公开(公告)号：EP3083965B1

公开(公告)日：2019-07-10

申请号：EP14811909.2

申请日：2014-12-12

申请人： BASF SE

发明人： JENEWEIN, Stefan ,  FELLE, Max Fabian ,  REYELT, Jan

IPC分类号： C07K14/245 ,  C12N15/70 ,  C12P21/00 ,  C12N15/64 ,  C12N15/68

公开(公告)号：EP2814964B1

公开(公告)日：2019-01-09

申请号：EP13705106.6

申请日：2013-02-15

申请人： CureVac AG

发明人： THESS, Andreas ,  SCHLAKE, Thomas ,  PROBST, Jochen

IPC分类号： C12N15/68 ,  A61K39/00 ,  A61K39/35 ,  C12N15/117 ,  C12N15/63 ,  C12N15/67 ,  C12N15/85

公开(公告)号：EP3164494B1

公开(公告)日：2018-05-02

申请号：EP15730138.3

申请日：2015-06-17

申请人： Wacker Chemie AG

发明人： ANTON, Andreas ,  JANOWSKI-EGLER, Monique ,  LIEBSCHER, Markus

IPC分类号： C12N15/68 ,  C12N15/72 ,  C12N15/73 ,  C12N15/90

CPC分类号： C12N9/1247 ,  C12N15/68 ,  C12N15/70 ,  C12N15/72 ,  C12N15/73 ,  C12Y207/07006

公开(公告)号：EP3156487A4

公开(公告)日：2018-01-10

申请号：EP15807551

申请日：2015-04-20

申请人： OURO FINO SAÚDE ANIMAL LTDA

发明人： ROMERO RAMOS CELSO RAUL ,  MARROQUIN QUELOPANA MIRYAM

IPC分类号： C12N15/70 ,  A61K39/00 ,  A61K39/385 ,  A61P37/04 ,  C12N15/62 ,  C12N15/68 ,  C12N15/69

CPC分类号： C12N15/70 ,  A61K39/0241 ,  A61K2039/53 ,  C07K14/195 ,  C07K2319/35 ,  C12N15/68 ,  C12N15/69

摘要： The present invention relates to a vector for the expression of recombinant proteins, antigens, pathogen-like particles and immunogenic complexes, said vector (pMRKA vector) being produced by modifying the plasmids containing the gene sequence of the T7 promoter of E. coli, this modification being mainly characterized by the substitution of the ampicillin-resistance gene by the kanamycin-resistance gene, and by the insertion of the par sequence (partition sequence which determines the efficient segregation of the plasmids in daughter cells during cell division). Also provided are expression vectors based on the pMRKA plasmid, which additionally comprise at least one of the gene sequences of the exosome of P. abyssi, which vectors are designated pMRKA-EXO, pMRKA-RING and pSUMAC. The invention also provides the vectors additionally comprising gene sequences with immunomodulatory or immunoregulatory activity, preferably the pMRKA-Z-Z-EXO and pMRKA-Z-Z-RING vectors. Other aspects of the invention include the method for producing said expression vectors and the use of the obtained vectors.

公开(公告)号：EP3077515A4

公开(公告)日：2017-05-24

申请号：EP14867094

申请日：2014-11-26

申请人： NAT UNIV SINGAPORE

发明人： TOO HENG PHON ,  ZOU RUIYANG

IPC分类号： C12N15/68 ,  C12N15/10 ,  C12N15/70

CPC分类号： C12N15/70 ,  C12N15/1093 ,  C12N15/68

公开(公告)号：EP2485760B1

公开(公告)日：2016-07-13

申请号：EP10766193.6

申请日：2010-10-08

申请人： Amgen, Inc ,  E. R. Squibb & Sons, L.L.C.

发明人： WILD, Kenneth, D. ,  TREANOR, James, J.s. ,  HUANG, Haichun ,  INOUE, Heather ,  ZHANG, Tie, J. ,  MARTIN, Frank

IPC分类号： A61K39/395 ,  C07K16/22 ,  G01N33/53 ,  C12N15/68 ,  C12N5/10 ,  A61P25/00 ,  A61P35/00 ,  A61P19/02 ,  A61P29/02

CPC分类号： C07K16/22 ,  A61K39/00 ,  A61K39/3955 ,  A61K2039/505 ,  A61K2039/54 ,  C07K2317/21 ,  C07K2317/56 ,  C07K2317/76

公开(公告)号：EP2814964A1

公开(公告)日：2014-12-24

申请号：EP13705106.6

申请日：2013-02-15

申请人： CureVac GmbH

发明人： THESS, Andreas ,  SCHLAKE, Thomas ,  PROBST, Jochen

IPC分类号： C12N15/68 ,  A61K39/00 ,  A61K39/35 ,  C12N15/117

CPC分类号： A61K39/0008 ,  A61K39/35 ,  A61K2039/53 ,  A61K2039/64 ,  C12N15/63 ,  C12N15/67 ,  C12N15/85

摘要： The present invention relates to a nucleic acid sequence, comprising or coding for a coding region, encoding at least one peptide or protein comprising an allergenic antigen or an autoimmune self-antigen or a fragment, variant or derivative thereof, at least one histone stem-loop and a poly(A) sequence or a polyadenylation signal. Furthermore the present invention provides the use of the nucleic acid for increasing the expression of said encoded peptide or protein. It also discloses its use for the preparation of a pharmaceutical composition, especially a vaccine, e.g. for use in the treatment of allergies or autoimmune diseases. The present invention further describes a method for increasing the expression of a peptide or protein comprising an allergenic antigen or an autoimmune self-antigen or a fragment, variant or derivative thereof, using the nucleic acid comprising or coding for a histone stem-loop and a poly(A) sequence or a polyadenylation signal.

公开(公告)号：EP2644701A1

公开(公告)日：2013-10-02

申请号：EP13001576.1

申请日：2013-03-27

申请人： Parks, Christopher L. ,  Wright, Kevin J. ,  Yuan, Maoli ,  Jurgens, Christy K. ,  Zhang, Xinsheng ,  Arban Domi ,  Hoffenberg, Simon ,  Chiuchiolo, Maria J. ,  Rabinovich, Svetlana ,  Wilson, Aaron J. ,  Lorenz, Ivo C.

发明人： Parks, Christopher L. ,  Wright, Kevin J. ,  Yuan, Maoli ,  Jurgens, Christy K. ,  Zhang, Xinsheng ,  Arban Domi ,  Hoffenberg, Simon ,  Chiuchiolo, Maria J. ,  Rabinovich, Svetlana ,  Wilson, Aaron J. ,  Lorenz, Ivo C.

IPC分类号： C12N15/67 ,  C12N15/68 ,  C07K14/005

CPC分类号： C12N15/68 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/5254 ,  A61K2039/5256 ,  C07K14/005 ,  C12N7/00 ,  C12N15/67 ,  C12N15/86 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2760/16034 ,  C12N2760/18443 ,  C12N2760/20243

摘要： The present invention relates to methods of developing gene inserts that are more compatible with the host vectors by modifying a protein sequence to lessen potential interference with vector propagation while ensuring that the protein is expressed and processed efficiently and maintains desired structural features, and designing a gene with a nucleotide sequence that resembles the base composition of the host vector genome.

摘要翻译： 本发明涉及通过修饰蛋白质序列来改变与宿主载体更相容的基因插入物的方法，以减少对载体繁殖的潜在干扰，同时确保蛋白质被有效表达和加工并保持所需的结构特征，并设计基因 其核苷酸序列类似于宿主载体基因组的基本组成。

公开(公告)号：EP2579900A1

公开(公告)日：2013-04-17

申请号：EP11724246.1

申请日：2011-06-10

申请人： Laboratorios del. Dr. Esteve, S.A. ,  Universitat Autònoma De Barcelona

发明人： BOSCH TUBERT, Fátima ,  AYUSO LÓPEZ, Éduard ,  RUZO MATÍAS, Albert

IPC分类号： A61K48/00 ,  C12N15/68 ,  C12N15/864 ,  C12N15/67 ,  C12N9/16 ,  C12N9/14

CPC分类号： C12N15/86 ,  A61K48/00 ,  A61K48/0066 ,  C12N9/14 ,  C12N15/52 ,  C12N15/8645 ,  C12N2750/14143 ,  C12N2830/008 ,  C12N2830/48 ,  C12N2840/105

摘要： The present invention provides new sequences, gene constructions, vectors and pharmaceutical compositions for the treatment of diseases and specially, for the treatment of mucopolysaccharidoses.

摘要翻译： 本发明提供了新的序列，基因构建，载体和用于治疗疾病的药物组合物，特别是用于治疗粘多糖贮积症。

公开(公告)号：EP2417260A1

公开(公告)日：2012-02-15

申请号：EP10713773.9

申请日：2010-04-08

申请人： Bristol-Myers Squibb Company

发明人： BASCH, Jonathan ,  FRANCESCHINI, Thomas ,  LIU, Suo, Win ,  CHIANG, Shu-jen

IPC分类号： C12N15/68 ,  C12N9/06 ,  C12N9/02

CPC分类号： C12N9/0008 ,  C12N9/0018

摘要： Bi-cistronic plasmids used for the expression of formate dehydrogenase (FDH) and modified phenylalanine dehydrogenase (PDHmod) are provided.