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公开(公告)号：EP2155880A1
公开(公告)日：2010-02-24
申请号：EP08772802.8
申请日：2008-06-13
发明人： D'AOUST, Marc-Andre , MARQUET-BLOUIN, Estelle , BARDOR, Muriel , BUREL, Carole , FAYE, Loic , LEROUGE, Patrice , VEZINA, Louis-Philippe , GOMORD, Véronique , AQUIN, Stéphanie , RIHOUEY, Christophe , PACCALET, Thomas , SOURROUILLE, Christophe
IPC分类号： C12N15/62 , A01H5/00 , A01H5/10 , C07K19/00 , C12N15/54 , C12N15/82 , C12N5/10 , C12N9/10 , C12P21/02 , C12P21/08
CPC分类号： C12N15/8257 , C12N9/1051 , C12N15/8216 , C12N15/8218 , C12N15/8245 , C12N15/8258
摘要： A method for synthesizing a protein of interest with a modified N-glycosylation profile within a plant, a portion of a plant, or a plant cell is provided. The method comprises co-expressing within a plant a nucleotide sequence encoding a first nucleotide sequence encoding a hybrid protein (GNT1-GalT) comprising a CTS domain of N-acetylglucosaminyl transferase (GNT1) fused to a catalytic domain of beta-1,4galactosyltransferase (GalT), the first nucleotide sequence operatively linked with a first regulatory region that is active in the plant, and a second nucleotide sequence for encoding the protein of interest, the second nucleotide sequence operatively linked with a second regulatory region that is active in the plant. The first and second nucleotide sequences are co-expressed to synthesize a protein of interest comprising glycans with the modified N-glycosylation profile within the plant, the portion of the plant, or the plant cell.
摘要翻译： 提供了在植物，植物的一部分或植物细胞内合成具有修饰的N-糖基化分布的目的蛋白质的方法。 所述方法包括在植物内共表达编码编码包含N-乙酰葡糖胺基转移酶（GNT1）的CTS结构域的杂合蛋白（GNT1-GalT）的第一核苷酸序列的核苷酸序列，所述CTS结构域与β-1,4半乳糖基转移酶的催化结构域 GalT），第一核苷酸序列与在植物中有活性的第一调控区有效连接，第二核苷酸序列用于编码感兴趣的蛋白质，第二核苷酸序列与在植物中有活性的第二调控区有效连接 。 第一和第二核苷酸序列共表达以合成包含植物内植物，植物部分或植物细胞内具有修饰的N-糖基化谱的聚糖的目的蛋白质。
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公开(公告)号：EP1987137B1
公开(公告)日：2011-06-22
申请号：EP07710612.8
申请日：2007-02-09
发明人： PACCALET, Thomas , BARDOR, Muriel , RIHOUEY, Christophe , GOMORD, Véronique , FAYE, Loïc , LEROUGE, Patrice , AQUIN, Stéphanie , VEZINA, Louis-Philippe , D'AOUST, Marc-André
IPC分类号： C12N15/82 , C12N15/54 , C12N15/60 , C12N5/04 , C12N5/10 , C12N9/88 , C12N9/90 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8245 , C12N9/1085 , C12N9/88 , C12N15/8257 , C12P19/26 , C12P21/005 , C12Y205/01057 , C12Y401/03003
摘要： A method of synthesizing sialic acid in plants, and plants capable of synthesizing sialic acid is provided. Furthermore, a method of producing sialylated protein in a plant is also provided. The method to synthesize sialic acid comprises providing a plant comprising a nucleotide sequence encoding N-acetyl neuraminic acid (Neu5Ac) synthase or Neu5Ac lyase, and expressing the nucleotide sequence thereby synthesizing sialic acid. The plant may also co-express a nucleotide sequence encoding one or more than one of an epimerase, a CMP-Neu5Ac synthase, a CMP-Neu5Ac transporter and a sialyltransferase.
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公开(公告)号：EP2155880B1
公开(公告)日：2016-08-10
申请号：EP08772802.8
申请日：2008-06-13
发明人： D'AOUST, Marc-Andre , MARQUET-BLOUIN, Estelle , BARDOR, Muriel , BUREL, Carole , FAYE, Loic , LEROUGE, Patrice , VEZINA, Louis-Philippe , GOMORD, Véronique , AQUIN, Stéphanie , RIHOUEY, Christophe , PACCALET, Thomas , SOURROUILLE, Christophe
IPC分类号： C12N15/62 , A01H5/00 , A01H5/10 , C07K19/00 , C12N15/54 , C12N15/82 , C12N5/10 , C12N9/10 , C12P21/02 , C12P21/08
CPC分类号： C12N15/8257 , C12N9/1051 , C12N15/8216 , C12N15/8218 , C12N15/8245 , C12N15/8258
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公开(公告)号：EP1987137A1
公开(公告)日：2008-11-05
申请号：EP07710612.8
申请日：2007-02-09
发明人： PACCALET, Thomas , BARDOR, Muriel , RIHOUEY, Christophe , GOMORD, Véronique , FAYE, Loïc , LEROUGE, Patrice , AQUIN, Stéphanie , VEZINA, Louis-Philippe , D'AOUST, Marc-André
IPC分类号： C12N5/10 , A01H1/00 , A01H5/00 , A01H5/10 , A61K38/14 , C12N15/54 , C12N15/60 , C12N15/82 , C12N5/04 , C12N9/88 , C12N9/90 , C12P19/02 , C12P21/00 , C12N9/10
CPC分类号： C12N15/8245 , C12N9/1085 , C12N9/88 , C12N15/8257 , C12P19/26 , C12P21/005 , C12Y205/01057 , C12Y401/03003
摘要： A method of synthesizing sialic acid in plants, and plants capable of synthesizing sialic acid is provided. Furthermore, a method of producing sialylated protein in a plant is also provided. The method to synthesize sialic acid comprises providing a plant comprising a nucleotide sequence encoding N-acetyl neuraminic acid (Neu5Ac) synthase or Neu5Ac lyase, and expressing the nucleotide sequence thereby synthesizing sialic acid. The plant may also co-express a nucleotide sequence encoding one or more than one of an epimerase, a CMP-Neu5Ac synthase, a CMP-Neu5Ac transporter and a sialyltransferase.
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公开(公告)号：EP2635684B1
公开(公告)日：2016-10-26
申请号：EP11837364.6
申请日：2011-11-03
申请人： Medicago Inc.
IPC分类号： C07K14/005 , C12N15/82
CPC分类号： C07K14/005 , C12N15/8203 , C12N15/8216 , C12N15/8221 , C12N15/8257 , C12N15/8258 , C12N2750/12022 , C12N2760/16051 , C12N2770/18022 , C12N2820/60 , C12N2830/60
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公开(公告)号：EP2814941A1
公开(公告)日：2014-12-24
申请号：EP13748545.4
申请日：2013-02-15
申请人： Medicago Inc.
CPC分类号： A01G7/06 , A01G7/00 , A01G22/00 , C12N15/8205
摘要： It is provided a plant infiltration device for infiltrating plants with an inoculum and method of using same, the device comprising a frame having one or more tray racks adapted for receiving at least one tray of plants to be infiltrated; an automated manipulation mechanism mounted to the frame and operable to displace the plant trays within the device; a plurality of inoculum reservoirs mounted to the frame and containing one or more inoculum fluids; an infiltration tank mounted to the frame and within which the plant trays are received when disposed in the infiltration position; and a vacuum generating device in communication with the infiltration tank to apply a negative pressure within the infiltration tank, thereby opening pores of the plant leaves immersed in the inoculum within the infiltration tank and causing the inoculum fluid to infiltrate into the plant leaves.
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公开(公告)号：EP2445928A1
公开(公告)日：2012-05-02
申请号：EP10791119.0
申请日：2010-06-25
申请人： Medicago Inc.
发明人： COUTURE, Manon , DARGIS,Michele , LAVOIE, Pierre-Olivier , VEZINA, Louis-Philippe , D'AOUST, Marc-Andre
IPC分类号： C07K14/11 , A01H5/00 , A61K39/145 , A61P31/16 , C12N15/44 , C12N15/82 , C12N7/00 , C12N7/01 , C07K19/00
CPC分类号： A61K39/145 , A61K39/12 , A61K2039/5258 , C07K14/005 , C07K2319/00 , C07K2319/02 , C12N15/8257 , C12N2760/16122 , C12N2760/16123 , C12N2760/16134 , C12N2760/16222 , C12N2760/16223 , C12N2760/16234
摘要： A method for synthesizing chimeric influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of chimeric influenza HA in a plant or a portion of a plant. The invention is also directed towards a VLP comprising chimeric influenza HA protein and plants lipids. The invention is also directed to a nucleic acid encoding chimeric influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.
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公开(公告)号：EP2480560B1
公开(公告)日：2018-02-21
申请号：EP10818191.8
申请日：2010-09-21
申请人： Medicago Inc.
CPC分类号： A61K39/145 , A61K39/12 , A61K2039/517 , A61K2039/5258 , C12N7/00 , C12N15/8203 , C12N15/8257 , C12N15/8258 , C12N2760/16123 , C12N2760/16134
摘要： Methods of preparing plant-derived virus like particles (VLPs) are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized VLPs, producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter, and recovering the apoplast fraction. The apoplast fraction comprises plant-derived VLPs. Alternatively, VLPs may be obtained from plant or plant matter comprising plant-derived VLPs by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction, and the plant-derived VLPs are recovered from the filtered fraction.
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公开(公告)号：EP3228627A1
公开(公告)日：2017-10-11
申请号：EP17152741.9
申请日：2010-06-25
申请人： Medicago Inc.
发明人： COUTURE, Manon , DARGIS, Michele , LAVOIE, Pierre-Olivier , VEZINA, Louis-Philippe , D'AOUST, Marc-Andre
IPC分类号： C07K14/11 , A01H5/00 , A61K39/145 , A61P31/16 , C12N15/44 , C12N15/82 , C12N7/00 , C12N7/01 , C07K19/00
CPC分类号： A61K39/145 , A61K39/12 , A61K2039/5258 , C07K14/005 , C07K2319/00 , C07K2319/02 , C12N15/8257 , C12N2760/16122 , C12N2760/16123 , C12N2760/16134 , C12N2760/16222 , C12N2760/16223 , C12N2760/16234
摘要： A method for synthesizing chimeric influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of chimeric influenza HA in a plant or a portion of a plant. The invention is also directed towards a VLP comprising chimeric influenza HA protein and plants lipids. The invention is also directed to a nucleic acid encoding chimeric influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.
摘要翻译： 提供了在植物或植物的一部分内合成嵌合流感病毒样颗粒（VLP）的方法。 该方法涉及在植物或植物的一部分中表达嵌合流感HA。 本发明还涉及包含嵌合流感HA蛋白和植物脂质的VLP。 本发明还涉及编码嵌合流感HA的核酸以及载体。 VLP可用于配制流感疫苗，或可用于丰富现有疫苗。
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