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    RECOMBINANT BACTERIAL HOST CELL FOR PROTEIN EXPRESSION
    5.
    发明公开
    RECOMBINANT BACTERIAL HOST CELL FOR PROTEIN EXPRESSION 审中-公开
    复方巴斯伯林紫苏锌蛋白表达

    公开(公告)号：EP2850100A1

    公开(公告)日：2015-03-25

    申请号：EP13723086.8

    申请日：2013-05-13

    申请人： UCB Pharma, S.A.

    发明人： BASSETT, Philip Jonathan ,  HUMPHREYS, David Paul ,  PATEL, Pareshkumar Manjibhai

    IPC分类号： C07K16/24 ,  C12N15/70

    CPC分类号： C12P21/00 ,  C07K16/283 ,  C07K2317/10 ,  C12N9/52 ,  C12N15/70 ,  C12P21/02

    摘要： The present disclosure relates to a recombinant gram-negative bacterial cell comprising: a.) a mutant spr gene encoding a spr protein having a mutation at one or more amino acids selected from D133, H145, H157, N31, R62, I70, Q73, C94, S95, V98, Q99, R100, L108, Y115, V135, L136, G140, R144 and G147 and b.) a gene capable of expressing or overexpressing one or more proteins capable of facilitating protein folding, such as FkpA, Skp, SurA, PPiA and PPiD, wherein the cell has reduced Tsp protein activity compared to a wild-type cell, methods employing the cells, use of the cells in the expression of proteins in particular antibodies, such as anti Fc Rn antibodies and proteins made by the methods described herein.

    摘要翻译： 本公开涉及重组革兰氏阴性细菌细胞，其包含：a）编码具有在一个或多个选自D133，H145，H157，N31，R62，I70，Q73， C94，S95，V98，Q99，R100，L108，Y115，V135，L136，G140，R144和G147，以及b）能够表达或过表达能够促进蛋白质折叠的一种或多种蛋白质的基因，例如FkpA，Skp， SurA，PPiA和PPiD，其中与野生型细胞相比，细胞具有降低的Tsp蛋白活性，使用细胞的方法，使用细胞表达特定抗体的蛋白质，例如抗Fc Rn抗体和由 本文描述的方法。