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公开(公告)号：EP2155880A1
公开(公告)日：2010-02-24
申请号：EP08772802.8
申请日：2008-06-13
发明人： D'AOUST, Marc-Andre , MARQUET-BLOUIN, Estelle , BARDOR, Muriel , BUREL, Carole , FAYE, Loic , LEROUGE, Patrice , VEZINA, Louis-Philippe , GOMORD, Véronique , AQUIN, Stéphanie , RIHOUEY, Christophe , PACCALET, Thomas , SOURROUILLE, Christophe
IPC分类号： C12N15/62 , A01H5/00 , A01H5/10 , C07K19/00 , C12N15/54 , C12N15/82 , C12N5/10 , C12N9/10 , C12P21/02 , C12P21/08
CPC分类号： C12N15/8257 , C12N9/1051 , C12N15/8216 , C12N15/8218 , C12N15/8245 , C12N15/8258
摘要： A method for synthesizing a protein of interest with a modified N-glycosylation profile within a plant, a portion of a plant, or a plant cell is provided. The method comprises co-expressing within a plant a nucleotide sequence encoding a first nucleotide sequence encoding a hybrid protein (GNT1-GalT) comprising a CTS domain of N-acetylglucosaminyl transferase (GNT1) fused to a catalytic domain of beta-1,4galactosyltransferase (GalT), the first nucleotide sequence operatively linked with a first regulatory region that is active in the plant, and a second nucleotide sequence for encoding the protein of interest, the second nucleotide sequence operatively linked with a second regulatory region that is active in the plant. The first and second nucleotide sequences are co-expressed to synthesize a protein of interest comprising glycans with the modified N-glycosylation profile within the plant, the portion of the plant, or the plant cell.
摘要翻译： 提供了在植物，植物的一部分或植物细胞内合成具有修饰的N-糖基化分布的目的蛋白质的方法。 所述方法包括在植物内共表达编码编码包含N-乙酰葡糖胺基转移酶（GNT1）的CTS结构域的杂合蛋白（GNT1-GalT）的第一核苷酸序列的核苷酸序列，所述CTS结构域与β-1,4半乳糖基转移酶的催化结构域 GalT），第一核苷酸序列与在植物中有活性的第一调控区有效连接，第二核苷酸序列用于编码感兴趣的蛋白质，第二核苷酸序列与在植物中有活性的第二调控区有效连接 。 第一和第二核苷酸序列共表达以合成包含植物内植物，植物部分或植物细胞内具有修饰的N-糖基化谱的聚糖的目的蛋白质。
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公开(公告)号：EP2155880B1
公开(公告)日：2016-08-10
申请号：EP08772802.8
申请日：2008-06-13
发明人： D'AOUST, Marc-Andre , MARQUET-BLOUIN, Estelle , BARDOR, Muriel , BUREL, Carole , FAYE, Loic , LEROUGE, Patrice , VEZINA, Louis-Philippe , GOMORD, Véronique , AQUIN, Stéphanie , RIHOUEY, Christophe , PACCALET, Thomas , SOURROUILLE, Christophe
IPC分类号： C12N15/62 , A01H5/00 , A01H5/10 , C07K19/00 , C12N15/54 , C12N15/82 , C12N5/10 , C12N9/10 , C12P21/02 , C12P21/08
CPC分类号： C12N15/8257 , C12N9/1051 , C12N15/8216 , C12N15/8218 , C12N15/8245 , C12N15/8258
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公开(公告)号：EP1987137B1
公开(公告)日：2011-06-22
申请号：EP07710612.8
申请日：2007-02-09
发明人： PACCALET, Thomas , BARDOR, Muriel , RIHOUEY, Christophe , GOMORD, Véronique , FAYE, Loïc , LEROUGE, Patrice , AQUIN, Stéphanie , VEZINA, Louis-Philippe , D'AOUST, Marc-André
IPC分类号： C12N15/82 , C12N15/54 , C12N15/60 , C12N5/04 , C12N5/10 , C12N9/88 , C12N9/90 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8245 , C12N9/1085 , C12N9/88 , C12N15/8257 , C12P19/26 , C12P21/005 , C12Y205/01057 , C12Y401/03003
摘要： A method of synthesizing sialic acid in plants, and plants capable of synthesizing sialic acid is provided. Furthermore, a method of producing sialylated protein in a plant is also provided. The method to synthesize sialic acid comprises providing a plant comprising a nucleotide sequence encoding N-acetyl neuraminic acid (Neu5Ac) synthase or Neu5Ac lyase, and expressing the nucleotide sequence thereby synthesizing sialic acid. The plant may also co-express a nucleotide sequence encoding one or more than one of an epimerase, a CMP-Neu5Ac synthase, a CMP-Neu5Ac transporter and a sialyltransferase.
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公开(公告)号：EP1987137A1
公开(公告)日：2008-11-05
申请号：EP07710612.8
申请日：2007-02-09
发明人： PACCALET, Thomas , BARDOR, Muriel , RIHOUEY, Christophe , GOMORD, Véronique , FAYE, Loïc , LEROUGE, Patrice , AQUIN, Stéphanie , VEZINA, Louis-Philippe , D'AOUST, Marc-André
IPC分类号： C12N5/10 , A01H1/00 , A01H5/00 , A01H5/10 , A61K38/14 , C12N15/54 , C12N15/60 , C12N15/82 , C12N5/04 , C12N9/88 , C12N9/90 , C12P19/02 , C12P21/00 , C12N9/10
CPC分类号： C12N15/8245 , C12N9/1085 , C12N9/88 , C12N15/8257 , C12P19/26 , C12P21/005 , C12Y205/01057 , C12Y401/03003
摘要： A method of synthesizing sialic acid in plants, and plants capable of synthesizing sialic acid is provided. Furthermore, a method of producing sialylated protein in a plant is also provided. The method to synthesize sialic acid comprises providing a plant comprising a nucleotide sequence encoding N-acetyl neuraminic acid (Neu5Ac) synthase or Neu5Ac lyase, and expressing the nucleotide sequence thereby synthesizing sialic acid. The plant may also co-express a nucleotide sequence encoding one or more than one of an epimerase, a CMP-Neu5Ac synthase, a CMP-Neu5Ac transporter and a sialyltransferase.
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