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    GENES ENCODING CAROTENOID COMPOUNDS
    6.
    发明公开
    GENES ENCODING CAROTENOID COMPOUNDS 审中-公开
    编码类胡萝卜素化合物的基因

    公开(公告)号：EP1631660A2

    公开(公告)日：2006-03-08

    申请号：EP04816748.0

    申请日：2004-06-10

    申请人： E.I. DUPONT DE NEMOURS AND COMPANY

    发明人： CHENG, Qiong ,  SEDKOVA, Natalia ,  TAO, Luan

    IPC分类号： C12N1/00

    CPC分类号： C12N9/00 ,  C12N9/0004 ,  C12N9/001 ,  C12N9/0083 ,  C12N9/1051 ,  C12N9/1085 ,  C12N9/90 ,  C12N15/52 ,  C12N15/825 ,  C12P23/00 ,  C12Y205/01029 ,  Y02E50/343

    摘要： A unique carotenogenic biosynthetic gene cluster has been isolated from Panteoa agglomerans strain DC404, wherein the genetic organization of the cluster is crtE-idi-crtY-crtI-crtB-crtZ. The genes contained within this cluster encode geranylgeranyl pyrophosphate (GGPP) synthetase (CrtE), isopentenyl pyrophosphate isomerase (Idi), lycopene cyclase (CrtY), phytoene desaturase (CrtI), phytoene synthase (CrtB), and β-carotene hydroxylase (CrtZ). The gene cluster, genes and their products are useful for the conversion of farnesyl pyrophosphate to carotenoids. Vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes by recombinant DNA technology in transformed host organisms are disclosed.

    摘要翻译： 已经从Panteoa agglomerans菌株DC404中分离出独特的类胡萝卜素生物合成基因簇，其中该簇的遗传组织是crtE-idi-crtY-crtI-crtB-crtZ。 包含在该簇中的基因编码焦磷酸ge牛儿基ge牛儿基焦磷酸（GGPP）合成酶（CrtE），异戊烯焦磷酸异构酶（Idi），番茄红素环化酶（CrtY），八氢番茄红素去饱和酶（CrtI），八氢番茄红素合酶（CrtB）和β-胡萝卜素羟化酶（CrtZ） 。 基因簇，基因及其产物用于法尼基焦磷酸转化为类胡萝卜素。 公开了含有这些DNA片段的载体，含有载体的宿主细胞和通过重组DNA技术在转化的宿主生物体中产生这些酶的方法。

    NUCLEIC ACIDS ENCODING TAXUS GERANYLGERANYL DIPHOSPHATE SYNTHASE, AND METHODS OF USE
    8.
    发明公开
    NUCLEIC ACIDS ENCODING TAXUS GERANYLGERANYL DIPHOSPHATE SYNTHASE, AND METHODS OF USE 审中-公开
    NUCLEIC这对于TAXUS GERRANYLGERANYL二磷酸合成酶的编码和给药方法

    公开(公告)号：EP1124943A1

    公开(公告)日：2001-08-22

    申请号：EP99971845.5

    申请日：1999-10-27

    申请人： WASHINGTON STATE UNIVERSITY RESEARCH FOUNDATION

    发明人： CROTEAU, Rodney, B. ,  HEFNER, Jerry L.

    IPC分类号： C12N9/00 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04 ,  C07K1/00

    CPC分类号： C12N9/1085 ,  C12N15/8243 ,  C12Y205/01029

    摘要： A cDNA encoding geranylgeranyl diphosphate synthase from Canadian Yew (Taxus canadensis) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ NO:1) is provided which codes for the expression of geranylgeranyl diphosphate synthase (SEQ ID NO:2), from Canadian Yew (Taxus canadensis). In other aspects, replicable recombinant cloning vehicles are provided which code for geranylgeranyl diphosphate synthase, or for a base sequence sufficiently complementary to at least a portion of geranylgeranyl diphosphate synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding geranylgeranyl disphosphate synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant geranylgeranyl diphosphate synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant geranylgeranyl diphosphate synthase may be used to obtain expression or enhanced expression of geranylgeranyl diphosphate synthase in plants in order to enhance the production of geranylgeranyl diphosphate, or diterpenes derived therefrom, or may be otherwise employed for the regulation or expression of geranylgeranyl diphosphate synthase, or the production of its products.