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公开(公告)号：EP2871239B1
公开(公告)日：2018-08-29
申请号：EP13813664.3
申请日：2013-06-26
申请人： Takara Bio Inc.
IPC分类号： C12N15/113 , C12N5/10 , C12N15/864 , C12N7/00 , C12N15/09
CPC分类号： C12N15/86 , C12N5/10 , C12N7/00 , C12N15/09 , C12N15/111 , C12N15/64 , C12N2310/141 , C12N2330/51 , C12N2501/65 , C12N2750/14143 , C12N2750/14151 , C12P19/34
摘要： According to the present invention, an AAV vector having a higher titer compared with those of conventional ones can be produced using a cell into which a nucleic acid capable of expressing miRNA is introduced artificially. An AAV vector produced using the cell and a composition containing the viral vector as an active ingredient are very useful as gene transfer means in the studies or clinical practice of gene therapies.
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公开(公告)号：EP3243904A4
公开(公告)日：2018-06-27
申请号：EP16735082
申请日：2016-01-08
申请人： TAKARA BIO INC
IPC分类号： C12N7/02 , C07K14/075 , C12N5/10 , C12N15/09
CPC分类号： C07K14/075 , C12N5/10 , C12N7/02 , C12N15/09
摘要： Provided is a method for producing non-enveloped viral particles, comprising a step for obtaining a fraction containing non-enveloped viral particles by removing precipitates which are produced in a step for adding a substance which reduces the solubility of proteins under acidic conditions and/or a substance which precipitates under acidic conditions to a neutral or basic sample containing non-enveloped viral particles, and acidifying the sample after the addition of the substance.
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公开(公告)号：EP2404997B1
公开(公告)日：2017-10-18
申请号：EP10748842.1
申请日：2010-03-05
申请人： Mie University , TAKARA BIO INC.
IPC分类号： C12N15/00 , A61K35/14 , A61P31/00 , A61P35/00 , A61P37/06 , C12N5/10 , C12N15/09 , A61K31/713 , A61K48/00 , C12N5/0783 , C12N15/113 , A61K35/12 , A61K35/17 , A61K39/00
CPC分类号： A61K35/17 , A61K2035/124 , A61K2039/5158 , C12N5/0636 , C12N15/113 , C12N2310/14 , C12N2501/48 , C12N2501/599 , C12N2310/531
摘要： Disclosed is a method for enhancing the function of a T cell, which is characterized by inhibiting the expression of programmed death-1 ligand 1 (PD-L1) and/or programmed death-1 ligand 2 (PD-L2) in the T cell. Also disclosed is a function-enhanced T cell which is produced by the function enhancement method. Further disclosed is a therapeutic agent comprising the function-enhanced T cell. The T cell can enhance an immune response to cancer, and is useful in an immunotherapy effective for cancer and the treatment or prevention of infectious diseases and autoimmune diseases.
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公开(公告)号：EP2975124A4
公开(公告)日：2016-11-30
申请号：EP14762697
申请日：2014-03-13
申请人： TAKARA BIO INC
CPC分类号： C12N9/22 , C12P19/34 , C12Q1/683 , C12Y301/21 , C12Q2521/301 , C12Q2521/514
摘要： Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.
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公开(公告)号：EP3020808A1
公开(公告)日：2016-05-18
申请号：EP14823074.1
申请日：2014-07-10
申请人： Takara Bio Inc.
发明人： SAKAMOTO, Shuhei , KAWANO, Yasuhiro , DODO, Katsuyuki , ENOKI, Tatsuji , YOSHIOKA, Hirofumi , TAKAKURA, Hikaru , MINENO, Junichi
CPC分类号： C12N7/00 , C12N2750/14151 , C12N15/86 , C12N2750/14143 , C12N2750/14043 , C12N2750/14122 , C12N2750/14142 , C12N7/02 , C12N2750/14141 , C12N2750/14351 , C12N2800/50 , C12N15/8673 , C12N2750/14352 , C12N2750/14152
摘要： The present invention provides a method for efficiently manufacturing a non-enveloped virus with high purity without laborious operation by cultivating cells having the ability to produce a non-enveloped virus and bringing the cells and an acidic solution into contact with each other. A non-enveloped virus vector manufactured by the method of the present invention and a composition having the non-enveloped viral vector as an active ingredient are very useful as gene transfer methods in the fields of basic research and clinical application gene therapy.
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公开(公告)号：EP2829606A4
公开(公告)日：2016-02-24
申请号：EP13763874
申请日：2013-03-14
申请人： TAKARA BIO INC
CPC分类号： C12N15/86 , A61K35/13 , A61K48/00 , C12N5/0018 , C12N7/00 , C12N2740/10041 , C12N2740/10051 , C12N2740/10052
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公开(公告)号：EP2940131A1
公开(公告)日：2015-11-04
申请号：EP13867056.7
申请日：2013-12-20
申请人： Takara Bio, Inc.
CPC分类号： C12N15/86 , C07K14/005 , C12N7/00 , C12N2750/14122 , C12N2750/14142 , C12N2750/14143
摘要： The invention provides an AAV particle containing an adeno-associated viral (AAV) capsid protein having an amino acid sequence selected from the group consisting of SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 24, and SEQ ID NO: 30 of the sequence listing; a nucleic acid that encodes this capsid protein; DNA containing this nucleic acid; a cell containing this DNA; and a method for producing this cell.
摘要翻译： 本发明提供一种含有腺病毒（AAV）衣壳蛋白的AAV颗粒，其具有选自SEQ ID NO：15，SEQ ID NO：16，SEQ ID NO：24和SEQ ID NO的氨基酸序列 ：序列表中的30个; 编码该衣壳蛋白的核酸; 含有该核酸的DNA; 含有该DNA的细胞; 以及该细胞的制造方法。
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公开(公告)号：EP2811020A1
公开(公告)日：2014-12-10
申请号：EP13743868.5
申请日：2013-01-24
申请人： Takara Bio, Inc.
CPC分类号： C12Q1/6848 , C12N9/1252 , C12N9/1276 , C12P19/34 , C12Q1/6844 , C12Y207/07007 , C12Q2527/125
摘要： Provided are the following: a method, for improving reactivity of an acid synthesis reaction, comprising a step for adding an ω-amino acid to a reaction solution; a composition, for a nucleic acid synthesis reaction, comprising DNA polymerase, reaction buffer, at least one primer, at least one deoxyribonucleotide triphosphate, and an ω-amino acid; and a reaction buffer, for a nucleic acid synthesis reaction, comprising an ω-amino acid.
摘要翻译： 本发明提供一种提高酸合成反应的反应性的方法，其包括向反应液中添加ω-氨基酸的工序， 用于核酸合成反应的组合物，其包含DNA聚合酶，反应缓冲液，至少一种引物，至少一种脱氧核糖核苷酸三磷酸和ω-氨基酸; 和用于核酸合成反应的包含ω-氨基酸的反应缓冲液。
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