011-12-14

申请号：EP04723149.3

申请日：2004-03-25

申请人： The Hong Kong Polytechnic University

发明人： WONG, Kwokyin, Hong Kong Polytech. Univ. ,  LEUNG, Thomas,Y , Hong Kong Polytech. U./Bio&Chem. ,  CHAN, Pakho, Hong Kong Polytech. U./Bio&Chem. Tec.

IPC分类号： C12N15/55 ,  C12N15/65 ,  C07K14/00 ,  C07K1/13 ,  G01N33/68 ,  A61P31/04

CPC分类号： C12N9/86 ,  G01N33/573

摘要： A tool that can screen bacteria for β-lactamases against a panel of various antibiotics is desirable. A biosensor incorporating an indicator molecule into β-lactamases may achieve this purpose, but it requires that the attached indicator molecule must not impair the binding affinity of the protein to a great extent to provide a higher sensitivity. A modified β-lactamases with a residue on the Ω-loop or outside the Ω-loop but close to the active site of β-lactamase being replaced by a reactive residue is developed in this invention.

公开(公告)号：EP2310510A2

公开(公告)日：2011-04-20

申请号：EP09798313.4

申请日：2009-07-17

申请人： Medigen, Inc.

发明人： PUSHKO, Peter ,  LUKASHEVICH, Igor

IPC分类号： C12N15/65 ,  A61K48/00 ,  C12N5/00

CPC分类号： A61K39/193 ,  A61K39/12 ,  A61K2039/53 ,  C12N7/00 ,  C12N15/85 ,  C12N2770/24134 ,  C12N2770/24161 ,  C12N2770/36134 ,  C12N2770/36161 ,  Y02A50/388

摘要： Described herein are iDNA vectors and vaccines and methods for using the same. The iDNA generates live attenuated vaccines in eukaryotic cells in vitro or in vivo for pathogenic RNA viruses, particularly yellow fever virus and Venezuelan equine encephalitis virus. When iDNA is injected into the vaccine recipient, RNA of live attenuated virus is generated by in vivo transcription in the recipient's tissues. This initiates production of progeny attenuated viruses in the tissues of the vaccine recipient, as well as elicitation of an effective immune response protecting against wild-type, non-attenuated virus.