摘要:
The invention is directed towards a cell culture apparatus, which comprises; at least two cell culture units, each having cell culture chambers, a filling port having an inlet, an overflow outlet and a cell culture outlet in fluid communication with the at least two culture chambers, to allow fluid introduced through the inlet to enter the chambers through the cell culture outlet, wherein the filling port is configured to cause fluid introduced into the inlet to flow out the overflow outlet when the culture chambers are filled with fluid, wherein the overflow outlet of a first cell culture unit is fluidly coupled to the inlet of a second cell culture unit.
摘要:
The invention is directed towards a cell culture apparatus, which comprises; at least two cell culture units, each having cell culture chambers, a filling port having an inlet, an overflow outlet and a cell culture outlet in fluid communication with the at least two culture chambers, to allow fluid introduced through the inlet to enter the chambers through the cell culture outlet, wherein the filling port is configured to cause fluid introduced into the inlet to flow out the overflow outlet when the culture chambers are filled with fluid, wherein the overflow outlet of a first cell culture unit is fluidly coupled to the inlet of a second cell culture unit.
摘要:
A cell culture apparatus includes a cell culture chamber formed by a first major surface, an opposing second major surface spaced apart from the first major surface, and a first side wall around the first chamber and extending between the first and second major surfaces. A portion of the first sidewall proximate the first major surface comprises an infrared absorbent material, and a portion the first sidewall proximate the second major surface is formed from substantially non-infrared absorbent material. The first major surface is formed by a gas permeable polymeric film that is impermeable to cell culture liquid.
摘要:
A device for minimizing the formation of bubbles or foam in cell culture is disclosed. The device has a manifold which directs the inflow of cells and cell culture media into a cell culture vessel so as to allow for displaced air or gas to vent from the cell culture vessel without mixing with the incoming cell culture media, thereby preventing the mixing of air and cell culture media and minimizing the formation of bubbles or foam inside the cell culture vessel.
摘要:
A method of manufacture and assembly of multiwell plates employing targeted radiation at an interface in order to achieve bonding is disclosed. The method accommodates glass and polymer attachment as well as polymer to polymer attachment. The resulting plates have unique flatness and optical properties. The multiwell test plate (10) includes a peripheral skirt (12) and an upper surface (13) having an array of wells (14) and top rim (16).
摘要:
A multiwell plate (100) is described herein that can be used in cell-based applications and is made from a plastic upper plate (200) which forms the sidewalls of one or more wells (160) and a glass lower plate (220) which forms the bottom walls of the wells. The plastic upper plate and glass lower plate are attached and bound to one another by a cationically photocured adhesive (280). A preferred cationically photcured adhesive includes: (1) one or more photocationally polymerizable epoxy and/or oxetane functional resins; (2) a low fluorescing cationic photoinitiator; and (3) a low fluorescing photosensitizer if the cationic photoinitiator does not have an adequate absorption at a wavelength >280nm to initiate cure. Also described herein is a method for making such multiwell plates.
摘要:
A device and method for detecting a signal from an analytical sample is provided. The device comprises a platen with a first and second surface, a number of collection structures extending from the second surface. Each collection structure has a capillary channel running along a common axis as the collection structure from the platen to a terminal end of the structure. Within the capillary channel are sample analytes of interest. On the first surface is disposed a number of optical elements, corresponding to each collection structure, each centered on a capillary channel. Each collection structure has a geometry that can concentrate an optical emission from within the capillary channel, along the entire length of the capillary channel, towards the first surface and optical elements, to a detection device, by means of either mirrored surface reflection or total internal reflection. The collection structure may also direct a light beam into the capillary channel. The present device can be used for non-imaging optical applications from the generated signal.
摘要:
A method of manufacture and assembly of multiwell plates employing targeted radiation at an interface in order to achieve bonding is disclosed. The method accommodates glass and polymer attachment as well as polymer to polymer attachment. The resulting plates have unique flatness and optical properties. The multiwell test plate (10) includes a peripheral skirt (12) and an upper surface (13) having an array of wells (14) and top rim (16).